mSphere, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 19, 2024
Antibiotics save lives but can have unwanted effects on our gut microbes, thereby contributing to disease. A mechanistic understanding of how such microbes respond antibiotics is hence critical. Recently in
Language: Английский
Microorganisms, Journal Year: 2024, Volume and Issue: 12(2), P. 238 - 238
Published: Jan. 23, 2024
Gut luminal dysbiosis and pathobiosis result in compositional biodiversified alterations the microbial host co-metabolites. The primary mechanism of bacterial evolution is horizontal gene transfer (HGT), acquisition new traits can be achieved through exchange mobile genetic elements (MGEs). Introducing genetically engineered microbes (GEMs) might break harmonized balance intestinal compartment. present objectives are: 1. To reveal role played by GEMs’ transfers changing landscape enteric microbiome eubiosis 2. expand on potential detrimental effects those changes human genome health. A search articles published PubMed/MEDLINE, EMBASE, Scielo from 2000 to August 2023 using appropriate MeSH entry terms was performed. exchanges induce multiple diseases. GEMs change long-term natural pro- or eukaryotic cell inhabitants. worldwide regulatory authority’s safety control not enough protect public Viability, biocontainment, many other aspects are only partially controlled harmful consequences for health should avoided. It important remember that prevention most cost-effective strategy primum non nocere focus.
Language: Английский
Citations
9
Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)
Published: July 24, 2024
Abstract A major roadblock towards the realisation of a circular economy are lack high-value products that can be generated from waste. Black soldier flies (BSF; Hermetia illucens ) gaining traction for their ability to rapidly consume large quantities organic wastes. However, these primarily used produce small variety products, such as animal feed ingredients and fertiliser. Using synthetic biology, BSF could developed into novel sustainable biomanufacturing platform valorise broader waste feedstocks enhanced feeds, biomolecules including industrial enzymes lipids, improved
Language: Английский
Citations
8
Trends in Endocrinology and Metabolism, Journal Year: 2024, Volume and Issue: unknown
Published: May 1, 2024
The rising prevalence of metabolic diseases calls for innovative treatments. Peptide-based drugs have transformed the management conditions such as obesity and type 2 diabetes. Yet, challenges persist in oral delivery these peptides. This review explores potential 'advanced microbiome therapeutics' (AMTs), which involve engineered microbes peptides situ, thereby enhancing their bioavailability. Preclinical work on AMTs has shown promise treating animal models diseases, including obesity, diabetes, dysfunction-associated steatotic liver disease. Outstanding toward realizing improving peptide expression, ensuring predictable colonization control, stability, managing safety biocontainment concerns. Still, revolutionizing treatment potentially offering dynamic personalized novel therapeutic approaches.
Language: Английский
Citations
6
Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12
Published: Dec. 4, 2024
Over recent years, studies on microbiota research and synthetic biology have explored novel approaches microbial manipulation for therapeutic purposes. However, fragmented information is available this aspect with key insights scattered across various disciplines such as molecular biology, genetics, bioengineering, medicine. This review aims to the transformative potential of in advancing microbiome therapies, significant implications healthcare, agriculture, environmental sustainability. By merging computer science, engineering, allows precise design modification biological systems via cutting edge technologies like CRISPR/Cas9 gene editing, metabolic oligonucleotide synthesis, thus paving way targeted treatments personalized probiotics engineered microorganisms. The will also highlight vital role gut disorders caused by its dysbiosis suggesting microbiota-based therapies innovations biosensors real-time health monitoring, non-invasive diagnostic tools, automated bio foundries better outcomes. Moreover, challenges including genetic stability, safety, robust regulatory frameworks be discussed understand importance ongoing ensure safe effective interventions.
Language: Английский
Citations
6
DNA, Journal Year: 2024, Volume and Issue: 4(4), P. 427 - 454
Published: Nov. 14, 2024
Genetic engineering has revolutionized our ability to modify microorganisms for various applications in agriculture, medicine, and industry. This review examines recent advances genetic techniques bacteria, fungi, oomycetes, with a focus on CRISPR-Cas systems. In CRISPR-Cas9 enabled precise genome editing, enhancing antibiotic production metabolic engineering. For despite challenges associated their complex cell structures, CRISPR/Cas9 advanced the of enzymes secondary metabolites. significant plant pathogens, modified Agrobacterium-mediated transformation, CRISPR/Cas12a have contributed developing disease-resistant crops. provides comparative analysis efficiencies across these addresses ethical regulatory considerations. Future research directions include refining tools improve efficiency expand applicability non-model organisms. comprehensive overview highlights transformative potential microbiology its implications addressing global biotechnology.
Language: Английский
Citations
5
Biochemical Engineering Journal, Journal Year: 2024, Volume and Issue: 211, P. 109443 - 109443
Published: July 27, 2024
Language: Английский
Citations
4
ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: 13(9), P. 2684 - 2692
Published: Aug. 20, 2024
Advances in genome engineering and associated technologies have reinvigorated natural products research. Here we highlight the latest developments field across discover–design–build–test–learn cycle of bioengineering, from recent progress computational tools for AI-supported mining, enzyme pathway engineering, compound identification to novel host systems new techniques improving production levels, place these trends context responsible research innovation, emphasizing importance anticipatory analysis at early stages process development.
Language: Английский
Citations
4
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 9, 2025
Synthetic microbial consortia are collections of multiple strains or species engineered organisms living in a shared ecosystem. Because they can separate metabolic tasks among different strains, synthetic have myriad applications developing biomaterials, biomanufacturing, and biotherapeutics. However, often require burdensome control mechanisms to ensure that the members community remain at correct proportions. This is especially true continuous culture systems which slight differences growth rates lead extinctions. Here, we present simple method for controlling proportions using cross-feeding auxotrophic co-culture. We use mutually E. coli with essential gene deletions regulate consortium via missing nutrients each strain. demonstrate precise regulation co-culture steady-state ratio by exogenous addition nutrients. also model co-culture's behavior system ordinary differential equations enable us predict its response changes nutrient concentrations. Our work provides powerful tool proportion minimal costs constituent strains.
Language: Английский
Citations
0
Advanced Therapeutics, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 21, 2025
Abstract Recent advances in cancer treatments such as targeted therapy and immunotherapy, have brought hope for curing a variety of cancers. However, there are ongoing challenges poor targeting, biocompatibility biosafety. Engineered bacteria can cope with these problems, providing unique therapeutic approach the treatment tumors. Nanotechnology offers potential to modify surface bacteria, use biofilm coating technology physically encapsulate help escape host immune system improve efficiency safety drug delivery. Synthetic biology genetic engineering technologies treat “robotic factories” produce deliver anti‐cancer drugs, including anti‐tumor cytokines, immunomodulators, prodrug enzymes, so on, according clinical needs. therapies be used either monotherapy or combination other anticancer achieve better outcomes. In this review, it introduce summarize processing modification methods engineered therapy, analyze current trials therapy.
Language: Английский
Citations
0
Trends in Endocrinology and Metabolism, Journal Year: 2024, Volume and Issue: 35(8), P. 687 - 696
Published: April 17, 2024
Language: Английский
Citations
3