Optimization and analytical validation of the Allplex HPV28 genotyping assay for use in first-void urine samples DOI Creative Commons
Margo Bell, Iacopo Baussano, Carole Ho

et al.

Journal of Clinical Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 26, 2024

ABSTRACT Despite first-void urine (FVU) being increasingly recognized as a credible specimen for human papillomavirus (HPV) detection, there is lack of well-validated testing methods providing full quantitative genotyping required vaccine impact monitoring from FVU samples. The Allplex HPV28 assay, capable individually detecting 28 HPV genotypes, presents promising method. We aimed to evaluate its genotype-specific performance on samples, following optimization preanalytics. selected 701 samples collected using Colli-Pee device (20 mL, with UCM), enriched HPV-positivity ( n = 630) based previous GP5+/6+-PCR-based reverse line blot (GP5+/6+ RLB) and E7-MPG after Amicon filtration (AF). first evaluated the comparability agreement positivity according different Subsequently, we conducted comparison GP5+/6+ RLB AF AF. No significant differences in by were observed when comparing pre-centrifuged versus non-centrifuged DNA extraction, nor manual automated extraction. Good was between AF, slightly more sensitive all genotypes (average HPV28:GP5+/6+ ratio 1.729). Compared exhibited lower sensitivity 21 overlapping HPV28:E7-MPG 0.588). findings this study, combined practical considerations real-world implementation, support use or extraction without requirement pre-centrifugation, studies most notably those prevalence. IMPORTANCE This study provides analytical validation assay offering reliable, non-invasive, alternative cervical detection. It demonstrates validated approach that supports assay’s potential application settings, including low- middle-income countries, where non-invasive widely acceptable sampling are crucial maximizing population coverage representativity. Given urgent need accurate tools monitor vaccination impact, these offer timely impactful contribution field.

Language: Английский

DNA methylation as a triage tool for cervical cancer screening – A meeting report DOI Creative Commons
F. Ricardo Burdier, Dur-e-Nayab Waheed, Belinda Nedjai

et al.

Preventive Medicine Reports, Journal Year: 2024, Volume and Issue: 41, P. 102678 - 102678

Published: March 6, 2024

DNA methylation is proposed as a novel biomarker able to monitor molecular events in human papillomavirus (HPV) infection pathophysiology, enabling the distinction between HPV-induced lesions with regression potential from those that may progress HPV-related cancer.

Language: Английский

Citations

13
2023 global inventory of commercial molecular tests for human papillomaviruses (HPV) DOI Creative Commons
Mario Poljak, Anja Oštrbenk Valenčak, Kate Cuschieri

et al.

Journal of Clinical Virology, Journal Year: 2024, Volume and Issue: 172, P. 105671 - 105671

Published: March 14, 2024

To suit the needs of human papillomaviruses (HPV) community comprehensively, a range commercial HPV tests with different performance characteristics are required. Four periodic inventories molecular present in global market were published previously 2010, 2012, 2015 and 2020. For fifth inventory, data retrieved from internal files detailed search using main bibliographic databases as well general internet without period or language restrictions was performed December 2023. At least 264 distinct (and 511 test variants) available globally A small 2020-2023 net increase total numbers observed, but strong introduction/withdrawal dynamic: 86 new 141 introduced 76 55 withdrawn last four years. Although quality improvement some recorded, half all still single peer-reviewed publication, 79 % evidence that demonstrate line requirements agreed community. Only relatively pool fulfill operational/performance required to meet cervical cancer screening challenge. clinical analytical many largely unknown, such used worldwide daily practice research, potentially deleterious consequences. Due this long-lasting unfavorable situation, significant scope for persists both manufacturers

Language: Английский

Citations

13
Clinical and Analytical Evaluation of the Abbott Alinity m HR HPV Assay in a New Generation First‐Void Urine Collector DOI
Severien Van Keer, Laura Téblick, Gilbert Donders

et al.

Journal of Medical Virology, Journal Year: 2025, Volume and Issue: 97(2)

Published: Feb. 1, 2025

Urine-based self-sampling approaches can simplify cervical screening programs whilst increasing response. This study reports on the performance of Abbott Alinity m HR HPV urine, self-collected at home using a new generation first-void urination device that is suitable for postal delivery (Novosanis Colli-Pee Small Volumes). First-void urine and paired samples from 297 females attending colposcopy (age 25-65, NCT04530201) were analysed presence Human Papillomavirus (HPV) DNA. Cervical disease was confirmed by and/or histology. testing less sensitive high-grade intraepithelial neoplasia (CIN2 +; ratio 0.91; 95% CI: 0.83-0.99), though equally specific (< CIN2; 1.04; 0.92-1.19) compared to manufacturer established cut-off samples. Adjusting improved sensitivity CIN2+ (ratio 0.96; 0.90-1.03), maintaining equal specificity 1.00; 0.88-1.14). Cohen's kappa agreements outcomes between sample pairs good excellent both cut-offs (range: 0.64-0.85). Using test's adjusted cutoff no difference in clinical or observed These data highlight importance evaluating self-sample-specific assays, previously validated

Language: Английский

Citations

1
Equivalent clinical accuracy of HPV DNA testing using cobas 4800 and 6800 HPV Systems in paired urine and cervical samples DOI
Severien Van Keer, Ardashel Latsuzbaia, Davy Vanden Broeck

et al.

Journal of Molecular Diagnostics, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

1
Menstrual Blood as a Diagnostic Specimen for Human Papillomavirus Genotyping and Genital Tract Infection Using Next-Generation Sequencing as a Novel Diagnostic Tool DOI Creative Commons
Hin Fung Tsang, Yui-Shing Cheung, Allen Chi-Shing Yu

et al.

Diagnostics, Journal Year: 2024, Volume and Issue: 14(7), P. 686 - 686

Published: March 25, 2024

Background: Menstrual blood (MB) is a convenient specimen type that can be self-collected easily and non-invasively by women. This study assessed the potential application of MB as diagnostic to detect genital tract infections (GTIs) human papillomavirus (HPV) in Method: Genomic DNA was extracted from samples. Pacific Bioscience (Pacbio) 16S ribosomal (rDNA) high-fidelity (HiFi) long-read sequencing HPV PCR were performed. Results: samples collected women with pathological diagnosis CIN1, CIN2, CIN3 or infection. The sensitivity positive predictive value (PPV) high-risk detection using found 66.7%. A shift vaginal flora significant depletion Lactobacillus spp. microbiota communities observed rDNA sequencing. Conclusions: In this study, we demonstrated proper consideration for non-invasive genotyping GTIs metagenomic next-generation (mNGS). testing suitable all who menstruate has opened up possibility use maintain women’s health.

Language: Английский

Citations

4
Oral human papillomavirus (HPV) prevalence and genotyping among healthy adult populations in the United States and Europe: results from the PROGRESS (PRevalence of Oral hpv infection, a Global aSSessment) study DOI Creative Commons
Laia Alemany, Marisa Felsher, Anna R. Giuliano

et al.

EClinicalMedicine, Journal Year: 2025, Volume and Issue: 79, P. 103018 - 103018

Published: Jan. 1, 2025

Language: Английский

Citations

0
The viral landscape in metastatic solid cancers DOI Creative Commons
Robin Mjelle, Ícaro Maia Santos de Castro, Kristin Roseth Aass

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(4), P. e42548 - e42548

Published: Feb. 1, 2025

Here, we analyze the viral landscape in blood and tissue from 4,918 metastatic cancer patients across 38 solid types Hartwig Medical Foundation (HMF) cohort, largest pan-cancer study on cancer. Using a coverage-based filtering approach, detected 25 unique genera 32 different types, with total of 747 virus-positive samples. We 336 samples 29 dominated by Torque teno virus Alphatorquevirus. The were Alphapapillomavirus Roseolovirus. was significantly enriched genital, anal, colorectal cancers associated host mutational signatures transcriptional programs related to immunity DNA repair. Host genes integration tended be more highly expressed HPV had higher somatic mutation rates number extrachromosomal elements. also significant proportion cervix anal cancers, suggesting potential blood-based biomarker.

Language: Английский

Citations

0
Smartphone-based urine colourimetric assay for home self-screening of HPV infection DOI
Xiaoli Zhang, Zhaoguang Wang, Jing Yang

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: 334, P. 125923 - 125923

Published: Feb. 19, 2025

Language: Английский

Citations

0
Addressing Global Disparities in Cervical Cancer Burden: A Narrative Review of Emerging Strategies DOI Creative Commons

K. Gopalkrishnan,

Roksana Karim

Current HIV/AIDS Reports, Journal Year: 2025, Volume and Issue: 22(1)

Published: Feb. 21, 2025

Cervical cancer burden is disproportionately higher in low to middle income countries, especially countries with a high human immunodeficiency virus (HIV) burden. This review investigates barriers implementation and assesses current progress cervical screening lower resource settings by reviewing technologies strategies that have already been implemented countries. Several novel innovations embrace the recent World Health Organization (WHO) update guidelines recommends "screen treat" approach rather than "screen, triage approach. Innovations include papillomavirus (HPV) self-sampling, portable visualization devices, creative large-scale approaches increase accessibility. Overall, low-cost, accurate, point-of-care test could alleviate most of associated settings. Further research into development low-cost HPV conjunction vaccine other tools expedite progress.

Language: Английский

Citations

0
Salivary Biomarkers Identification: Advances in Standard and Emerging Technologies DOI Creative Commons

Vlad Denis Constantin,

Ionuț Luchian, Ancuța Goriuc

et al.

Oral, Journal Year: 2025, Volume and Issue: 5(2), P. 26 - 26

Published: April 9, 2025

Introduction: Salivary biomarkers have been extensively studied in relation to oral disease, such as periodontal cancer, and dental caries, well systemic conditions including diabetes, cardiovascular diseases, neurological disorders. Literature Review: A systematic literature review was conducted, analyzing recent advancements salivary biomarker research. Databases PubMed, Scopus, Web of Science were searched for relevant studies published the last decade. The selection criteria included focusing on identification, validation, clinical application diagnosing diseases. Various detection techniques, enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), mass spectrometry, biosensor technologies, reviewed assess their effectiveness analysis. Specific biomarkers, inflammatory cytokines, oxidative stress markers, microRNAs, identified reliable indicators disease progression. Current Trends Future Perspectives: Advances proteomics, genomics, metabolomics significantly enhanced ability analyze with high sensitivity specificity. Despite promising findings, challenges remain standardizing sample collection, processing, analysis ensure reproducibility applicability. Conclusions: research should focus developing point-of-care diagnostic tools integrating artificial intelligence improve predictive accuracy biomarkers.

Language: Английский

Citations

0