Conjugal plasmid transfer in the plant rhizosphere in the One Health context DOI Creative Commons
Francesco Riva, Arnaud Dechesne, Ester M. Eckert

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 29, 2024

Introduction Horizontal gene transfer (HGT) of antibiotic resistance genes (ARGs) is one the primary routes antimicrobial (AMR) dissemination. In One Health context, tracking spread mobile genetic elements (MGEs) carrying ARGs in agri-food ecosystems pivotal understanding AMR diffusion and estimating potential risks for human health. So far, little attention has been devoted to plant niches; hence, this study aimed evaluate conjugal bacterial community associated with rhizosphere, a hotspot microbial abundance activity soil. We simulated source determinants that could enter food chain via plants through irrigation. Methods Among strains isolated from treated wastewater, strain Klebsiella variicola EEF15 was selected as an ARG donor because relevance Enterobacteriaceae context framework. The ability recolonize lettuce, chosen model vegetables were consumed raw, assessed by rifampicin resistant mutant. K. genetically manipulated track broad host range plasmid pKJK5 containing fluorescent marker natural rhizosphere microbiome obtained lettuce plants. Transconjugants sorted protein expression identified 16S rRNA amplicon sequencing. Results discussion able colonize inhabit its leaf endosphere 7 days past administration. Fluorescence stereomicroscopy revealed at frequency 10 −3 ; cell sorting allowed selection transconjugants. conjugation rates strain’s make EEF15:: lacI q -pLpp-mCherry-gm R pKJK5::Plac:: gfp interesting candidate ecosystem. Future studies taking advantage additional environmental provide comprehensive snapshot context.

Language: Английский

Update on novel validly published taxa of bacteria isolated from domestic animals described in 2022 DOI
Sara D. Lawhon, Claire R. Burbick, Erik Munson

et al.

Journal of Clinical Microbiology, Journal Year: 2023, Volume and Issue: 61(12)

Published: Oct. 27, 2023

ABSTRACT Expansion of our knowledge the microbial world continues to progress at a rapid rate and carries with it an associated need for recognizing understanding implications those changes. Here, we describe additions novel taxa from domestic animals published in 2022 that are validly per International Code Nomenclature Prokaryotes. These included new members Staphylococcaceae , Moraxella nasovis sp. nov. sheep respiratory disease, three Campylobacteraceae (including one chickens spotty liver disease), multiple organisms microbiota dogs, pigs, especially honeybees other important pollinators. Noteworthy were diseases cattle, including mastitis, endocarditis, orchitis, endometritis. Also described was Pseudochrobactrum algeriense nov., member Brucellaceae family, isolated mammary lymph nodes cows.

Language: Английский

Citations

14
Metal concentration in freshwater sediments is linked to microbial biodiversity and community composition DOI Creative Commons
Mutai Bao, Ahmed Tlili, Kristin Schirmer

et al.

Environment International, Journal Year: 2025, Volume and Issue: unknown, P. 109465 - 109465

Published: April 1, 2025

The effect of metals on freshwater microbiomes is poorly understood compared to other factors, such as nutrients or climate. While deleterious effects plant and animal biodiversity are well documented, the role in shaping biodiversity, composition functional potential sediment microbial communities remains unknown. Therefore, we explored if metal concentrations can be linked alterations communities. We collected sediments from 34 streams lakes Switzerland grouped them based their content. Microbial diversity community were determined using 16S rRNA gene amplicon sequencing. Most not contaminated with according Sediment Environmental Quality Criteria, although some stations exceeded limits for Cu, Zn, Pb. Nevertheless, correlational analysis indicated links various aspects biodiversity. Al significantly (p < 0.05) correlated richness. further observed a predominantly negative correlation between abundances dominant taxa. Predicted that different types have potential. For example, Mn exhibited significant positive nitrogen fixation potential, whereas Pb, Zn displayed correlation. Overall, our findings indicate may alter even at ambient concentrations. Further research into drivers factors loss warranted.

Language: Английский

Citations

0
An update on novel taxa and revised taxonomic status of bacteria isolated from non-domestic animals described in 2022 DOI
Claire R. Burbick, Sara D. Lawhon, Erik Munson

et al.

Journal of Clinical Microbiology, Journal Year: 2023, Volume and Issue: 61(12)

Published: Oct. 27, 2023

ABSTRACT Numbers of new and revised microbial taxa are continuously expanding, the rapid accumulation novel bacterial species is challenging to keep up with in best circumstances. With that mind, following template reports on prokaryotic isolated from humans, this now second publication summarizing non-domestic animal Journal Clinical Microbiology . The majority were obtained as part programs identify bacteria mucosal surfaces gastrointestinal tract healthy wildlife. A few notable included Erysipelothrix spp. mammalian aquatic sources a Bartonella rodent, both which could be considered members emerging re-emerging genera pathogenic potential humans animals.

Language: Английский

Citations

5
An Update on Novel Taxa and Revised Taxonomic Status of Bacteria Isolated from Domestic Animals Described in 2018 to 2021 DOI
Erik Munson, Sara D. Lawhon, Claire R. Burbick

et al.

Journal of Clinical Microbiology, Journal Year: 2022, Volume and Issue: 61(2)

Published: Dec. 19, 2022

Novel bacterial taxonomy and nomenclature revisions can have significant impacts on clinical practice, disease epidemiology, veterinary microbiology laboratory operations. Expansion of research the microbiota humans, animals, insects has potential organisms interest. Implications taxonomic changes may be especially important when considering zoonotic diseases. Here, we address novel significance. Noteworthy discussion centers around descriptions mastitis pathogens in

Language: Английский

Citations

8
Valid and accepted novel bacterial taxa isolated from non-domestic animals and taxonomic revisions published in 2023 DOI
Erik Munson, Claire R. Burbick, Sara D. Lawhon

et al.

Journal of Clinical Microbiology, Journal Year: 2024, Volume and Issue: 62(10)

Published: Oct. 1, 2024

Continued investigation into the bacteria associated with non-domestic animals provides important information for recognizing normal flora, assessing health status of these unique species animals, and identifying new or emerging pathogens concern. In this summary novel taxa taxonomic revisions, considerable additions have been made toward understanding fecal mucosal flora in multiple wild animal species. addition, pathogenic are discussed, including

Language: Английский

Citations

1
An update on novel taxa and revised taxonomic status of bacteria isolated from aquatic host species described in 2022–2023 DOI
Claire R. Burbick, Sara D. Lawhon,

Brittany Bukouras

et al.

Journal of Clinical Microbiology, Journal Year: 2024, Volume and Issue: 62(11)

Published: Oct. 24, 2024

ABSTRACT The description of new taxa and nomenclature updates to currently known from aquatic animal species continues. After a review the literature 2022 2023, multiple lists bacteria, including members Phylum Planctomycetota , were compiled. As with previous review, most bacteria are oxidase-positive Gram-negative bacilli familiar families in Aeromonadaceae Flavobacteriaceae Vibrionaceae . A number Gram-positive described Nocardioides Paenibacillus Streptomyces genera. Two anaerobic listed, one member Family Planctomycetaceae is noted. Revised briefly mentioned. majority revised isolated healthy animals, therefore, role these health disease unknown. Bacteria pathogenic association potential production bioactive substances highlighted.

Language: Английский

Citations

1
An update on novel taxa and revised taxonomic status of bacteria isolated from domestic companion and agricultural animals described in 2023 DOI
Sara D. Lawhon, Claire R. Burbick,

Trinity Krueger

et al.

Journal of Clinical Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 4, 2024

ABSTRACT With the proliferation of abundant bacterial genomic data comes recognition new organisms as well a better understanding relatedness known bacteria. Recognizing associated taxonomic changes enhances communication and about significance novel deeper pathogens. This review addresses addition multiple gastrointestinal bacteria that form normal microbiota in variety animals including honeybees from domestic an alpha-hemolytic Streptococcus species guinea pigs, two Moraxella spp. cows goats, Capnocytophaga cats, thermophilic Campylobacter Exercitatus genus Family Pasteurellaceae . Several revisions to nomenclature also appeared 2023 change Clostridium spiroforme, which causes anorexia diarrhea rabbits, Thomasclavelia spiroformis comb. nov. Mannheimia ovis pernigra

Language: Английский

Citations

1
Update on Novel Taxa and Revised Taxonomic Status of Bacteria Isolated from Nondomestic Animals Described in 2018 to 2021 DOI
Sara D. Lawhon, Claire R. Burbick, Erik Munson

et al.

Journal of Clinical Microbiology, Journal Year: 2022, Volume and Issue: 61(2)

Published: Dec. 19, 2022

Revisions and new additions to bacterial taxonomy can have a significant widespread impact on clinical practice, infectious disease epidemiology, veterinary microbiology laboratory operations, wildlife conservation efforts. The expansion of genome sequencing technologies has revolutionized our knowledge the microbiota humans, animals, insects. Here, we address novel nomenclature revisions significance that bacteria isolated from nondomestic wildlife, with emphasis being placed are associated in their hosts or were host animal species culturally significant, target efforts, serve as reservoirs for human pathogens.

Language: Английский

Citations

7
A new OCH β-lactamase from a Brucella pseudintermedia (Ochrobactrum pseudintermedium) strain isolated from Zophobas morio larvae DOI Creative Commons
Cindy Kundlacz,

Claudia Aldeia,

Yasmine Eddoubaji

et al.

Journal of Global Antimicrobial Resistance, Journal Year: 2023, Volume and Issue: 36, P. 65 - 69

Published: Dec. 20, 2023

OCH class C β-lactamases have been reported in several species belonging to the Brucella genus that were formerly known as Ochrobactrum. Moreover, only one complete genome of pseudintermedia has published. In this work, we describe a B. strain possessing new bla

Language: Английский

Citations

2
Conjugal plasmid transfer in the plant rhizosphere in the One Health context DOI Creative Commons
Francesco Riva, Arnaud Dechesne, Ester M. Eckert

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 29, 2024

Introduction Horizontal gene transfer (HGT) of antibiotic resistance genes (ARGs) is one the primary routes antimicrobial (AMR) dissemination. In One Health context, tracking spread mobile genetic elements (MGEs) carrying ARGs in agri-food ecosystems pivotal understanding AMR diffusion and estimating potential risks for human health. So far, little attention has been devoted to plant niches; hence, this study aimed evaluate conjugal bacterial community associated with rhizosphere, a hotspot microbial abundance activity soil. We simulated source determinants that could enter food chain via plants through irrigation. Methods Among strains isolated from treated wastewater, strain Klebsiella variicola EEF15 was selected as an ARG donor because relevance Enterobacteriaceae context framework. The ability recolonize lettuce, chosen model vegetables were consumed raw, assessed by rifampicin resistant mutant. K. genetically manipulated track broad host range plasmid pKJK5 containing fluorescent marker natural rhizosphere microbiome obtained lettuce plants. Transconjugants sorted protein expression identified 16S rRNA amplicon sequencing. Results discussion able colonize inhabit its leaf endosphere 7 days past administration. Fluorescence stereomicroscopy revealed at frequency 10 −3 ; cell sorting allowed selection transconjugants. conjugation rates strain’s make EEF15:: lacI q -pLpp-mCherry-gm R pKJK5::Plac:: gfp interesting candidate ecosystem. Future studies taking advantage additional environmental provide comprehensive snapshot context.

Language: Английский

Citations

0