Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)
Published: Oct. 1, 2024
Language: Английский
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: Nov. 2, 2024
SARS-CoV-2 initiates infection in the conducting airways, where mucociliary clearance inhibits pathogen penetration. However, it is unclear how impacts spread after established. To investigate viral at this site, we perform live imaging of infected differentiated primary human bronchial epithelium cultures for up to 12 days. Using a fluorescent reporter virus and markers cilia mucus, longitudinally monitor mucus motion, ciliary infection. Infected cell numbers peak 4 days post infection, forming characteristic foci that tracked movement. Inhibition MCC using physical genetic perturbations limits foci. Later deteriorates. Increased secretion accompanies motion defects, but vectorial resume removal, suggesting may mediate deterioration. Our work shows while can facilitate initial subsequent decreases inhibit spread, revealing complex interplay between MCC.
Language: Английский
Citations
4
Current Opinion in Virology, Journal Year: 2025, Volume and Issue: 70, P. 101452 - 101452
Published: Feb. 1, 2025
Language: Английский
Citations
0
Molecular Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown
Published: March 26, 2025
To understand requirements for immunization via the oral mucosa, an in vitro model that recapitulates physical barrier of mouth, allows quantification antigen uptake and permeability mounts inflammatory response to adjuvant is needed. The structure 4 models human mucosa was determined by histochemical staining transepithelial electrical resistance (TEER) measurements. A TR146 based air-liquid interface (ALI) most closely mimicked vivo conditions. This confirmed validation studies using dextran caffeine as diffusant molecules. Apparent coefficients (Papp) adenovirus (Ad) adeno-associated virus (AAV) this were 4.3 × 10-13 2.2 10-10 respectively, while 100% total dose H1N1 influenza remained epithelial layer. Sodium glycocholate a hyperosmotic formulation improved amount Ad (p = 0.02) AAV 0.003) entered epithelium, respectively. Significant amounts IL-6 (45.1 pg/mL), GM-CSF (94.7 pg/mL) IFN-γ (4.3 produced infection. Treatment with AS03-like induced production (34.9 TNF-∝ (43 (121.2 (14.1 pg/mL). highlights contribution differentiated cells immune vaccines adjuvants.
Language: Английский
Citations
0
Asian Journal of Chemistry, Journal Year: 2025, Volume and Issue: 37(4), P. 745 - 757
Published: March 29, 2025
The application of click chemistry in the synthesis intricate carbohydrate derivatives has revolutionized domains chemical biology and synthetic chemistry. This review examines function chemistry, namely copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC), effectively altering structures producing practical glycoconjugates. Carbohydrates are essential several biological functions, such as cellular recognition immunological responses, however, their complicated structure makes production difficult. Click provides an optimal answer to these issues due its simplicity, high yields bioorthogonality. methodology been extensively used advancement glycoproteins, glycodendrimers glycopeptides, which play a crucial role fields drug discovery, vaccine development diagnostics. In addition, facilitated biodegradable polymers glycomacromolecules that have potential be materials science, for sustainable smart technology. flexibility accuracy CuAAC carbohydrate-based new opportunities investigation, stimulating advancements both biomedicine industrial applications. evaluation also emphasizes capacity expedite multifunctional therapeutic drugs target ailments like cancer.
Language: Английский
Citations
0
Viruses, Journal Year: 2024, Volume and Issue: 16(6), P. 883 - 883
Published: May 30, 2024
Influenza A viruses continue to be a serious health risk people and result in large-scale socio-economic loss. Avian influenza typically do not replicate efficiently mammals, but through the accumulation of mutations or genetic reassortment, they can overcome interspecies barriers, adapt new hosts, spread among them. Zoonotic sporadically infect humans exhibit limited human-to-human transmission. However, further adaptation these may airborne transmissible with pandemic potential. Therefore, we are beginning understand changes mechanisms that influence interspecific adaptation, cross-species transmission, potential viruses. We also discuss phenotypic traits associated transmission order provide theoretical guidance for surveillance strains prevention pandemics.
Language: Английский
Citations
2
Veterinary Research, Journal Year: 2024, Volume and Issue: 55(1)
Published: Sept. 20, 2024
Language: Английский
Citations
2
Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)
Published: Oct. 1, 2024
Language: Английский
Citations
1