Overexpression of transcriptional regulator and tailoring enzyme leads to the discovery of anti-inflammatory meroterpenoids from marine-derived fungus Alternaria alternata JJY-32 DOI Creative Commons
Guowei Liu, Dan Liu,

Zongyuan Li

et al.

Frontiers in Marine Science, Journal Year: 2022, Volume and Issue: 9

Published: Oct. 28, 2022

Four new ACTG-toxins like meroterpenoids tricycloalternarenes O-R were discovered from a marine-derived fungus Alternaria alternata JJY-32 by the overexpression of LaeA-like global transcriptional regulator and MpaB-like biosynthetic tailoring enzyme. Among those structures, tricycloalternarene O possesses non-canonical cyclohexanone-pyran fused furan ring structure R contains truncated isoprenoid terminal. The structures elucidated based on comprehensive 1D 2D NMR data assisted ECD calculations. Tricycloalternarenes showed potent anti-inflammatory activity in TLR4-transfected microphage cells (RAW264.7).

Language: Английский

Aabrm1-mediated melanin synthesis is essential to growth and development, stress adaption, and pathogenicity in Alternaria alternata DOI Creative Commons
Rong Li, Yongcai Li,

Wenyi Xu

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 11, 2024

Scytalone dehydratase (brm1) is one of the key enzymes in 1, 8-dihydroxynaphthalene (DHN) melanin synthesis, which mediates biosythesis and regulates cell biological process plant fungi, but its function Alternaria alternata , causal agent pear black spot, unclear. Brm1 A. was cloned, identified, named as Aabrm1 . An -deletion mutant generated revealed that deletion leads to a significant decrease production forms orange colony smooth spores. In addition, gene impaired infection structure information penetration. The external stress resistance Δ significantly weakened, and, particular, it very sensitive oxidative stress, contents H 2 O .- were increased. Virulence reduced non-wound-inoculated leaves not changed wound-inoculated fruit. These results indicated Aabrm1- mediated synthesis plays an important role pathogenicity alternata.

Language: Английский

Citations

1
Transcriptome Analysis Reveals Candidate Genes for Light Regulation of Elsinochrome Biosynthesis in Elsinoë arachidis DOI Creative Commons
Dan Liu,

Jingzi Piao,

Li Yang

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(5), P. 1027 - 1027

Published: May 19, 2024

Light regulation is critical in fungal growth, development, morphogenesis, secondary metabolism, and the biological clock. The fungus Elsinoë arachidis known to produce mycotoxin Elsinochrome (ESC), a key factor contributing its pathogenicity, under light conditions. Although previous studies have predominantly focused on light-induced production of ESC biosynthetic pathways, detailed mechanisms underlying this process remain largely unexplored. This study explores influence gene expression E. arachidis. Under white exposure for 28 days, yield was observed reach 33.22 nmol/plug. Through transcriptome analysis, 5925 genes were identified as differentially expressed between dark conditions, highlighting significant impact expression. Bioinformatics specific light-regulated genes, including eight photoreceptor five global regulatory factors, cluster 12 directly involved biosynthesis, with trends confirmed by RT-qPCR. In conclusion, reveals substantial alteration associated biosynthesis identifies potential candidates in-depth functional analysis. These findings advance understanding suggest new strategies pathogenicity control.

Language: Английский

Citations

1
Identification of a biosynthetic gene cluster for a red pigment cristazarin produced by a lichen-forming fungus Cladonia metacorallifera DOI Creative Commons
Jaycee Augusto Paguirigan, Jung A Kim, Jae‐Seoun Hur

et al.

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(6), P. e0287559 - e0287559

Published: June 23, 2023

Lichens are known to produce many novel bioactive metabolites. To date, approximately 1,000 secondary metabolites have been discovered, which predominantly produced by the lichen mycobionts. However, despite extensive studies on production of metabolites, little is about responsible biosynthetic gene clusters (BGCs). Here, we identified a putative BGC that implicated in red pigment, cristazarin (a naphthazarin derivative), Cladonia metacorallifera. Previously, was shown be specifically induced growth media containing fructose as sole carbon source. Thus, performed transcriptome analysis C. metacorallifera growing different sources including identify for cristazarin. Among 39 polyketide synthase (PKS) genes found genome metacorallifera, non-reducing PKS (coined crz7) highly expressed either or glucose. The borders cluster were delimited co-expression patterns neighboring crz7. BGCs conserved also borealis and macilenta, indicating these related species metabolic potentials Phylogenetic revealed Crz7 sister fungal PKSs biosynthesize an acetylated tetrahydoxynaphthalene precursor melanin pigment. Based phylogenetic placement functions its genes, proposed plausible route In this study, lichen-specific likely involved biosynthesis derivative, cristazarin, confirmed profiling under inducing non-inducing conditions effective strategy linking interest genes.

Language: Английский

Citations

3
Characterization of Fungal Melanins from Black Stains on Paper Artefacts DOI Creative Commons

D. C. Melo,

Tiago G. Paiva, João A. Lopes

et al.

Heritage, Journal Year: 2022, Volume and Issue: 5(4), P. 3049 - 3065

Published: Oct. 11, 2022

Melanins play a fundamental role in the biology and ecology of several fungal species. Unfortunately, this group amorphous macromolecules also severely (and most times irreversibly) stains cultural heritage objects. Despite efforts made throughout years, knowledge chemical composition structure melanins is still insufficient, which hampers task safely cleaning these colourants from materials targeted way without causing further deterioration. This work aimed therefore to contribute towards enlightening characteristics three fungi that are common paper colonizers: Aspergillus niger, Chaetomium globosum Cladosporium cladosporioides. The extracted were characterized by FTIR, Raman, UV-vis, Solid-State NMR MALDI-TOF MS spectroscopies effect inhibitors DHN-melanin DOPA-melanin pathways on colony pigmentation growth was evaluated. Although all show predominantly aromatic with carbonyl phenolic groups, some differences between can be highlighted. obtained Ch. Cl. cladosporioides exhibited similar structures both presented characteristics, while A. niger’s revealed more complex ordered structure, higher prevalence highly conjugated carbonyls than others, besides additional presence yellow/green component. These conclusions cannot overlooked selecting methodologies for melanin materials.

Language: Английский

Citations

4
Overexpression of transcriptional regulator and tailoring enzyme leads to the discovery of anti-inflammatory meroterpenoids from marine-derived fungus Alternaria alternata JJY-32 DOI Creative Commons
Guowei Liu, Dan Liu,

Zongyuan Li

et al.

Frontiers in Marine Science, Journal Year: 2022, Volume and Issue: 9

Published: Oct. 28, 2022

Four new ACTG-toxins like meroterpenoids tricycloalternarenes O-R were discovered from a marine-derived fungus Alternaria alternata JJY-32 by the overexpression of LaeA-like global transcriptional regulator and MpaB-like biosynthetic tailoring enzyme. Among those structures, tricycloalternarene O possesses non-canonical cyclohexanone-pyran fused furan ring structure R contains truncated isoprenoid terminal. The structures elucidated based on comprehensive 1D 2D NMR data assisted ECD calculations. Tricycloalternarenes showed potent anti-inflammatory activity in TLR4-transfected microphage cells (RAW264.7).

Language: Английский

Citations

3