A portrait of the infected cell: how SARS-CoV-2 infection reshapes cellular processes and pathways DOI Creative Commons

Valentina Marano,

Štěpánka Vlachová,

Sofia Maria Luigia Tiano

et al.

npj Viruses, Journal Year: 2024, Volume and Issue: 2(1)

Published: Dec. 17, 2024

Positive-sense single-stranded RNA (+ssRNA) viruses exert a profound influence on cellular organelles and metabolic pathway by usurping host processes to promote their replication. In this review, we present portrait of selected pathways perturbed in SARS-CoV-2 infection: the effect viral translation, replication assembly morphology function ER, remodelling degradative with focus autophagic processes, alterations affecting membranes lipid metabolism. For each these highlight specific factors involved interplay microscopic tug-of-war between pro-viral anti-viral effects that ultimately tip scale toward propagation or resolution infection.

Language: Английский

Endoplasmic reticulum stress in diseases DOI Creative Commons
Yingying Liu, Chunling Xu, Renjun Gu

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(9)

Published: Aug. 26, 2024

The endoplasmic reticulum (ER) is a key organelle in eukaryotic cells, responsible for wide range of vital functions, including the modification, folding, and trafficking proteins, as well biosynthesis lipids maintenance intracellular calcium homeostasis. A variety factors can disrupt function ER, leading to aggregation unfolded misfolded proteins within its confines induction ER stress. conserved cascade signaling events known protein response (UPR) has evolved relieve burden restore However, these processes culminate cell death while stress sustained over an extended period at elevated levels. This review summarizes potential role UPR determining fate various diseases, cardiovascular neurodegenerative metabolic autoimmune fibrotic viral infections, cancer. It also puts forward that manipulation this intricate pathway may represent novel target drug discovery innovative therapeutic strategies context human diseases.

Language: Английский

Citations

11
Human Betacoronavirus OC43 Interferes with the Integrated Stress Response Pathway in Infected Cells DOI Creative Commons
Stacia M. Dolliver,

Caleb Galbraith,

Denys A. Khaperskyy

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(2), P. 212 - 212

Published: Jan. 31, 2024

Viruses evolve many strategies to ensure the efficient synthesis of their proteins. One such strategy is inhibition integrated stress response—the mechanism through which infected cells arrest translation phosphorylation alpha subunit eukaryotic initiation factor 2 (eIF2α). We have recently shown that human common cold betacoronavirus OC43 actively inhibits eIF2α in response sodium arsenite, a potent inducer oxidative stress. In this work, we examined modulation responses by and demonstrated negative feedback regulator GADD34 strongly induced cells. However, upregulation expression was independent from activation not required for virus-infected Our work reveals complex interplay between coronavirus response, viral protein ensured but loop disrupted.

Language: Английский

Citations

4
Insights into the Activation of Unfolded Protein Response Mechanism during Coronavirus Infection DOI Creative Commons
Panagiotis Κeramidas,

Maria Pitou,

Eleni Papachristou

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(5), P. 4286 - 4308

Published: May 5, 2024

Coronaviruses represent a significant class of viruses that affect both animals and humans. Their replication cycle is strongly associated with the endoplasmic reticulum (ER), which, upon virus invasion, triggers ER stress responses. The activation unfolded protein response (UPR) within infected cells performed from three transmembrane receptors, IRE1, PERK, ATF6, results in reduction production, boost ER’s ability to fold proteins properly, initiation ER-associated degradation (ERAD) remove misfolded or proteins. However, cases prolonged severe stress, UPR can also instigate apoptotic cell death inflammation. Herein, we discuss ER-triggered host responses after coronavirus infection, as well pharmaceutical targeting potential antiviral strategy.

Language: Английский

Citations

4
4D-DIA Proteomics Uncovers New Insights into Host Salivary Response Following SARS-CoV-2 Omicron Infection DOI Creative Commons
Iasmim Lopes de Lima, Thaís Regiani Cataldi, Carlos Brites

et al.

Journal of Proteome Research, Journal Year: 2025, Volume and Issue: 24(2), P. 499 - 514

Published: Jan. 13, 2025

Since late 2021, Omicron variants have dominated the epidemiological scenario as most successful severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sublineages, driving new and breakthrough infections globally over past two years. In this study, we investigated for first time host salivary response of COVID-19 patients infected with (BA.1, BA.2, BA.4/5) by using an untargeted four-dimensional data-independent acquisition (4D-DIA)-based proteomics approach. We identified 137 proteins whose abundance levels differed between positive negative groups. Salivary signatures were mainly enriched in ribosomal proteins, linked to mRNAviral translation, protein synthesis processing, immune innate, antiapoptotic signaling. The higher 14-3-3 (YWHAG, YWHAQ, YWHAE, SFN) saliva, reported here, may be associated increased infectivity improved viral replicative fitness. also seven (ACTN1, H2AC2, GSN, NDKA, CD109, GGH, PCYOX) that yielded comprehension into infection performed outstandingly screening a hospital setting. This panel presented enhanced anti-COVID-19 anti-inflammatory signature, providing insights disease severity, supported comparisons other proteome data sets. signature provided valuable host's SARS-CoV-2 infection, shedding light on pathophysiology COVID-19, particularly cases mild disease. It underscores potential clinical applications saliva settings. Data are available via ProteomeXchange identifier PXD054133.

Language: Английский

Citations

0
Methylglyoxal Formation—Metabolic Routes and Consequences DOI Creative Commons
Janka Vašková,

Gabriela Kováčová,

Jakub Pudelský

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 212 - 212

Published: Feb. 13, 2025

Methylglyoxal (MGO), a by-product of glycolysis, plays significant role in cellular metabolism, particularly under stress conditions. However, MGO is potent glycotoxin, and its accumulation has been linked to the development several pathological conditions due oxidative stress, including diabetes mellitus neurodegenerative diseases. This paper focuses on biochemical mechanisms by which contributes through formation advanced glycation end products (AGEs), interactions with antioxidant systems, involvement chronic diseases like diabetes, neurodegeneration, cardiovascular disorders. exerts effects multiple signaling pathways, NF-κB, MAPK, Nrf2, induce stress. Additionally, triggers apoptosis primarily via intrinsic extrinsic while endoplasmic reticulum (ER) mediated PERK-eIF2α IRE1-JNK signaling. Moreover, activation inflammatory RAGE crucial pathogenesis these study points out connection between carbonyl increased formation, it should be an incentive search for marker that could have prognostic significance or targeted therapeutic intervention various

Language: Английский

Citations

0
A portrait of the infected cell: how SARS-CoV-2 infection reshapes cellular processes and pathways DOI Creative Commons

Valentina Marano,

Štěpánka Vlachová,

Sofia Maria Luigia Tiano

et al.

npj Viruses, Journal Year: 2024, Volume and Issue: 2(1)

Published: Dec. 17, 2024

Positive-sense single-stranded RNA (+ssRNA) viruses exert a profound influence on cellular organelles and metabolic pathway by usurping host processes to promote their replication. In this review, we present portrait of selected pathways perturbed in SARS-CoV-2 infection: the effect viral translation, replication assembly morphology function ER, remodelling degradative with focus autophagic processes, alterations affecting membranes lipid metabolism. For each these highlight specific factors involved interplay microscopic tug-of-war between pro-viral anti-viral effects that ultimately tip scale toward propagation or resolution infection.

Language: Английский

Citations

1