Exploring the interaction between endornavirus and Sclerotinia sclerotiorum : mechanisms of phytopathogenic fungal virulence and antivirus

mBio, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

ABSTRACT Hypovirulence-associated mycoviruses have the potential as biocontrol agents for plant fungal disease management, and exploration of interactions between these phytopathogenic fungi can provide opportunities to elucidate underlying mechanisms hypovirulence antiviruses. We previously found that Sclerotinia sclerotiorum endornavirus 3 (SsEV3), belonging genus Betaendornavirus within family Endornaviridae , confers on fungus but remains unclear. In this study, we SsEV3-infected strain produced fewer sclerotia, failed form infection cushions hosts, exhibited increased cell vacuolation, was more sensitive abiotic stresses. SsEV3 evoked transcriptional rewiring in S. affecting genes related virulence factors pathogenicity RNAi pathway An unknown biological function gene Sssnf1 downregulated following infection. Deletion impaired cushion formation decreased . Five key RNAi-related were significantly upregulated, deletion Ssdcl2 contributed accumulation. Additionally, identified a hypothetical protein encoded by Sshp1 directly interacts with RNA-dependent RNA polymerase (RdRp) domain SsEV3. Although mutants normal colony morphology, they showed higher accumulation reduced resistance reactive oxygen species, indicating similar genes, plays an antiviral role response may represent new antivirus factor. Therefore, examination interaction provides insights into fungi. IMPORTANCE emerged promising agents, studying their helps uncover pathogenesis defense. This study critical its hypovirulence-associated endornavirus, SsEV3, elucidating molecular mycovirus-induced changes not only impairs traits, including sclerotial production also triggers host responses involving typical interference pathways. New factors, such based established system endornavirus. These findings deepen our understanding fungus-mycovirus interactions, highlighting reducing facilitating development mycovirus-based control strategies.

Language: Английский

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Molecular Characterization of the First Partitivirus from a Causal Agent of Salvia miltiorrhiza Dry Rot

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(3), P. 179 - 179

Published: Feb. 27, 2024

Root rot as a result of Salvia miltiorrhiza is common root disease caused by Fusarium spp., which has become one the main diseases affecting production S. miltiorrhiza. Currently, several hypovirulence-related mycoviruses have been identified in many phytopathogenic fungi, including show potential biological controls. In this study, we report new mycovirus, oxysporum partitivirus 1 (FoPV1), isolated from F. strain FCR51, causal agent dry rot. The FoPV1 genome contains two double-stranded RNA segments (dsRNA1 and dsRNA2). size dsRNA1 1773 bp, it encodes putative RNA-dependent polymerase (RdRp). dsRNA2 1570 bp length, encoding capsid protein (CP). Multiple sequence alignments phylogenetic analyses based on amino acid sequences RdRp CP proteins indicated that appears to be member family Partitiviridae related members genus Gammapartitivirus. Pathogenicity assay showed confers hypervirulence its host, oxysporum. This first infecting mycovirus

Language: Английский

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Microbial Production of Biopesticides for Sustainable Agriculture

Sustainability, Journal Year: 2024, Volume and Issue: 16(17), P. 7496 - 7496

Published: Aug. 29, 2024

Food security is threatened by biotic stress on crops, e.g., from invasive pests, in the context of climate change. Pest control has traditionally been achieved using synthetic pesticides, yet pollution and persistence toxicity some pesticides are inducing food contamination and, turn, generating public concern. As a consequence, biopesticides increasingly used, notably for organic crops. For instance, microorganisms produce biopesticidal compounds such as secondary metabolites or growth-inhibitory enzymes. Most reviews this topic mainly focus describing microbial species their active compounds. However, there very few studies various process parameters. Here, we review both factors controlling physicochemical conditions scaling up biopesticide production. We present bacteria (e.g., Bacillus thuringiensis), algae, fungi, viruses biocontrol mechanisms applications commercial biopesticides. The benefits genetic engineering enhancing activity drawbacks commercialization also discussed.

Language: Английский

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Discovery of novel mycoviruses from fungi associated with mango leaf spots

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 26, 2025

Mango (Mangifera indica) is a commercially significant fruit crop cultivated globally. However, leaf spot diseases are common in mango orchards, which severely impact the yield. Mycoviruses hold promise as potential biocontrol agents. To investigate this possibility, fungi were isolated from lesions, resulting identification of six strains that contained double-stranded RNA (dsRNA). Through BLASTx analysis NCBI non-redundant database, 27 mycovirus-related contigs identified, corresponded to 10 distinct viruses grouped into 8 lineages: Alternaviridae, Chrysoviridae, Partitiviridae, Polymycoviridae, Orthototiviridae, Deltaflexiviridae, Narnaviridae, and Bunyaviricetes. Full genomic sequences these characterized confirmed be associated with their host fungi. The findings included novel mycoviruses, three previously unreported discovered new hosts, one virus strain. These results highlight diversity taxonomy mycoviruses found spots.

Language: Английский

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Colletotrichum associated partitivirus 1 infection negatively modulates the fungal growth of Colletotrichum zoysiae and its virulence to Zoysia matrella

International Turfgrass Society research journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Abstract Anthracnose, caused by Colletotrichum spp., is a severe and devastating disease in turfgrasses. While mycoviruses have been identified several isolates from turfgrasses, their effects on host fungal phenotypes remain unclear. This study investigated the impact of associated partitivirus 1 (CaPV1) infection zoysiae isolate MBCT‐264 Zoysia matrella . CaPV1‐infected virus‐free C. isogenic strains were established through five successive passages potato dextrose agar (PDA) with or without virus polymerase inhibitor. Comparative growth analysis PDA revealed slower strain compared to strain. Inoculation tests Z. leaf blades demonstrated that CaPV1‐free produced larger lesions more pronounced mycelium penetration than virus‐infected These findings suggest CaPV1 negatively affects both virulence , highlighting potential modulating turfgrass pathogen behavior.

Language: Английский

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Identification of a novel Gammapartitivirus associated with endophytic Fusarium oxysporum through transcriptome dataset mining

VirusDisease, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Language: Английский

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Two novel mitoviruses coinfecting the fungus Colletotrichum karstii

Archives of Virology, Journal Year: 2025, Volume and Issue: 170(5)

Published: April 11, 2025

Language: Английский

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Genomic characterization of a novel gammapartitivirus infecting the phytopathogenic fungus Colletotrichum cliviicola

Archives of Virology, Journal Year: 2025, Volume and Issue: 170(5)

Published: April 15, 2025

Language: Английский

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A Hypovirulence-Associated Partitivirus and Re-Examination of Horizontal Gene Transfer Between Partitiviruses and Cellular Organisms

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3853 - 3853

Published: April 18, 2025

Previous research has unearthed the integration of coat protein (CP) gene from alphapartitivirus into plant genomes. Nevertheless, prevalence this horizontal transfer (HGT) between partitiviruses and cellular organisms remains an enigma. In our investigation, we discovered a novel partitivirus, designated Sclerotinia sclerotiorum 1 (SsAPV1), hypovirulent strain sclerotiorum. Intriguingly, traced homologs SsAPV1 CP to genomes, including Helianthus annuus. To delve deeper, employed RNA-dependent RNA polymerase (RdRP) sequences as "bait" search NCBI database for similar sequences. Our unveiled widespread occurrence HGT viruses all five genera within family Partitiviridae other organisms. Notably, numerous CP-like RdRP-like genes were identified in genomes plants, protozoa, animals, fungi, even, first time, archaeon. The majority RdRP integrated insect respectively. Furthermore, detected DNA fragments originating genome some subcultures virus-infected strains. It suggested that may possesses reverse transcriptase activity, facilitating viral organism function requires further confirmation. study not only offers hypovirulence-associated partitivirus with implications fungal disease control but also sheds light on extensive events enhances comprehension origins, evolution, ecology partitiviruses, well evolution

Language: Английский

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Alternaria alternata botybirnavirus 1 (AaBRV1) Infection Affects the Biological Characteristics of Its Host Fungus Alternaria alternata

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(5), P. 376 - 376

Published: May 15, 2025

A botybirnavirus, Alternaria alternata botybirnavirus 1 (AaBRV1), had been identified from strain SD-BZF-19 isolated diseased watermelon leaves in our previous study. In the current study, AaBRV1 was eliminated its host fungus using single hyphal tip culture method combined with high-temperature treatment to obtain AaBRV1-free strain, which named SD-BZF-19-G14. Compared SD-BZF-19-G14, following infection, colony color of changed, and growth rate, dry weight mycelial biomass, sensitivity difenoconazole, fludioxonil, tebuconazole all decreased. However, virulence not significantly different disease index inoculated SD-BZF-19-G14 being 90.83 81.67, respectively. total 1244 differentially expressed genes (DEGs) were a comparative transcriptome analysis between two strains, Relative SD-BZF-19, number upregulated downregulated DEGs 660 584, Notably, Pfam database annotated that related Major Facilitator Superfamily (MFS) Cytochrome P450 (CYP450) 36 28, To knowledge, this is first documentation biological characteristics induced by infection A. alternata.

Language: Английский

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