Maize associated bacterial and fungal microbiomes show contrasting conformation patterns dependent on plant compartment and water availability

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 9, 2025

Plant-associated microorganisms can help crops to alleviate stress and increase the resilience of agricultural ecosystems climate change. However, we still lack knowledge on dynamics soil plant microbiomes their response changing conditions. This information is essential for development microbiome-based solutions improve crop stressors associated with In this work, explored: (i) conformation bacterial fungal assemblages different compartments (bulk soil, rhizosphere, roots, leaves grains) along cycle maize in an open field trial; (ii) effect water restriction microbiome, comparing optimal irrigation a 30% reduction supply. Our results show dynamic compartment-driven recruitment contrasting patterns bacteria fungi that were intensified towards end cycle. Roots showed most differentiated assemblage while conformed very distinct community leaves, suggesting relevant contribution aerial propagules microbiome organ. Regarding grain, communities looked closer those more like root. Despite reductions growth yield, limited-watered plants did not severe alterations. Still, significant impacts observed within compartments, being responsive limited watering than bacteria, hallmark ASVs each compartment regime. Network analysis suggests may play roles shifts under limitation. study highlights importance conducting multikingdom analyses holistic understanding evolution microbial whole environmental stressors.

Language: Английский

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Effects of plant tissue permeability on invasion and population bottlenecks of a phytopathogen

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 2, 2024

Abstract Pathogen genetic diversity varies in response to environmental changes. However, it remains unclear whether plant barriers invasion could be considered a bottleneck for phytopathogen populations. Here, we implement barcoding approach generate pool of 90 isogenic and individually barcoded Ralstonia solanacearum strains. We used these strains inoculate tomato plants with different degrees physical permeability (intact roots, wounded roots xylem inoculation) quantify the population dynamics during invasion. Our results reveal that impacts degree bottleneck, diversity, composition also find selection is main driver structuring pathogen populations when infection are less permeable, i.e., intact removal root immune predominance stochasticity assembly. Taken together, our study suggests constitutes diversity.

Language: Английский

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How does particulate matter affect plant transcriptome and microbiome?

Environmental and Experimental Botany, Journal Year: 2023, Volume and Issue: 209, P. 105313 - 105313

Published: March 27, 2023

Phylloremediation for the reduction of air particulate matter (PM) is an interesting opportunity to significantly contribute improve quality urban environment. The aim this study was to: 1) gain insight into gene regulatory networks modulating leaf responses polluted air, 2) identify possible changes in microbiome due real transcriptome and were analyzed Photinia x fraseri L. plants cultivated three months pots two close-by areas under different levels PMs (low high). PCA heat map analysis showed that 28 differentially expressed genes common between pairwise comparisons able clearly discriminate higher PM levels. pollutants mainly sensed by through a restructuring modification cell wall membrane main repression lipid desaturases. In addition, high clear belonging primary metabolism pathways involved C assimilation. Microbiome no significant taxonomic diversity indexes bacterial communities, whereas fungi genera Epicoccum Dioszegia differently affected exposure A model transcriptional regulation has been proposed.

Language: Английский

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Central Taxa Are Keystone Microbes During Early Succession

Ecology Letters, Journal Year: 2024, Volume and Issue: 28(1)

Published: Dec. 31, 2024

Microorganisms underpin numerous ecosystem processes and support biodiversity globally. Yet, we understand surprisingly little about what structures environmental microbiomes, including how to efficiently identify key players. Microbiome network theory predicts that highly connected hubs act as keystones, but this has never been empirically tested in nature. Combining culturing, sequencing, networks field experiments, isolated 'central' (highly connected, hub taxa), 'intermediate' (moderately connected), 'peripheral' (weakly/unconnected) microbes experimentally evaluated their effects on soil microbiome assembly during early succession Central colonisers significantly (1) enhanced (35%-40% richer communities), (2) reshaped trajectories of (3) increased recruitment additional influential by > 60%. In contrast, peripheral did not increase diversity were transient taxa, minimally affected the presence other microbes. This work elucidates fundamental principles microbial ecology demonstrates for first time nature central keystone taxa.

Language: Английский

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The genetic and physiological basis of Arabidopsis thaliana tolerance to Pseudomonas viridiflava

New Phytologist, Journal Year: 2023, Volume and Issue: 240(5), P. 1961 - 1975

Published: Sept. 4, 2023

The opportunistic pathogen Pseudomonas viridiflava colonizes > 50 agricultural crop species and is the most common in phyllosphere of European Arabidopsis thaliana populations. Belonging to P. syringae complex, it genetically phenotypically distinct from well-characterized sensu stricto. Despite its prevalence, we lack knowledge how A. responds native isolates at molecular level. Here, characterize host response an - pathosystem. We measured growth axenic infections used immune mutants, transcriptomics, metabolomics determine defense pathways influencing susceptibility infection. Infection with increased jasmonic acid (JA) levels expression ethylene pathway marker genes. a susceptible accession was delayed compared tolerant one. Mechanical injury rescued susceptibility, consistent involvement JA. JA/ethylene important for suppression viridiflava, yet capacity varies between accessions. Our results shed light on can suppress ever-present but further studies are needed understand evades this spread broadly across

Language: Английский

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Microbial communities living inside plant leaves or on the leaf surface are differently shaped by environmental cues

ISME Communications, Journal Year: 2024, Volume and Issue: 4(1)

Published: Jan. 1, 2024

Abstract Leaf-associated microbial communities can promote plant health and resistance to biotic abiotic stresses. However, the importance of environmental cues in assembly leaf endo- epi-microbiota remains elusive. Here, we aimed investigate impact seasonal variations, on establishment microbiome, focusing long-term changes (five years) bacterial, fungal, nonfungal eukaryotic colonizing surface endosphere six wild Arabidopsis thaliana populations. While leaf-microbial were found be highly stochastic, niche had a predominant with endophytic consistently exhibiting lower diversity variability. Among factors, radiation- humidity-related factors are most important drivers patterns leaf, stronger effects epiphytic communities. Using linear models, identified 30 genera whose relative abundance compartments could modeled from variables, suggesting specific preferences for these taxa. With hypothesis that interactions within communities, analyzed interaction networks across compartments. We showed more complex than complexity connectivity partially correlated mentioned cues. Our results indicate humidity solar radiation function as major shaping phyllosphere microbiome at both micro (leaf compartment) macro (site) scales. These findings have practical implications predicting developing field-adapted microbes face global change.

Language: Английский

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Bacterial community composition of wheat aboveground compartments correlates with yield during the reproductive phase

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

Plant-associated microbial communities play important roles in agricultural productivity, and their composition has been shown to vary across plant compartments developmental stages. However, the response of within different at stages diverse long-term fertilization treatments, as well linkages with crop yields, remains underexplored. This study analyzed wheat-associated bacterial various soil under three treatments throughout vegetative reproductive phases. The variance community was primarily attributed compartments, followed by belowground (bulk soil, rhizosphere root) exhibited stronger responses than aboveground (stem leaf). responded all stages, it significantly correlated yields during phase, whereas only showed a which point yields. Moreover, this co-occurrence network enhanced complexity, contained an increased number keystone species associated such

Language: Английский

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Proteins released into the plant apoplast by the obligate parasitic protist Albugo selectively repress phyllosphere-associated bacteria

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: May 17, 2022

Biotic and abiotic interactions shape natural microbial communities. The mechanisms behind microbe-microbe interactions, particularly those protein-based, are not well understood. We hypothesize that secreted proteins a powerful highly specific toolset to defend plant niches. have studied Albugo candida , an obligate parasite from the protist Oomycota phylum, for its potential modulate growth of bacteria through secretion into apoplast. Amplicon sequencing network analysis -infected uninfected samples revealed abundance negative correlations between other phyllosphere microbes. Analysis apoplastic proteome colonized leaves combined with machine-learning predictors enabled selection candidates heterologous expression study their inhibitory activity. found three candidate show selective antimicrobial activity on gram-positive isolated Arabidopsis thaliana these inhibited important stability community structure. could ascribe antibacterial intrinsically disordered regions positively correlate it net charge. This is first report under conditions therefore biocontrol tools targeted manipulations microbiome.

Language: Английский

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Compartments of roots and mature leaves are key hubs in the connectivity of tea-plant mycobiomes and are influenced by environmental factors and host age

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 893, P. 164827 - 164827

Published: June 15, 2023

Understanding the tripartite consortium of crop, mycobiome, and environment is necessary to advance smart farming. Owing their life cycle hundreds years, tea plants are excellent models for studying these entwined relationships; however, observations on this globally important cash crop with numerous health benefits still rudimentary. Here, fungal taxa along soil-tea plant continuum in gardens different ages famous high-quality tea-growing regions China were characterized using DNA metabarcoding. Using machine learning, we dissected spatiotemporal distribution, co-occurrence patterns, assembly, associations compartments tea-plant mycobiomes, further explored how potential interactions driven by environmental factors tree age, they influenced market prices tea. The results revealed that Compartment niche differentiation was key driving force behind variation mycobiome. mycobiome roots had highest specific proportion convergence almost did not overlap soil. enrichment ratio developing leaves root increased increasing while mature showed value Laobanzhang (LBZ) garden top displayed strongest depletion effect association continuum. balance between determinism stochasticity assembly process co-driven compartment niches variation. Fungal guild analysis altitude indirectly affected mediating abundance pathogen. relative importance pathogen ectomycorrhizae could be used assess age Biomarkers mainly distributed soil compartments, Clavulinopsis miyabeana, Mortierella longata, Saitozyma sp. may affect dynamics mycobiomes ecosystem services. Soil properties (mainly total potassium) via positively influencing leaves. In contrast, climate directly significantly drove composition Moreover, negative correlations network regulated which structural equation model complexity as hub. These findings indicate signatures play pivotal roles adaptive evolution disease control can help develop better agricultural practices focus both financial profits, provide a new strategy assessing quality grade age.

Language: Английский

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Ecotypic adaptation of plants and the role of microbiota in ameliorating the environmental extremes using contemporary approaches

Elsevier eBooks, Journal Year: 2023, Volume and Issue: unknown, P. 377 - 402

Published: Jan. 1, 2023

Language: Английский

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