Organ-specific microbiomes in naturalLotus corniculatuspopulations: Metacommunity dynamics in the plant endosphere

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

ABSTRACT The structure of plant microbial communities vary due to a broad range factors such as host and environmental factors, abiotic biotic perturbations, various assembly processes occurring at multiple tempo-spatial scales. In natural environments are constantly exposed perturbations processes. Thus, attain systemic understanding the ecology microbiomes, it is essential study that influence patterns community structures in environments. this we examined bacterial, fungal, eukaryotic organs Lotus corniculatus populations seven grassland sites for four years. We used framework metacommunity theory understand shape variations by defining associated with roots, shoots, flowers, seeds distinct linked dispersal. show organ-specificity endophytic communities. Our findings suggest selective filtering organs, interactions, well scales result core microbiomes organs. addition, transmission microorganisms from within outside hosts accounts yet overlapping organ microbiomes. could provide comprehensive knowledge stochastic deterministic conditions. Understanding these ecological harnessing beneficial effects plant-associated on productivity, resilience, pathogen defense.

Language: Английский

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Prospects for deploying microbes against tree-killing beetles (Coleoptera) in Anthropocene

Frontiers in Forests and Global Change, Journal Year: 2023, Volume and Issue: 6

Published: July 26, 2023

Forests form rich biodiversity hubs that act as large reservoirs of natural carbon. The spatial and temporal heterogeneity these complex habitats forest floors provides ecological services immense socio-economic importance. However, hotspots are incessantly exposed to multifarious abiotic, biotic, anthropogenic disturbances, amongst which unpredictable pest (i.e., bark beetle) outbreak account for the loss vegetation microbiome measurable quantum. importance in forming an inseparable functional unit every host shaping its interaction with other partners has been well realized. Interestingly, pests, including beetles, also reported rely on their endosymbiotic microbial manipulate tree defense machinery. In contrast, holobiont trees regulates overall function fitness significantly contributes tackling challenging situations. Nevertheless, how directly or indirectly influence beetle is still enigma. present review shall elaborate role tools enhancing performance fitness, helps counter damage. Besides, it emphasize exploiting microorganisms acting biocontrol agents shielding against destruction. application endosymbiont-mediated RNA interference (RNAi) working two-tier specificity controlling devastations be discussed new-age technological advances. All explanations expected put forth potential toolbox offering better more sustainable management strategies future.

Language: Английский

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Bacillus subtilis 26D Triggers Induced Systemic Resistance against Rhopalosiphum padi L. by Regulating the Expression of Genes AGO, DCL and microRNA in Bread Spring Wheat

Microorganisms, Journal Year: 2023, Volume and Issue: 11(12), P. 2983 - 2983

Published: Dec. 14, 2023

Bacillus subtilis 26D is a plant growth-promoting endophytic bacteria capable of inducing systemic resistance through the priming mechanism, which includes genome reprogramming and phenomenon RNA interference (RNAi) microRNA (miRNAs). The phloem-feeding insect bird cherry-oat aphid Rhopalosiphum padi L. serious pest that causes significant damage to crops throughout world. However, function miRNAs in response infestation remains unclear. results this work showed B. stimulated wheat plants, expression genes hormonal signaling pathways ICS, WRKY13, PR1, ACS, EIN3, PR3, ABI5. In addition, activated RNAi mechanism regulated nine conserved activation ethylene, salicylic acid (SA), abscisic (ABA) pathways, was demonstrated by using treatments with phytohormones. Treatment plants SA, ABA acted similar manner on induction AGO4, AGO5 DCL2, DCL4 genes, as well miRNAs. Different patterns miRNA were found aphid-infested treated or infested aphids, suggesting play multiple roles insects, associated effects redox metabolism, synthesis secondary metabolites. Our study provides new data further elucidate fine mechanisms bacterial-induced priming. extensive needed fully unravel these mechanisms.

Language: Английский

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Life-stage and geographic location determine the microbial assemblage in Eurasian spruce bark beetle, Ips typographus L. (Coleoptera: Curculionidae)

Frontiers in Forests and Global Change, Journal Year: 2023, Volume and Issue: 6

Published: July 13, 2023

Ips typographus L. (Coleoptera: Curculionidae) is one of the serious pests causing mass destruction European spruce forests, with a substantial economic impact. Symbiotic microbes associated bark beetles often play definitive role in accomplishing their physiological and ecological functions by detoxifying chemicals, inhibiting pathogens, offering nutrients. Although few research works explored I. , much yet to be studied understand adaptive ecology as holobionts comprehensively. The present study examined bacterial fungal communities larvae, adults, feeding galleries from Austria Czech Republic using high-throughput sequencing that elucidated influence geographic location, host, life stage on microbial assemblage Eurasian beetle, . most abundant genera included Pseudoxanthomonas Spiroplasma Pseudomonas Cellulomonas Tyzzerella Bacillus Mycobacterium Alternatively, Wickerhamomyces Nakazawaea Aspergillus Ophiostoma Cryptococcus Rhexographium Yamadazyma Talaromyces Kuraishia were highly dominant genera. Significant differences community richness diversity detected among tested samples. LEfSe analysis revealed species-specific biomarkers different locations gallery PICRUSt2 FUNGuild documented putative roles beetle holobiont provided foundation for downstream functional analyses. current findings further enhanced our understanding holobionts.

Language: Английский

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Comparative metagenomic study unveils new insights on bacterial communities in two pine-feeding Ips beetles (Coleoptera: Curculionidae: Scolytinae)

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 9, 2024

Background Climate change has recently boosted the severity and frequency of pine bark beetle attacks. The bacterial community associated with these beetles acts as “hidden players,” enhancing their ability to infest thrive on defense-rich trees. There is limited understanding environmental acquisition hidden players life stage-specific association different pine-feeding beetles. inadequate knowledge novel introduction trees after infestation. Hence, we conducted first comparative metabarcoding study revealing communities in before feeding stages two dominant beetles, namely Ips sexdentatus acuminatus . We also evaluated between wild lab-bred measure deviation due inhabiting a controlled environment. Results Significant differences amplicon sequence variance (ASVs) abundance existed among within However, Pseudomonas, Serratia, Pseudoxanthomonas, Taibaiella, Acinetobacter served core bacteria. Interestingly, I. larvae correspond significantly higher diversity richness evenness compared other developmental stages, while adults displayed no significant variation stages. Both showed prevalence family Pseudomonadaceae. In addition, dominance Yersiniaceae, whereas Erwiniaceae was abundant Alternatively, Acidobacteriaceae , Corynebacteriaceae Microbacteriaceae were highly families lab-bred, Chitinophagaceae accuminatus. validated relative abundances selected taxa estimated by metagenomic sequencing quantitative PCR. Conclusion Our sheds new insights into associations under influence various drivers such environment, host, documented that lab-breeding considerably influences assembly. Furthermore, alters bacteriome at microhabitat level. Nevertheless, our revisited symbiosis revealed intriguing insight assembly, facilitating future functional studies.

Language: Английский

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Advancements in double-stranded RNA (dsRNA) technology for effective management of forest insect pests

Southern Forests a Journal of Forest Science, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 19

Published: Dec. 11, 2024

AbstractThe emergence of forest insects as a result climatic changes presents traditional management strategies with significant challenges. RNA interference (RNAi) has attracted attention possible innovative conservation strategy. By eliciting gene silencing double-stranded (dsRNA), RNAi can effectively control insect pests, especially those belonging to the order Coleoptera. However, implementation in pest is hindered by varying efficacy, target selection, dsRNA design and delivery techniques, particularly for long-lived endophagous such bark beetles Asian long-horned beetles. This review examines development pest-combating systems nanoparticles, symbiont-mediated, cationic liposome-assisted plant-mediated delivery. The study highlights need risk assessments protection products (FPPs) using suggests integrating off-target prediction capabilities based on sequence complementarity analysis. Despite obstacles, shows promise controlling wood-boring beetles, additional research could boost its effectiveness against these insects.Keywords: Coleopteraforest productsRNAi deliveryRNA (RNAi)wood-boring

Language: Английский

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Phenotypic plasticity plays an essential role in the confrontation between plants and herbivorous insects

CABI Agriculture and Bioscience, Journal Year: 2023, Volume and Issue: 4(1)

Published: Dec. 13, 2023

Abstract The interaction between insects and plants is a classic case of coevolution. During the arms race that has continued for 400 million years, mutualistic (such as pollination defense assistance) antagonistic relationships gradually formed complicated under selection pressure from phytophagous insects. Thus, have developed diverse strategies, constantly balancing relationship growth. At same time, evolved ability to adapt resist plant defenses. Throughout this process, phenotypic plasticity continuously helped both groups new environments niches. Epigenetic changes play an important role in formation plastic phenotype. These allow parental traits be passed on offspring, helping offspring insect feeding. genetic variation provide basis phenotypes, can form stable long-term feeding pressure. In review, we summarize strategies counter-defense insects, suggest plays interaction, discuss epigenetics phenotypes.

Language: Английский

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Comparative metagenomic study unveils bacterial communities and their putative involvement in ecological success of two pine-feedingIpsbeetle holobionts

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 24, 2024

Abstract Climate change has recently boosted the severity and frequency of pine bark beetle attacks. The bacterial community associated with these beetles acts as “hidden players”, enhancing their ability to infest thrive on defence-rich trees. There is limited understanding environmental acquisition hidden players life stage-specific association different pine-feeding beetles. inadequate knowledge novel introduction trees after infestation. Hence, we conducted first comparative metabarcoding study comprehensively revealing communities in before feeding stages two dominant beetles, namely Ips sexdentatus acuminatus . We also evaluated between wild lab-bred measure deviation due inhabiting a controlled environment. Significant differences amplicon sequence variance (ASVs) abundance existed among within Such observations endorsed that stage shaped assemblage. Furthermore, wild-collected adult had distinct assemblages, implying breeding environment induced crucial changes. Alteration wood bacteriome an intriguing observation present study, which demands further investigation. validated relative abundances selected taxa estimated by metagenomic sequencing quantitative PCR. Functional predictions revealed genera might execute conserved functions, aiding ecological success Nevertheless, findings shed new insights into associations putative metabolic roles under influence various drivers such environment, host, provide foundation for future downstream functional investigations. Importance current holobiont restricted. Most studies lack information microbial assembly microhabitats. No data reveals lab associations. It unknown if there any adaptive convergence assemblage same host. essential developing management strategy restore forests from beetle-mediated damage. Our shows lab-breeding considerably influences assembly. documented alters at microhabitat level, shapes our revisited symbiosis insight assembly, facilitating studies.

Language: Английский

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Analysis of differential effects of host plants on the gut microbes of Rhoptroceros cyatheae

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: June 19, 2024

As an indispensable part of insects, intestinal symbiotic bacteria play a vital role in the growth and development insects their adaptability. Rhoptroceros cyatheae , main pest relict plant Alsophila spinulosa poses serious threat to A. population. In present study, 16S rDNA internal transcribed spacer high-throughput sequencing techniques were used analyze structure microbes diversity insect feeding on two different plants, as well similarities between microorganisms R. . The dominant leaf endophytes also compared based data. results showed that Proteobacteria, Firmicutes, Actinobacteria phyla bacteria, Ascomycota was phylum fungi. Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium Methylobacterium-Methylorubrum Enterococcus genera intestine R relative abundance significantly groups. Candida common genus fungi groups, no significant difference observed its This with greatly affected by food. core microbiota leaves gut indicated presence microbial exchange two. network correlation diagram Gymnosphaera metteniana more closely related each other, which could help host better cope adverse external environment. study provides theoretical basis for adaptation mechanism new direction effective prevention control

Language: Английский

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Diffusive Phyllosphere Microbiome Potentially Regulates Harm and Defence Interactions Between Stephanitis nashi and Its Crabapple Host

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 23, 2024

ABSTRACT Pear lace bug ( Stephanitis nashi ) is a significant herbivorous pest, harbouring diverse microbiome crucial for crabapple Malus sp.) host adaptation. However, the mutual influence of S. ‐ and plant‐associated microbiomes on plant responses to pest damage remains unclear. This study found that significantly altered bacterial community structure reduced evenness in phyllosphere. Notably, diversity within was lower than environment, potentially influenced by insect developmental stage, diffusion stage endosymbiont species number abundance. Extensive correlation effect between adjacent environments were observed, evident gradual decrease an increase acquisition ratio from soil phyllosphere . Correspondingly, impacted metabolic response leaves, altering pathways involved vitamin, amino acid lipid metabolism so forth. Furthermore, association analysis linked these changes alterations, emphasizing important role diffusive regulating ‐crabapple interactions. highlights plants their potential adaptability defence responses, providing new insights into plant−insect−microbiome

Language: Английский

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