Microorganisms for sustainability, Journal Year: 2024, Volume and Issue: unknown, P. 155 - 177
Published: Jan. 1, 2024
Language: Английский
Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: 136, P. 102565 - 102565
Published: Jan. 5, 2025
Language: Английский
Citations
0
Plants, Journal Year: 2025, Volume and Issue: 14(3), P. 355 - 355
Published: Jan. 24, 2025
Abscisic acid (ABA) is not only important for plant responses to abiotic stresses, but also plays a key and multifaceted role in immunity. In this work, we analyzed the of ABA development resistance/susceptibility wheat (Triticum aestivum L.)–Stagonospora nodorum Berk. pathosystem, which includes recognition necrotic effectors (NEs) pathogen by corresponding susceptibility genes. We studied interaction S. SnB isolate, produces two NEs, SnToxA SnTox3, with three genotypes having different combinations host genes (Tsn1 Snn3-B1). The results work on gene expression redox status resistant sensitive treated show that signaling directed at inducing resistance plants isolate through activation early post-invasive defense TaERD15 TaABI5. induction these leads reactive oxygen species (ROS) accumulation during stage infection, subsequent limitation pathogen’s growth. presence compatible SnTox3–Snn3-B1, suppressed. On contrary, SnToxA–Tsn1, activated, activity TaABI5 inhibited, NAC (NAM, ATAF1/2, CUC2) transcription factor (TF) family TaNAC29 TaNAC21/22 induced. TF induce SnB, associated decrease oxidative burst infection. Thus, our study provides new data NEs SnTox3 manipulating nodorum. Deepening knowledge area will be instrumental developing strategies breeding programs contribute environmentally friendly sustainable agriculture.
Language: Английский
Citations
0
IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 27, 2025
Bacillus subtills is a Gram-positive, rod-shaped, aerobic, non-pathogenic bacterium that synthesizes resilient endospores. Due to the capability of forming spores, organism has ecological versatility allows thrive in distinct environments such as soil, rhizospheric zones plants, and even gastrointestinal tracts animals. It widely known plant growth-promoting rhizobacterium (PGPR) enhances plants’ growth by producing various primary secondary metabolites phytohormones, volatile organic compounds (VOC), enzymes (exo), biosurfactants, siderophores, solubilizing phosphate, fixing nitrogen. The bacterium’s utility agriculture further supported formulation delivery methods, enhancing its effectiveness biopesticide biofertilizer. Additionally, B. subtilis contributes soil health sustainability protective biofilms generating antimicrobial compounds, which suppress progression deleterious microorganisms. Metabolites also degrade remediates heavy metals hydrocarbons aids maintaining environment. Despite wide-ranging values, challenges are still there terms usage optimization. Future perspectives highly required would enhance all beneficial traits integrate into integrated pest management (IPM) systems potential pollutant remediator promote sustainable agricultural environmental practices, documented ‑chapter.
Language: Английский
Citations
0
Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16
Published: March 20, 2025
Introduction Cotton ( Gossypium hirsutum L.) plays a vital role in Pakistan’s economy, providing significant employment opportunities and supporting the country’s textile industry. However, cotton productivity is severely impacted by pests diseases, such as black spots caused sooty mold, posing critical challenges to sustainable agriculture. This study investigates novel integration of plant growth-promoting rhizobacteria (PGPR) with recommended NPK fertilizers micronutrients enhance growth, yield, disease resistance, post-harvest soil properties. Methodology A consortium Bacillus megaterium (ZR19), Paenibacillus polymyxa (IA7), sp. (IA16) were evaluated under six treatments: control (T1), PGPR (T2), (T3), + (T4), (T5), (T6). Results The results depicted increase antioxidant activities 19% superoxide dismutase (SOD), 29% peroxidase (POX), 28% (POD), 14% catalase (CAT) activity T6 compared control. Similarly, growth parameters substantially improved root length (39%), shoot (19%), biomass up 31 20%, respectively, T6. Moreover, yield attributes like single boll weight lint percentage also enhanced 32 13%, integration. In contrast, demonstrated considerable biocontrol potential against incidence was reduced 68% cotton, index 75%, efficacy reached 75%. biological chemical properties, including bacterial populations, microbial nitrogen, organic matter, essential nutrient availability. Discussion So, these findings witnessed dual behavior strains balanced nutrition can lead us development an effective biopesticide cum biofertilizer for production arid conditions combating mold effectively.
Language: Английский
Citations
0
Plant Pathology, Journal Year: 2025, Volume and Issue: unknown
Published: April 23, 2025
ABSTRACT This research aimed to examine the co‐culture of two Bacillus species in order enhance damping‐off disease control kale plants. The microbial vallismortis strain TU‐Orga21 and B. subtilis TU‐Orga1 significantly inhibited Pythium aphanidermatum DOA compared single strains, reducing mycelium growth by up 39.26% causing deformations hyphal structures. Synchrotron radiation‐based Fourier‐transform infrared (SR‐FTIR) microspectroscopy was used investigate biochemical changes P. mycelia showed lowest polysaccharide content (cellulose β‐glucans) after being treated with co‐culture. In addition, use matrix‐assisted laser desorption ionisation dual time‐of‐flight tandem mass spectrometry (MALDI‐TOF/TOF MS) high‐performance liquid chromatography (HPLC) evaluate lipopeptides TU‐Orga21, their revealed that interactions between microorganisms resulted an approximately 350% increase production iturin A, which has plant pathogen‐inhibiting ability. Additionally, both strains produce surfactin, quantifiable levels detected all treatments, further supporting potential as a biocontrol consortium. A greenhouse study confirmed treating plants co‐culture, applied twice before inoculation, effectively controlled damping‐off, achieving results equal metalaxyl treatment, 100% reduction incidence, more effective than using either alone. Moreover, application promoted greater canopy width height positive negative controls. Together, these findings indicate beneficial bacteria can be anti‐oomycete promote for enhanced biological control.
Language: Английский
Citations
0
Agronomy, Journal Year: 2024, Volume and Issue: 14(4), P. 771 - 771
Published: April 9, 2024
Brown rot disease caused by Monilinia fructicola is one of the most important peach fruit threats in world. The use biological control agents (BCAs), instead synthetic fungicides, to successfully inhibit postharvest development a challenge sustainable and efficient crop management. commercially available BCA Bacillus amyloliquefaciens QST 713 (formerly subtilis QST713) able variety fungal pathogens suppress several plant diseases. Our results showed that this inhibited mycelial growth vitro, was disease’s severity fruits via delaying reducing brown symptoms. A transcriptomic analysis during their pre-treatment with biocontrol agent following M. revealed significant upregulation specific differentially expressed genes (DEGs) at 48 h after inoculation (HAI). These are related activation transcriptional factors, such as members WRKY NAC families, receptors involved pathogen recognition signaling transduction (e.g., LRR-RLKs). Furthermore, inhibition confirmed analyzing expression profiles genes, which highlighted direct antimicrobial impact bacterial strain against fungus. Hence, these findings clearly suggest B. an disease, can directly improve tolerance.
Language: Английский
Citations
3
Plants, Journal Year: 2024, Volume and Issue: 13(18), P. 2546 - 2546
Published: Sept. 10, 2024
The SnTox1 effector is a virulence factor of the fungal pathogen Stagonospora nodorum (Berk.), which interacts with host susceptibility gene Snn1 in gene-for-gene manner and causes necrosis on leaves sensitive wheat genotypes. It known that salicylic acid (SA), jasmonic (JA) ethylene are key phytohormones involved plant immunity. To date, effectors various pathogens have been discovered can manipulate hormonal pathways even use hormone crosstalk to promote disease development. However, role manipulating has not studied detail. We redox status expression twelve genes two MAPK six bread cultivars insensitive or without treatment by SA, JA ethephon (ethylene-releasing agent) during infection SnTox1-producing isolate S. 1SP. results showed controls antagonism between SA JA/ethylene signaling pathways. pathway was development susceptibility, were plants resistance Sn1SP presence SnTox1-Snn1 interaction. hijacked suppress catalase activity, increase hydrogen peroxide content induce formation; it simultaneously suppresses SA. do this, reprogrammed TaMRK3 TaMRK6 TF TaWRKY13, TaEIN3 TaWRKY53b. This study provides new data pathosystem nodorum.
Language: Английский
Citations
2
Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15
Published: Oct. 9, 2024
Walnut ( Juglans spp.), a significant deciduous tree of economic and ecological importance, faces substantial threats from walnut anthracnose, primarily caused by Colletotrichum gloeosporioides . Bacillus velezensis has shown promise in mitigating this fungal pathogen. To delve deeper into the induction mechanism B. on plant resistance, we conducted metabolomic analysis leaves six different treatment groups. Specifically, groups were defined as follows: Group B.v. was inoculated with alone, CK served blank control, C.g. solely C. B.v.−C.g. received followed inoculation. B.v.+C.g. underwent simultaneous inoculation both , while C.g.−B.v. treated first then A total 1,503 metabolites detected, mainly including flavonoids, terpenoids, steroids. The results revealed that spraying not only enhanced inherent resistance plants but also significantly regulated already infected This achieved inducing to adjust their metabolic pathways such salicylic acid, jasmonic abscisic thereby strengthening stress response. Transcriptomic correlation analyses showed comparisons vs. CK, C.g., 59, 244, 122 differential abundance along 7860, 3677, 5587 genes, respectively. Amino acid synthesis, starch sucrose metabolism, photosynthesis, phenylpropane purine glutathione metabolism played crucial roles walnut’s disease mechanism. Further induced regulate multiple LOC109005403 LOC108985444 LOC118344177 resulting production defensive palmitic coumarin ferulic enhancing In summary, induces systemic modulating acid. It enhances cell walls, synthesizing secondary metabolites, regulating energy responses. These findings provide solid theoretical foundation for future field application controlling anthracnose.
Language: Английский
Citations
0
Phytopathology Research, Journal Year: 2024, Volume and Issue: 6(1)
Published: Oct. 22, 2024
Abstract In plants, recognition between resistance gene ( R ) and virus induces a local hypersensitive response (HR), which is accompanied by systemic acquired (SAR). The dominant N in tobacco confers to mosaic (TMV) at both locally inoculated tissues systemically infected tissues. However, the mechanisms underlying HR- SAR-mediated viral inhibition are not fully revealed. this study, we find that Nicotiana glutinosa RNA-binding protein (NgRBP) an RNA silencing suppressor enhances TMV-triggered HR. Stronger HR could result stronger as well SAR. Enhanced leaves induced NgRBP compromised transient expression of NahG . These results indicate activated plays crucial role resistance. Our reveal crosstalk gene-mediated would deepen our understanding established SAR models.
Language: Английский
Citations
0
Metabolites, Journal Year: 2024, Volume and Issue: 14(12), P. 646 - 646
Published: Nov. 21, 2024
Background/Objectives: Plant growth-promoting rhizobacteria (PGPR), particularly Bacillus spp., are pivotal in enhancing plant defense mechanisms against pathogens. This study aims to investigate the metabolic reprogramming of pine needles induced by csuftcsp75 response pathogen Diplodia pinea P9, evaluating its potential as a sustainable biocontrol agent. Methods: Using liquid chromatography–mass spectrometry (LC-MS/MS), we performed principal component analysis and cluster assess alterations treated versus control groups. focused on specific metabolites associated with defense. Results: Our findings indicate that treatment significantly modifies profiles needles, leading notable increases flavonoid biosynthesis, phenylpropanoid metabolism, well amino acid metabolism pathways. These changes enhanced systemic acquired resistance (SAR) (ISR), plants exhibiting elevated levels defense-related compounds such 5-hydroxytryptophol oleanolic acid. Conclusions: reveals enhances P9 modulating needle activating key immune pathways, inducing resistance, offering natural alternative chemical pesticides agriculture.
Language: Английский
Citations
0