Journal of Fungi,
Journal Year:
2023,
Volume and Issue:
9(3), P. 362 - 362
Published: March 15, 2023
Fungi
represent
an
important
source
of
bioactive
secondary
metabolites
(SMs),
which
have
wide
applications
in
many
fields,
including
medicine,
agriculture,
human
health,
and
other
industries.
The
genes
involved
SM
biosynthesis
are
usually
clustered
adjacent
to
each
into
a
region
known
as
biosynthetic
gene
cluster
(BGC).
recent
advent
diversity
genetic
genomic
technologies
has
facilitated
the
identification
cryptic
or
uncharacterized
BGCs
their
associated
SMs.
However,
there
still
challenges
that
hamper
broader
exploration
industrially
metabolites.
advanced
CRISPR/Cas
system
revolutionized
fungal
engineering
enabled
discovery
novel
compounds.
In
this
review,
we
firstly
introduce
relationships
with
SMs,
followed
by
brief
summary
conventional
strategies
for
engineering.
Next,
range
state-of-the-art
CRISPR/Cas-based
tools
been
developed
review
these
methods
fungi
research
on
Finally,
limitations
systems
discussed
directions
future
proposed
order
expand
improve
efficiency
Journal of Fungi,
Journal Year:
2021,
Volume and Issue:
7(8), P. 606 - 606
Published: July 27, 2021
Aflatoxins
(AFs)
are
highly
toxic
and
cancer-causing
compounds,
predominantly
synthesized
by
the
Aspergillus
species.
AFs
biosynthesis
is
a
lengthy
process
that
requires
as
minimum
30
genes
grouped
inside
75
kilobytes
(kB)
of
gene
clusters,
which
regulated
specific
transcription
factors,
including
aflR,
aflS,
some
general
factors.
This
paper
summarizes
status
research
on
characterizing
structural
regulatory
associated
with
AF
production
their
roles
in
aflatoxigenic
fungi,
particularly
flavus
A.
parasiticus,
enhances
current
understanding
adversely
affect
humans
animals
great
emphasis
toxicity
preventive
methods.
Biotechnology Advances,
Journal Year:
2021,
Volume and Issue:
54, P. 107866 - 107866
Published: Nov. 12, 2021
Natural
products
from
fungi
represent
an
important
source
of
biologically
active
metabolites
notably
for
therapeutic
agent
development.
Genome
sequencing
revealed
that
the
number
biosynthetic
gene
clusters
(BGCs)
in
is
much
larger
than
expected.
Unfortunately,
most
them
are
silent
or
barely
expressed
under
laboratory
culture
conditions.
Moreover,
many
nature
uncultivable
cannot
be
genetically
manipulated,
restricting
extraction
and
identification
bioactive
these
species.
Rapid
exploration
tremendous
cryptic
fungal
BGCs
necessitates
development
heterologous
expression
platforms,
which
will
facilitate
efficient
production
natural
cell
factories.
Host
selection,
BGC
assembly
methods,
promoters
used
expression,
metabolic
engineering
strategies
compartmentalization
pathways
key
aspects
consideration
to
develop
such
a
microbial
platform.
In
present
review,
we
summarize
current
progress
on
above
challenges
promote
research
effort
relevant
fields.
Journal of Fungi,
Journal Year:
2023,
Volume and Issue:
9(3), P. 362 - 362
Published: March 15, 2023
Fungi
represent
an
important
source
of
bioactive
secondary
metabolites
(SMs),
which
have
wide
applications
in
many
fields,
including
medicine,
agriculture,
human
health,
and
other
industries.
The
genes
involved
SM
biosynthesis
are
usually
clustered
adjacent
to
each
into
a
region
known
as
biosynthetic
gene
cluster
(BGC).
recent
advent
diversity
genetic
genomic
technologies
has
facilitated
the
identification
cryptic
or
uncharacterized
BGCs
their
associated
SMs.
However,
there
still
challenges
that
hamper
broader
exploration
industrially
metabolites.
advanced
CRISPR/Cas
system
revolutionized
fungal
engineering
enabled
discovery
novel
compounds.
In
this
review,
we
firstly
introduce
relationships
with
SMs,
followed
by
brief
summary
conventional
strategies
for
engineering.
Next,
range
state-of-the-art
CRISPR/Cas-based
tools
been
developed
review
these
methods
fungi
research
on
Finally,
limitations
systems
discussed
directions
future
proposed
order
expand
improve
efficiency
