SARS-CoV-2 surveillance in US wastewater: Leading indicators and data variability analysis in 2023–2024 DOI Creative Commons
Hannes Schenk, Wolfgang Rauch, Alessandro Zulli

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(11), P. e0313927 - e0313927

Published: Nov. 18, 2024

Wastewater-Based Epidemiology (WBE) has become a powerful tool for assessing disease occurrence in communities. This study investigates the coronavirus 2019 (COVID-19) epidemic United States during 2023–2024 using wastewater data from 189 treatment plants 40 states and District of Columbia. Severe acute respiratory syndrome 2 (SARS-CoV-2) pepper-mild mottle virus normalized SARS-CoV-2 RNA concentration were compared with COVID-19 hospitalization admission at both national state levels. We further investigate temporal features viral abundance, peak timing cross-correlation lag analyses indicating that concentrations precede admissions by to 12 days. Lastly, we demonstrate plant size significant effect on variability measured concentrations. highlights effectiveness WBE as non-invasive, timely resource-efficient monitoring strategy, especially context declining clinical reporting.

Language: Английский

Key considerations for pathogen surveillance in wastewater DOI Creative Commons
Ananda Tiwari, Elena Radu, Norbert Kreuzinger

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 945, P. 173862 - 173862

Published: June 12, 2024

Wastewater surveillance (WWS) has received significant attention as a rapid, sensitive, and cost-effective tool for monitoring various pathogens in community. WWS is employed to assess the spatial temporal trends of diseases identify their early appearances reappearances, well detect novel mutated variants. However, shedding rates vary significantly depending on factors such disease severity, physiology affected individuals, characteristics pathogen. Furthermore, may exhibit differential fate decay kinetics sewerage system. Variable affect detection wastewater. This influence interpretation results conclusions studies. When selecting pathogen WWS, it essential consider it's specific characteristics. If data are not readily available, fate, decay, should be assessed before conducting surveillance. Alternatively, these can compared those similar which available.

Language: Английский

Citations

16
Observations of Respiratory Syncytial Virus (RSV) Nucleic Acids in Wastewater Solids Across the United States in the 2022–2023 Season: Relationships with RSV Infection Positivity and Hospitalization Rates DOI Creative Commons
Alessandro Zulli, Meri R.J. Varkila, Julie Parsonnet

et al.

ACS ES&T Water, Journal Year: 2024, Volume and Issue: 4(4), P. 1657 - 1667

Published: Feb. 29, 2024

Respiratory syncytial virus (RSV) is a leading cause of respiratory illness and hospitalization, but clinical surveillance detects only minority cases. Wastewater could determine the onset extent RSV circulation in absence sensitive case detection, to date, studies wastewater are few. We measured RNA concentrations solids from 176 sites during 2022–2023 season compared those publicly available infection positivity hospitalization rates. Concentrations ranged undetectable 107 copies per gram. concentration aggregated at state national levels correlated with was determined using both rates independent algorithms for 14 states where data were start season. In 4 states, identified same week; 3 preceded onset, 7 occurred after onset. generally peaked week as peaked. Differences peaks versus may reflect inherent differences approaches.

Language: Английский

Citations

13
A retrospective longitudinal study of adenovirus group F, norovirus GI and GII, rotavirus, and enterovirus nucleic acids in wastewater solids at two wastewater treatment plants: solid-liquid partitioning and relation to clinical testing data DOI Creative Commons
Alexandria B. Boehm,

Bridgette Shelden,

Dorothea Duong

et al.

mSphere, Journal Year: 2024, Volume and Issue: 9(3)

Published: Feb. 27, 2024

Enteric infections are important causes of morbidity and mortality, yet clinical surveillance is limited. Wastewater-based epidemiology (WBE) has been used to study community circulation individual enteric viruses panels respiratory diseases, but there limited work studying the concurrent a suite viruses. A retrospective WBE was carried out at two wastewater treatment plants located in California, United States. Using digital droplet polymerase chain reaction (PCR), we measured concentrations human adenovirus group F, enteroviruses, norovirus genogroups I II, rotavirus nucleic acids solids times per week for 26 months (

Language: Английский

Citations

11
Assessment of seasonality and normalization techniques for wastewater-based surveillance in Ontario, Canada DOI Creative Commons
Hadi A. Dhiyebi, Joud Abu Farah, Heather Ikert

et al.

Frontiers in Public Health, Journal Year: 2023, Volume and Issue: 11

Published: Aug. 30, 2023

Introduction Wastewater-based surveillance is at the forefront of monitoring for community prevalence COVID-19, however, continued uncertainty exists regarding use fecal indicators normalization SARS-CoV-2 virus in wastewater. Using three communities Ontario, sampled from 2021–2023, seasonality a viral indicator (pepper mild mottle virus, PMMoV) and utility data to improve correlations with clinical cases was examined. Methods Wastewater samples Warden, Humber Air Management Facility (AMF), Kitchener were analyzed SARS-CoV-2, PMMoV, crAssphage. The PMMoV flow rates examined compared by Season-Trend-Loess decomposition analysis. effects using crAssphage, comparing episode date (CBED) during 2021. Results Seasonal analysis demonstrated that had similar trends AMF peaks January April 2022 low concentrations (troughs) summer months. Warden but more sporadic between troughs concentrations. Flow not correlated very weak ( r = 0.12). Despite differences among sewersheds, unnormalized (raw N1–N2) concentration wastewater n 99–191) strongly CBED 0.620–0.854) Additionally, did significantly reduced Kitchener. crAssphage 29–57) all sites different raw N1–N2 CBED. Discussion Differences seasonal biomarkers caused sewershed characteristics (flow, input, etc.) may play role determining how effective be improving (or not). This study highlights importance assessing influence on normalized or other viruses concern. Fecal used normalize target interest help hinder establishing outcomes wastewater-based needs considered carefully across seasons sites.

Language: Английский

Citations

18
Identification of environmental and methodological factors driving variability of Pepper Mild Mottle Virus (PMMoV) across three wastewater treatment plants in the City of Toronto DOI Creative Commons
Eyerusalem Goitom, Sarah S. Ariano,

Kim Gilbride

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 932, P. 172917 - 172917

Published: May 1, 2024

PMMoV has been widely used to normalize the concentration of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA, influenza, and syncytial virus (RSV) account for variations in fecal content wastewater. is also as an internal RNA recovery control wastewater-based epidemiology (WBE) tests. While potentially useful interpretation WBE data, previous studies have suggested that can be affected by various physico-chemical characteristics There possibility laboratory methods, particularly variability centrifugation steps remove supernatant from pellets cause variability. The goal this study improve our understanding main drivers assessing relationship between concentration, wastewater, methodological approach concentrating wastewater samples. We analyzed 24-hour composite samples collected influent stream three treatment plants (WWTPs) located City Toronto, Ontario, Canada. Samples were 3 5 times per week starting beginning March 2021 mid-July 2023. flow rate was partition data into wet dry weather conditions. Physico-chemical (e.g., total suspended solids (TSS), biological oxygen demand (BOD), alkalinity, electrical conductivity (EC), ammonia (NH

Language: Английский

Citations

6
Metagenomic evaluation of the performance of passive Moore swabs for sewage monitoring relative to composite sampling over time resolved deployments DOI
Gyuhyon Cha, Kevin Zhu,

Jamie Montague Fischer

et al.

Water Research, Journal Year: 2024, Volume and Issue: 253, P. 121269 - 121269

Published: Feb. 9, 2024

Language: Английский

Citations

4
ern: An R package to estimate the effective reproduction number using clinical and wastewater surveillance data DOI Creative Commons
David Champredon, Irena Papst, Warsame Yusuf

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(6), P. e0305550 - e0305550

Published: June 21, 2024

The effective reproduction number, [Formula: see text], is an important epidemiological metric used to assess the state of epidemic, as well effectiveness public health interventions undertaken in response. When text] above one, it indicates that new infections are increasing, and thus epidemic growing, while below one decreasing, so under control. There several established software packages readily available statistically estimate using clinical surveillance data. However, there comparatively few accessible tools for estimating from pathogen wastewater concentration, a data stream cemented its utility during COVID-19 pandemic. We present package ern aims perform estimation number real-world or aggregated user-friendly way.

Language: Английский

Citations

4
Revisiting the Potential Role of Urine in Wastewater Surveillance for COVID-19 and Beyond DOI Creative Commons
Aaron Bivins

Environmental Science & Technology Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Language: Английский

Citations

0
Association between confirmed COVID-19 cases at hospitals and SARS-CoV-2 levels in municipal wastewater during the pandemic and endemic phases DOI Creative Commons
Keisuke Kagami, Masaaki Kitajima, Haruhisa Watanabe

et al.

Environment International, Journal Year: 2025, Volume and Issue: 197, P. 109342 - 109342

Published: Feb. 20, 2025

Language: Английский

Citations

0
Hydrological and physicochemical parameters associated with SARS-CoV-2 and pepper mild mottle virus wastewater concentrations for a large-combined sewer system DOI Creative Commons

Luan Thanh,

Mounia Hachad,

Natasha McQuaid

et al.

Journal of Water and Health, Journal Year: 2025, Volume and Issue: 23(3), P. 413 - 427

Published: Jan. 21, 2025

ABSTRACT During COVID-19, surveillance of SARS-CoV-2 in wastewater has been a promising tool for tracking viral infection at the community level. However, addition to shedding rates within community, concentrations raw are influenced by several environmental factors. This study investigated effects characteristics on quantification and pepper mild mottle virus (PMMoV) large system with combined sewers. Principal component analysis illustrated that water temperature negatively correlates PMMoV wastewater, but flow rate EC highly correlated spring winter. The normalization using enhanced correlation clinical data compared pH, rate, SARS-CoV-2. reduced data. Multiple linear random forest (RF) applied predict given confirmed cases physicochemical parameters. RF regression was best model (R2=0.8), most important variables being followed temperature. is potent predictor presence wastewater. enhances degree reliability between outbreaks monitoring.

Language: Английский

Citations

0