Impact of Sample Storage Time and Temperature on the Stability of Respiratory Viruses and Enteric Viruses in Wastewater DOI Creative Commons
Yuanyuan Qiu,

Richardson Mah,

Logan A. Brand

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2459 - 2459

Published: Nov. 29, 2024

Wastewater-based surveillance (WBS) has been widely used to track SARS-CoV-2 as well many other viruses in communities during the COVID pandemic and post-pandemic. However, it is still not clear how temperature storage time would influence stability of wastewater. In this study, we assessed SARS-CoV-2, pepper mild mottle virus (PMMoV), influenza A (IAV) B (IBV), respiratory syncytial (RSV), enteric raw wastewater stored at room temperature, 4 °C, −20 °C for 3 6 days. PMMoV, IAV, were found be stable up days after storing or °C. RSV more susceptible freeze–thaw cycles compared PMMoV viruses, which relatively Low detection IBV made difficult evaluate impact. Based on our findings, conclude that short-term transportation samples within ambient acceptable majority these viruses. Freezing even short periods recommended WBS The data obtained from study can provide guidance quality assurance purposes operational aspects surveillance.

Language: Английский

Solid–liquid partitioning of dengue, West Nile, Zika, hepatitis A, influenza A, and SARS-CoV-2 viruses in wastewater from across the USA DOI Creative Commons
Laura Roldan-Hernandez, Camila Van Oost, Alexandria B. Boehm

et al.

Environmental Science Water Research & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Limited information is available on the fate of respiratory and arthropod-borne viruses in wastewater.

Language: Английский

Citations

12
Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis DOI Creative Commons
Laura Sancha Domínguez,

Ana Cotos Suárez,

María Sánchez Ledesma

et al.

Diagnostics, Journal Year: 2024, Volume and Issue: 14(9), P. 931 - 931

Published: April 29, 2024

Infectious diseases account for about 3 million deaths per year. The advent of molecular techniques has led to an enormous improvement in their diagnosis, both terms sensitivity and specificity the speed with which a clinically useful result can be obtained. Digital PCR, or 3rd generation is based on series technical modifications that more sensitive techniques, resistant action inhibitors capable direct quantification without need standard curves. This review presents main applications have been developed diagnosis viral, bacterial, parasitic infections potential prospects clinical use this technology.

Language: Английский

Citations

7
Human pathogen nucleic acids in wastewater solids from 191 wastewater treatment plants in the United States DOI Creative Commons
Alexandria B. Boehm, Marlene K. Wolfe, Amanda L. Bidwell

et al.

Scientific Data, Journal Year: 2024, Volume and Issue: 11(1)

Published: Oct. 17, 2024

We measured concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants, influenza A B viruses, syncytial virus, human metapneumovirus, enterovirus D68, parainfluenza types 1, 2, 3, 4a, 4b in aggregate, norovirus genotype II, rotavirus, Candida auris, hepatitis adenovirus, mpox H5 pepper mild mottle virus nucleic acids wastewater solids prospectively at 191 treatment plants 40 states across the United States plus Washington DC. Measurements were made two to seven times per week from 1 January 2022 30 June 2024, depending on plant staff availability. using droplet digital (reverse-transcription-) polymerase chain reaction (ddRT-PCR) following best practices for making environmental molecular biology measurements. These data can be used better understand disease occurrence communities contributing wastewater.

Language: Английский

Citations

6
A Perspective on Wastewater and Environmental Surveillance as a Public Health Tool for Low- and Middle-Income Countries DOI Creative Commons
Mohammad S. Khan, Christian Wurzbacher, Anna Uchaikina

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 238 - 238

Published: Jan. 22, 2025

Geographical variations in infectious diseases create differences public health priorities between high- and low-income countries. Low- middle-income countries (LMICs) face resource constraints that limit adherence to international monitoring standards for wastewater-based epidemiology (WBE). The development of low-cost WBE programs, such as those detect SARS-CoV-2, offers LMICs a promising tool pathogens local concern. In this work, we summarize important wastewater biomarkers their associated challenges, ranging from causing gastroenteritis putative markers plant linked food safety, well antimicrobial resistance. We raise awareness the great potential highlight critical markers, research needs, strategies necessary establish tailored surveillance programs.

Language: Английский

Citations

0
Utilizing Internet Search Trends and Wastewater Surveillance to Identify Infectious Disease Outbreaks in Communities DOI
Alessandro Zulli,

Zoe Zhang,

Madelena Ruedaflores

et al.

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

This study proposes a novel approach for viral infectious disease surveillance using Google Trends data to model wastewater virus concentrations, providing rapid, low-cost method indicating outbreaks. search terms were found correlate strongly with concentrations and clinical cases influenza A respiratory syncytial (R2 = 0.76 0.66). For norovirus mpox, which limited, showed significant correlations concentrations. Three modeling approaches developed: simple linear, stepwise selection, principal component analysis. These models demonstrated strong predictive power both of up 0.66) mpox 0.60) The was validated case documented 2021 outbreak in Hartford, CT, where indicators rose tandem potentially earlier detection than data. offers complementary stream public health decision-making, particularly valuable areas lacking robust testing infrastructure. Limitations include potential confounding factors, such as media coverage the need consider local idioms international applications.

Language: Английский

Citations

0
Evaluation of the Enterovirus serotype monitoring approach for wastewater surveillance of hand foot and mouth disease using secondary epidemiological surveillance data DOI Creative Commons
Sadhana Shrestha, Bikash Malla, Eiji Haramoto

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 969, P. 178896 - 178896

Published: Feb. 26, 2025

Language: Английский

Citations

0
Detection and quantification of human immunodeficiency virus-1 (HIV-1) total nucleic acids in wastewater settled solids from two California communities DOI Open Access
Marlene K. Wolfe,

Bridgette Shelden,

Dorothea Duong

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 13, 2024

Abstract Wastewater surveillance for infectious agents has proved useful in identifying circulation of viruses within populations. We investigated the presence and concentration human immunodeficiency virus (HIV)-1 total nucleic acids (including both viral RNA proviral DNA) wastewater solids. retrospectively measured HIV-1 nucleic-acids two samples per week 26 months at treatment plants serving populations with different prevalences HIV infections San Francisco Santa Clara County, California, USA. detected a majority concentrations ranging from non-detect to 3.9×10 5 cp/g (N=459 total). Concentrations were significantly higher plant population prevalence people living than lower prevalence. The amplified primarily DNA thus represented shedding into wastewater. Additionally, we found that nucleic-acid solids orders magnitude those liquid indicating target preferentially sorbs Whether can be used identify numbers incident cases remains unknown. Additional work on individuals viremia is needed translate measurements quantitative information infections.

Language: Английский

Citations

2
Tracking Norovirus in Tri-County Detroit, MI, Using Wastewater Testing, Syndromic Data, and Online Publicly Available Sources DOI Creative Commons

Heidy Peidro Guzman,

Liang Zhao, Michael J. Swain

et al.

ACS ES&T Water, Journal Year: 2024, Volume and Issue: 4(11), P. 4990 - 5001

Published: Oct. 25, 2024

Human norovirus (HuNoV), the leading cause of acute gastroenteritis in U.S., is voluntarily reported to U.S. Center for Disease Control and Prevention (CDC) when infected individuals do not require hospitalization. Wastewater surveillance can be valuable monitoring HuNoV trends complementing under-reported clinical data. In this study, wastewater samples were collected between January December 2023 from three interceptors Tri-County Detroit, Michigan. genogroup I (GI) II (GII) quantified, highest concentrations observed during winter season. GI GII their sum normalized by water quality parameters fecal indicators. Pearson correlation dynamic time warping (DTW) analysis implemented compare viral concentrations, non-normalized scenarios, with online data sets. Using DTW method, indicators norovirus-positive PCR detection rates Midwest (NPM), followed Google Trends "norovirus", demonstrated most similar patterns. This study highlights importance using multiple sets, including surveillance, identify disease trends, especially diseases.

Language: Английский

Citations

1
Pathogen and indicator trends in southern Nevada wastewater during and after the COVID-19 pandemic DOI Creative Commons
Katherine Crank, Katerina Papp, Casey Barber

et al.

Environmental Science Water Research & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 13, 2024

Wastewater samples spanning three years were analyzed for human enteric pathogens and fecal indicators, highlighting seasonal, geographic, pandemic-related variations of interest water reuse, microbial risk assessment, source tracking.

Language: Английский

Citations

1
Detection and quantification of human immunodeficiency virus-1 (HIV-1) total nucleic acids in wastewater settled solids from two California communities DOI Creative Commons
Marlene K. Wolfe, Meri R.J. Varkila, Alessandro Zulli

et al.

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Wastewater surveillance for infectious agents has proved useful in identifying the circulation of viruses within populations. We investigated presence and concentration human immunodeficiency virus (HIV)-1 total nucleic acids (including both viral RNA proviral DNA) wastewater solids. retrospectively measured HIV-1 two samples per week 26 months at treatment plants serving populations with different prevalences HIV infections San Francisco Santa Clara County, California, USA. detected a majority concentrations ranging from non-detect to 3.9 × 10

Language: Английский

Citations

0