Influenza virus infection and aerosol shedding kinetics in a controlled human infection model DOI Creative Commons
Nishit Shetty, Meredith J. Shephard, Nicole C. Rockey

et al.

Journal of Virology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

ABSTRACT Establishing effective mitigation strategies to reduce the spread of influenza virus requires an improved understanding mechanisms transmission. We evaluated use a controlled human infection model using H3N2 seasonal study critical aspects transmission, including symptom progression and dynamics shedding. Eight volunteers were challenged with A/Perth/16/2009 (H3N2) between July September 2022 at Emory University Hospital. Viral shedding in nasopharynx, saliva, stool, urine, respiratory aerosols was monitored over quarantine period, symptoms tracked until day 15. In addition, environmental swabs collected from participant rooms examine fomite contamination, sera assess seroconversion by hemagglutination inhibition or microneutralization assays. Among eight participants, confirmed six (75%). Infectious viral RNA found multiple physiological compartments, fecal samples, aerosol particles, on surfaces immediate environment. Illness moderate, upper dominating. participants highest loads, antibody titers rose 15 post-inoculation, while low undetectable there little no increase functional titers. These data demonstrate safety utility features transmission manner will inform design future challenge studies focused modeling limiting CLINICAL TRIALS This is registered ClinicalTrials.gov as NCT05332899 . IMPORTANCE kinetics expand our knowledge help aimed human-to-human

Language: Английский

Water Reuse—Retrospective Study on Sustainable Future Prospects DOI Open Access
Morteza Abbaszadegan, Absar Alum, Masaaki Kitajima

et al.

Water, Journal Year: 2025, Volume and Issue: 17(6), P. 789 - 789

Published: March 10, 2025

In recent decades, societies and economies across the globe have started to show signs of stress associated with water shortages. Meeting sustainability benchmarks in arid semi-arid regions has caused reuse be considered a viable alternate source augment existing supply resources. Water reuse, resource recovery, recycling are extensions concept circular economy that been practiced other fields. Globally, U.S. played leadership role development guidance regulations for various applications. Other countries organizations also developed similar programs. This paper aims propose review literature provide broader perspective focusing on most pressing issues such as direct potable backdrop viral pathogens perfluorinated compounds. The global history statutory developments regulate selected contaminants discussed by covering advancement Technological regulatory trends chronicled context emerging linked an imminent social, industrial, agricultural prospectus. proposed high log removal credit is challenging task especially at regular intervals; therefore, treatment requirements must verified ensure public safety. extreme persistence PFAS, their tendency buildup biotic systems, another which requires cost effective efficient technologies. Disparity financial technological capabilities regional or internal stakeholders shared watershed aquifer bottleneck tangible advancements this area. public–private partnerships addressing impending challenges model future direction funding, managing, acceptance.

Language: Английский

Citations

0
Dynamics of SARS-CoV-2 variants in southwest Ohio municipal wastewater DOI
Maitreyi Nagarkar, Scott P. Keely,

Emily Wheaton

et al.

Environmental Science Water Research & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Wastewater surveillance has proven to be a widely useful means for tracking the dynamics of COVID-19, particularly as emphasis on clinical testing and reporting case data continues decline.

Language: Английский

Citations

0
Rapid and extensive SARS-CoV-2 Omicron variant infection wave revealed by wastewater surveillance in Shenzhen following the lifting of a strict COVID-19 strategy DOI
Yinghui Li, Du Chen,

Ziquan Lv

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 949, P. 175235 - 175235

Published: Aug. 3, 2024

Language: Английский

Citations

3
Frequency, Dynamics, and Duration of Fecal Shedding in SARS-CoV-2 Infected Individuals, a Scoping Review DOI Creative Commons

Susan Abunijela,

Timo Greiner, Walter Haas

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

Summary To estimate illness incidence or prevalence from wastewater data, modeling approaches may benefit incorporating fecal shedding parameters. We systematically searched PubMed and a public repository on data included 33 studies meeting at least one of our objectives. Among 32 studies, the proportion SARS-CoV-2-infected individuals with detectable virus in stool ranged 18–100%, pooled 54% (95% CI: 52–56%). Stratification by four clinical severity categories, ranging asymptomatic to critically ill, showed no significant differences among categories (p-value = 0.49). The SARS-CoV-2 RNA was higher children (61%) than adults (53%; p-value 0.02). In half who initially shed stool, it remained for an estimated 22 days post-symptom onset. Three documented viral load kinetics, indicating peak between 3 9. Twenty-five reported maximum durations 2 12 weeks. Our review presents summary frequency, dynamics, duration serve as valuable foundation efforts involving indicators.

Language: Английский

Citations

0
Making waves: Establishing a modeling framework to evaluate novel targets for wastewater-based surveillance DOI
William Chen, Kyle Bibby

Water Research, Journal Year: 2023, Volume and Issue: 245, P. 120573 - 120573

Published: Sept. 2, 2023

Language: Английский

Citations

9
Making waves: Integrating wastewater surveillance with dynamic modeling to track and predict viral outbreaks DOI Creative Commons
Tin Phan, Samantha Brozak, Bruce Pell

et al.

Water Research, Journal Year: 2023, Volume and Issue: 243, P. 120372 - 120372

Published: July 16, 2023

Language: Английский

Citations

8
Wastewater surveillance for bacterial targets: current challenges and future goals DOI Creative Commons
Sarah E. Philo, Kara B. De León, Rachel T. Noble

et al.

Applied and Environmental Microbiology, Journal Year: 2023, Volume and Issue: 90(1)

Published: Dec. 15, 2023

Wastewater-based epidemiology (WBE) expanded rapidly in response to the COVID-19 pandemic. As public health emergency has ended, researchers and practitioners are looking shift focus of existing wastewater surveillance programs other targets, including bacteria. Bacterial targets may pose some unique challenges for WBE applications. To explore current state field, National Science Foundation-funded Research Coordination Network (RCN) on Wastewater Based Epidemiology SARS-CoV-2 Emerging Public Health Threats held a workshop April 2023 discuss needs bacterial surveillance. The methods used were diverse, with twelve different nine listed. Discussions during highlighted adapting identified research gaps four key areas: choosing new relating data human disease incidence prevalence, developing methods, normalizing results. help these gaps, authors steps larger community can take improve bacteria This includes reporting standards method optimization validation programs. Additionally, more work is needed understand shedding patterns potential better relate infections. provide insight into underlying prevalence communities, but much establish methods.IMPORTANCEWastewater was useful tool elucidate burden spread officials interested expanding include many questions remain. NSF-funded Surveillance identify barriers implementing

Language: Английский

Citations

8
Characterizing Spatial Information Loss for Wastewater Surveillance Using crAssphage: Effect of Decay, Temperature, and Population Mobility DOI
Corinne Wiesner-Friedman, Nichole E. Brinkman,

Emily Wheaton

et al.

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(49), P. 20802 - 20812

Published: Nov. 28, 2023

Populations contribute information about their health status to wastewater. Characterizing how that degrades in transit wastewater sampling locations (e.g., treatment plants and pumping stations) is critical interpret responses. In this work, we statistically estimate the loss of fecal contributions from spatially distributed populations at census block group resolution. This was accomplished with a hydrologically hydraulically influenced spatial statistical approach applied crAssphage (

Language: Английский

Citations

7
Predictive power of wastewater for nowcasting infectious disease transmission: A retrospective case study of five sewershed areas in Louisville, Kentucky DOI Creative Commons
Fayette Klaassen, Rochelle H. Holm, Ted Smith

et al.

Environmental Research, Journal Year: 2023, Volume and Issue: 240, P. 117395 - 117395

Published: Oct. 12, 2023

Epidemiological nowcasting traditionally relies on count surveillance data. The availability and quality of such data may vary over time, limiting representation true infections. Wastewater correlates with traditional provide additional value for disease trends.

Language: Английский

Citations

6
A modeling pipeline to relate municipal wastewater surveillance and regional public health data DOI Creative Commons
Katelyn Plaisier Leisman, Christopher Owen, Maria M. Warns

et al.

Water Research, Journal Year: 2024, Volume and Issue: 252, P. 121178 - 121178

Published: Jan. 24, 2024

As COVID-19 becomes endemic, public health departments benefit from improved passive indicators, which are independent of voluntary testing data, to estimate the prevalence in local communities. Quantification SARS-CoV-2 RNA wastewater has potential be a powerful indicator. However, connecting measured community is challenging due high noise typical environmental samples. We have developed generalized pipeline using in- and out-of-sample model selection test ability different correction models reduce variance measurements applied it data collected treatment plants Chicago area. built compared set multi-linear regression models, incorporate pepper mild mottle virus (PMMoV) as population biomarker, Bovine coronavirus (BCoV) recovery control, system flow rate into corrected for concentration. For our with BCoV performed better than those PMMoV, but should used reevaluate any new sources may change across locations, lab methods, disease states. Using best-fit model, we investigated utility leading indicator trends. did this rolling manner other indicators statistically temporal relationship between increases indicators. found that trends often lead predicting surges.

Language: Английский

Citations

1