Probiotics reshape the coral microbiome in situ without detectable off-target effects in the surrounding environment

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: April 9, 2024

Beneficial microorganisms for corals (BMCs), or probiotics, can enhance coral resilience against stressors in laboratory trials. However, the ability of probiotics to restructure microbiome situ is yet be determined. As a first step elucidate this, we inoculated putative probiotic bacteria (pBMCs) on healthy colonies Pocillopora verrucosa Red Sea, three times per week, during 3 months. pBMCs significantly influenced microbiome, while surrounding seawater and sediment remained unchanged. The genera Halomonas, Pseudoalteromonas, Bacillus were enriched probiotic-treated corals. Furthermore, treatment also correlated with an increase other beneficial groups (e.g., Ruegeria Limosilactobacillus), decrease potential pathogens, such as Vibrio. all (treated non-treated) throughout experiment, could not track health improvements protection stress. Our data indicate that healthy, therefore stable, microbiomes restructured situ, although repeated continuous inoculations may required these cases. Further, our study provides supporting evidence that, at studied scale, have no detectable off-target effects near

Language: Английский

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Systematic review of cnidarian microbiomes reveals insights into the structure, specificity, and fidelity of marine associations

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Aug. 14, 2023

Abstract Microorganisms play essential roles in the health and resilience of cnidarians. Understanding factors influencing cnidarian microbiomes requires cross study comparisons, yet plethora protocols used hampers dataset integration. We unify 16S rRNA gene sequences from microbiome studies under a single analysis pipeline. reprocess 12,010 samples 186 studies, alongside 3,388 poriferan, 370 seawater samples, 245 cultured Symbiodiniaceae, unifying ~6.5 billion sequence reads. Samples are partitioned by hypervariable region sequencing platform to reduce variability. This systematic review uncovers an incredible diversity 86 archaeal bacterial phyla associated with Cnidaria, highlights key bacteria hosted across host sub-phylum, depth, microhabitat. Shallow (< 30 m) water Alcyonacea Actinaria characterized highly shared relatively abundant microbial communities, unlike Scleractinia most deeper Utilizing V4 region, we find that composition, richness, diversity, structure primarily influenced phylogeny, sampling ocean body, followed microhabitat date. identify geographical generalist specific Endozoicomonas clades within Cnidaria Porifera. forms framework for understanding governing creates baseline assessing stress dysbiosis.

Language: Английский

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Coral Skeleton Dwelling Endolithic Algae: Ostreobium and Its Biology

Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 41 - 46

Published: Jan. 1, 2025

Language: Английский

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Best practices for generating and analyzing 16S rRNA amplicon data to track coral microbiome dynamics

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: Feb. 20, 2023

Over the past two decades, researchers have searched for methods to better understand relationship between coral hosts and their microbiomes. Data on how coral-associated bacteria are involved in host’s responses stressors that cause bleaching, disease, other deleterious effects can elucidate they may mediate, ameliorate, exacerbate interactions surrounding environment. At same time tracking dynamics reveal previously undiscovered mechanisms of resilience, acclimatization, evolutionary adaptation. Although modern techniques reduced cost conducting high-throughput sequencing microbes, explore composition, function, bacteria, it is necessary entire procedure, from collection sequencing, subsequent analysis be carried out an objective effective way. Corals represent a difficult host with which work, unique steps process microbiome assessment avoid inaccuracies or unusable data libraries, such as off-target amplification sequences. Here, we review, compare contrast, recommend sample collection, preservation, processing (e.g., DNA extraction) pipelines best generate 16S amplicon libraries aim dynamics. We also discuss some basic quality assurance general bioinformatic analyze diversity, taxonomic profiles This review aims generalizable guide interested starting modifying molecular biology aspects research, highlighting practices tricks trade.

Language: Английский

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The Bacterial Microbiome of the Coral Skeleton Algal Symbiont Ostreobium Shows Preferential Associations and Signatures of Phylosymbiosis

Microbial Ecology, Journal Year: 2023, Volume and Issue: 86(3), P. 2032 - 2046

Published: March 31, 2023

Ostreobium, the major algal symbiont of coral skeleton, remains understudied despite extensive research on holobiont. The enclosed nature skeleton might reduce dispersal and exposure residing bacteria to outside environment, allowing stronger associations with algae. Here, we describe bacterial communities associated cultured strains 5 Ostreobium clades using 16S rRNA sequencing. We shed light their likely physical by comparative analysis three datasets generated capture (1) all algae bacteria, (2) enriched tightly attached potential intracellular (3) in spent media. Our data showed that while some may be loosely attached, tend or potentially intracellular. Although colonised diverse preferentially 34 taxa revealing a core microbiome. These include known nitrogen cyclers, polysaccharide degraders, sulphate reducers, antimicrobial compound producers, methylotrophs, vitamin B12 producers. By analysing co-occurrence networks from Porites lutea Paragoniastrea australensis samples, show Ostreobium-bacterial present cultures are also occur natural environment. Finally, our significant congruence between phylogeny community composition its microbiome, largely due phylosymbiotic signal originating taxa. This study offers insight into microbiome reveals preferential warrant further testing functional evolutionary perspectives.

Language: Английский

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Limited influence of seasonality on coral microbiomes and endosymbionts in an equatorial reef

Ecological Indicators, Journal Year: 2023, Volume and Issue: 146, P. 109878 - 109878

Published: Jan. 10, 2023

Coral-associated bacteria and Symbiodiniaceae are critical for maintaining the health of their coral hosts. Factors that known to affect microbiome include biogeography, seasonality, environmental change, but longitudinal studies sorely lacking. Many temporal rarely extend beyond a year, limiting conclusions can be drawn. In this study, we characterised both bacterial communities in three species corals—Merulina ampliata, Pachyseris speciosa, Porites lutea—found an equatorial reef over two-year period. Furthermore, investigated effect monsoon seasons on pattern using metabarcoding methods. We found significant differences through time space, no consistent patterns diversity between conditions (temperature rainfall) were observed. Conversely, did not differ space. Taken together, our results suggest communities, even within stable climate regime, highly dynamic time, affected by monsoonal variations. Finally, network analyses revealed multiple potential biogeochemical processes worked tandem members holobiont support functioning. Longitudinal host-associated microbial drawing relevant about long-term effects change holobiont, directing conservation efforts.

Language: Английский

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A coral-associated actinobacterium mitigates coral bleaching under heat stress

Environmental Microbiome, Journal Year: 2023, Volume and Issue: 18(1)

Published: Nov. 23, 2023

The positive effects of exposing corals to microorganisms have been reported though how the benefits are conferred poorly understood. Here, we isolated an actinobacterial strain (SCSIO 13291) from Pocillopora damicornis with capabilities synthesize antioxidants, vitamins, and antibacterial antiviral compounds supported phenotypic and/or genomic evidence. Strain SCSIO 13291 was labeled 5 (and - 6)-carboxytetramethylrhodamine, succinimidyl ester cell suspension directly inoculated onto coral polyp tissues when nubbins were under thermal stress in a mesocosm experiment. We then visualized labelled bacterial cells analyzed physiological, transcriptome microbiome elucidate effect this on holobiont stress.Subsequent microscopic observations confirmed presence bacterium attached polyps. Addition reduced signs bleaching subjected heat stress. At same time, alterations gene expression, which involved reactive oxygen species light damage mitigation, attenuated apoptosis exocytosis addition metabolite utilization, observed host Symbiodiniaceae populations. In addition, associated community altered more stable ecological network for samples strain.Our results provide insights into putative probiotic that mitigate signs. This study suggests inoculation bacteria can potentially benefit through conferring metabolic activities or indirect mechanisms suppling additional nutrient sources.

Language: Английский

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Variable photosystem II thermal stress responses of reef-building corals Pocillopora indiania and Heliopora coerulea across latitudes from the Mascarene Plateau, Indian Ocean

Deep Sea Research Part II Topical Studies in Oceanography, Journal Year: 2025, Volume and Issue: unknown, P. 105467 - 105467

Published: Feb. 1, 2025

Language: Английский

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Endozoicomonas dominance and Vibrionaceae stability underpin resilience in urban coral Madracis auretenra

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e19226 - e19226

Published: April 15, 2025

Coral resilience varies across species, with some exhibiting remarkable stability and adaptability, often mediated by their associated microbiomes. Given the species-specific nature of coral-microbiome interactions, investigating microbiomes urban-adapted corals provides critical insights into health, dynamics, functioning coral holobionts. In this study, we examined microbiome Madracis auretenra , a Caribbean from Santa Marta, Colombia, contrasting environmental conditions. Over two years, compared healthy stressed colonies distinct reef habitats—urban protected—using 16S rRNA gene sequencing (V4 region) to assess microbial diversity. Our findings revealed richness diversity were primarily influenced seasonal local factors rather than host-specific traits such as interaction algae, health status, or microhabitat. These variations not substantial enough disrupt overall community structure, which remained stable temporal spatial scales. Dominant taxa included Endozoicomonas along Vibrionaceae Rhodobacteraceae, form dense ecological networks. Notably, nutrient oxygen levels emerged key drivers fluctuations, yet populations exhibited exceptional stability. highlight presence well-structured resilient minimal variability, even in urban-influenced environments. We propose that dominance play pivotal role maintaining balance, ultimately contributing M. dynamic habitats.

Language: Английский

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Prokaryotic and eukaryotic microbial communities associated with coral species have high host specificity in the South China Sea

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 867, P. 161185 - 161185

Published: Dec. 27, 2022

Language: Английский

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