Unravelling microalgal-bacterial interactions in aquatic ecosystems through 16S rRNA gene-based co-occurrence networks

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Feb. 16, 2023

Abstract Interactions between microalgae and bacteria can directly influence the global biogeochemical cycles but majority of such interactions remain unknown. 16S rRNA gene-based co-occurrence networks have potential to help identify microalgal-bacterial interactions. Here, we used data from 10 Earth microbiome projects associations in aquatic ecosystems. A high degree clustering was observed modules, indicating densely connected neighbourhoods. Proteobacteria Bacteroidetes predominantly co-occurred with represented hubs most modules. Our results also indicated that species-specificity may be a characteristic microalgal associated microbiomes. Several previously known were recovered our network validating biologically meaningful inferred using this approach. range unknown recognised as co-occurrences Bacillariophyta uncultured Planctomycetes OM190 Deltaproteobacteria order NB1-j . Verrucomicrobia identified key associates due their frequent several taxa. Despite no clear taxonomic pattern, bacterial appeared functionally similar across different environments. To summarise, demonstrated hypothesis-generating framework guide more focused research on associations.

Language: Английский

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Coral Skeleton Dwelling Endolithic Algae: Ostreobium and Its Biology

Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 41 - 46

Published: Jan. 1, 2025

Language: Английский

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Introduction to Coral Reef Microbiome

Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 3 - 6

Published: Jan. 1, 2025

Language: Английский

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Chlamydiae in corals: shared functional potential despite broad taxonomic diversity

ISME Communications, Journal Year: 2024, Volume and Issue: 4(1)

Published: Jan. 1, 2024

Abstract Cnidarians, such as corals and sea anemones, associate with a wide range of bacteria that have essential functions, including nutrient cycling the production antimicrobial compounds. Within cnidarians, can colonize all microhabitats tissues. Among them are obligate intracellular phylum Chlamydiota (chlamydiae) whose impact on cnidarian hosts holobionts, especially corals, remain unknown. Here, we conducted meta-analysis previously published 16S rRNA gene metabarcoding data from cnidarians (e.g. coral, jellyfish, anemones), eight metagenome-assembled genomes (MAGs) coral-associated chlamydiae, one MAG jellyfish-associated chlamydiae to decipher their diversity functional potential. While dataset showed an enormous cnidarian-associated six out nine MAGs were affiliated Simkaniaceae family. The other three assigned Parasimkaniaceae, Rhabdochlamydiaceae, Anoxychlamydiaceae, respectively. All lacked genes necessary for independent existence, lacking any nucleotide or vitamin most amino acid biosynthesis pathways. Hallmark chlamydial genes, type III secretion system, transporters, host interaction, encoded in MAGs. Together these observations suggest lifestyle chlamydiae. No unique found suggesting lack specificity. Additional studies needed understand how interact coral host, microbes holobionts. This first study potential improves our understanding both microbiome range.

Language: Английский

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Activating Biocake Communities Retards Jumps of Transmembrane Pressure in Membrane Bioreactors

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(32), P. 14271 - 14281

Published: July 30, 2024

Sudden jump of transmembrane pressure (TMP) in membrane bioreactors (MBRs), associated with abrupt aggravation fouling, limits practical applications MBRs and calls for effective mitigation strategies. While the TMP is generally related to bacterial activity biocakes, mechanisms underlying remain unclear. Herein, we conducted various backwash protocols different nutrient (e.g., nitrate sodium acetate) loadings on fouled membranes reveal critical role biocakes jump. The filtration tests showed a lower rate backwashed solution (a mixture 180 mg/L NaNO3 200 NaAc, averaged at 1.40 kPa/d) than that tap water (averaged 3.56 kPa/d), implying could be efficiently mitigated by providing sufficient nutrients biocake bacteria. characterization high-nutrient considerably increased viability activity, while reducing biomolecule accumulation membranes. keystone taxa g_Aeromonas o_Chitinophagaceae) network nutrient-enriched communities were involved reduction degradation. Ecological null model analyses revealed deterministic manner mainly shaped availability. Overall, this study highlights significance development provides potential alternatives controlling fouling.

Language: Английский

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Bacterial Microbiota of Ostreobium, the Coral-Isolated Chlorophyte Ectosymbiont, at Contrasted Salinities

Microorganisms, Journal Year: 2023, Volume and Issue: 11(5), P. 1318 - 1318

Published: May 17, 2023

Microscopic filaments of the siphonous green algae Ostreobium (Ulvophyceae, Bryopsidales) colonize and dissolve calcium carbonate skeletons coral colonies in reefs contrasted salinities. Here, we analyzed their bacterial community's composition plasticity response to salinity. Multiple cultures Pocillopora coral-isolated strains from two distinct rbcL lineages representative IndoPacific environmental phylotypes were pre-acclimatized (>9 months) three ecologically relevant reef salinities: 32.9, 35.1, 40.2 psu. Bacterial visualized for first time at filament scale by CARD-FISH algal tissue sections, within siphons, surface or mucilage. Ostreobium-associated microbiota, characterized 16S rDNA metabarcoding cultured thalli corresponding supernatants, structured host genotype (Ostreobium strain lineage), with dominant Kiloniellaceae Rhodospirillaceae (Alphaproteobacteria, Rhodospirillales) depending on lineage, shifted Rhizobiales' abundances salinity increase. A small core microbiota composed seven ASVs (~1.5% ASVs, 19-36% cumulated proportions) was persistent across salinities both genotypes, putative intracellular Amoebophilaceae Rickettsiales_AB1, as well Hyphomonadaceae also detected (Ostreobium-colonized) skeletons. This novel knowledge taxonomic diversity bacteria paves way functional interaction studies holobiont.

Language: Английский

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Microbiota Associated With Ototyphlonemertes Species (Nemertea, Hoplonemertea, Monostilifera, Ototyphlonemertidae) Reveal Evidence of Phylosymbiosis

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(12)

Published: Dec. 1, 2024

ABSTRACT Phylosymbiosis, the association between phylogenetic relatedness of hosts and composition their microbial communities, is a widespread phenomenon in diverse animal taxa. However, generality existence such pattern has been questioned many animals across tree life, including small‐sized aquatic invertebrates. This study aims to investigate communities associated with poorly known marine interstitial nemerteans uncover microbiota diversity assess occurrence phylosymbiosis. Specimens from various Central American sites were analyzed using morphology‐based taxonomy molecular techniques targeting host 18S rRNA gene whereas was by prokaryotic 16S gene. Phylogenetic statistical analyses conducted examine potential effects nemertean taxa sampling locations on host‐associated communities. The results provide compelling evidence phylosymbiosis meiofaunal species, highlighting significant impact genetic microbiome animals. finding supports previous studies that demonstrate how certain species harbor distinct functional ecological implications. Given remarkable animals—spanning numerous phyla varying lifestyles co‐existing same habitat—combined advancements multi‐omics approaches, there promising opportunity deepen our understanding evolutionary interactions throughout life.

Language: Английский

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Chlamydiae as symbionts of photosynthetic dinoflagellates

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Chlamydiae are ubiquitous intracellular bacteria and infect a wide diversity of eukaryotes, including mammals. However, chlamydiae have never been reported to photosynthetic organisms. Here, we describe novel chlamydial genus species, Candidatus Algichlamydia australiensis, capable infecting the dinoflagellate Cladocopium sp. (originally isolated from scleractinian coral). australiensis was confirmed be by fluorescence in situ hybridization confocal laser scanning microscopy temporally stable at population level monitoring its relative abundance across four weeks host growth. Using combination short- long-read sequencing, recovered high-quality (completeness 91.73% contamination 0.27%) metagenome-assembled genome A. australiensis. Phylogenetic analyses show that this taxon represents new species within Simkaniaceae family. possesses all hallmark genes for chlamydiae-host interactions, complete type III secretion system. In addition, IV system is encoded on plasmid has previously observed only three other species. Twenty orthologous groups unique one which structurally similar protein known Cyanobacteria Archaeplastida involved thylakoid biogenesis maintenance, hinting potential interactions with chloroplasts cells. Our study shows symbionts cnidarians, first organism harbor chlamydiae, thereby expanding breadth hosts providing contribution discussion around role establishment primary plastid.

Language: Английский

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Microbiota associated with meiofaunal nemerteans reveals evidence of phylosymbiosis

Authorea (Authorea), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 30, 2023

Phylosymbiosis, the association between phylogenetic relatedness of hosts and composition their microbial communities, is a widespread phenomenon in diverse animal taxa. However, generality existence such pattern has been questioned, there seems evidence against its occurrence small-sized aquatic animals, for which microbiota mostly shaped by local environmental factors. This study aims to investigate communities associated with poorly known marine interstitial nemerteans uncover diversity assess phylosymbiosis. Through comprehensive approach, specimens from various Central American sites were analyzed using morphology-based taxonomy molecular techniques targeting host 18S rRNA gene whereas was prokaryotic 16S gene. Phylogenetic statistical analyses conducted examine potential effects nemertean taxa sampling locations on host-associated communities. The results provide compelling phylosymbiosis meiofaunal nemerteans, emphasizing significant impact genetic microbiome also animals. Additional research needed fully unravel symbiotic relationships as well complex mechanisms that govern across organisms ecological settings. remarkable spanning phyla different lifestyles inhabiting ecosystems, combined advancements multi-omics approaches, offers promising avenue understanding evolutionary interactions throughout tree life.

Language: Английский

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Bacillus Paralicheniformis: A potential candidate for natural antibacterial agents from marine macro-algae associated bacteria.

African Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 6(10), P. 6594 - 6618

Published: July 8, 2024

Antibiotic resistance poses a major global health threat, necessitating new approaches to treat bacterial infections. We isolated bacteria from the brown macroalgae Sargassum swartzii collected along Indian coast. Of 279 isolates, 47.6% showed antibacterial activity against Staphylococcus aureus and Vibrio parahaemolyticus when screened. Ten potent isolates underwent analysis of biosynthetic gene clusters 16S rRNA sequencing. Isolate MP-99 carried polyketide synthase (pks) non-ribosomal peptide synthetase (nrps) genes exhibited effects.

Language: Английский

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