The transcription factor STE12 influences growth on several carbon sources and production of dehydroacetic acid (DHAA) in Trichoderma reesei DOI Creative Commons
Miriam Schalamun, Wolfgang Hinterdobler, Johann Schinnerl

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 11, 2024

Abstract The filamentous ascomycete Trichoderma reesei, known for its prolific cellulolytic enzyme production, recently also gained attention secondary metabolite synthesis. Both processes are intricately influenced by environmental factors like carbon source availability and light exposure. Here, we explore the role of transcription factor STE12 in regulating metabolic pathways T. reesei terms gene regulation, utilization biosynthesis metabolites. We show that is involved cellulase expression growth on sources associated with iron homeostasis. impacts regulation a dependent manner cellulose modulation several CAZyme encoding genes as well metabolism. selectively influences sorbicillinoid trichodimerol, while not affecting bisorbibutenolide, which was shown to be regulated MAPkinase pathway upstream signaling cascade. further report dehydroacetic acid (DHAA) compound antimicrobial properties, subject STE12. conclude, exerts functions beyond development hence contributes balance energy distribution between substrate consumption, reproduction defense.

Language: Английский

Fus3 regulates asexual development and trap morphogenesis in the nematode-trapping fungus Arthrobotrys oligospora DOI Creative Commons

Meihua Xie,

Na Bai, Xuewei Yang

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(8), P. 107404 - 107404

Published: Aug. 1, 2023

Mitogen-activated protein kinase (MAPK) Fus3 is an essential regulator of cell differentiation and virulence in fungal pathogens plants animals. However, the function regulatory mechanism MAPK signaling nematode-trapping (NT) fungi remain largely unknown. NT can specialize formation "traps", important indicator transition from a saprophytic to predatory lifestyle. Here, we characterized orthologous typical fungus Arthrobotrys oligospora using multi-phenotypic analysis multi-omics approaches. Our results showed that plays role asexual growth development, conidiation, stress response, DNA damage, autophagy, secondary metabolism. Importantly, indispensable hyphal fusion, trap morphogenesis, nematode predation. Moreover, constructed networks by means transcriptomic yeast two-hybrid techniques. This study provides insights into development pathogenicity fungi.

Language: Английский

Citations

14
AoMedA has a complex regulatory relationship with AoBrlA, AoAbaA, and AoWetA in conidiation, trap formation, and secondary metabolism in the nematode-trapping fungus Arthrobotrys oligospora DOI
Na Bai,

Meihua Xie,

Qianqian Liu

et al.

Applied and Environmental Microbiology, Journal Year: 2023, Volume and Issue: 89(9)

Published: Sept. 1, 2023

The asexual sporulation of filamentous fungi is an important mechanism for their reproduction, survival, and pathogenicity. In Aspergillus several fungi, BrlA, AbaA, WetA are the key elements a central regulatory pathway controlling conidiation, MedA developmental modifier that regulates temporal expression genes; however, roles largely unknown in nematode-trapping (NT) fungi. Arthrobotrys oligospora representative NT fungus, which can capture nematodes by producing adhesive networks (traps). Here, we characterized function AoMedA three regulators (AoBrlA, AoAbaA, AoWetA) A. gene disruption, phenotypic comparison, multi-omics analyses, as these required conidiation play divergent mycelial development, trap formation, lipid droplet accumulation, vacuole assembly, secondary metabolism. A combined analysis traits transcriptome showed AoWetA involved regulation peroxisome, endocytosis, autophagy. Moreover, yeast one-hybrid AoBrlA regulate AoMedA, AoWetA, whereas AoAbaA AoWetA. Our results highlight AoBrlA, mycelia pathogenicity provide basis elucidating relationship between formation IMPORTANCE Conidiation most common reproductive mode many plays essential role fungal pathogens. Nematode-trapping special group owing to innate abilities digest traps (trapping devices). Sporulation growth reproduction conidia basic components biocontrol reagents diseases caused plant-parasitic nematodes. well-known fungus routinely used model probing interaction this study, functions four (AoMedA, were oligospora. complex was noted; pleiotropic multiple intracellular activities. study first revealed oligospora, contributed helped developing effective

Language: Английский

Citations

13
AoRab7A interacts with AoVps35 and AoVps41 to regulate vacuole assembly, trap formation, conidiation, and functions of proteasomes and ribosomes in Arthrobotrys oligospora DOI

Yingmei Zhu,

Xuewei Yang, Na Bai

et al.

Microbiological Research, Journal Year: 2023, Volume and Issue: 280, P. 127573 - 127573

Published: Dec. 12, 2023

Language: Английский

Citations

9
Multi-omics research in fungus: Current progress and future prospects DOI
Preeti Arya,

Gurprit Sekhon,

Manpreet Kaur

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 161 - 211

Published: Jan. 1, 2025

Language: Английский

Citations

0
The Hog1-Nmd5 signaling pathway regulates asexual development, lipid metabolism, stress response, trap morphogenesis, and secondary metabolism of Arthrobotrys oligospora DOI Creative Commons
Na Zhao,

Qianqian Liu,

Meichen Zhu

et al.

Virulence, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 19, 2025

The high-osmolarity glycerol (HOG) signalling pathway, comprising Ste11/Ssk2/Ssk22 (MAPKKK), Pbs2 (MAPKK), and Hog1 (MAPK), is an important conserved pathway in fungi. However, the functions downstream regulatory factors of nematode-trapping (NT) fungi remain poorly understood. Here, three proteins (AoNmd5, AoPyp1, AoPtp) interacting with were screened a representative NT fungus Arthrobotrys oligospora using yeast screening library verified two-hybrid (Y2H) assay. function AoNmd5 was furtherly characterized by phenotypic comparison, staining technique, multi-omics analyses. essential for vegetative growth, conidial development, trap morphogenesis, nematode predation ability. In addition, played crucial roles endocytosis, lipid metabolism, reactive oxygen species, stress response, autophagy, other metabolic processes. Furthermore, we constructed interaction network based on transcriptomic analysis Y2H, revealing its significant role respiratory chain redox processes as well small GTPase Ran1, which mediates nucleocytoplasmic shuttling. These findings suggest that Hog1-Nmd5 has pleiotropic A. oligospora. This study deepens our understanding HOG importins

Language: Английский

Citations

0
Transcription factor Ste12 regulates the spore maturation and proliferation of silkworm microsporidium Nosema bombycis DOI Open Access

Hongjie Liao,

Bingqian Zhou, Yanchun Zhang

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

As the first identified microsporidium, Nosema bombycis can infect silkworm Bombyx mori to cause pebrine which brings huge economic losses sericulture all over world. The life cycle of is divided into three phases, including infective phase, proliferative phase and sporogonic phase. factor(s) that regulate spore maturation still unknown. Ste12 a fungi-specific transcription factor plays an important regulatory role in fungal development pathogenicity. purpose this study elucidate function homologous (NbSte12) bombycis. Here, we analyzed subcellular localization characteristics NbSte12. It was mainly expressed nucleus immature spores, NbSte12 had transcriptional activation activity, with its C-terminal necessary for ability. DNA affinity purification sequencing (DAP-seq) analysis revealed genes regulated by were involved maturation, cell division, energy metabolism. knockdown gene resulted decrease chitin layer thickness, germination activity proliferation. Then, electrophoretic mobility shift assay (EMSA) quantitative reverse polymerase chain reaction (RT-qPCR) analyses showed could bind promoters CHS7, SWP26, SWP30, PTP1 PTP2 genes, are wall polar tube formation, thereby positively regulating their transcription. indispensable formation layer, proliferation, thus microsporidium © 2025 Society Chemical Industry.

Language: Английский

Citations

0
Transcription Factors in Biocontrol Fungi DOI Creative Commons

Han-Jian Song,

Xiaofeng Li, Xue Pei

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(3), P. 223 - 223

Published: March 15, 2025

Transcription factors are extensively found in fungi and involved the regulation of multiple biological processes, including growth, development, conidiation, morphology, stresses tolerance, virulence, as well production secondary metabolites. Biocontrol is a complex process through which several biocontrol behaviors, such secretion cell wall-degrading enzymes metabolites, regulated by transcription factors. To date, biocontrol-related have been reported fungi, Beauveria bassiana, Clonostachys rosea, Coniothyrium minitans, different species genera Metarhizium, Trichoderma, Arthrobotrys. However, comprehensive reviews summarizing analyzing with potential these scarce. This review begins giving basic overview their functions. Then, role discussed. Lastly, possible approaches for further work on suggested. provides basis elaborating molecular mechanisms context biocontrol.

Language: Английский

Citations

0
Cellular communication and fusion regulate cell fusion, trap morphogenesis, conidiation, and secondary metabolism in Arthrobotrys oligospora DOI Creative Commons
Wenjie Wang, Yankun Liu,

Shipeng Duan

et al.

Microbiological Research, Journal Year: 2023, Volume and Issue: 278, P. 127516 - 127516

Published: Oct. 12, 2023

Language: Английский

Citations

8
AoMae1 Regulates Hyphal Fusion, Lipid Droplet Accumulation, Conidiation, and Trap Formation in Arthrobotrys oligospora DOI Creative Commons
Yankun Liu, Meichen Zhu, Wenjie Wang

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(4), P. 496 - 496

Published: April 21, 2023

Malate dehydrogenase (MDH) is a key enzyme in the tricarboxylic acid (TCA) cycle and essential for energy balance, growth, tolerance to cold salt stresses plants. However, role of MDH filamentous fungi still largely unknown. In this study, we characterized an ortholog (AoMae1) representative nematode-trapping (NT) fungus Arthrobotrys oligospora via gene disruption, phenotypic analysis, nontargeted metabolomics. We found that loss Aomae1 led weakening activity ATP content, remarkable decrease conidia yield, considerable increase number traps mycelial loops. addition, absence also caused obvious reduction septa nuclei. particular, AoMae1 regulates hyphal fusion under low nutrient conditions but not nutrient-rich conditions, volumes sizes lipid droplets dynamically changed during trap formation nematode predation. involved regulation secondary metabolites such as arthrobotrisins. These results suggest has important fusion, sporulation, production, formation, pathogenicity A. oligospora. Our enhance understanding crucial enzymes TCA play development, NT fungi.

Language: Английский

Citations

6
The transcription factor STE12 influences growth on several carbon sources and production of dehydroacetic acid (DHAA) in Trichoderma reesei DOI Creative Commons
Miriam Schalamun, Wolfgang Hinterdobler, Johann Schinnerl

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 26, 2024

The filamentous ascomycete Trichoderma reesei, known for its prolific cellulolytic enzyme production, recently also gained attention secondary metabolite synthesis. Both processes are intricately influenced by environmental factors like carbon source availability and light exposure. Here, we explore the role of transcription factor STE12 in regulating metabolic pathways T. reesei terms gene regulation, utilization biosynthesis metabolites. We show that is involved cellulase expression growth on sources associated with iron homeostasis. impacts regulation a dependent manner cellulose modulation several CAZyme encoding genes as well metabolism. selectively influences sorbicillinoid trichodimerol, while not affecting bisorbibutenolide, which was shown to be regulated MAPkinase pathway upstream signaling cascade. further report dehydroacetic acid (DHAA) compound antimicrobial properties, subject STE12. conclude, exerts functions beyond development hence contributes balance energy distribution between substrate consumption, reproduction defense.

Language: Английский

Citations

1