Microbial Ecology, Journal Year: 2024, Volume and Issue: 87(1)
Published: Dec. 1, 2024
Language: Английский
FEMS Microbiology Reviews, Journal Year: 2023, Volume and Issue: 47(2)
Published: March 1, 2023
Corals live in a complex, multipartite symbiosis with diverse microbes across kingdoms, some of which are implicated vital functions, such as those related to resilience against climate change. However, knowledge gaps and technical challenges limit our understanding the nature functional significance complex symbiotic relationships within corals. Here, we provide an overview complexity coral microbiome focusing on taxonomic diversity functions well-studied cryptic microbes. Mining literature indicate that while corals collectively harbour third all marine bacterial phyla, known symbionts antagonists represent minute fraction this these taxa cluster into select genera, suggesting selective evolutionary mechanisms enabled bacteria gain niche holobiont. Recent advances research aimed at leveraging manipulation increase coral's fitness help mitigate heat stress-related mortality discussed. Then, insights potential through microbiota can communicate modify host responses examined by describing recognition patterns, microbially derived epigenome effector proteins gene regulation. Finally, power omics tools used study highlighted emphasis integrated host-microbiota multiomics framework understand underlying during change-driven dysbiosis.
Language: Английский
Citations
55
Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: 22(8), P. 460 - 475
Published: March 4, 2024
Language: Английский
Citations
47
Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 181 - 195
Published: Jan. 1, 2025
Language: Английский
Citations
2
Trends in Microbiology, Journal Year: 2023, Volume and Issue: 32(3), P. 252 - 269
Published: Sept. 25, 2023
The provision of probiotics benefits the health a wide range organisms, from humans to animals and plants. Probiotics can enhance stress resilience endangered many which are critically threatened by anthropogenic impacts. use so-called 'probiotics for wildlife' is nascent application, field needs reflect on standards its development, testing, validation, risk assessment, deployment. Here, we identify main challenges this emerging intervention provide roadmap validate effectiveness wildlife probiotics. We cover essential inert negative controls in trials investigation probiotic mechanisms action. also suggest alternative microbial therapies that could be tested parallel with application. Our recommendations align approaches used humans, aquaculture, plants concept wildlife.
Language: Английский
Citations
39
Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14
Published: June 2, 2023
More than 20,000 species of prokaryotes (less 1% the estimated number Earth's microbial species) have been described thus far. However, vast majority microbes that inhabit extreme environments remain uncultured and this group is termed "microbial dark matter." Little known regarding ecological functions biotechnological potential these underexplored extremophiles, representing a untapped uncharacterized biological resource. Advances in cultivation approaches are key for detailed comprehensive characterization roles shaping environment and, ultimately, their exploitation, such as extremophile-derived bioproducts (extremozymes, secondary metabolites, CRISPR Cas systems, pigments, among others), astrobiology, space exploration. Additional efforts to enhance culturable diversity required due challenges imposed by culturing plating conditions. In review, we summarize methods technologies used recover environments, while discussing advantages disadvantages associated with each approaches. Additionally, review describes alternative strategies retrieve novel taxa unknown genes, metabolisms, roles, ultimate goal increasing yields more efficient bio-based products. This summarizes unveil hidden microbiome discusses directions future studies matter its applications biotechnology astrobiology.
Language: Английский
Citations
26
mSystems, Journal Year: 2023, Volume and Issue: 8(1)
Published: Jan. 23, 2023
Probiotics are currently the main hope as a potential medicine for corals, organisms that considered marine “canaries of coal mine” and threatened with extinction. Our experiments have proved concept probiotics mitigate coral bleaching can also prevent mortality.
Language: Английский
Citations
19
Microorganisms, Journal Year: 2023, Volume and Issue: 11(4), P. 1080 - 1080
Published: April 20, 2023
Microbial resources from the human gut may find use in various applications, such as empirical research on microbiome, development of probiotic products, and bacteriotherapy. Due to “culturomics”, number pure bacterial cultures obtained has significantly increased since 2012. However, there is still a considerable microbes be isolated cultured. Thus, improve efficiency obtaining microbial gut, some constraints current methods, labor burden, culture condition, targetability, need optimized. Here, we overview general knowledge recent culturomics for microorganisms. Furthermore, discuss optimization several parts including sample collection, processing, isolation, cultivation, which strategies.
Language: Английский
Citations
19
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: June 24, 2024
Abstract The application of beneficial microorganisms for corals (BMC) decreases the bleaching susceptibility and mortality rate corals. BMC selection is typically performed via molecular biochemical assays, followed by genomic screening traits. Herein, we present a comprehensive in silico framework to explore set six putative strains. We extracted high-quality DNA from coral samples collected Red Sea PacBio sequencing. identified traits mechanisms associated with each strain as well proposed new mechanisms, such chemotaxis presence phages bioactive secondary metabolites. prophages two studied strains suggests their possible distribution within bacteria. also detected various metabolites, terpenes, ectoines, lanthipeptides, lasso peptides. These metabolites possess antimicrobial, antifungal, antiviral, anti-inflammatory, antioxidant activities play key roles health reducing effects heat stress, high salinity, reactive oxygen species, radiation. Corals are currently facing unprecedented challenges, our revised can help select more efficient use studies on microbiome rehabilitation, resilience, restoration.
Language: Английский
Citations
6
Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 13
Published: Feb. 20, 2023
Over the past two decades, researchers have searched for methods to better understand relationship between coral hosts and their microbiomes. Data on how coral-associated bacteria are involved in host’s responses stressors that cause bleaching, disease, other deleterious effects can elucidate they may mediate, ameliorate, exacerbate interactions surrounding environment. At same time tracking dynamics reveal previously undiscovered mechanisms of resilience, acclimatization, evolutionary adaptation. Although modern techniques reduced cost conducting high-throughput sequencing microbes, explore composition, function, bacteria, it is necessary entire procedure, from collection sequencing, subsequent analysis be carried out an objective effective way. Corals represent a difficult host with which work, unique steps process microbiome assessment avoid inaccuracies or unusable data libraries, such as off-target amplification sequences. Here, we review, compare contrast, recommend sample collection, preservation, processing (e.g., DNA extraction) pipelines best generate 16S amplicon libraries aim dynamics. We also discuss some basic quality assurance general bioinformatic analyze diversity, taxonomic profiles This review aims generalizable guide interested starting modifying molecular biology aspects research, highlighting practices tricks trade.
Language: Английский
Citations
12
iScience, Journal Year: 2023, Volume and Issue: 26(12), P. 108374 - 108374
Published: Nov. 13, 2023
Most microorganisms found in environmental samples have never been cultured and can often only be explored through molecular or microscopic approaches. Here, we adapt the use of situ diffusion-based devices to culture "yet-to-be-cultured" associated with coral mucus compare this a traditional culturing method. The culturability Pocillopora damicornis increased by 420% 570% diffusion growth chambers microwell chip devices, respectively, compared method tested. obtained cultures represent up 64.4% total diversity amplicon sequence variants (ASVs) P. damicornis. In addition, some previously uncultured microorganisms, such as members family Nitrosopumilaceae halophilic/halotolerant bacteria were cultured. Our results validate alternative microbial strategies coral-associated while significantly increasing previous dark matter.
Language: Английский
Citations
10