Involvement of Transcription Factors and Regulatory Proteins in the Regulation of Carotenoid Accumulation in Plants and Algae

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(48), P. 18660 - 18673

Published: Nov. 21, 2023

Carotenoids are essential for photosynthesis and photoprotection in photosynthetic organisms, which widely used food coloring, feed additives, nutraceuticals, cosmetics, pharmaceuticals. Carotenoid biofortification crop plants or algae has been considered as a sustainable strategy to improve human nutrition health. However, the regulatory mechanisms of carotenoid accumulation still not systematic particularly scarce algae. This article focuses on through factors (transcription proteins), demonstrating complexity homeostasis regulation carotenoids, mainly including transcriptional primary mechanism, subsequent post-translational regulation, cross-linking with other metabolic processes. Different organs different plant/algal species usually have specific biosynthesis, storage, degradation carotenoids response environmental developmental signals. In algae, regulators such MYB, bHLH, MADS, bZIP, AP2/ERF, WRKY, orange proteins can be involved metabolism. And many more regulators, networks, need explored. Our paper will provide basis multitarget multipathway engineering

Language: Английский

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Functional Role and Mutational Analysis of the Phytoene Synthase from the Halophilic Euryarchaeon Haloferax volcanii in Bacterioruberin Biosynthesis

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 18, 2025

Phytoene synthase (PSY) is one of key enzymes in carotenogenesis that catalyze two molecules geranylgeranyl diphosphate to produce phytoene. PSY widespread bacteria, archaea, and eukaryotes. Currently, functional role catalytic mechanism archaeal homologues have not been fully clarified due the limited reports. Herein, we identified a rate-limiting from halophilic euryarchaeon Haloferax volcanii (HVO-PSY) bacterioruberin biosynthesis dissected roles its seven conserved residues. Compared with parental H. strain, recombinant HVO-PSY strain had increased yields, confirming enzyme volcanii. Additionally, optimized culture condition for strain: 150 g/L NaCl, 45 °C, pH 6.5-7.5. Mutational data demonstrate residues D47, D51, D110, D168, D172, R178 are essential catalysis since these mutant strains harboring mutations reduced yields relative wild-type strain. Overall, our findings verified catalysis.

Language: Английский

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Comparative genomics and evolutionary insights into zeaxanthin biosynthesis in two novel Flavobacterium species

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 23, 2025

During the screening of pigment-producing microbes from domestic sources, 102 yellow- or orange-pigmented bacteria were isolated. Among these, two novel Flavobacterium strains, F. sedimentum SUN046T and fluvius SUN052T, identified as zeaxanthin producers. A polyphasic taxonomic characterization, combined with comparative genomic analysis 45 species, was conducted to determine their positions explore potential evolutionary relationships in biosynthesis. Both strains utilized mevalonic acid (MVA) pathway possessed crt gene cluster (crtB, crtI, crtY/crtYcd, crtZ). Strain exhibited unique features carotenoid biosynthesis pathway, notably absence HMG-CoA synthase (HMGCS) upper MVA presence rare lycopene β-cyclase crtYcd, which is uncommon among bacteria. The CrtYcd a single active site direct lycopene-binding modes. Conversely, CrtY SUN052T multiple sites, flavin adenine dinucleotide (FAD) dependent. These structural differences has impacted catalytic efficiencies, evidenced by yields 6.49 µg/mL 13.23 SUN052T. Variations biosynthetic other species also observed. findings suggest that both represent valuable new resources for production provide foundational insights biotechnological applications involving genus Flavobacterium, highlighting genetic complexity microbial

Language: Английский

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Recent Advances on Key Enzymes of Microbial Origin in the Lycopene Biosynthesis Pathway

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(35), P. 12927 - 12942

Published: Aug. 23, 2023

Lycopene is a common carotenoid found mainly in ripe red fruits and vegetables that widely used the food industry due to its characteristic color health benefits. Microbial synthesis of lycopene gradually replacing traditional methods plant extraction chemical as more economical productive manufacturing strategy. The biosynthesis typical multienzyme cascade reaction, it important understand characteristics each key enzyme involved how they are regulated. In this paper, catalytic enzymes pathway related studies first discussed detail. Then, strategies applied synthesis, including fusion proteins, screening, combinatorial engineering, CRISPR/Cas9-based gene editing, DNA assembly, scaffolding technologies purposefully illustrated compared terms both emerging regulatory strategies. Finally, future developments options for similar secondary metabolites also discussed.

Language: Английский

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Optimization of Culture Conditions to Alleviate Growth Inhibition by Fulvic Acid in Microalgae Dunaliella Bardawil to Further Promote Carotenoid Accumulation

Published: Jan. 1, 2024

Natural carotenoids from microalgae have received more attention as an alternative source. In this study, fulvic acid (FA), a plant growth regulator, were used to promote carotenoid accumulation in Dunaliella bardawil rich lutein. However, the addition of FA promoted pigment but also has inhibitory effect on biomass, therefore optimization culture conditions was performed further improve production. At high light intensity (10,000 lx), inhibition by alleviated. The contents lutein, α-carotene and β-carotene under treatment 400 mg/L with much higher than those control same intensity. addition, due promoting 1-aminocyclopropane-1-carboxylic (ACC) D. bardawil, two-stage cultivation comprising ACC simultaneously increase biomass accumulation. increased remained that even presence FA, treatments. may be enhanced storage capacity associated lipids up-regulated transcription levels carotenogenic genes (DbLCYB, DbLCYE, DbBCH, DbCYP97A DbCYP97C).

Language: Английский

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Effects of aromatic amino acids on callus growth and accumulation of secondary metabolites in amaranth

Tropical Plants, Journal Year: 2024, Volume and Issue: 3(1), P. 0 - 0

Published: Jan. 1, 2024

Language: Английский

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Dual Roles of Two Malic Enzymes in Lipid Biosynthesis and Salt Stress Response in Dunaliella salina

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 31, 2023

Triacylglycerols (TAG) from microalgae can be used as feedstocks for biofuel production to address fuel shortages. Most of the current research has focused on enzymes involved in TAG biosynthesis. In this study, effects malic enzyme (ME), which provides precursor and reducing power biosynthesis, biomass lipid accumulation its response salt stress Dunaliella salina were investigated. The overexpression DsME1 DsME2 improved production, reached 0.243 0.253 g/L 30.5 36.3% higher than wild type, respectively. transcript levels increased with increasing concentration (0, 1, 2, 3, 4.5 mol/L NaCl), indicating that DsMEs participated D. salina. It was found cis-acting elements associated present promoters two DsMEs. deletion MYB binding site (MBS) promoter confirmed MBS drives expression participate osmotic regulation conclusion, MEs are critical play pivotal roles regulation.

Language: Английский

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Functional Characterization of a CruP-Like Isomerase in Dunaliella

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(17), P. 10005 - 10013

Published: April 16, 2024

is a marine unicellular green algal that produces large amounts of β-carotene and model organism for studying the carotenoid synthesis pathway. However, there are still many mysteries about enzymes

Language: Английский

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Modelamientos in silico un mecanismo de enseñanza – aprendizaje en contexto virtual a estudiantes de biotecnología en pandemia COVID-19

LATAM Revista Latinoamericana de Ciencias Sociales y Humanidades, Journal Year: 2024, Volume and Issue: 5(4)

Published: June 17, 2024

La crisis provocada por la pandemia COVID-19 condujo a las universidades reestructurar rápidamente sus programas de estudios. El área experimental ciencias biológicas se vio afectada ya que los laboratorios presenciales son parte integral educación superior. Este hecho, llevó materia biotecnología adopte su programa estudios virtuales y modelamientos in silico. De esta manera el objetivo este trabajo es mejorar formación académica en tiempos críticos, utilizando silico como un mecanismo enseñanza-aprendizaje contexto con estudiantes biotecnología. Para simular procesos biotecnológicos Dunaliella salina tomó modelo gen psy, amplificación del PCR, resguardo gen, caracterización enzima biosíntesis carotenoides. Las aptitudes conocimientos adquiridos fueron evaluados través una encuesta 156 cursaron biotecnología-UPSE. Los demostraron psy comparte alta similitud cobertura superior 81% otras especies vegetales, resultó producto 1218 pb. Mientras estructura proteica alcanzó 0,69 QMING activación cascada señalización celular demostró 5 genes involucrados Finalmente, percepción positiva favor 70,83%. aplicación permitieron biológicos experimentales comprender efectiva no presencialidad.

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Enhancing carotenoid accumulation in Dunaliella bardawil by combined treatments with fulvic acid and optimized culture conditions

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 217, P. 109206 - 109206

Published: Oct. 16, 2024

Language: Английский

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