Alterations of the intestinal microbiome and metabolome in women with rheumatoid arthritis DOI Creative Commons
Haifeng Yun, Xinxin Wang,

Changjiang Wei

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: June 29, 2023

Abstract Objectives: Rheumatoid arthritis (RA) is more common in women, and many reports of sex differences have been reported various aspects RA. However, there has a lack specific research on women's gut flora. To assess the association between flora RA patients, this study combined microbiome with metabolomics. Methods: Fecal samples from patients healthy controls were collected for 16S rRNA sequencing. Nontargeted liquid chromatography‒mass spectrometry was used to detect metabolites fecal samples. We comprehensively analytical methods reveal changes intestinal female patients. Results: The significantly different that women. abundance Bacteroides, Megamonas Oscillospira higher while Prevotella, Gemmiger Roseburia lower than Gemmiger, Bilophila Odoribacter represented large microflora women could be as potential microorganisms diagnosis. Fatty acid biosynthesis terms metabolic pathways. There degrees correlation metabolites. Lys-Phe-Lys heptadecasphin-4-enine can markers an extremely significant positive Megamonas, Dialister rheumatoid factors (RF), which found first time. Conclusions: These findings indicated alterations these metabolome may contribute diagnosis treatment

Language: Английский

Probiotics and Amelioration of Rheumatoid Arthritis: Significant Roles of Lactobacillus casei and Lactobacillus acidophilus DOI Creative Commons
Alok Kumar Paul, Anita Paul, Rownak Jahan

et al.

Microorganisms, Journal Year: 2021, Volume and Issue: 9(5), P. 1070 - 1070

Published: May 16, 2021

Rheumatoid arthritis is a chronic autoimmune disorder that can lead to disability conditions with swollen joints, pain, stiffness, cartilage degradation, and osteoporosis. Genetic, epigenetic, sex-specific factors, smoking, air pollution, food, oral hygiene, periodontitis,

Language: Английский

Citations

47
A catalog of 48,425 nonredundant viruses from oral metagenomes expands the horizon of the human oral virome DOI
Shenghui Li, Ruochun Guo, Yue Zhang

et al.

iScience, Journal Year: 2022, Volume and Issue: 25(6), P. 104418 - 104418

Published: May 18, 2022

Language: Английский

Citations

35
Meta-analysis of fecal viromes demonstrates high diagnostic potential of the gut viral signatures for colorectal cancer and adenoma risk assessment DOI Creative Commons
Fang Chen, Shenghui Li, Ruochun Guo

et al.

Journal of Advanced Research, Journal Year: 2022, Volume and Issue: 49, P. 103 - 114

Published: Oct. 2, 2022

Viruses have been reported as inducers of tumorigenesis. Little studies explored the impact gut virome on progression colorectal cancer. However, there is still a problem with repeatability viral signatures across multiple cohorts.The present study aimed to reveal repeatable vial cancer and adenoma patients decipher potential markers in disease risk assessment for diagnosis.1,282 available fecal metagenomes from 9 published were collected. A catalog was constructed via reference-independent approach. Viral identified by cross-cohort meta-analysis used build predictive models based machine learning algorithms. New samples collected validate generalization models.The composition drastically altered compared healthy, evidenced changes some Siphoviridae Myoviridae viruses enrichment Microviridae, whereas variation relatively low. Cross-cohort 405 differential cancer, including several phages Porphyromonas, Fusobacterium, Hungatella that enriched control-enriched Ruminococcaceae phages. In discovery cohorts, optimal model obtained an average area under curve 0.830 discriminating controls. This also showed consistently high accuracy 2 independent validation cohorts (optimal curve, 0.906). Gut analysis 88 achieved 0.772 controls.Our findings demonstrate characteristics highlight virus-bacterial synergy The may be new targets treatment. addition, power prediction suggest biomarkers non-invasive diagnostic tests adenoma.

Language: Английский

Citations

30
Gut bacteriome, mycobiome and virome alterations in rheumatoid arthritis DOI Creative Commons

Sonali Dagar,

Jagdeep Singh,

Aastha Saini

et al.

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 13

Published: Jan. 9, 2023

Rheumatoid arthritis (RA) is a chronic destructive autoimmune disease of the joints which causes significant pain, functional disability, and mortality. Although aberrant immune cell activation induced by imbalance between T helper Th1/Th17 Treg cells implicated in RA development, its etiopathogenesis remains unclear. The presence mucosal inflammation systemic IgA-isotype-autoantibodies (anti-citrullinated peptide antibodies rheumatoid factor) pre-clinical supports origin hypothesis involving altered microbiota development. gut comprises diverse bacteria, fungal viral components, are critical developing host immunity. Alterations microbial abundance known to exacerbate or attenuate responses microenvironment subsequently affecting joints. Further, these changes can provide biomarkers for activity outcome RA. Most research till date has been focused on describing bacterial components Studies mycobiome virome relatively new burgeoning field. Given paucity specific studies RA, this review, discusses recent findings alterations bacterial, fungal, as well their role regulating spectrum immune-pathogenic events occurring might be explored future potential therapeutic target. we an overview inter-kingdom interactions fungi, viruses current understanding modulation managing also summarised.

Language: Английский

Citations

20
Dysbiotic Oral and Gut Viromes in Untreated and Treated Rheumatoid Arthritis Patients DOI Creative Commons
Ruochun Guo, Shenghui Li, Yu Zhang

et al.

Microbiology Spectrum, Journal Year: 2022, Volume and Issue: 10(5)

Published: Aug. 30, 2022

Rheumatoid arthritis (RA) is influenced by oral and gut bacteria; however, much less known about the relationship between or viromes RA. Here, we performed whole-oral- whole-gut-virome analyses based on shotgun sequencing of 497 samples. A comparative analysis in healthy controls untreated treated RA patients was performed, system interaction networks among viruses, bacteria, RA-associated clinical indices were constructed to address potential virome principal-coordinate analysis, distance-based redundancy permutational multivariate Spearman correlation coefficient random-forest model analysis. The results showed that could be profiled dental plaque, saliva, fecal samples, which saliva had highest within-sample diversity. Importantly, significantly different diversities compositions (i.e., plaque saliva) observed not only but also patients, yet there relatively minor differences viromes. Furthermore, understand how these viruses affected bacteriome, a virus-bacterium network from samples patients. Additionally, some taxa, including Lactococcus phage (vOTU70), Bacteroides vulgatus, lactis, Escherichia coli, Neisseria elongata, correlated with RA-related indices. Whole-virome illustrated role affecting our body either directly via characterized neglected new candidates contributing development IMPORTANCE Our demonstrated community variation In perturbance viral composition microbes might involved pathogenicity findings this study expand knowledge communities may contribute research correlations other diseases.

Language: Английский

Citations

26
Gut virome-wide association analysis identifies cross-population viral signatures for inflammatory bowel disease DOI Creative Commons
Xiangge Tian, Shenghui Li, Chao Wang

et al.

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: July 18, 2024

Abstract Background The gut virome has been implicated in inflammatory bowel disease (IBD), yet a full understanding of the IBD patients, especially across diverse geographic populations, is lacking. Results In this study, we conducted comprehensive virome-wide association study Chinese cohort 71 patients (15 with Crohn’s and 56 ulcerative colitis) 77 healthy controls via viral-like particle (VLP) bulk sequencing their feces. By utilizing an integrated virus catalog tailored to virome, revealed fundamental alterations patients. These characterized 139 differentially abundant viral signatures, including elevated phages predicted infect Escherichia , Klebsiella Enterococcus_B Streptococcus Veillonella species as well IBD-depleted targeting Prevotella Ruminococcus_E Bifidobacterium Blautia . Remarkably, these signatures demonstrated high consistency populations such those Europe USA, emphasizing significance broad relevance context. Furthermore, fecal transplantation experiments verified that colonization IBD-characterized viruses can modulate experimental colitis mouse models. Conclusions Building upon insights into identified potential biomarkers for prognosis therapy laying foundation further exploration viromes related conditions.

Language: Английский

Citations

5
Alterations in the gut virome in patients with ankylosing spondylitis DOI Creative Commons
Chen Li, Yan Zhang,

Qiulong Yan

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: March 30, 2023

Introduction Ankylosing spondylitis (AS), a chronic autoimmune disease, has been linked to the gut bacteriome. Methods To investigate characteristics of virome in AS, we profiled viral community 193 AS patients and 59 healthy subjects based on metagenome-wide analysis fecal metagenomes from two publicly available datasets. Results revealed significant decrease richness considerable alteration overall structure. At family level, had an increased abundance Gratiaviridae Quimbyviridae decreased Drexlerviridae Schitoviridae . We identified 1,004 differentially abundant operational taxonomic units (vOTUs) between controls, including higher proportion AS-enriched Myoviridae viruses control-enriched Siphoviridae viruses. Moreover, vOTUs were more likely infect bacteria such as Flavonifractor , Achromobacter Eggerthellaceae whereas be Blautia Ruminococcus Collinsella Prevotella Faecalibacterium bacteriophages. Additionally, some functional orthologs differed significantly frequency vOTUs, suggesting role these AS-associated trained classification models signatures discriminate with optimal area under receiver operator characteristic curve (AUC) up 0.936, clinical potential for diagnosing AS. Discussion This work provides novel insight into virome, findings may guide future mechanistic therapeutic studies other diseases.

Language: Английский

Citations

11
Alterations of the intestinal microbiome and metabolome in women with rheumatoid arthritis DOI
Haifeng Yun, Xinxin Wang,

Changjiang Wei

et al.

Clinical and Experimental Medicine, Journal Year: 2023, Volume and Issue: 23(8), P. 4695 - 4706

Published: Aug. 12, 2023

Language: Английский

Citations

11
Metagenome‐Based Characterization of the Gut Virome Signatures in Patients With Gout DOI

Liansha Huang,

Changming Chen, Jinxin Meng

et al.

Journal of Medical Virology, Journal Year: 2025, Volume and Issue: 97(4)

Published: April 1, 2025

ABSTRACT The gut microbiome has been implicated in the development of autoimmune diseases, including gout. However, role virome gout pathogenesis remains underexplored. We employed a reference‐dependent approach to analyze fecal metagenomic data from 102 patients (77 discovery cohort and 25 validation cohort) 86 healthy controls (HCs) (63 23 each cohort). A subset provided longitudinal samples at Weeks 2, 4, 24. Our analysis revealed significant alterations patients, reduced viral richness shifts family composition. Notably, Siphoviridae , Myoviridae Podoviridae were depleted, while Quimbyviridae Retroviridae Schitoviridae enriched patients. identified 359 operational taxonomic units (vOTUs) associated with Enriched vOTUs predominantly consisted Fusobacteriaceae, Bacteroidaceae, Selenomonadaceae phages, control‐enriched included Ruminococcaceae, Oscillospiraceae, Enterobacteriaceae phages. Longitudinal that substantial proportion these signatures remained stable over 6 months. Functional profiling highlighted enrichment auxiliary metabolic genes, suggesting potential interactions between viruses host bacteria. effectively discriminated HCs, high classification performance cohort. This study provides first comprehensive characterization gout, revealing its disease highlighting virome‐based as promising biomarkers for diagnosis future therapeutic strategies.

Language: Английский

Citations

0
Dual regulatory effects of gut microbiota and their metabolites in rheumatoid arthritis: balancing pathogenic and protective mechanisms DOI Creative Commons
Xingwen Xie, Xin Chen, Xuetao Wang

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 30, 2025

Rheumatoid arthritis is a chronic autoimmune disorder characterized by destructive, symmetric joint inflammation and synovitis, resulting in substantial disability that profoundly compromises patients’ quality of life. Its pathogenesis encompasses complex interactions between genetic environmental factors. Recent advances bacterial DNA sequencing technologies have uncovered significant correlation the human gut microbiota composition rheumatoid progression. Growing clinical experimental evidence establishes gut-joint axis as crucial mediator pathogenesis. Comprehensive investigation microbial communities their metabolites’ influence on mechanisms, coupled with elucidation microbiome’s bidirectional regulatory effects disease development, not only deepens our understanding pathological processes but also theoretical framework for developing novel diagnostic biomarkers personalized therapeutic interventions to enhance patient outcomes.

Language: Английский

Citations

0