Whole-Genome Sequencing and Metagenomics Reveals Diversity and Prevalence of Soil Listeria spp. in the Nantahala National Forest DOI Creative Commons
Jia Wang,

Claire N. Schamp,

Lauren K. Hudson

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 31, 2023

Abstract Background. Listeria spp. are widely distributed environmental bacteria associated with human foodborne illness. Whole-genome sequencing (WGS) of Listeria monocytogenes clinical isolates is the current standard for surveillance and identifying outbreaks. WGS Listeria can assist in understanding diversity species. The capability precise detection characterization strains natural environment important transmission routes that lead to food contamination Recently, metagenomic has emerged as a promising tool direct samples. objective this study was use practical hybrid approach provide valuable information on occurrence soil environments southern Appalachian region United States. Results. This involved sampling from four locations across varying altitudes Nantahala National Forest. Forty-two 12 metagenomes bacterial communities were obtained. Analysis genomes revealed 14 distinct strains, representing predominant species isolated L. cossartiae subsp. cayugensis (n = 8), other including 3), ‘L. swaminathanii’ 1), marthii booriae 1). Most 13) lower (1,500 or 2,500 ft), while strain two different altitudes. Metagenomic analysis demonstrated decline both community relative abundance increasing altitude. Of factors assessed, pH cation exchange capacity significantly positively correlated detected by metagenomics. Conclusions. By integrating culture-independent metagenomics culture-based WGS, provides insights into prevalence These findings contribute knowledge distribution offer potential method tracking pathogens.

Language: Английский

Human Listeriosis DOI
Marion Koopmans, Matthijs C. Brouwer, José A. Vázquez‐Boland

et al.

Clinical Microbiology Reviews, Journal Year: 2022, Volume and Issue: 36(1)

Published: Dec. 8, 2022

Listeria monocytogenes is a Gram-positive facultative intracellular pathogen that can cause severe invasive infections upon ingestion with contaminated food. Clinically, listerial disease, or listeriosis, most often presents as bacteremia, meningitis meningoencephalitis, and pregnancy-associated manifesting miscarriage neonatal sepsis. Invasive listeriosis life-threatening main of foodborne illness leading to hospital admissions in Western countries. Sources contamination be identified through international surveillance systems for bacteria strains' genetic data sharing. Large-scale whole genome studies have increased our knowledge on the diversity evolution L. monocytogenes, while recent pathophysiological investigations improved mechanistic understanding listeriosis. In this article, we present an overview human particular focus relevant features causative bacterium, epidemiology, risk groups, pathogenesis, clinical manifestations, treatment prevention.

Language: Английский

Citations

125
Taxonomy, ecology, and relevance to food safety of the genus Listeria with a particular consideration of new Listeria species described between 2010 and 2022 DOI Creative Commons
Renato H. Orsi, Jingqiu Liao, Catharine R. Carlin

et al.

mBio, Journal Year: 2023, Volume and Issue: 15(2)

Published: Dec. 21, 2023

Since 2010, the genus Listeria has had addition of 22 new species that more than tripled number identified until 2010. Sixteen these are distantly related to type species, monocytogenes, and several present phenotypes distinguish them from classical (L. innocua, ivanovii, seeligeri, welshimeri, grayi). These newly described also show is genetically diverse previously estimated. While future studies surveys needed clarify distribution at least some may not be widely spread, while other frequently found spread human-related settings (e.g., farms processing facilities), others adapted specific environmental habitats. Here, we review taxonomic, phylogenetic, ecological characteristics since 2010 re-iterate suggestion re-classification into three genera: Murraya, Mesolisteria, Paenilisteria. We provide a current detection issues relevance food safety identification species. For example, non-pathogenic could misidentified as pathogen L. based on methods do target monocytogenes-specific virulence genes/factors, leading unnecessary product recalls. Moreover, eight in proposed Mesolisteria good indicators conditions allow monocytogenes grow unable low temperatures.

Language: Английский

Citations

14
Phylogenomic analyses of the Listeriaceae family support species reclassification and proposal of a new family and new genera DOI
Khaoula Bouznada, Hadj Ahmed Belaouni, Rafika Saker

et al.

Antonie van Leeuwenhoek, Journal Year: 2024, Volume and Issue: 118(1)

Published: Oct. 10, 2024

Language: Английский

Citations

3
Prevalence, Serotypes, Antimicrobial Resistance and Biofilm-Forming Ability of Listeria monocytogenes Isolated from Bulk-Tank Bovine Milk in Northern Greece DOI Creative Commons
Apostolos S. Angelidis,

Afroditi S. Grammenou,

Charalampos Kotzamanidis

et al.

Pathogens, Journal Year: 2023, Volume and Issue: 12(6), P. 837 - 837

Published: June 17, 2023

The prevalence of Listeria monocytogenes in bovine bulk-tank milk (BTM) Greece has not been previously investigated. aim the study was to estimate L. BTM and characterize isolates terms carriage genes encoding for pathogenic determinants, assess isolates' biofilm-forming ability determine their susceptibility against 12 antimicrobials. Samples (n = 138) were obtained from farms located throughout Northern analyzed qualitatively quantitatively monocytogenes. Five samples (3.6%) tested positive pathogen's populations these below 5 CFU/mL. Most belonged molecular serogroup "1/2a, 3a". All carried virulence inlA, inlC, inlJ, iap, plcA hlyA, but actA detected only three isolates. displayed weak moderate distinct antimicrobial resistance profiles. characterized as multidrug resistant, with penicillin clindamycin being a common feature. Considering that constitutes serious public health threat, key findings study, related resistance, highlight importance continued monitoring pathogen farm animals.

Language: Английский

Citations

7
Listeriosis: Characteristics, Occurrence in Domestic Animals, Public Health Significance, Surveillance and Control DOI Creative Commons
Ana Končurat, Tomislav Sukalić

Microorganisms, Journal Year: 2024, Volume and Issue: 12(10), P. 2055 - 2055

Published: Oct. 12, 2024

Listeriosis is a dangerous zoonosis caused by bacteria of the genus

Language: Английский

Citations

2
Phenotypic characterization and analysis of complete genomes of two distinct strains of the proposed species “L. swaminathanii” DOI Creative Commons
Lauren K. Hudson,

Harleen K. Chaggar,

Claire N. Schamp

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: June 1, 2022

Abstract Recently, a new Listeria species, “ swaminathanii ”, was proposed. Here, we phenotypically and genotypically characterize two additional strains that were previously obtained from soil samples compare the results to type strain. Complete genomes for both assembled hybrid Illumina Nanopore sequencing reads annotated. Further genomic analysis including average nucleotide identity (ANI) detection of mobile genetic elements genes interest (e.g., virulence-associated) conducted. The showed 98.7–98.8% ANI with UTK C1-0015 genome contained partial monocin locus plasmid, while C1-0024 full prophage. Phenotypic characterization consistent those performed on proposed strain conducted assess consistency phenotypes across greater diversity species (n = 3 instead n 1). Only few findings notably different strain, such as catalase activity, glycerol metabolism, starch growth at 41 °C. This study further expands our understanding this newly sensu stricto species.

Language: Английский

Citations

10
A novel RofA-family transcriptional regulator, GadR, controls the development of acid resistance in Listeria monocytogenes DOI Creative Commons
Jialun Wu, Olivia McAuliffe, Conor P. O’Byrne

et al.

mBio, Journal Year: 2023, Volume and Issue: 14(6)

Published: Oct. 26, 2023

The ability to survive the acidic conditions found in stomach is crucial for food-borne pathogen

Language: Английский

Citations

3
Detection of Listeria Species by Conventional Culture-Dependent and Alternative Rapid Detection Methods in Retail Ready-to-Eat Foods in Turkey DOI
Emine Dinçer

Journal of Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 34(2), P. 349 - 357

Published: Nov. 16, 2023

Citations

3
Selection of mutant Listeria phages under food-relevant conditions can enhance application potential DOI

Claire N. Schamp,

Nitin Dhowlaghar, Lauren K. Hudson

et al.

Applied and Environmental Microbiology, Journal Year: 2023, Volume and Issue: 89(10)

Published: Oct. 6, 2023

ABSTRACT Bacteriophages are viruses that infect and kill bacteria. Currently, phage products available for the control of pathogen Listeria monocytogenes in food United States. In this study, we explore whether experimental evolution can be used to generate phages with improved abilities function under specific food-relevant conditions. Ultra-pasteurized oat whole milk were chosen as test matrices they represent different groups, yet have similar physical traits macronutrient composition. We showed (i) wild-type LP-125 infection kinetics two (ii) has a significantly higher burst size milk. From this, attempted evolve Ancestral was passaged through 10 rounds amplification Plaque-purified DNA samples from milk-selected isolated sequenced, mutations present identified. found nonsynonymous substitutions LP125_108 LP125_112 genes, which encode putative baseplate-associated glycerophosphoryl diester phosphodiesterase baseplate protein, respectively. Protein structural modeling substituted amino acids mutant predicted localize surface-exposed helices on corresponding structures, might affect surface charge proteins their interaction bacterial cell. The containing mutation adsorbed faster than ancestral both Follow-up experiments suggest fat content may key factor expression phenotype mutation. IMPORTANCE one tools foodborne pathogen, . Phage must work broad range conditions an effective L. Here, show new phenotypes useful approach select more efficiently binds is grown these milks necessary phenotype. Our findings better performance This potential support development condition-specific phage-based biocontrols industry.

Language: Английский

Citations

2
Soil Collected from a Single Great Smoky Mountains Trail Contains a Diversity of Listeria monocytogenes andListeriaspp. DOI Creative Commons

Michelle L. Claxton,

Lauren K. Hudson, Daniel W. Bryan

et al.

Microbiology Spectrum, Journal Year: 2022, Volume and Issue: 11(1)

Published: Dec. 15, 2022

Listeria monocytogenes, a foodborne pathogen, and other spp. are present in natural environments. Isolating characterizing strains from reservoirs can provide insight into the prevalence diversity of these environments, elucidate their contribution to contamination agricultural food processing environments products, lead discovery novel species. In this study, we evaluated isolated soil small region Great Smoky Mountains National Park, most biodiverse national park U.S. Park system. Of 17 isolates recovered, whole-genome sequencing revealed that 14 were distinct strains. The represented species (L. monocytogenes [n = 9], L. cossartiae subsp. 1], marthii booriae potentially sp. 2]), as well sequence types based on multilocus typing (MLST) core genome MLST, including many designations. not closely related (≥99.99% average nucleotide identity) any public databases (NCBI, PATRIC), which also indicated novelty. samples study collected high-elevation sites near creek ultimately leads Mississippi River; thus, environment could travel downstream large includes portions nine southeastern midwestern states. This provides indicates is reservoir IMPORTANCE pathogen cause serious systemic illness that, although rare, usually results hospitalization has relatively high mortality rate compared pathogens. Identification diverse insights genomic evolution, ecology, evolution variance pathogenicity genus, especially Comparing such those recovered and/or outbreak may more information about original sources pathways mechanisms products or

Language: Английский

Citations

3