Investigação genômica de patógenos bacterianos de prioridade crítica isolados de pacientes com COVID-19 DOI Creative Commons

J Silva

Published: July 28, 2023

Enterobacter bugandensis (1 isolado, 0,65%), Burkholderia cepacia (13 isolados, 8,45%), Proteus mirabilis Klebsiella variicola (2 1,30%), Escherichia coli Serratia marcescens (5 3,25%), Morganella morganii 1,30%) e Elizabethkingia miricola 0,65%).Especificamente, 17 cepas (11,5%), incluindo K. pneumoniae (7 isolados), P. aeruginosa A. baumannii (4 isolados) E. isolado), apresentaram um perfil de resistência a carbapenêmicos e/ou cefalosporinas amplo espectro, como determinado pelo método disco-difusão.Essas foram submetidas à sequenciamento pela plataforma Illumina NextSeq.A análise genômica revelou resistoma para beta-lactâmicos (blaKPC-2, blaCTX-M-14, blaCTX-M-15), aminoglicosídeos (oqxA, oqxB, qnrE1 aac (6') -Ib-cr) quinolonas (gyrA-83I parC-80I) Adicionalmente, identificados clones internacionais alto risco, ST11, ST16, ST17 ST437, ST244 ST671, ST79 ST730, ST1193.Genes exoU toxA, relacionados com alta virulência o sistema secreção tipo III, em uma cepa aeruginosa, enquanto genes responsáveis produção dos sideróforos enterobactina (ent) aerobactina (iuc/iut) detectados baumannii.Uma limitação do presente estudo é ausência dados clínicos pacientes.Nossos resultados sugerem que pacientes COVID-19 são suscetíveis serem colonizados adquirir infecções secundárias por endêmicos hospitais brasileiros.Essa condição pode contribuir prognóstico desfavorável da infecção COVID-19.

Analysis of the Association Between Antimicrobial Resistance Genes and Virulence Factors in ST11 and Non-ST11 CR-KP Bloodstream Infections in the Intensive Care Unit DOI Creative Commons
Yanye Tu, Hui Gao,

Rongqing Zhao

et al.

Infection and Drug Resistance, Journal Year: 2024, Volume and Issue: Volume 17, P. 4011 - 4022

Published: Sept. 1, 2024

This study aims to investigate the association between antimicrobial resistance genes and virulence factors in ST11 non-ST11 types of CR-KP bloodstream infections intensive care unit, providing a theoretical basis for infection control clinical diagnosis treatment.

Language: Английский

Citations

1
Genomic study substantiates the intensive care unit as a reservoir for carbapenem-resistant Klebsiella pneumoniae in a teaching hospital in China DOI Creative Commons
Shuo Jiang, Zheng Ma, Huiluo Cao

et al.

Microbial Genomics, Journal Year: 2024, Volume and Issue: 10(9)

Published: Sept. 26, 2024

Carbapenem-resistant Klebsiella pneumoniae (CRKP) has recently emerged as a notable public health concern, while the underlying drivers of CRKP transmission among patients across different healthcare facilities have not been fully elucidated. To explore dynamics CRKP, 45 isolates were collected from both intensive care unit (ICU) and non-ICU in teaching hospital Guangdong, China, March 2020 to August 2023. The collection clinical data antimicrobial resistance phenotypes was conducted, followed by genomic analysis for these isolates. mean age 75.2 years, with 18 (40.0%) admitted ICU. predominant strain hospital-acquired sequence type 11 (ST11), k-locus 64 serotype O1/O2v1 (KL64:O1/O2v1), accounting 95.6% (43/45) cases. ST11 ICU exhibited low single nucleotide polymorphism (SNP) distance when compared other departments. Genome-wide association studies identified 17 genes strongly associated SNPs that distinguish those Temporal revealed all departments genetically very close ICU, fewer than 16 SNP differences. further elucidate routes within hospital, we reconstructed detailed patient-to-patient pathways using hybrid methods combine TransPhylo an SNP-based algorithm. A clear route, along mutations potential key genes, deduced coupled information this study, providing insights into settings.

Language: Английский

Citations

1
Hybrid Sequencing-Based Genomic Analysis of Klebsiella pneumoniae from Urinary Tract Infections Among Inpatients at a Tertiary Hospital in Beijing DOI Creative Commons
Zhang We, Yufei Wang, Kaiying Wang

et al.

Infection and Drug Resistance, Journal Year: 2024, Volume and Issue: Volume 17, P. 1447 - 1457

Published: April 1, 2024

Background: Urinary tract infection (UTI) associated with Klebsiella pneumoniae poses a serious threat for inpatients.This study aimed to describe the genomic characteristics of K. causing UTI in tertiary-care hospital Beijing, China.Methods: A total 20 strains collected from 2020 2021 were performed whole-genome sequencing.The Antibiotic susceptibility 19 common antimicrobial agents was tested against all strains.The multi-locus sequence types (MLSTs) and serotypes determined WGS data.De novo assemblies used identify resistance virulence genes.The presence plasmids detected using hybrid assembly long short-read data.Results: These clustered into nine (STs) twelve K-serotypes.All carbapenemresistant (CRKP) acquired carbapenemase bla KPC-2 (n=7).Two CRKP exhibited increased Polymyxin B MIC ≥ 4 mg/L due insertion an IS5-like mgrB gene, they also involved transmission event Intensive Care Unit.Long-read identified many co-carrying multiple replicons.Acquisition new IncM2_1 type CTX-M-3 positive plasmid observed after transfer ICU neurovascular surgery by comparing two same patient.Conclusion: is significant pathogen responsible urinary infections.The ST11-KL47 strain, prevalent at our hospital, exhibits combination high drug hypervirulence.It imperative enhance ongoing surveillance infection-causing pathogens.

Language: Английский

Citations

0
The prevalence of carbapenem-resistant Enterobacterales and the emergence of novel ST11-KL30 carbapenem-resistant Klebsiella pneumoniae in Xinjiang, China DOI Creative Commons
Feng Zhao,

X T Hou,

Gang Sun

et al.

Journal of Global Antimicrobial Resistance, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский

Citations

0
Investigação genômica de patógenos bacterianos de prioridade crítica isolados de pacientes com COVID-19 DOI Creative Commons

J Silva

Published: July 28, 2023

Enterobacter bugandensis (1 isolado, 0,65%), Burkholderia cepacia (13 isolados, 8,45%), Proteus mirabilis Klebsiella variicola (2 1,30%), Escherichia coli Serratia marcescens (5 3,25%), Morganella morganii 1,30%) e Elizabethkingia miricola 0,65%).Especificamente, 17 cepas (11,5%), incluindo K. pneumoniae (7 isolados), P. aeruginosa A. baumannii (4 isolados) E. isolado), apresentaram um perfil de resistência a carbapenêmicos e/ou cefalosporinas amplo espectro, como determinado pelo método disco-difusão.Essas foram submetidas à sequenciamento pela plataforma Illumina NextSeq.A análise genômica revelou resistoma para beta-lactâmicos (blaKPC-2, blaCTX-M-14, blaCTX-M-15), aminoglicosídeos (oqxA, oqxB, qnrE1 aac (6') -Ib-cr) quinolonas (gyrA-83I parC-80I) Adicionalmente, identificados clones internacionais alto risco, ST11, ST16, ST17 ST437, ST244 ST671, ST79 ST730, ST1193.Genes exoU toxA, relacionados com alta virulência o sistema secreção tipo III, em uma cepa aeruginosa, enquanto genes responsáveis produção dos sideróforos enterobactina (ent) aerobactina (iuc/iut) detectados baumannii.Uma limitação do presente estudo é ausência dados clínicos pacientes.Nossos resultados sugerem que pacientes COVID-19 são suscetíveis serem colonizados adquirir infecções secundárias por endêmicos hospitais brasileiros.Essa condição pode contribuir prognóstico desfavorável da infecção COVID-19.

Citations

0