Distinct evolution of ST11 KL64 Klebsiella pneumoniae in Taiwan

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 8, 2023

Carbapenem-resistant ST11_KL64 Klebsiella pneumoniae emerged as a significant public health concern in Taiwan, peaking between 2013 and 2015, with the majority of isolates exhibiting OXA-48 sole carbapenemase. In this study, we employed whole-genome sequencing to investigate molecular underpinnings collected from 2021. Phylogenomic analysis revealed notable genetic divergence strains Taiwan those China, suggesting an independent evolutionary trajectory. Our findings indicated that ST11_KL64_Taiwan lineage originated Brazil, recombination events leading integration ICEKp11 27-kb fragment at tRNAASN sites, shaping its unique genomic landscape. To further elucidate sublineage, examined plasmid contents. contrast ST11_KL64_Brazil strains, which predominantly carried blaKPC-2, exhibited acquisition epidemic blaOXA-48-carrying IncL plasmid. Additionally, consistently harbored multi-drug resistance IncC plasmid, along collection gene clusters conferred heavy metals phage shock protein system via various Inc-type plasmids. Although few, there were still rare have evolved into hypervirulent CRKP through horizontal pLVPK variants. Comprehensive characterization high-risk not only sheds light on success but also provides essential data for ongoing surveillance efforts aimed tracking spread evolution across different geographical regions. Understanding is crucial developing effective strategies combat emergence dissemination.

Language: Английский

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A novel KPC-166 in ceftazidime/avibactam resistant ST307 Klebsiella pneumoniae causing an outbreak in intensive care COVID Unit, Italy

Journal of Microbiology Immunology and Infection, Journal Year: 2024, Volume and Issue: 57(3), P. 457 - 469

Published: March 29, 2024

Aim of the study was molecular characterization 21 ceftazidime/avibactam resistant (CZA-R) Klebsiella pneumoniae strains, collected in period October 2021 - March 2022 from an Intensive Care COVID Unit a Northern Italian Hospital. After growth on selective/chromogenic culture media and susceptibility tests assessment, resistance genes content ascertained for all isolates by HybriSpot 12 multiplexing, PCR Whole-Genome Sequencing (WGS). Clonality assessed PFGE MLST according to Pasteur scheme. A SNPs-based phylogenetic tree obtained comparing representative global genomes. The blaKPC gene horizontal transmission evaluated conjugation experiments. blaKPC-166 cloned pCR2.1 vector transformed chemically competent TOP10 cells. Sixteen inpatients resulted positive colonization and/or infection KPC-producing K. (KPC-Kp) strains. CZA-R KPC-Kp showed MDR phenotype; meropenem always retained. All presented novel variant, named blaKPC-166, showing single nucleotide substitution (T811C) compared blaKPC-94; but related blaKPC-2. 18/21 B 1/21 cases, two strains same patient being untypable PFGE. Interestingly, outbreak sustained high-risk clone ST307, although ST22, ST6342, ST6418 ST6811 have also been identified associated KPC-166. Worryingly, could be transferred horizontally and, after cloning, it conferred CZA. This variant confers CZA–resistance carbapenems restoration. As KPC-166 found expressed multiple Kp clones, greater efforts should made prevent further dissemination such clinical settings.

Language: Английский

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Emergence of NDM-producing Enterobacterales infections in companion animals from Argentina

BMC Veterinary Research, Journal Year: 2024, Volume and Issue: 20(1)

Published: May 3, 2024

Abstract Antimicrobial resistance is considered one of the most critical threat for both human and animal health. Recently, reports infection or colonization by carbapenemase-producing Enterobacterales in companion animals had been described. This study report first molecular characterization NDM-producing causing infections from Argentina. Nineteen out 3662 isolates analyzed between October 2021 July 2022 were resistant to carbapenemes VITEK2C disk diffusion method, suspected be carbapenemase-producers. Ten recovered canine nine feline animals. Isolates identified as K. pneumoniae ( n = 9), E. coli 6) cloacae complex 4), all them presented positive synergy among EDTA carbapenems disks, mCIM/eCIM indicative metallo-carbapenemase production also PCR bla NDM gene. variants determined Sanger sequencing method. All 19 β-lactams aminoglycosides but remained susceptible colistin (100%), tigecycline (95%), fosfomycin (84%), nitrofurantoin (63%), minocycline (58%), chloramphenicol (42%), doxycycline (21%), enrofloxacin (5%), ciprofloxacin (5%) trimethoprim/sulfamethoxazole (5%). Almost (17/19) co-harbored CTX-M plus CMY , harbored alone remaining . CTX-M-1/15 CTX-M-2 groups, while only genes. NDM-1 6), NDM-5 2), 1) confirmed. MLST analysis revealed following sequence types species, : ST15 5), ST273 ST11, ST29; ST162 3), ST457, ST224, ST1196; complex: ST171, ST286, ST544 ST61. To best our knowledge, this description cats. Even though different species clones observed, it remarkable finding some major well circulation main carbapenemase. Surveillance pets needed detect spread carbapenem-resistant alert about dissemination these pathogens humans.

Language: Английский

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Population genomic analysis of clinical ST15 Klebsiella pneumoniae strains in China

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 15, 2023

ST15 Klebsiella pneumoniae (Kpn) is a growing public health concern in China and worldwide, yet its genomic evolutionary dynamics this region remain poorly understood. This study comprehensively elucidates the population genomics of Kpn by analyzing 287 publicly available genomes. The proportion genomes increased sharply from 2012 to 2021, 92.3% them were collected Yangtze River Delta (YRD) eastern China. Carbapenemase genes, including OXA-232, KPC-2, NDM, detected 91.6% studied genomes, 69.2% which multidrug resistant (MDR) hypervirulent (hv). Phylogenetic analysis revealed four clades, C1 (KL112, 59.2%), C2 (mainly KL19, 30.7%), C3 (KL48, 0.7%) C4 (KL24, 9.4%). appeared 2007 was OXA-232-producing hv; between 2005 2007, both KPC-2-producing but with different levels virulence. Transmission clustering 86.1% (n = 247) enrolled strains grouped into 55 clusters (2–159 strains) more transmissible than others. Plasmid profiling 88 plasmid (PCs) that highly heterogeneous within clades. 60.2% ( n 53) PCs carrying AMR genes 7 also harbored VFs. NDM OXA-232 distributed across 14, 4 1 PCs, respectively. MDR-hv all carried one two homologous encoding iucABCD rmpA2 genes. Pangenome major coinciding accessory components predominantly located on plasmids. One component, associated encompassed 15 additional while other, linked involved seven provides essential insights evolution high-risk CP-Kpn warrants rigorous monitoring.

Language: Английский

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DecodingKlebsiella pneumoniaein Poultry Chain: Unveiling Genetic Landscape, Antibiotic Resistance, and Biocide Tolerance in Non-Clinical Reservoirs

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 23, 2024

Abstract The rise of antibiotic resistance in the food chain is influenced by use antimicrobial agents, such as antibiotics, metals, and biocides, throughout entire farm-to-fork continuum. Besides, non-clinical reservoirs potentially contribute to transmission critical pathogens multidrug-resistant (MDR) Klebsiella pneumoniae . However, limited knowledge exists about population structure genomic diversity K. circulating conventional poultry production. We conducted a comprehensive characterization across whole chicken production (flocks/environment/meat, 2019-2022), exploring factors beyond like copper quaternary ammonium compounds (QACs). Clonal adaptive features were characterized through cultural, molecular (FT-IR), whole-genome-sequencing (WGS) approaches. All except one flock positive for with significant increase (p < 0.05) from early pre-slaughter stages, most persisting meat batches. Colistin-resistant rates low (4%), while samples carried MDR strains (67%) copper-tolerant isolates (63%; sil + pco clusters; MIC CuSO4 ≥16mM), particularly at pre-slaughter. Benzalkonium chloride consistently exhibited activity (MIC/MBC range=4-64mg/L) diverse representative independently presence/absence genes linked QACs tolerance. A polyclonal population, discriminated FT-IR WGS, included various lineages dispersed chicken’s lifecycle farm (ST29-KL124, ST11-KL106, ST15-KL19, ST1228-KL38), until (ST1-KL19, ST11-KL111, ST6405-KL109, ST6406-CG147-KL111), or over years (ST631-49 KL109, ST6651-KL107, ST6406-CG147-KL111). Notably, some identical those human clinical isolates. WGS also revealed F-type multireplicon plasmids carrying (copper) co-located qacE Δ1± qacF (QACs) disseminated humans. In conclusion, farms their derived are clones enriched metal tolerance genes, exhibiting genetic similarities strains. Further research imperative unravel influencing persistence dissemination within production, contributing improved safety risk management. This study underscores significance understanding interplay between control strategies sources effectively address spread resistance.

Language: Английский

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