Harnessing biological nitrogen fixation in plant leaves

Trends in Plant Science, Journal Year: 2023, Volume and Issue: 28(12), P. 1391 - 1405

Published: June 1, 2023

Language: Английский

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Synergistic interplay of microbial probiotics in rice rhizosphere: A sustainable strategy for bacterial blight management through microbiome engineering

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102568 - 102568

Published: Jan. 1, 2025

Language: Английский

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Endophytic bacterial communities in wild rice (Oryza officinalis) and their plant growth-promoting effects on perennial rice

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 14, 2023

Endophytic bacterial microbiomes of plants contribute to the physiological health host and its adaptive evolution stress tolerance. Wild rice possesses enriched endophytic bacteria diversity, which is a potential resource for sustainable agriculture. Oryza officinalis unique perennial wild species in China with rich genetic resources. However, communities this their plant growth-promoting (PGP) traits remain largely unknown. In study, root, stem, leaf tissues O. were characterized using 16S rRNA gene Illumina sequencing. Culturable endophytes also isolated from PGP traits. The microbiome analysis showed more complex structure powerful function community roots compared those other tissue compartments. Each compartment had specific biomarkers, including Desulfomonile Ruminiclostridium roots; Lactobacillus, Acinetobacter, Cutibacterium Dechloromonas stems; Stenotrophomonas, Chryseobacterium, Achromobacter Methylobacterium leaves. A total 96 strains phosphate solubilization, potassium release, nitrogen fixation, 1-aminocyclopropane-1-carboxylate (ACC) deaminase secretion, siderophore or indole-3-acetic acid (IAA) production officinalis. Among them, 11 identified as Enterobacter mori, E. ludwigii, cloacae, Bacillus amyloliquefaciens, B. siamensis, Pseudomonas rhodesiae Kosakonia oryzae selected inoculation based on IAA These promising effects seedlings. They promoted form strong root system, stimulate biomass accumulation, increase chlorophyll content uptake, could fulfil ecologically cultivation model rice. results provide insights into endosphere application There prospect mining beneficial species, rewild cultivated varieties promote growth.

Language: Английский

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Comparison of plant microbiota in diseased and healthy rice reveals methylobacteria as health signatures with biocontrol capabilities

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 29, 2024

Introduction Rice ( Oryza sativa ) is a staple food worldwide, but its production under constant pressure from both abiotic and biotic stresses, resulting in high use of agrochemicals. The plant microbiome harbours microorganisms that can benefit health provide alternatives to the composition microbiomes depends on many factors (soil composition, age, health) considered primary driver future health. To identify protect against disease, we hypothesised asymptomatic rice plants fields pathogen (i.e., healthy islands among predominantly diseased plants) might harbour microbiota protects them disease. Material Methods We sampled leaf-diseased with disease incidence Cambodia profiled their at leaf, root, rhizosphere levels using 16S V3V4 18S V4 amplicon barcoding sequencing. Results Comparison sequence variants (ASV) samples revealed signatures (significant enrichment or depletion ASV/species/genus level) fields. genera Methylobacterium Methylorubrum were identified taxa several species significantly enriched leaf indicum , komagatae aerolatum rhodinum ). A cultivation approach led isolation bacterial strains these two genera, which further tested as bioinoculants leaves controlled conditions, showing for some significant reduction (up 77%) symptoms induced by Xanthomonas oryzae pv. infection. Discussion validated hypothesis occurrence host specific biocontrol capacities. This strategy could help new microbes potential sustainable production.

Language: Английский

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Assembly and succession of the phyllosphere microbiome and nutrient-cycling genes during plant community development in a glacier foreland

Environment International, Journal Year: 2024, Volume and Issue: 187, P. 108688 - 108688

Published: April 24, 2024

The phyllosphere, particularly the leaf surface of plants, harbors a diverse range microbiomes that play vital role in functioning terrestrial ecosystems. However, our understanding microbial successions and their impact on functional genes during plant community development is limited. In this study, considering core satellite taxa, we characterized phyllosphere microbiome various microhabitats (i.e., litter, moss leaves) across succession low-altitude glacier foreland. Our findings indicate associated ecosystem stability increase community. abundance taxa increased with was primarily governed by deterministic processes. contrast, decreased mainly stochastic (such as C, N, P hydrolysis fixation) leaves generally succession. litter leaves, only subset (e.g., C fixation degradation, mineralization) showed tendency to Ultimately, both collaboratively influenced characteristics nutrient-cycling genes, leading profiles fluctuating These offer valuable insights into plant-microbe interactions development, advancing significance

Language: Английский

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Distribution patterns of fungal community diversity in the dominant tree species Dacrydium pectinatum and Vatica mangachapoi in tropical rainforests

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

ABSTRACT Plant microbial communities are shaped by plant compartments, but the patterns of fungal in aboveground and belowground which environmental factors can affect them, remain unknown. Here, to address this research gap, high-throughput sequencing technology was performed investigate diversity leaves’ roots’ compartments Dacrydium pectinatum Vatica mangachapoi from Hainan Island China. Fungal leaves roots exhibited significant differences. Eurotiomycetes (16.57%) Dothideomycetes (45.57%) were predominantly found leaves, while Agaricomycetes (36.53%) dominated roots. Compared roots, leaf had higher α-diversity. According Mantel test, soil pH mainly influenced main driving for rainfall temperature. The proportion dispersal-limited processes rhizoplane (76.67%) root endosphere (73.81%) greater than that epiphytic (62.38%) endophytic (68.1%), driven ectomycorrhizal fungi with known dispersal limitations. In summary, compositions both endangered tree species differed, partly unique each compartment. Our results provide valuable theoretical practical insights preserving tropical species. IMPORTANCE Understanding assembly across different is a prerequisite harnessing them enhance growth. findings reveal differences community structures between compartments. While primary drivers those primarily mycorrhizal fungi. These demonstrate compartment-specific plant-microbe interactions responses, offering actionable conserving through habitat optimization (e.g., management) corridor preservation. This compartment-aware perspective enhances our ability leverage functions improve resilience trees face climate change.

Language: Английский

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Microbiome Migration from Soil to Leaves in Maize and Rice

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 947 - 947

Published: April 20, 2025

The interactions between plants and microbes are essential for enhancing crop productivity. However, the mechanisms underlying host-specific microbiome migration functional assembly remain poorly understood. In this study, from soil to leaves in rice (Oryza sativa) maize (Zea mays) was analyzed through 16S rRNA sequencing phenotypic assessments. When we used same source grow maize, microbiota traits were specifically enriched by its phyllosphere rhizosphere. This indicated that can selectively assemble microbiomes a shared source. Therefore, 22 strains isolated phyllospheres of construct synthetic microbial community (SynCom). growth inoculated with SynCom, belonging Bacillus compared phyllosphere. Additionally, strain Rhizobium rhizosphere These results suggest plant species influence within their respective compartments. Compared mock inoculation, SynCom inoculation significantly enhanced growth. microbiomes, Achromobacter, which assembled both played role Our findings underscore importance dynamics leveraging plant–microbe sustainable agriculture.

Language: Английский

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Demethylation Inhibitor Fungicides Have a Significantly Detrimental Impact on Population Growth and Composition of Nectar Microbial Communities

Microbial Ecology, Journal Year: 2024, Volume and Issue: 87(1)

Published: Dec. 1, 2024

Demethylation inhibitor (DMI) fungicides are a mainstay of modern agriculture due to their widespread use for crop protection against plant-pathogenic fungi. However, DMI residues can disperse and persist in the environment, potentially affecting non-target Previous research has demonstrated that DMIs other inhibit yeast growth floral nectar microbial communities decrease fungal richness diversity exposed flowers with no apparent effect on bacteria. Nevertheless, population different species inhabitants dynamics these remains understudied. To address issues, this study we created synthetic including yeasts (Metschnikowia reukaufii Metschnikowia pulcherrima) bacteria (Rosenbergiella epipactidis Comamonas sp.) propagated them culture media containing (imazalil, propiconazole, prothioconazole) at doses or fungicide. Our results showed have significant impact some most common by favoring over yeasts. Furthermore, habitat generalists such as M. pulcherrima sp. were more impacted presence than specialists R. epipactidis, especially upon dispersal across patches. Future should determine if patterns observed present hold true microbes explore interaction between limitation fungicide presence, limitation, mechanisms involved community assembly nectar.

Language: Английский

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Bacterial community composition of wheat aboveground compartments correlates with yield during the reproductive phase

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

Plant-associated microbial communities play important roles in agricultural productivity, and their composition has been shown to vary across plant compartments developmental stages. However, the response of within different at stages diverse long-term fertilization treatments, as well linkages with crop yields, remains underexplored. This study analyzed wheat-associated bacterial various soil under three treatments throughout vegetative reproductive phases. The variance community was primarily attributed compartments, followed by belowground (bulk soil, rhizosphere root) exhibited stronger responses than aboveground (stem leaf). responded all stages, it significantly correlated yields during phase, whereas only showed a which point yields. Moreover, this co-occurrence network enhanced complexity, contained an increased number keystone species associated such

Language: Английский

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Divergence and convergence in epiphytic and endophytic phyllosphere bacterial communities of rice landraces

mSphere, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 30, 2024

ABSTRACT Phyllosphere-associated microbes can significantly alter host plant fitness, with distinct functions provided by bacteria inhabiting the epiphytic (external surface) vs endophytic niches (internal leaf tissue). Hence, it is important to understand assembly and stability of these phyllosphere communities, especially in field conditions. Broadly, communities should encounter more environmental fluctuations frequent immigration, whereas microbiota face stronger selection. As a result, we expect greater variability than communities. We analyzed structure four traditionally cultivated rice landraces one commercial variety from northeast India grown for 3 consecutive years, supplemented opportunistic sampling eight other landraces. Epiphytic bacterial shared dominant core genera such as Methylobacterium Sphingomonas . Consistent an overall strong effect, both varied across years Seeds sampled focal landrace did not support vertical transmission bacteria, suggesting that types are assembled anew each generation. Despite points convergence, had composition higher microbial load were rich, diverse, modular, unstable Finally, focused developmental stages showed divergence between two arose primarily at flowering stage. Thus, our results show convergent divergent patterns community rice, identifying key may aid agricultural interventions increase yield. IMPORTANCE Phyllosphere (leaf-associated) impact making crucial how maintained. While many studies have (surface) relatively poor understanding which colonize very niche formed inside tissues. found several landraces, largely colonized same genera, indicating species level highlighting need mechanisms underlying this divergence. Surprisingly, only weakly shaped landrace, much role factors likely vary over time. microbiome-based increasing productivity could perhaps be generalized varieties but would account temporal instability microbiota. Our thus highlight importance data sets ours—with extensive years—for their applications field.

Language: Английский

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