Endophytic Bacterial Communities in Wild Rice (Oryza eichingeri) and Their Effects on Cultivated Rice Growth DOI Creative Commons
Liang Xie, Jinlu Li,

Suqin Xiao

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(12), P. 2961 - 2961

Published: Dec. 12, 2024

Endophytic bacteria play crucial roles in plant pathogen protection and growth. Oryza eichingeri is a unique wild rice species rich with genetic resources. Studies have explored beneficial endophytic investigated the synergistic interaction between microbes rice. However, bacterial community of their growth-promoting (PGP) abilities characteristics remain largely unknown. Here, root, stem, leaf tissues were characterized using metagenomic Illumina 16S rRNA gene sequencing. Additionally, culturable isolated. The analysis showed that, compared to those other tissue compartments, roots had more complex structure enhanced functions, each compartment its own specific biomarkers. A total 94 isolated from eichingeri, among which 80 strains possessed PGP traits including increasing phosphate solubilization, siderophore production, IAA nitrogen fixation. These displayed good effects on cultivated seedlings, promoting formation strong root systems, stimulating biomass accumulation, length height. findings provide insights into composition endosphere potential applications dominant cultivation.

Language: Английский

Phosphate-Solubilizing Bacteria: Advances in Their Physiology, Molecular Mechanisms and Microbial Community Effects DOI Creative Commons
Lin Pan, Baiyan Cai

Microorganisms, Journal Year: 2023, Volume and Issue: 11(12), P. 2904 - 2904

Published: Dec. 1, 2023

Phosphorus is an essential nutrient for all life on earth and has a major impact plant growth crop yield. The forms of phosphorus that can be directly absorbed utilized by plants are mainly HPO42- H2PO4-, which known as usable phosphorus. At present, the total content soils worldwide 400-1000 mg/kg, only 1.00-2.50% plant-available, seriously affects development agriculture, resulting in high level scarcity available Traditional methods applying fertilizer cannot address deficiency problems; they harm environment ore material nonrenewable natural resource. Therefore, it imperative to find alternative environmentally compatible economically viable strategies scarcity. Phosphorus-solubilizing bacteria (PSB) convert insoluble soil into plants, thus improving uptake utilization plants. However, there no clear systematic report mechanism action PSB. this paper summarizes discovery process, species, distribution PSB, focusing physiological mechanisms outlining processes acidolysis, enzymolysis, chelation complexation reactions related genes regulating PSB acidolysis enzymatic well phosphate transport molecular direction its pathway examined. effects structure abundance microbial communities also described, illustrating how interact with microorganisms indirectly increase amount soil. And three perspectives considered further exploring utilizing synergistic multi-omics approach, PSB-related regulatory different levels investigating application fungicide. This aims provide theoretical support providing optimal management elemental future.

Language: Английский

Citations

57
Microbiome Engineering for Sustainable Rice Production: Strategies for Biofertilization, Stress Tolerance, and Climate Resilience DOI Creative Commons

Israt Jahan Misu,

Md. Omar Kayess, Md. Nurealam Siddiqui

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 233 - 233

Published: Jan. 22, 2025

The plant microbiome, found in the rhizosphere, phyllosphere, and endosphere, is essential for nutrient acquisition, stress tolerance, overall health of plants. This review aims to update our knowledge critically discuss diversity functional roles rice as well microbiome engineering strategies enhance biofertilization resilience. Rice hosts various microorganisms that affect cycling, growth promotion, resistance stresses. Microorganisms carry out these functions through nitrogen fixation, phytohormone metabolite production, enhanced solubilization uptake, regulation host gene expression. Recent research on molecular biology has elucidated complex interactions within microbiomes signalling mechanisms establish beneficial microbial communities, which are crucial sustainable production environmental health. Crucial factors successful commercialization agents include soil properties, practical field conditions, genotype. Advances engineering, from traditional inoculants synthetic biology, optimize availability resilience abiotic stresses like drought. Climate change intensifies challenges, but innovations microbiome-shaping genes (M genes) offer promising solutions crop also discusses agronomic implications emphasizing need further exploration M breeding disease traits. Ultimately, we provide an current findings rice, highlighting pathways productivity sustainably while minimizing impacts.

Language: Английский

Citations

2
Endophytic bacteria for Cd remediation in rice: Unraveling the Cd tolerance mechanisms of Cupriavidus metallidurans CML2 DOI Creative Commons
Yuan Zhang,

Qi Zhou,

Chang Gao

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 469, P. 133846 - 133846

Published: Feb. 23, 2024

The utility of endophytic bacteria in Cadmium (Cd) remediation has gained significant attention due to their ability alleviate metal-induced stress and enhance plant growth. Here, we investigate C. metallidurans CML2, an bacterial strain prevalent rice, showing resilience against 2400 mg/L Cd(II). We conducted in-depth integrated morphological transcriptomic analysis illustrating the multifarious mechanisms CML2 employs combat Cd, including formation biofilm CdO nanoparticles, upregulation genes involved periplasmic immobilization, utilization RND efflux pumps extract excess Cd ions. Beyond exhibited robust tolerance array heavy metals, Cd2+, Mn2+, Se4+, Ni2+, Cu2+, Hg2+, demonstrating effective Cd(II) removal capacity. Furthermore, growth-promoting properties through production indole-3-acetic acid (IAA) at 0.93 mg/L, soluble phosphorus compounds 1.11 siderophores 22.67%. Supportively, pot experiments indicated increase root lengths a decrease bioaccumulation rice seedlings inoculated with consequently reducing translocation rates from 43% 31%. These findings not only contribute understanding resistance but also underscore CML2's promising application within farming ecosystems. Rice Cupriavidus tolerate high cadmium levels (2400 mg/L) its inherent defense make it potential candidate for bio-remediation agriculture. When applied cultivation, reduces soil toxicity crops promotes Consequently, contributes safer food supply, bolsters ecological sustainability, offers viable alternative chemical practices, public health.

Language: Английский

Citations

14
Biocontrol efficacy of Bacillus licheniformis and Bacillus amyloliquefaciens against rice pathogens DOI Creative Commons
Mohsin Tariq, Mehvish Zahoor, Tahira Yasmeen

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18920 - e18920

Published: Jan. 29, 2025

Biocontrol is a cost-effective and eco-friendly approach to control plant pathogens using natural enemies. Antagonistic microorganisms or their derivatives specifically target the while minimizing harm non-target organisms. Bacterial blight brown spot are major rice diseases caused by Xanthomonas oryzae pv. ( Xoo ) Bipolaris Bo ), respectively. This study was conducted assess growth-promoting potential biocontrol activity of root-associated bacteria against pathogens, . A total 98 were isolated from roots characterized for properties including phosphate solubilization, indole-3-acetic acid production, nitrogen fixation biofilm formation. Based on these properties, 36 selected tested via co-culturing antagonism assay. LE7 exhibited maximum inhibition 79%, FR8, PE2, LE7, LR22 LR28 also significantly reduced growth Likewise, FR2, LR22, LR35 inhibited , in which 81%. Under controlled-conditions, disease incidence respectively, improved rice. Full-length 16S rRNA gene sequencing most bacterial isolates, revealed identity with Bacillus amyloliquefaciens licheniformis Application spp. as agent represents enormous farming. The promising isolates could be used bioinoculants management production sustainable manner.

Language: Английский

Citations

1
Perennial Rice – An Alternative to the 'One-sow, One-harvest' Rice Production: Benefits, Challenges, and Future Prospects DOI Creative Commons
S Vijayakumar,

Vikas C. Tyagi,

R. Gobinath

et al.

Farming System, Journal Year: 2025, Volume and Issue: unknown, P. 100137 - 100137

Published: Jan. 1, 2025

Language: Английский

Citations

0
Phytochrome-mediated shade avoidance responses impact the structure and composition of the bacterial phyllosphere microbiome of Arabidopsis DOI Creative Commons

James A. O’Rourke,

Stacey A. Vincent,

I. Ellis Williams

et al.

Environmental Microbiome, Journal Year: 2025, Volume and Issue: 20(1)

Published: Feb. 6, 2025

Abstract The shade avoidance response triggers a dramatic promotion of elongation growth, accompanied by significant reprogramming metabolic pathways as plants seek to prevent overtopping and adapt vegetative shade. Here we demonstrate that simulated results in changes the structure composition phyllosphere bacterial microbiome. Our study uncovered shifts diversity, occurrence, abundance activity bacteria within A comparison responses both wild-type phytochrome mutants, which inherently exhibit shade-avoidance phenotype, revealed indirect host plant physiology direct light among microbiota. Hierarchical clustering patterns further suggested over third taxa constituting core microbiome our assay show some degree Bacteria increased on with phenotype corresponded genera associated beneficial traits such enhanced disease resistance growth promotion. findings suggests manipulate their under conditions strategy optimise fitness when competing for light. We discuss implications terms furthering understanding plant-microbe signalling shaping possibility manipulating health an agricultural setting at high planting densities.

Language: Английский

Citations

0
Wild rice species from Pantanal are hotspots for associations with plant growth-promoting rhizobacteria DOI Creative Commons

Analice Paula de Sousa Campelo,

José Ivo Baldani, Gecele Matos Paggi

et al.

Journal of Plant Interactions, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 2, 2025

Language: Английский

Citations

0
Biological control of Magnaporthe oryzae using natively isolated Bacillus subtilis G5 from Oryza officinalis roots DOI Creative Commons

Ling-Yun Lei,

Zi-Xuan Xiong,

Jinlu Li

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 9, 2023

Rice blast, caused by Magnaporthe oryzae, is a major threat to global rice production causing significant crop losses and impacting grain quality. The annual loss of due this disease ranges from 10% 30%. use biologically controlled strains, instead chemical pesticides, control plant diseases has become research hotspot. In study, an antagonistic endophytic bacterial strain was isolated the roots Oryza officinalis using traditional isolation culture methods. A phylogenetic tree based on 16S RNA whole-genome sequencing identified isolate G5 as Bacillus subtilis. This displayed strong effects against different physiological strains M. oryzae. After co-culture in LB medium for 7 days, inhibition rates mycelial growth four ZB15, WH97, Guy11, T-39800E were 98.07 ± 0.0034%, 98.59 0.0051%, 99.16 0.0012%, 98.69 0.0065%, respectively. Isolate significantly inhibited formation conidia with rate 97% at OD600 2. able provide 66.81% protection blast under potted conditions. Whole-genome revealed that genome size 4,065,878 bp, including 4,182 coding genes. Using anti-SMASH software, 14 secondary metabolite synthesis gene clusters predicted encode antifungal substances, such fengycin, surfactin, bacilysin. also contained genes related promotion. These findings theoretical basis expounding biocontrol mechanisms suggest further development biogenic agents could effectively inhibit pathogen reduce damage, while being environmentally friendly, conducive ecological development, sustainable alternative pesticides. study enriches relevant endophytes wild rice, which proves valuable microbial resource bank.

Language: Английский

Citations

8
Uncovering the secret weapons of an invasive plant: The endophytic microbes of Anthemis cotula DOI Creative Commons
Iqra Bashir,

Aadil Farooq War,

Iflah Rafiq

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(9), P. e29778 - e29778

Published: April 19, 2024

Understanding plant-microbe interaction can be useful in identifying the microbial drivers of plant invasions. It is this context that we explored diversity endophytic microbes from leaves

Language: Английский

Citations

2
Rice sheath blight disease control by native endophytic Bacillus subtilis from Kuttanad, a Globally Important Agricultural Heritage System DOI

Gopika Vijayakumari Krishnan,

Billu Abraham, Ravi S. Lankalapalli

et al.

New Zealand Journal of Botany, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 23

Published: Aug. 22, 2024

Kuttanad in India is known as 'the rice bowl of Kerala' and renowned for its unique cultivation system that below sea level. However, production this wetland ecosystem seriously threatened by sheath blight disease caused the fungus Rhizoctonia solani, which has resulted a significant decline grain quality 50% loss yield. In present study, we isolated two endophytic Bacillus subtilis strains, NIIST B616 B627, from roots plants growing region tested their efficacy controlling disease. We found inoculated with combination both strains R. solani had 64.71% lower incidence 31.02% higher harvest index than treated alone. An analysis defence-related enzymes chemicals revealed treatment isolates together greatly enhanced levels l-phenylalanine ammonia-lyase (PAL; 2.8 fold), peroxidase (POX; 7.4 polyphenol oxidase (PPO; 4.5 total phenol (8.5 fold) compared solely indicating these significantly increased plant defence responses through induced systemic resistance (ISR), finding was validated split-root experiment. More detailed using gas chromatography–tandem mass spectrometry (GC-MS/MS) liquid (LC-MS/MS) techniques cyclo-(Pro-Leu) cyclo-(Pro-Phe) were major components responsible antifungal properties capacity to trigger ISR plants. Thus, metabolites may be deemed viable alternatives prevalence augmenting at locations such Kuttanad.

Language: Английский

Citations

2