Insights into the biocontrol and plant growth promotion functions of Bacillus altitudinis strain KRS010 against Verticillium dahliae

BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 20, 2024

Abstract Background Verticillium wilt, caused by the fungus dahliae , is a soil-borne vascular fungal disease, which has great losses to cotton yield and quality worldwide. The strain KRS010 was isolated from seed of wilt-resistant Gossypium hirsutum cultivar “Zhongzhimian No. 2.” Results broad-spectrum antifungal activity various pathogenic fungi as Botrytis cinerea Fusarium spp., Colletotrichum Magnaporthe oryzae inhibition rate V. mycelial growth 73.97% 84.39% respectively through confrontation test volatile organic compounds (VOCs) treatments. identified Bacillus altitudinis phylogenetic analysis based on complete genome sequences, physio-biochemical characteristics were detected, including growth-promoting ability active enzymes. Moreover, control efficiency against wilt 93.59%. After treatment with culture, biomass reduced. in group (Vd991 alone) 30.76-folds higher than that (KRS010+Vd991). From molecular biological aspect, could trigger plant immunity inducing systemic resistance (ISR) activated salicylic acid (SA) jasmonic (JA) signaling pathways. Its extracellular metabolites VOCs inhibited melanin biosynthesis . In addition, had been characterized promote growth. Conclusions This study indicated B. beneficial microbe potential for controlling cotton, well promoting

Language: Английский

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The potential of Burkholderia gladioli KRS027 in plant growth promotion and biocontrol against Verticillium dahliae revealed by dual transcriptome of pathogen and host

Microbiological Research, Journal Year: 2024, Volume and Issue: 287, P. 127836 - 127836

Published: July 15, 2024

Verticillium dahliae is a destructive, soil-borne pathogen that causes significant losses on numerous important dicots. Recently, beneficial microbes inhabiting the rhizosphere have been exploited and used to control plant diseases. In present study, Burkholderia gladioli KRS027 demonstrated excellent inhibitory effects against wilt in cotton seedlings. Plant growth development was promoted by affecting biosynthesis signaling pathways of brassinosteroids (BRs), gibberellins (GAs), auxins, consequently promoting stem elongation, shoot apical meristem, root tissue division cotton. Furthermore, based host transcriptional response V. infection, it found modulates plants maintain cell homeostasis respond other stress. Moreover, induced disruption cellular structures, as evidenced scanning electron microscopy (SEM) transmission (TEM) analyses. Based comparative transcriptomic analysis between treated group dahliae, substantial alterations transcriptome, particularly genes encoding secreted proteins, small cysteine-rich proteins (SCRPs), protein kinases. addition, suppressed different clonal lineages strains through metabolites, volatile organic compounds (VOCs) released inhibited melanin microsclerotia development. These findings provide valuable insights into an alternative biocontrol strategy for wilt, demonstrating antagonistic bacterium holds promise agent managing disease occurrence.

Language: Английский

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Genome and Transcriptome Analysis to Elucidate the Biocontrol Mechanism of Bacillus amyloliquefaciens XJ5 against Alternaria solani

Microorganisms, Journal Year: 2023, Volume and Issue: 11(8), P. 2055 - 2055

Published: Aug. 10, 2023

Early blight, caused by Alternaria solani, is an important disease affecting tomatoes. Biological control offers environmentally friendly approach to controlling pathogens. Herein, we identified a B. amyloliquefaciens strain XJ5 and investigated its biocontrol mechanism against A. solani. solani growth was significantly inhibited XJ5, with the inhibition rate of cell-free culture supernatants reaching 82.3%. Furthermore, crude protein extracts conidia germination altered mycelial morphology To uncover potential analyzed genome sequence transcriptome. The comprised 4.16 Mb circular chromosome two plasmids. A total 13 biosynthetic gene clusters 127 genes encoding hydrolases were identified, suggestive ability secrete antagonistic secondary metabolites hydrolases. Transcript analysis revealed 174 differentially expressed on exposing extracts. expression related chitin mannose synthesis downregulated, indicating that may impact in Overall, these findings enhance our understanding interactions between phytopathogens pave way for agricultural application this promising agent.

Language: Английский

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Antibiotic‐producing plant‐associated bacteria, anti‐virulence therapy and microbiome engineering: Integrated approaches in sustainable agriculture

Microbial Biotechnology, Journal Year: 2024, Volume and Issue: 17(10)

Published: Oct. 1, 2024

Abstract Plant health is crucial for maintaining the well‐being of humans, animals and environment. pathogens pose significant challenges to agricultural production, global food security ecosystem biodiversity. This problem exacerbated by impact climate change, which expected alter emergence evolution plant their interaction with hosts. Traditional approaches managing phytopathogens involved use chemical pesticides, but alternative strategies are needed address ongoing decline in performance as well negative on environment public health. Here, we highlight advancement effectiveness biocontrol based antimicrobial‐producing plant‐associated bacteria, anti‐virulence therapy (e.g. quorum quenching) microbiome engineering sustainable biotechnological promote foster agriculture. Notably, Enterobacterales emerging important agents a source new antimicrobials potential use. We analysed here genomes over 250 enterobacteria examine synthesize secondary metabolites. Exploration major interest search eco‐friendly alternatives reducing pesticides.

Language: Английский

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Unlocking antagonistic potential of Bacillus amyloliquefaciens KRS005 to control gray mold

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: June 2, 2023

To establish a safe, efficient, and simple biocontrol measure for gray mold disease caused by Botrytis cinerea, the basic characteristics antifungal activity of KRS005 were studied from multiple aspects including morphological observation, multilocus sequence analysis typing (MLSA-MLST), physical-biochemical assays, broad-spectrum inhibitory activities, control efficiency mold, determination plant immunity. The strain KRS005, identified as Bacillus amyloliquefaciens, demonstrated activities against various pathogenic fungi dual confrontation culture which inhibition rate B. cinerea was up to 90.3%. Notably, through evaluation efficiency, it found that fermentation broth could effectively occurrence tobacco leaves determining lesion diameter biomass on still had high effect after dilution 100 folds. Meanwhile, no impact mesophyll tissue leaves. Further studies showed defense-related genes involved in reactive oxygen species (ROS), salicylic acid (SA), jasmonic (JA)-related signal pathways significantly upregulated when sprayed with cell-free supernatant. In addition, inhibit cell membrane damage increase permeability cinerea. Overall, promising agent, would likely serve an alternative chemical fungicides mold.

Language: Английский

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Microbiota and the plant immune system work together to defend against pathogens

Archives of Microbiology, Journal Year: 2023, Volume and Issue: 205(10)

Published: Oct. 1, 2023

Language: Английский

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Pesticides and winemaking: A comprehensive review of conventional and emerging approaches

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2024, Volume and Issue: 23(5)

Published: Aug. 8, 2024

Abstract The use of pesticides in viticulture may play a crucial role ensuring the health and quality grapes. This review analyzes most common used, illustrating their classification toxicity, variations throughout winemaking process. Fungicides are generally harmless or mildly toxic, whereas insecticides classified as either highly moderately hazardous. Potential alternatives to synthetic wine production also reviewed, thereby including biopesticides biological agents. Analytical methods for detecting quantifying pesticide residues then described, liquid chromatography gas coupled with mass spectrometry. discusses impact process on content. Pesticides strong hydrophobicity were more likely accumulate solid byproducts, hydrophilic distributed phase. Grape's skin contains lipids, so hydrophobic adsorb strongly grape surfaces clarification has been shown be effective reduction compounds. Therefore, final could have quantities pesticides. Alcoholic fermentation dissipation. However, an antagonistic effect yeasts, affecting safety products. proteomic genomic analyses yeast growth reviewed understand effects during fermentation. last section describes new used removing from grapes wine, improving product reducing harmful effects.

Language: Английский

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Biocontrol of Colletotrichum fructicola in the Postharvest Banana Fruit Using the Siderophore-Producing Strain BX1

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(40), P. 22132 - 22143

Published: Sept. 24, 2024

Banana anthracnose, caused by

Language: Английский

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A Novel Strain Burkholderia theae GS2Y Exhibits Strong Biocontrol Potential Against Fungal Diseases in Tea Plants (Camellia sinensis)

Cells, Journal Year: 2024, Volume and Issue: 13(21), P. 1768 - 1768

Published: Oct. 25, 2024

Tea plants (

Language: Английский

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Bioactive Compounds/Metabolites from Extremophiles for Biocontrol and Plant Disease Management

Published: Jan. 1, 2024

Language: Английский

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