Bacillus-based biocontrol beyond chemical control in central Africa: the challenge of turning myth into reality

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 6, 2024

Agricultural productivity in the Great Lakes Countries of Central Africa, including Burundi, Rwanda, and Democratic Republic Congo, is affected by a wide range diseases pests which are mainly controlled chemical pesticides. However, more than 30% pesticides used region banned European Union due to their high toxicity. Globally available safe eco-friendly biological alternatives chemicals virtually non-existent region. Bacillus PGPR-based biocontrol products most dominant market have proven efficacy controlling major plant reported With this review, we present current situation disease pest management urge need utilize -based control as possible sustainable alternative A repertoire strains from subtilis group that shown great potential antagonize local pathogens provided, efforts promote use, well search for indigenous adapted agro-ecological conditions, should be undertaken make agriculture reality

Language: Английский

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Bacillus velezensis YXDHD1-7 Prevents Early Blight Disease by Promoting Growth and Enhancing Defense Enzyme Activities in Tomato Plants

Microorganisms, Journal Year: 2024, Volume and Issue: 12(5), P. 921 - 921

Published: April 30, 2024

Bacillus velezensis is well known as a plant growth-promoting rhizobacteria (PGPR) and biocontrol agent. Nevertheless, there are very few reports on the study of B. tomato early blight, especially effects among different inoculation concentrations. In this study, an IAA-producing strain, YXDHD1-7 was isolated from rhizosphere soil, which had strongest inhibitory effect against Alternaria solani. Inoculation with bacterial suspensions strain promoted growth seedlings effectively. Furthermore, inoculations at 106, 107, 108 cfu/mL resulted in control efficacies 100%, 83.15%, 69.90%, respectively. Genome sequencing showed that it possesses 22 gene clusters associated synthesis antimicrobial metabolites genes involved production IAA. may be able to produce spermidine volatile compounds also enhance defense responses. Our results suggest prevents blight disease by promoting enhancing enzyme activities plants. This promising candidate for excellent microbial inoculant can used production.

Language: Английский

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Bacillus cabrialesii subsp. cabrialesii Strain TE5: A Promising Biological Control Bacterium against the Causal Agent of Spot Blotch in Wheat

Plants, Journal Year: 2025, Volume and Issue: 14(2), P. 209 - 209

Published: Jan. 13, 2025

Strain TE5 was isolated from a wheat (Triticum turgidum L. subsp. durum) rhizosphere grown in commercial field of the Yaqui Valley Mexico. In this work, we present strain as promising biological control agent against Bipolaris sorokiniana. First, after its genome sequencing through Illumina NovaSeq, showed size 4,262,927 bp, with 43.74% G + C content, an N50 value 397,059 L50 4 and 41 contigs (>500 bp). Taxonomical affiliation carried out by using overall relatedness indexes (OGRIs) construction phylogenomic tree based on whole genome. The results indicated that identifies Bacillus cabrialesii cabrialesii. Genomic annotation Rapid Annotation Using Subsystems Technology (RAST) Prokaryotic Genome (Prokka) presence 4615 coding DNA sequences (CDSs) distributed across 330 subsystems, which included gene families associated biocontrol, stress response, iron competition. Furthermore, when antiSMASH 7.1 platform used for mining, seven putative biosynthetic clusters related to production biocontrol metabolites, namely subtilosin A, bacillibactin, fengycin, bacillaene, bacilysin, surfactin, rhizocticin A. Moreover, antifungal activity cell-free extract (CFE) evaluated sorokiniana, emergent pathogen. vitro dual confrontation fungal growth inhibition 67% TE5. Additionally, CFE almost completely inhibited (93%) studied phytopathogenic fungus liquid media. Further observations impact these bacterial metabolites spore germination exhibited spores degrading germinative hypha, avoiding mycelium development. Finally, protective effect sorokiniana seedlings. significant decrease (83%) disease severity comparison plant infection without inoculation agent. Thus, work proposes pathogen while suggesting lipopeptides potential mode action, together defense stimulation.

Language: Английский

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Genomic and functional characterization of the Pantoea ananatis nfd35 strain, a potential biocontrol agent of anthracnose disease in Trichosanthes kirilowii Maxim

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102683 - 102683

Published: April 1, 2025

Language: Английский

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Molecular techniques for the improvement of microbial biocontrol agents against plant pathogens

Egyptian Journal of Biological Pest Control, Journal Year: 2023, Volume and Issue: 33(1)

Published: Oct. 12, 2023

Abstract Biological control is a sustainable and ecologically effective method for bringing down pest population to an acceptable level. Implementing microbial biocontrol agents (MBCAs) manage plant diseases necessitates the use of environmentally friendly practices that can increase global food production guarantee safety supply. Recent advancements in biotechnology have made it easier find characterize new beneficial microbes as well identify their genetic byproducts. These findings possible clone these plants effort strengthen resistance biotic abiotic stresses. The technological improvements strengthened symbiotic interaction between while also enabling modification processes through which MBCAs exert effects, ultimately enhancing potential managing diseases. genome sequencing has yielded useful information about genomes, helped them efficiently. This article offers thorough summary already existing recent molecular advances used efficiency understand mechanisms various omics technologies. approaches are important assuring security increasing agricultural outputs by minimizing yield loss due

Language: Английский

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Deciphering the mechanisms involved in reduced sensitivity to azoles and fengycin lipopeptide in Venturia inaequalis

Microbiological Research, Journal Year: 2024, Volume and Issue: 286, P. 127816 - 127816

Published: June 27, 2024

Apple scab, caused by the hemibiotrophic fungus Venturia inaequalis, is currently most common and damaging disease in apple orchards. Two strains of V. inaequalis (S755 Rs552) with different sensitivities to azole fungicides bacterial metabolite fengycin were compared determine mechanisms responsible for these differences. Antifungal activity tests showed that Rs552 had reduced sensitivity tebuconazole tetraconazole, as well alone or a binary mixture other lipopeptides (iturin A, pumilacidin, lichenysin). S755 was highly sensitive fengycin, whose close tebuconazole. Unlike from iturin family (mycosubtilin, A) similar on both strains, while those surfactin (lichenysin, pumilacidin) not active, except mixtures fengycin. The varies according their structure. Analyses difference azoles (which target CYP51 enzyme involved ergosterol biosynthesis pathway) linked (i) constitutive increased expression Cyp51A gene insertions upstream region (ii) greater efflux membrane pumps involvement ABC transporters. Microscopic observations revealed known interact plasma membranes, induced morphological cytological changes cells strains. Sterol phospholipid analyses higher level ergosta-7,22-dien-3-ol lower PI(C16:0/C18:1) S755. These differences could therefore influence composition explain differential However, antifungal activities mycosubtilin A two indirectly indicate sterols are probably resistance mechanism. This leads conclusion susceptibility studied.

Language: Английский

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Characterization of a Bacterium Isolated from Hydrolyzed Instant Sea Cucumber Apostichopus japonicus Using Whole-Genome Sequencing and Metabolomics

Foods, Journal Year: 2024, Volume and Issue: 13(22), P. 3662 - 3662

Published: Nov. 17, 2024

Autolysis in the sea cucumber

Language: Английский

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Genomic Insights into the Bactericidal and Fungicidal Potential of Bacillus mycoides b12.3 Isolated in the Soil of Olkhon Island in Lake Baikal, Russia

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2450 - 2450

Published: Nov. 28, 2024

The dispersal of plant pathogens is a threat to the global economy and food industry which necessitates need discover efficient biocontrol agents such as bacteria, fungi, etc., inhibiting them. Here, we describe Bacillus mycoides strain b12.3 isolated from soil Olkhon Island in Lake Baikal, Russia. By applying co-cultivation technique, found that inhibits growth pathogens, bacteria Xanthomonas campestris, Clavibacter michiganensis, Pectobacterium atrospecticum, well fungus Alternaria solani. To elucidate genomic fundament explaining these activities, leveraged next-generation whole-genome sequencing obtained high-quality assembly based on short reads. isolate bore seven known BGCs (biosynthetic gene clusters), including those responsible for producing bacillibactin, fengycin, petrobactin. Moreover, genome contained insecticidal genes encoding App4Aa1, Tpp78Ba1, Spp1Aa1 toxins, thus implicating possible pesticidal potential. We compared with 50 closest assemblies enriched BGCs. analysis also revealed architecture corresponds experimentally observed activity spectrum implying combination produced secondary metabolites delineates range inhibited phytopathogens Therefore, this study deepens our knowledge biology ecology B. residing Baikal region.

Language: Английский

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Pathogen-Activated Chaetomium Globosum G3 Enhances Iron Competition and Other Antagonistic Mechanisms to Suppress Maize Seedling Blight Causal Agent Fusarium Verticillioides

Published: Jan. 1, 2024

Language: Английский

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Deciphering the Mechanisms Involved in Reduced Sensitivity to Azoles and Fengycin Lipopeptide in Venturia Inaequalis

Published: Jan. 1, 2024

Language: Английский

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