Synthesis, Assembly, and Applications of Hybrid Nanostructures for Biosensing DOI
Shuaidi Zhang,

Ren Geryak,

Jeffrey A. Geldmeier

et al.

Chemical Reviews, Journal Year: 2017, Volume and Issue: 117(20), P. 12942 - 13038

Published: Sept. 13, 2017

The robust, sensitive, and selective detection of targeted biomolecules in their native environment by prospective nanostructures holds much promise for real-time, accurate, high throughput biosensing. However, order to be competitive, current biosensor nanotechnologies need significant improvements, especially specificity, integration, rate, long-term stability complex bioenvironments. Advancing biosensing chemically "noisy" bioenvironments require careful engineering nanoscale components that are highly biorecognition ligands capable exquisite binding, seamless integration at a level devices have yet achieve. This review summarizes recent advances the synthesis, assembly, applications nanoengineered reporting transducing critical efficient First, major classes nanostructured components, both inorganic reporters organic transducers, discussed context synthetic control individual compositions, shapes, properties. Second, design surface functionalities path, characterization interfacial architectures, multiple into multifunctional ordered extensively examined. Third, examples structures created from hybrid nanomaterials reviewed, with distinct emphasis on tailor nanosensor designs specific operating environments. Finally, we offer perspective future developments nanohybrid materials nanosensors, outline possible directions pursued may yield breakthrough results, envision exciting potential high-performance will cause disruptive improvements field

Language: Английский

Super-resolution microscopy demystified DOI
Lothar Schermelleh, Alexia Ferrand, Thomas Huser

et al.

Nature Cell Biology, Journal Year: 2018, Volume and Issue: 21(1), P. 72 - 84

Published: Dec. 17, 2018

Language: Английский

Citations

1007
Colloidal nanoparticles as advanced biological sensors DOI
Philip D. Howes, Rona Chandrawati, Molly M. Stevens

et al.

Science, Journal Year: 2014, Volume and Issue: 346(6205)

Published: Oct. 2, 2014

Colloidal nanoparticle biosensors have received intense scientific attention and offer promising applications in both research medicine. We review the state of art development, surface chemistry, biosensing mechanisms, discussing how a range technologies are contributing toward commercial clinical translation. Recent examples success include ultrasensitive detection cancer biomarkers human serum vivo sensing methyl mercury. identify five key materials challenges, including development robust mass-scale synthesis methods, broader use simulations bioinformatics-driven experimental approaches for predictive modeling biosensor performance. The resultant generation will form basis high-performance analytical assays, effective multiplexed intracellular sensors, sophisticated probes.

Language: Английский

Citations

942
Is there new hope for therapeutic matrix metalloproteinase inhibition? DOI Open Access
Roosmarijn E. Vandenbroucke, Claude Libert

Nature Reviews Drug Discovery, Journal Year: 2014, Volume and Issue: 13(12), P. 904 - 927

Published: Nov. 7, 2014

Language: Английский

Citations

743
A general protocol for the generation of Nanobodies for structural biology DOI
Els Pardon, Toon Laeremans, Sarah Triest

et al.

Nature Protocols, Journal Year: 2014, Volume and Issue: 9(3), P. 674 - 693

Published: Feb. 27, 2014

Language: Английский

Citations

702
Biosensors for Whole-Cell Bacterial Detection DOI Open Access
Asif Ahmed, Jo V. Rushworth,

Natalie A. Hirst

et al.

Clinical Microbiology Reviews, Journal Year: 2014, Volume and Issue: 27(3), P. 631 - 646

Published: June 30, 2014

Bacterial pathogens are important targets for detection and identification in medicine, food safety, public health, security. infection is a common cause of morbidity mortality worldwide. In spite the availability antibiotics, these infections often misdiagnosed or there an unacceptable delay diagnosis. Current methods bacterial rely upon laboratory-based techniques such as cell culture, microscopic analysis, biochemical assays. These procedures time-consuming costly require specialist equipment trained users. Portable stand-alone biosensors can facilitate rapid diagnosis at point care. Biosensors will be particularly useful where clear informs treatment, critical illness (e.g., meningitis) to prevent further disease spread case food-borne sexually transmitted diseases). Detection bacteria also becoming increasingly antibioterrorism measures anthrax detection). this review, we discuss recent progress use whole cells sensitive earlier without need sample processing. There particular focus on electrochemical biosensors, especially impedance-based systems, present key advantages terms ease miniaturization, lack reagents, sensitivity, low cost.

Language: Английский

Citations

603
The Therapeutic Potential of Nanobodies DOI Creative Commons
Ivana Jovčevska, Serge Muyldermans

BioDrugs, Journal Year: 2019, Volume and Issue: 34(1), P. 11 - 26

Published: Nov. 4, 2019

Today, bio-medical efforts are entering the subcellular level, which is witnessed with fast-developing fields of nanomedicine, nanodiagnostics and nanotherapy in conjunction implementation nanoparticles for disease prevention, diagnosis, therapy follow-up. Nanoparticles or nanocontainers offer advantages including high sensitivity, lower toxicity improved safety—characteristics that especially valued oncology field. Cancer cells develop proliferate complex microenvironments leading to heterogeneous diseases, often a fatal outcome patient. Although antibody-based widely used clinical care patients solid tumours, its efficiency definitely needs improvement. Limitations antibodies result mainly from their big size poor penetration tissues. Nanobodies novel unique class antigen-binding fragments, derived naturally occurring heavy-chain-only present serum camelids. Their superior properties such as small size, stability, strong affinity, water solubility natural origin make them suitable development into next-generation biodrugs. Less than 30 years after discovery functional antibodies, nanobody derivatives already extensively by biotechnology research community. Moreover, number nanobodies under investigation wide spectrum human diseases inflammation, breast cancer, brain lung infectious diseases. Recently, caplacizumab, bivalent nanobody, received approval European Medicines Agency (EMA) US Food Drug Administration (FDA) treatment thrombotic thrombocytopenic purpura.

Language: Английский

Citations

599
Advances and applications of nanophotonic biosensors DOI Open Access
Hatice Altug, Sang‐Hyun Oh, Stefan A. Maier

et al.

Nature Nanotechnology, Journal Year: 2022, Volume and Issue: 17(1), P. 5 - 16

Published: Jan. 1, 2022

Language: Английский

Citations

586
Antibody Structure and Function: The Basis for Engineering Therapeutics DOI Creative Commons
Mark L. Chiu, Dennis R. Goulet, A. Teplyakov

et al.

Antibodies, Journal Year: 2019, Volume and Issue: 8(4), P. 55 - 55

Published: Dec. 3, 2019

Antibodies and antibody-derived macromolecules have established themselves as the mainstay in protein-based therapeutic molecules (biologics). Our knowledge of structure–function relationships antibodies provides a platform for protein engineering that has been exploited to generate wide range biologics host indications. In this review, our basic understanding antibody structure is described along with how leveraged antibody-related therapeutics having appropriate antigen affinity, effector function, biophysical properties. The platforms examined include development antibodies, fragments, bispecific antibody, fusion products, whose efficacy manufacturability can be improved via humanization, affinity modulation, stability enhancement. We also review design selection binding arms, avidity modulation. Different strategies preparing multispecific an array applications are included.

Language: Английский

Citations

491
A robust pipeline for rapid production of versatile nanobody repertoires DOI
Peter C. Fridy, Yinyin Li, Sarah Keegan

et al.

Nature Methods, Journal Year: 2014, Volume and Issue: 11(12), P. 1253 - 1260

Published: Nov. 2, 2014

Language: Английский

Citations

484
Nanobodies and Nanobody-Based Human Heavy Chain Antibodies As Antitumor Therapeutics DOI Creative Commons
Peter Bannas, Julia Hambach, Friedrich Koch‐Nolte

et al.

Frontiers in Immunology, Journal Year: 2017, Volume and Issue: 8

Published: Nov. 22, 2017

Monoclonal antibodies have revolutionized cancer therapy. However, delivery to tumor cells in vivo is hampered by the large size (150 kDa) of conventional antibodies. The minimal target recognition module a antibody composed two non-covalently associated variable domains (VH and VL). proper orientation these mediated their hydrophobic interface stabilized linkage disulfide-linked constant (CH1 CL). VH VL can be fused via genetic linker into single chain fragment (scFv). scFv modules turn one another, e.g. generate bispecific T cell engager (BiTE), or they various orientations hinge Fc bi- multispecific inherent interaction limits stability solubility engineered antibodies, often causing aggregation and/or mispairing V-domains. Nanobodies (15 nanobody-based human heavy (75 overcome limitations. Camelids naturally produce only chains which domain (VHH nanobody). Advantageous features nanobodies include small size, high solubility, stability, excellent tissue penetration vivo. readily linked genetically Fc-domains, other nanobodies, peptide tags, toxins conjugated chemically at specific site drugs, radionuclides, photosensitizers, nanoparticles. These properties make them particularly suited for efficient targeting tumors Chimeric nanobody-heavy combine advantageous about half antibody. In this review, we discuss recent developments perspectives applications as anti-tumor therapeutics.

Language: Английский

Citations

474