Journal of Cell Science,
Journal Year:
2024,
Volume and Issue:
137(20)
Published: Oct. 15, 2024
In
2023,
the
ImaBio
consortium
(imabio-cnrs.fr),
an
interdisciplinary
life
microscopy
research
group
at
Centre
National
de
la
Recherche
Scientifique,
celebrated
its
20th
anniversary.
contributes
to
biological
imaging
community
through
organization
of
MiFoBio
conferences,
which
are
conferences
featuring
lectures
and
hands-on
workshops
that
attract
specialists
from
around
world.
provide
with
opportunity
reflect
on
evolution
field,
2023
event
offered
retrospective
talks
discussing
past
20
years
topics
in
microscopy,
including
multicellular
assemblies,
image
analysis,
quantification
molecular
motions
interactions
within
cells,
advancements
fluorescent
labels,
laser
technology
for
multiphoton
label-free
thick
samples.
this
Perspective,
we
compile
summaries
these
presentations
overviewing
a
specific
area
each
concludes
brief
look
towards
future.
The
full
available
YouTube
channel
(youtube.com/@gdrimabio5724).
Chemical Reviews,
Journal Year:
2024,
Volume and Issue:
124(22), P. 12573 - 12660
Published: Nov. 13, 2024
Cellular
function
is
controlled
through
intricate
networks
of
signals,
which
lead
to
the
myriad
pathways
governing
cell
fate.
Fluorescent
biosensors
have
enabled
study
these
signaling
in
living
systems
across
temporal
and
spatial
scales.
Over
years
there
has
been
an
explosion
number
fluorescent
biosensors,
as
they
become
available
for
numerous
targets,
utilized
spectral
space,
suited
various
imaging
techniques.
To
guide
users
this
extensive
biosensor
landscape,
we
discuss
critical
aspects
proteins
consideration
development,
smart
tagging
strategies,
historical
recent
types,
grouped
by
target,
with
a
focus
on
design
applications
sensors
systems.
Journal of Experimental Botany,
Journal Year:
2024,
Volume and Issue:
75(17), P. 5312 - 5324
Published: Feb. 22, 2024
Abstract
Chloroplasts
fix
carbon
by
using
light
energy
and
have
evolved
a
complex
redox
network
that
supports
plastid
functions
(i)
protecting
against
reactive
oxygen
species
(ii)
metabolic
regulation
in
response
to
environmental
conditions.
In
thioredoxin-
glutathione/glutaredoxin-dependent
cascades,
protein
cysteinyl
steady
states
are
set
varying
oxidation
reduction
rates.
The
specificity
interplay
of
these
different
redox-active
proteins
still
under
investigation,
for
example
understand
how
plants
cope
with
adverse
conditions
acclimation.
Genetically
encoded
biosensors
distinct
can
be
targeted
subcellular
compartments
such
as
the
chloroplast
stroma,
enabling
vivo
real-time
measurements
physiological
parameters
at
scales.
These
data
provided
unique
insights
into
dynamic
behaviours
redox-responsive
several
levels
known
cascades.
This
review
summarizes
current
applications
biosensor
types
well
dynamics
states,
an
emphasis
on
responses.
Trends in Cell Biology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 1, 2025
HighlightsSystems-level
approaches
complement
static
insights
by
revealing
how
gene
regulatory
networks
(GRNs)
dynamically
regulate
cellular
aging
and
promote
longevity.Observing
the
dynamics
of
heme
biosynthesis
nucleolar
silencing
in
budding
yeast
reveals
distinct
modes,
synthetic
biology
can
significantly
extend
lifespan.Negative
feedback
loops
aging-related
processes
such
as
proteostasis,
energy
metabolism,
DNA
damage
response
pathways
maintain
homeostasis
counteract
deterioration.Synthetic
offers
potential
interventions
to
reinforce
circuits,
thereby
mitigating
age-related
diseases
promoting
healthy
aging.AbstractAging
is
a
dynamic
process
that
driven
disruption
homeostatic
(GRNs).
Traditional
studies
often
focus
on
individual
genes,
but
understanding
their
interplay
key
unraveling
mechanisms
aging.
This
review
explores
circuits
influence
longevity
highlights
role
maintaining
balance.
The
SIR2–HAP
circuit
serves
model
explore
mutual
inhibition
between
influences
trajectories
engineering
stable
fixed
points
or
oscillations
within
these
lifespan.
Feedback
crucial
for
are
also
reviewed,
we
highlight
destabilization
accelerates
By
leveraging
systems
biology,
strategies
proposed
may
stabilize
single
cells,
enhancing
resilience
damage.
Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 27, 2025
Understanding
kinase
action
requires
precise
quantitative
measurements
of
their
activity
in
vivo
.
In
addition,
the
ability
to
capture
spatial
information
is
crucial
deconvolute
complex
signaling
networks,
interrogate
multifaceted
actions,
and
assess
drug
effects
or
genetic
perturbations.
Here
we
developed
a
proteomic
sensor
platform
(ProKAS)
for
analysis
using
mass
spectrometry.
ProKAS
based
on
tandem
array
peptide
sensors
with
amino
acid
barcodes
that
allow
multiplexed
spatial,
kinetic,
screening
applications.
We
engineered
module
simultaneously
monitor
activities
DNA
damage
response
kinases
ATR,
ATM,
CHK1
genotoxic
drugs,
while
also
uncovering
differences
between
these
responses
nucleus,
cytosol,
replication
factories.
Furthermore,
an
silico
approach
rational
design
specific
substrate
peptides
expandable
other
kinases.
Overall,
novel
versatile
system
systematically
spatially
probing
cells.
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: April 25, 2025
Coenzyme
Q10
(Q10)
plays
a
critical
role
in
cellular
energy
conversion
within
the
mitochondrial
respiratory
chain
and
offers
protective
effects
against
oxidative
metabolic
stress.
In
this
study,
we
investigated
impact
of
on
spatio-temporal
patterns
energetics
keratinocyte-derived
HaCaT
cells,
utilizing
genetically-encoded
FRET
sensor
AMPfret.
Engineered
from
AMP-activated
protein
kinase
(AMPK),
leverages
endogenous
affinities
that
evolved
to
detect
stress,
specifically
decreases
ATP/ADP
ATP/AMP
ratios
pose
threat
cell
survival.
We
successfully
established
cells
stably
expressing
AMPfret,
validated
their
functionality
by
inducing
stress
with
2-deoxy-D-glucose,
demonstrated
Q10,
together
high
glucose
conditions
culture,
can
enhance
compared
low
controls.
then
employed
AMPfret
analyze
response
keratinocytes
Luperox
(tert-butyl
peroxide),
potent
organic
prooxidant,
presence
varying
intracellular
levels
Q10.
Preloading
was
protective,
slowing
speed
reducing
extend
response.
contrast,
preincubation
Simvastatin,
an
inhibitor
mevalonate
biosynthesis
pathway,
depleted
levels,
accelerated
onset
led
early
death
as
Under
all
conditions,
revealed
cell-to-cell
heterogeneity
at
baseline
Luperox.
Overall,
tracking
changes
state
time
single-cell
level
allows
further
insights
into
beneficial
enhancing
bioenergetics
skin
potential
AMPK
mediating
responses
altered
levels.
