Plant microtubule nucleating apparatus and its potential signaling pathway DOI Creative Commons
Noriyoshi Yagi, Satoshi Fujita, Masayoshi Nakamura

et al.

Current Opinion in Plant Biology, Journal Year: 2024, Volume and Issue: 82, P. 102624 - 102624

Published: Sept. 3, 2024

Plant cell cortical microtubules are located beneath the plasma membrane and direct location of cellulose synthases during interphase, influencing morphology. Microtubule-associated proteins (MAPs) regulate these in response to growth environmental stimuli. This review focuses on recent advances understanding microtubule nucleation mechanisms plants spatiotemporal regulation arrays via phytohormone signaling. Emphasis is placed conserved nature gamma-tubulin ring complex (γTuRC) plant-specific components. The discussion includes role Augmin distinct function Msd1-Wdr8 plants. We also explore effects hormone signaling, particularly brassinosteroids, regulatory apparatus. interplay between signaling pathways dynamics, including phosphorylation events post-translational modifications, addressed. Finally, impact signals protein modifications regulating organization suggested for future research.

Language: Английский

Optical Control of Microtubule Accumulation and Dispersion by Tau-Derived Peptide-Fused Photoresponsive Protein DOI Creative Commons

Soei Watari,

Hiroshi Inaba,

Qianru H. Lv

et al.

JACS Au, Journal Year: 2025, Volume and Issue: 5(2), P. 791 - 801

Published: Jan. 23, 2025

Microtubules, a major component of the cytoskeleton consisting tubulin dimers, are involved in various cellular functions, including forming axons and dendrites neurons retaining cell shapes by accumulated superstructures such as bundles doublets. Moreover, microtubule-accumulated structures like swarming microtubule assemblies attractive components for dynamic materials, active matter molecular robots. Thus, control is an important topic. However, implementing stimulus-dependent remains challenging. This challenge can be resolved developing designer protein approaches. We have previously developed Tau-derived peptide (TP), which binds to inner or outer surface microtubules depending on timing incubation. In this report, we designed TP-fused photoswitchable Dronpa (TP-Dronpa) that reversibly photoconverts between monomeric tetrameric states photocontrol assemblies. The formation superstructures, doublets, was induced TP-Dronpa, whereas TP-Dronpa ensured remained dispersed. Tetrameric also motile aster-like movement kinesin-coated substrate. formation/dissociation these controlled light irradiation. system generate provides approach facilitate assembly materials applications.

Language: Английский

Citations

1
Doublecortin restricts neuronal branching by regulating tubulin polyglutamylation DOI Creative Commons
Muriel Sébastien, Alexandra Paquette, Emily N. P. Prowse

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 18, 2025

Doublecortin is a neuronal microtubule-associated protein that regulates microtubule structure in neurons. Mutations cause lissencephaly and subcortical band heterotopia by impairing migration. We use CRISPR/Cas9 to knock-out the gene induced pluripotent stem cells differentiate into cortical DCX-KO neurons show reduced velocities of nuclear movements an increased number neurites early development, consistent with previous findings. Neurite branching regulated host proteins, as well polymerization dynamics. However, EB comet dynamics are unchanged Rather, we observe significant reduction α-tubulin polyglutamylation Polyglutamylation levels rescued expression or TTLL11, glutamylase. Using U2OS orthogonal model system, DCX TTLL11 act synergistically promote polyglutamylation. propose acts positive regulator restricts neurite branching. Our results indicate unexpected role for homeostasis tubulin code. Lissencephaly severe neurodevelopmental disease often caused mutations Dcx gene. human cellular lissencephaly, authors report activating

Language: Английский

Citations

1
Identification of a basal body‐localized epsilon‐tubulin in Leishmania DOI Open Access
Arunava Seth, Anubhab Das, Rupak Datta

et al.

FEBS Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Epsilon‐tubulins have been identified in only a few organisms to date. We an epsilon‐tubulin Leishmania major (Lmε‐tubulin) exhibiting significant sequence similarity and conservation of functional domains with its counterparts. It is expressed both promastigote amastigote forms the parasite. For localization studies, we generated strain expressing endogenous Lmε‐tubulin tagged mNeonGreen by CRISPR‐Cas9. Imaging this revealed that located near kinetoplast at flagellar base, indicating basal body localization. not part microtubular network since remained unaltered upon treatment nocodazole, microtubule disruptor. This first experimental identification ε‐tubulin but entire Trypanosomatidae family.

Language: Английский

Citations

1
NEKL-4 regulates microtubule stability and mitochondrial health in ciliated neurons DOI Creative Commons
Kaiden M. Power, Ken C. Q. Nguyen,

Andriele Silva

et al.

The Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 223(9)

Published: May 20, 2024

Ciliopathies are often caused by defects in the ciliary microtubule core. Glutamylation is abundant cilia, and its dysregulation may contribute to ciliopathies neurodegeneration. Mutation of deglutamylase CCP1 causes infantile-onset In C. elegans, ccpp-1 loss age-related degradation that suppressed a mutation conserved NEK10 homolog nekl-4. NEKL-4 absent from yet it negatively regulates stability via an unknown, glutamylation-independent mechanism. We show was mitochondria-associated. Additionally, nekl-4 mutants had longer mitochondria, higher baseline mitochondrial oxidation state, ccpp-1∆ mutant lifespan extension response oxidative stress. A kinase-dead nekl-4(KD) ectopically localized cilia rescued degenerating doublet B-tubules. nondegradable nekl-4(PEST∆) resembled with dye-filling B-tubule breaks. The Dyf phenotype depolymerizing kinesin-8 KLP-13/KIF19A. conclude influences activating kinesins promoting homeostasis.

Language: Английский

Citations

6
Design, synthesis and cytotoxic activity of molecular hybrids based on quinolin-8-yloxy and cinnamide hybrids and their apoptosis inducing property DOI Creative Commons
Dalal Nasser Binjawhar, Fawziah A. Al‐Salmi,

Ola A. Abu Ali

et al.

RSC Advances, Journal Year: 2024, Volume and Issue: 14(16), P. 11443 - 11451

Published: Jan. 1, 2024

A sequence of novel quinoline-8-yloxy and cinnamide hybrids has been synthesized evaluated for in vitro cytotoxicity against HepG2 liver cancer cells.

Language: Английский

Citations

5
A cytoskeleton symphony: Actin and microtubules in microglia dynamics and aging DOI Creative Commons
Renato Socodato, João B. Relvas

Progress in Neurobiology, Journal Year: 2024, Volume and Issue: 234, P. 102586 - 102586

Published: Feb. 16, 2024

Microglia dynamically reorganize their cytoskeleton to perform essential functions such as phagocytosis of toxic protein aggregates, surveillance the brain parenchyma, and regulation synaptic plasticity during neuronal activity bursts. Recent studies have shed light on critical role microtubule in microglial reactivity function, revealing key regulators like cyclin-dependent kinase 1 centrosomal nucleation remodeling microtubules activated microglia. Concurrently, actin is also pivotal, particularly context small GTPases RhoA, Rac1, Cdc42 actin-binding molecules profilin-1 cofilin. This article delves into intricate molecular landscape microtubules, exploring synergistic roles driving cytoskeletal dynamics. We propose a more integrated view cooperation, which fundamental understanding functional coherence its pivotal propelling homeostasis. Furthermore, we discuss how alterations dynamics aging disease states could far-reaching implications for function. By unraveling complexities microglia dynamics, can deepen our health disease, offering insights potential therapeutic interventions neurologic disorders.

Language: Английский

Citations

5
Excessive tubulin glutamylation leads to progressive cone-rod dystrophy and loss of outer segment integrity DOI

Rawaa Aljammal,

Thamaraiselvi Saravanan,

Tongju Guan

et al.

Human Molecular Genetics, Journal Year: 2024, Volume and Issue: 33(9), P. 802 - 817

Published: Jan. 31, 2024

Abstract Mutations in Cytosolic Carboxypeptidase-like Protein 5 (CCP5) are associated with vision loss humans. To decipher the mechanisms behind CCP5-associated blindness, we generated a novel mouse model lacking CCP5. In this model, found that increased tubulin glutamylation led to progressive cone-rod dystrophy, cones showing more pronounced and earlier functional than rod photoreceptors. The observed reduction was not due cell death, levels, or mislocalization of major phototransduction proteins. Instead, caused shortened photoreceptor axonemes formation numerous abnormal membranous whorls disrupted integrity outer segments (OS). Ultimately, excessive photoreceptors, affecting severely rods. Our results highlight importance maintaining for normal function. Furthermore, demonstrate murine cone photoreceptors sensitive levels rods, suggesting an essential role axoneme structural segment. This study provides valuable insights into diseases linked glutamylation.

Language: Английский

Citations

4
Map-1a regulates Sertoli cell BTB dynamics through the cytoskeletal organization of microtubule and F-actin DOI Creative Commons
Lingling Wang, Ming Yan,

Tiao Bu

et al.

Reproductive Biology and Endocrinology, Journal Year: 2024, Volume and Issue: 22(1)

Published: April 3, 2024

Abstract Microtubule-associated protein 1a (Map1a) is a microtubule (MT) regulatory that binds to the MT protofilaments in mammalian cells promote stabilization. Maps work with cleavage proteins and other catastrophe-inducing confer dynamics support changes Sertoli cell shape sustain spermatogenesis. However, no functional studies are found literature probe its role Using an RNAi approach, coupled use of toxicant-induced testis (in vivo)- vitro)-injury models, RNA-Seq analysis, transcriptome profiling, relevant bioinformatics immunofluorescence pertinent biochemical assays for cytoskeletal organization, we have delineated Map1a testes. was shown structural knockdown (KD) also perturbed organization actin, vimentin, septin cytoskeletons as these intimately related, working concert More importantly, cadmium-induced injury across cytoplasm associated disruptive distribution surge p-p38-MAPK (phosphorylated p38-mitogen-activated kinase) expression but not total p38-MAPK. These findings thus notion activation involved injury. This conclusion supported by using doramapimod, specific p38-MAPK phosphorylation (activation) inhibitor, which capable restoring MTs cytoplasm. In summary: this study provides mechanistic insights regarding restoration male infertility.

Language: Английский

Citations

4
Microtubules: Decoding tubulin diversity with help from an amoeba DOI
Gil Henkin, Simone Reber

Current Biology, Journal Year: 2025, Volume and Issue: 35(2), P. R69 - R71

Published: Jan. 1, 2025

Language: Английский

Citations

0
Emerging roles for tubulin PTMs in neuronal function and neurodegenerative disease DOI
Jia Jie Teoh, Francesca Bartolini

Current Opinion in Neurobiology, Journal Year: 2025, Volume and Issue: 90, P. 102971 - 102971

Published: Jan. 24, 2025

Language: Английский

Citations

0