Chemosensory sensilla of the Drosophila wing express a candidate ionotropic pheromone receptor DOI Creative Commons
Zhe He, Yichen Luo,

Xueying Shang

et al.

PLoS Biology, Journal Year: 2019, Volume and Issue: 17(5), P. e2006619 - e2006619

Published: May 21, 2019

The Drosophila wing was proposed to be a taste organ more than 35 years ago, but there has been remarkably little study of its role in chemoreception. We carry out differential RNA-seq analysis row sensilla on the anterior margin and find expression many genes associated with pheromone chemical perception. To ask whether these might receive pheromonal input, we devised dye-transfer paradigm found that large, hydrophobic molecules comparable pheromones can transferred from one fly another. One gene, Ionotropic receptor (IR)52a, is coexpressed neurons fruitless, marker sexual circuitry; IR52a also expressed legs. Mutation optogenetic silencing IR52a+ decrease levels male behavior. Optogenetic activation induces males show courtship toward other and, remarkably, females another species. Surprisingly, required for normal mated copulation, which normally occurs at very low levels. Unlike chemoreceptors act inhibit male–male interactions promote male–female interactions, acts both females, as well interactions. Moreover, override circuitry suppresses behavior unproductive targets. Circuit mapping Ca2+ imaging using trans-Tango system reveals second-order projections subesophageal zone (SEZ), some are sexually dimorphic. activates SEZ. Taken together, this provides molecular description chemosensory greatly understudied defines gene regulates fly.

Language: Английский

Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons DOI Creative Commons
Darya Task, Chun‐Chieh Lin, Alina Vulpe

et al.

eLife, Journal Year: 2022, Volume and Issue: 11

Published: April 20, 2022

olfactory neurons have long been thought to express only one chemosensory receptor gene family. There are two main families in

Language: Английский

Citations

101
Structural basis for sugar perception by Drosophila gustatory receptors DOI
Demin Ma, Meiqin Hu, Xiaotong Yang

et al.

Science, Journal Year: 2024, Volume and Issue: 383(6685)

Published: Feb. 2, 2024

Insects rely on a family of seven transmembrane proteins called gustatory receptors (GRs) to encode different taste modalities, such as sweet and bitter. We report structures

Language: Английский

Citations

22
A Drosophila computational brain model reveals sensorimotor processing DOI Creative Commons
Philip K. Shiu, Gabriella R Sterne, Nico Spiller

et al.

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 210 - 219

Published: Oct. 2, 2024

Language: Английский

Citations

19
An expression atlas of variant ionotropic glutamate receptors identifies a molecular basis of carbonation sensing DOI Creative Commons
Juan Antonio Sánchez‐Alcañiz, Ana F. Silbering, Vincent Croset

et al.

Nature Communications, Journal Year: 2018, Volume and Issue: 9(1)

Published: Oct. 8, 2018

Abstract Through analysis of the Drosophila ionotropic receptors (IRs), a family variant glutamate receptors, we reveal that most IRs are expressed in peripheral neuron populations diverse gustatory organs larvae and adults. We characterise IR56d, which defines two anatomically-distinct classes proboscis: one responds to carbonated solutions fatty acids while other represents subset sugar- acid-sensing cells. Mutational indicates together with broadly-expressed co-receptors IR25a IR76b, is essential for physiological responses carbonation acids, but not sugars. further demonstrate both promote IR56d-dependent attraction flies, through different behavioural outputs. Our work provides toolkit investigating taste functions IRs, these required sensing, illustrates how system uses combinatorial expression sensory molecules distinct neurons coordinate behaviour.

Language: Английский

Citations

153
A complex peripheral code for salt taste in Drosophila DOI Creative Commons

Alexandria Jaeger,

Molly Stanley, Zachary F. Weiss

et al.

eLife, Journal Year: 2018, Volume and Issue: 7

Published: Oct. 11, 2018

Each taste modality is generally encoded by a single, molecularly defined, population of sensory cells. However, salt stimulates multiple pathways in mammals and insects, suggesting more complex code for taste. Here, we examine coding Drosophila. After creating comprehensive molecular map comprised five discrete neuron classes across the fly labellum, find that four are activated salt: two exhibiting characteristics ‘low salt’ cells, ‘high classes. Behaviorally, low attraction depends primarily on ‘sweet’ neurons, with additional input from neurons expressing ionotropic receptor IR94e. High avoidance mediated ‘bitter’ glutamatergic Ppk23. Interestingly, impact these prior consumption. These results support model flies combinatorially integrates inputs cell types to afford robust flexible behaviors.

Language: Английский

Citations

149
High Dietary Sugar Reshapes Sweet Taste to Promote Feeding Behavior in Drosophila melanogaster DOI Creative Commons
Christina E. May, Anoumid Vaziri,

Yong Lin

et al.

Cell Reports, Journal Year: 2019, Volume and Issue: 27(6), P. 1675 - 1685.e7

Published: May 1, 2019

Recent studies find that sugar tastes less intense to humans with obesity, but whether this sensory change is a cause or consequence of obesity unclear. To tackle question, we study the effects high diet on sweet taste sensation and feeding behavior in Drosophila melanogaster. On diet, fruit flies have lower responses stimuli, overconsume food, develop obesity. Excess dietary sugar, not sweetness alone, caused deficits overeating via cell-autonomous action sensor O-linked N-Acetylglucosamine (O-GlcNAc) transferase (OGT) sweet-sensing neurons. Correcting by manipulating excitability gustatory neurons levels OGT protected animals from diet-induced Our work demonstrates reshaping excess drives highlights role glucose metabolism neural activity behavior.

Language: Английский

Citations

137
A determining factor for insect feeding preference in the silkworm, Bombyx mori DOI Creative Commons
Zhongjie Zhang, Shuaishuai Zhang,

Baolong Niu

et al.

PLoS Biology, Journal Year: 2019, Volume and Issue: 17(2), P. e3000162 - e3000162

Published: Feb. 27, 2019

Feeding preference is critical for insect adaptation and survival. However, little known regarding the determination of feeding preference, genetic basis poorly understood. As a model lepidopteran with economic importance, domesticated silkworm, Bombyx mori, well-known monophagous that predominantly feeds on fresh mulberry leaves. This species-specific provides an excellent investigation host-plant selection insects, although molecular mechanism underlying this phenomenon remains unknown. Here, we describe gene GR66, which encodes putative bitter gustatory receptor (GR) responsible mulberry-specific B. mori. With aid transposon-based, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) system, GR66 locus was genetically mutated, homozygous mutant silkworm strains truncated 66 (GR66) proteins were established. larvae acquired new activity, exhibiting ability to feed number plant species in addition leaves, including fruits grain seeds are not normally consumed by wild-type (WT) silkworms. Furthermore, choice assay revealed lost their specificity mulberry. Overall, our findings provide first phenotypic evidences single GR major factor affecting preference.

Language: Английский

Citations

111
Neural Control and Modulation of Thirst, Sodium Appetite, and Hunger DOI Creative Commons
Vineet Augustine, Sangjun Lee, Yuki Oka

et al.

Cell, Journal Year: 2020, Volume and Issue: 180(1), P. 25 - 32

Published: Jan. 1, 2020

Language: Английский

Citations

106
The ion channel ppk301 controls freshwater egg-laying in the mosquito Aedes aegypti DOI Creative Commons
Benjamin J. Matthews, Meg A. Younger, Leslie B. Vosshall

et al.

eLife, Journal Year: 2019, Volume and Issue: 8

Published: May 21, 2019

Female Aedes aegypti mosquitoes are deadly vectors of arboviral pathogens and breed in containers freshwater associated with human habitation. Because high salinity is lethal to offspring, correctly evaluating water purity a crucial parenting decision. We found that the DEG/ENaC channel ppk301 sensory neurons expressing control egg-laying initiation choice Ae. aegypti. Using calcium imaging, we ppk301-expressing cells show ppk301-dependent responses but, unexpectedly, also respond salt ppk301-independent fashion. This suggests instructive for at low-salt concentrations, but pathway responsible inhibiting high-salt concentrations. Water key resource insect survival understanding how interact different behaviors an opportunity study evolution chemosensory systems.

Language: Английский

Citations

98
Sensory Discrimination of Blood and Floral Nectar by Aedes aegypti Mosquitoes DOI Creative Commons
Veronica Jové, Zhongyan Gong, Felix J.H. Hol

et al.

Neuron, Journal Year: 2020, Volume and Issue: 108(6), P. 1163 - 1180.e12

Published: Oct. 12, 2020

Language: Английский

Citations

79