Chemosensory sensilla of the Drosophila wing express a candidate ionotropic pheromone receptor DOI Creative Commons
Zhe He, Yichen Luo,

Xueying Shang

et al.

PLoS Biology, Journal Year: 2019, Volume and Issue: 17(5), P. e2006619 - e2006619

Published: May 21, 2019

The Drosophila wing was proposed to be a taste organ more than 35 years ago, but there has been remarkably little study of its role in chemoreception. We carry out differential RNA-seq analysis row sensilla on the anterior margin and find expression many genes associated with pheromone chemical perception. To ask whether these might receive pheromonal input, we devised dye-transfer paradigm found that large, hydrophobic molecules comparable pheromones can transferred from one fly another. One gene, Ionotropic receptor (IR)52a, is coexpressed neurons fruitless, marker sexual circuitry; IR52a also expressed legs. Mutation optogenetic silencing IR52a+ decrease levels male behavior. Optogenetic activation induces males show courtship toward other and, remarkably, females another species. Surprisingly, required for normal mated copulation, which normally occurs at very low levels. Unlike chemoreceptors act inhibit male–male interactions promote male–female interactions, acts both females, as well interactions. Moreover, override circuitry suppresses behavior unproductive targets. Circuit mapping Ca2+ imaging using trans-Tango system reveals second-order projections subesophageal zone (SEZ), some are sexually dimorphic. activates SEZ. Taken together, this provides molecular description chemosensory greatly understudied defines gene regulates fly.

Language: Английский

A molecular mechanism for high salt taste in Drosophila DOI Creative Commons
Sasha A.T. McDowell, Molly Stanley, Michael D. Gordon

et al.

Current Biology, Journal Year: 2022, Volume and Issue: 32(14), P. 3070 - 3081.e5

Published: June 29, 2022

Language: Английский

Citations

42
Evolution of chemosensory tissues and cells across ecologically diverse Drosophilids DOI Creative Commons
Gwénaëlle Bontonou, Bastien Saint‐Leandre, Tane Kafle

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 5, 2024

Abstract Chemosensory tissues exhibit significant between-species variability, yet the evolution of gene expression and cell types underlying this diversity remain poorly understood. To address these questions, we conducted transcriptomic analyses five chemosensory from six Drosophila species integrated findings with single-cell datasets. While stabilizing selection predominantly shapes transcriptomes, thousands genes in each tissue have evolved differences. Genes that changed one often multiple other but at different past epochs are more likely to be type-specific than unchanged genes. Notably, chemosensory-related undergone widespread changes, numerous species-specific gains/losses including novel chemoreceptors patterns. Sex differences also pervasive, a D. melanogaster- specific excess male-biased sensory muscle cells its forelegs. Together, our provide new insights for understanding evolutionary changes both global individual levels.

Language: Английский

Citations

11
The neuroscience of sugars in taste, gut-reward, feeding circuits, and obesity DOI
Ranier Gutiérrez, Esmeralda Fonseca, Sidney A. Simon

et al.

Cellular and Molecular Life Sciences, Journal Year: 2020, Volume and Issue: 77(18), P. 3469 - 3502

Published: Jan. 31, 2020

Language: Английский

Citations

61
Evolutionary shifts in taste coding in the fruit pest Drosophila suzukii DOI Creative Commons
Hany K. M. Dweck, Gaëlle J.S. Talross, Wanyue Wang

et al.

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Feb. 22, 2021

Although most Drosophila species lay eggs in overripe fruit, the agricultural pest suzukii lays ripe fruit. We found that changes bitter taste perception have accompanied this adaptation. show bitter-sensing mutants of melanogaster undergo a shift egg laying preference toward D. has lost 20% sensilla from labellum, major organ head. Physiological responses to various compounds are lost. Responses strawberry purées two classes sensilla. Egg is not deterred by deter other species. Profiling labellar transcriptomes reveals reduced expression several Gr genes ( gustatory receptors ). These findings support model which early ripening stages species, but loss response contributes adaptation

Language: Английский

Citations

49
Dopamine modulation of sensory processing and adaptive behavior in flies DOI Creative Commons

K.P. Siju,

Jean‐François De Backer, Ilona C Grunwald Kadow

et al.

Cell and Tissue Research, Journal Year: 2021, Volume and Issue: 383(1), P. 207 - 225

Published: Jan. 1, 2021

Abstract Behavioral flexibility for appropriate action selection is an advantage when animals are faced with decisions that will determine their survival or death. In order to arrive at the right decision, evaluate information from external environment, internal state, and past experiences. How these different signals integrated modulated in brain, how context- state-dependent behavioral controlled poorly understood questions. Studying molecules help convey integrate such neural circuits important way approach Many years of work model organisms have shown dopamine a critical neuromodulator (reward based) associative learning. However, recent findings vertebrates invertebrates demonstrated complexity heterogeneity dopaminergic neuron populations functional implications many adaptive behaviors survival. For example, neurons can sensory information, states, learned experience decision making circuitry. Several advances methodologies availability synaptic level connectome whole-brain circuitry Drosophila melanogaster make fly attractive system study roles behavior. particular, learning memory center—the mushroom body—is richly innervated by enable it multi-modal according state context, modulate decision-making

Language: Английский

Citations

45
Ir56d-dependent fatty acid responses in Drosophila uncover taste discrimination between different classes of fatty acids DOI Creative Commons
Elizabeth Brown, Kreesha D. Shah, Justin Palermo

et al.

eLife, Journal Year: 2021, Volume and Issue: 10

Published: May 5, 2021

Chemosensory systems are critical for evaluating the caloric value and potential toxicity of food. While animals can discriminate between thousands odors, much less is known about discriminative capabilities taste systems. Fats sugars represent calorically potent attractive food sources that contribute to hedonic feeding. Despite differences in nutritional fats sugars, ability system different rewarding tastants thought be limited. In Drosophila, neurons expressing ionotropic receptor 56d (IR56d) required reflexive behavioral responses medium-chain fatty acid, hexanoic acid. Here, we tested whether flies classes acids using an aversive memory assay. Our results indicate able from both short- long-chain acids, but not other acids. IR56d broadly responsive short-, medium-, genetic deletion selectively disrupts response Further, IR56d+ GR64f+ necessary proboscis extension (PER) GR64f dispensable PER indicating involvement one or more neurons. Together, these findings reveal acid taste, discrimination occurs through differential activation shared populations study uncovers a capacity encode tastant identity within category.

Language: Английский

Citations

43
Identification of a gustatory receptor tuned to sinigrin in the cabbage butterfly Pieris rapae DOI Creative Commons
Jun Yang, Hao Guo, Nan‐Ji Jiang

et al.

PLoS Genetics, Journal Year: 2021, Volume and Issue: 17(7), P. e1009527 - e1009527

Published: July 15, 2021

Glucosinolates are token stimuli in host selection of many crucifer specialist insects, but the underlying molecular basis for these insects remains enigmatic. Using a combination behavioral, electrophysiological, and methods, we investigate glucosinolate receptors cabbage butterfly Pieris rapae . Sinigrin, as potent feeding stimulant, elicited activity larval maxillary lateral sensilla styloconica, well adult medial tarsal sensilla. Two P gustatory receptor genes PrapGr28 PrapGr15 were identified with high expression female tarsi, subsequent functional analyses showed that Xenopus oocytes only expressing had specific responses to sinigrin; when ectopically expressed Drosophila sugar sensing neurons, conferred sinigrin sensitivity neurons. RNA interference experiments further knockdown reduced sinigrin. Taken together, conclude is tuned , which paves way revealing relationships between plants their insects.

Language: Английский

Citations

42
A neuronal ensemble encoding adaptive choice during sensory conflict in Drosophila DOI Creative Commons
Preeti Sareen, Li Yan McCurdy, Michael N. Nitabach

et al.

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: July 5, 2021

Abstract Feeding decisions are fundamental to survival, and decision making is often disrupted in disease. Here, we show that neural activity a small population of neurons projecting the fan-shaped body higher-order central brain region Drosophila represents food choice during sensory conflict. We found deprived flies made tradeoffs between appetitive aversive values food. identified an upstream neuropeptidergic dopaminergic network relays internal state other decision-relevant information specific subset neurons. These were strongly inhibited by taste rejected choice, suggesting they encode behavioral choice. Our findings reveal responses choices determined not only quality, but also previous experience (including outcome) hunger state, which integrated before relay downstream motor circuits for implementation.

Language: Английский

Citations

42
Taste quality and hunger interactions in a feeding sensorimotor circuit DOI Creative Commons
Philip K. Shiu, Gabriella R Sterne, Stefanie Engert

et al.

eLife, Journal Year: 2022, Volume and Issue: 11

Published: July 6, 2022

Taste detection and hunger state dynamically regulate the decision to initiate feeding. To study how context-appropriate feeding decisions are generated, we combined synaptic resolution circuit reconstruction with targeted genetic access specific neurons elucidate a gustatory sensorimotor for initiation in adult Drosophila melanogaster. This connects sensory proboscis motor through three intermediate layers. Most this pathway necessary sufficient extension, behavior, respond selectively sugar taste detection. Pathway activity is amplified by signals that act at select second-order promote food-deprived animals. In contrast, inhibited bitter impinges on premotor neurons, illuminating local motif weighs adjust behavioral outcomes. Together, these studies reveal central mechanisms integration of external internal nutritive flexibly execute critical decision.

Language: Английский

Citations

36
The neuronal logic of how internal states control food choice DOI
Daniel Münch, Dennis Goldschmidt, Carlos Ribeiro

et al.

Nature, Journal Year: 2022, Volume and Issue: 607(7920), P. 747 - 755

Published: July 6, 2022

Language: Английский

Citations

33