Chemosensory sensilla of the Drosophila wing express a candidate ionotropic pheromone receptor DOI Creative Commons
Zhe He, Yichen Luo,

Xueying Shang

et al.

PLoS Biology, Journal Year: 2019, Volume and Issue: 17(5), P. e2006619 - e2006619

Published: May 21, 2019

The Drosophila wing was proposed to be a taste organ more than 35 years ago, but there has been remarkably little study of its role in chemoreception. We carry out differential RNA-seq analysis row sensilla on the anterior margin and find expression many genes associated with pheromone chemical perception. To ask whether these might receive pheromonal input, we devised dye-transfer paradigm found that large, hydrophobic molecules comparable pheromones can transferred from one fly another. One gene, Ionotropic receptor (IR)52a, is coexpressed neurons fruitless, marker sexual circuitry; IR52a also expressed legs. Mutation optogenetic silencing IR52a+ decrease levels male behavior. Optogenetic activation induces males show courtship toward other and, remarkably, females another species. Surprisingly, required for normal mated copulation, which normally occurs at very low levels. Unlike chemoreceptors act inhibit male–male interactions promote male–female interactions, acts both females, as well interactions. Moreover, override circuitry suppresses behavior unproductive targets. Circuit mapping Ca2+ imaging using trans-Tango system reveals second-order projections subesophageal zone (SEZ), some are sexually dimorphic. activates SEZ. Taken together, this provides molecular description chemosensory greatly understudied defines gene regulates fly.

Language: Английский

Taste coding of heavy metal ion-induced avoidance in Drosophila DOI Creative Commons
Xiaonan Li, Yuanjie Sun, Shan Gao

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(5), P. 106607 - 106607

Published: April 7, 2023

Increasing pollution of heavy metals poses great risks to animals globally. Their survival likely relies on an ability detect and avoid harmful metal ions (HMIs). Currently, little is known about the neural mechanisms HMI detection. Here, we show that Drosophila related species Drosophilidae actively toxic HMIs at micromolar concentrations. The high sensitivity biologically relevant. Particularly, their cadmium as most bitter substance, denatonium. Detection in food requires Gr66a+ gustatory neurons but independent bitter-taste receptors. In these neurons, ionotropic receptors IR76b, IR25a, IR7a are required for perception metals. Furthermore, IR47a mediates activation a distinct group non-Gr66a+ elicited by HMIs. Together, our findings reveal surprising taste quality represented noxious ions.

Language: Английский

Citations

20
Descending GABAergic pathway links brain sugar-sensing to peripheral nociceptive gating in Drosophila DOI Creative Commons
Mami Nakamizo-Dojo, Kenichi Ishii, Jiro Yoshino

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Oct. 16, 2023

Although painful stimuli elicit defensive responses including escape behavior for survival, starved animals often prioritize feeding over even in a noxious environment. This behavioral priority is typically mediated by suppression of inputs through descending control the brain, yet underlying molecular and cellular mechanisms are incompletely understood. Here we identify cluster GABAergic neurons Drosophila larval designated as SEZ-localized Descending (SDGs), that project axons onto axon terminals peripheral nociceptive prevent presynaptic activity GABAB receptors. Remarkably, glucose to larvae causes sustained activation SDGs glucose-sensing subsequent insulin signaling SDGs, which attenuates nociception thereby suppresses response multiple stimuli. These findings illustrate neural mechanism sugar sensing brain engages gating achieve hierarchical interaction between behavior.

Language: Английский

Citations

20
Recent advances in investigating odor-taste interactions: Psychophysics, neuroscience, and microfluidic techniques DOI
Yan Ping Chen, Ziyu Ding,

Yashu Yu

et al.

Trends in Food Science & Technology, Journal Year: 2023, Volume and Issue: 138, P. 500 - 510

Published: June 24, 2023

Language: Английский

Citations

18
Selective integration of diverse taste inputs within a single taste modality DOI Creative Commons

Julia U. Deere,

Arvin A Sarkissian,

Meifeng Yang

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Jan. 24, 2023

A fundamental question in sensory processing is how different channels of input are processed to regulate behavior. Different may converge onto common downstream pathways drive the same behaviors, or they activate separate distinct behaviors. We investigated this Drosophila bitter taste system, which contains diverse bitter-sensing cells residing organs. First, we optogenetically activated subsets neurons within each organ. These elicited broad and highly overlapping behavioral effects, suggesting that pathways, but also observed differences argue for biased convergence. Consistent with these results, transsynaptic tracing revealed organs connect connectivity. one type neuron projects higher brain. integrate from multiple specific taste-related then traced circuits, providing first glimpse into Together, results reveal inputs selectively integrated early circuit, enabling pooling information, while circuit diverges have roles.

Language: Английский

Citations

17
Chemosensory sensilla of the Drosophila wing express a candidate ionotropic pheromone receptor DOI Creative Commons
Zhe He, Yichen Luo,

Xueying Shang

et al.

PLoS Biology, Journal Year: 2019, Volume and Issue: 17(5), P. e2006619 - e2006619

Published: May 21, 2019

The Drosophila wing was proposed to be a taste organ more than 35 years ago, but there has been remarkably little study of its role in chemoreception. We carry out differential RNA-seq analysis row sensilla on the anterior margin and find expression many genes associated with pheromone chemical perception. To ask whether these might receive pheromonal input, we devised dye-transfer paradigm found that large, hydrophobic molecules comparable pheromones can transferred from one fly another. One gene, Ionotropic receptor (IR)52a, is coexpressed neurons fruitless, marker sexual circuitry; IR52a also expressed legs. Mutation optogenetic silencing IR52a+ decrease levels male behavior. Optogenetic activation induces males show courtship toward other and, remarkably, females another species. Surprisingly, required for normal mated copulation, which normally occurs at very low levels. Unlike chemoreceptors act inhibit male–male interactions promote male–female interactions, acts both females, as well interactions. Moreover, override circuitry suppresses behavior unproductive targets. Circuit mapping Ca2+ imaging using trans-Tango system reveals second-order projections subesophageal zone (SEZ), some are sexually dimorphic. activates SEZ. Taken together, this provides molecular description chemosensory greatly understudied defines gene regulates fly.

Language: Английский

Citations

54