Obesity and Aging in the Drosophila Model

International Journal of Molecular Sciences, Journal Year: 2018, Volume and Issue: 19(7), P. 1896 - 1896

Published: June 27, 2018

Being overweight increases the risk of many metabolic disorders, but how it affects lifespan is not completely clear. Not all obese people become ill, and exact mechanism that turns excessive fat storage into a health-threatening state remains unknown. Drosophila melanogaster has served as an excellent model for diseases, including obesity, diabetes, hyperglycemia-associated such cardiomyopathy or nephropathy. Here, we review connections between aging in different types fly obesity. Whereas obesity induced by high-fat high-sugar diet associated with hyperglycemia, cardiomyopathy, some cases, shortening lifespan, there are also examples which correlates longevity. Transgenic lines downregulations insulin/insulin-like growth factor (IIS) target rapamycin (TOR) signaling pathways, flies reared under dietary restriction, even certain longevity selection obese, yet long-lived. The mechanisms underlie differential lifespans distinct remain to be elucidated, turnover, inflammatory dysregulations glucose metabolism may play key roles. Altogether, study physiology adiposity both health disease.

Language: Английский

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Proteomics of protein trafficking by in vivo tissue-specific labeling

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: April 22, 2021

Language: Английский

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A perinuclear microtubule-organizing centre controls nuclear positioning and basement membrane secretion

Nature Cell Biology, Journal Year: 2020, Volume and Issue: 22(3), P. 297 - 309

Published: Feb. 17, 2020

Language: Английский

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Proteasome stress in skeletal muscle mounts a long-range protective response that delays retinal and brain aging

Cell Metabolism, Journal Year: 2021, Volume and Issue: 33(6), P. 1137 - 1154.e9

Published: March 26, 2021

Language: Английский

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Dynamic tracking and identification of tissue-specific secretory proteins in the circulation of live mice

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: Sept. 1, 2021

Secretory proteins are an essential component of interorgan communication networks that regulate animal physiology. Current approaches for identifying secretory from specific cell and tissue types largely limited to in vitro or ex vivo models which often fail recapitulate biology. As such, there is mounting interest developing analytical tools can provide accurate information on the origin, identity, spatiotemporal dynamics proteins. Here, we describe iSLET (in situ protein Labeling via ER-anchored TurboID) selectively labels transit through classical pathway catalytic actions Sec61b-TurboID, a proximity labeling enzyme anchored ER lumen. To validate whole-body system, express mouse liver demonstrate efficient could be tracked identified within circulating blood plasma. Furthermore, proteomic analysis labeled secretome enriched plasma highly consistent with previous reports profiles. Taken together, versatile powerful tool studying proteins, valuable class biomarkers therapeutic targets.

Language: Английский

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Discovering signaling mechanisms governing metabolism and metabolic diseases with Drosophila

Cell Metabolism, Journal Year: 2021, Volume and Issue: 33(7), P. 1279 - 1292

Published: June 16, 2021

Language: Английский

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Understanding the molecular basis of anorexia and tissue wasting in cancer cachexia

Experimental & Molecular Medicine, Journal Year: 2022, Volume and Issue: 54(4), P. 426 - 432

Published: April 6, 2022

Abstract Cancer cachexia syndrome is a major cause of morbidity and mortality in cancer patients the advanced stage. It devastating disorder characterized by nutritional impairment, weakness, wasting, it affects treatment success quality life. Two symptoms are anorexia weight loss. Weight loss not reversed through increased food intake, suggesting that regulated independent molecular mechanisms. Although wasting phenotype mostly occurs skeletal muscle adipose tissue, other organs, such as brain, liver, pancreas, heart, gut, also involved cachexia. Thus, multiorgan syndrome. basis cachexia-induced known, mechanism underlying poorly understood. Here, we highlight our recent discovery new which tumor-derived humoral factor induces regulating feeding-related neuropeptide hormones brain. Furthermore, elucidated process precedes tissue This review article aims to provide an overview key mechanisms caused

Language: Английский

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Tumour-derived Dilp8/INSL3 induces cancer anorexia by regulating feeding neuropeptides via Lgr3/8 in the brain

Nature Cell Biology, Journal Year: 2021, Volume and Issue: 23(2), P. 172 - 183

Published: Feb. 1, 2021

Language: Английский

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Endocrine cybernetics: neuropeptides as molecular switches in behavioural decisions

Open Biology, Journal Year: 2022, Volume and Issue: 12(7)

Published: July 1, 2022

Plasticity in animal behaviour relies on the ability to integrate external and internal cues from changing environment hence modulate activity synaptic circuits of brain. This context-dependent neuromodulation is largely based non-synaptic signalling with neuropeptides. Here, we describe select peptidergic systems Drosophila brain that act at different levels a hierarchy associated physiology. These regions, such as central complex mushroom bodies, which supervise specific behaviours. At top level there are small numbers large neurons arborize widely multiple areas orchestrate or global state manner. bottom local provide executive sensory gain intrinsically restricted parts neuronal circuits. The orchestrating receive interoceptive signals mediate energy sleep homeostasis, metabolic circadian timing, well affect food search, aggression mating. Some these can be triggers conflicting behaviours mating versus aggression, feeding, participate circuits, enabling choices switches.

Language: Английский

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split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(24)

Published: June 5, 2023

The split-Gal4 system allows for intersectional genetic labeling of highly specific cell types and tissues in Drosophila. However, the existing system, unlike standard Gal4 cannot be repressed by Gal80, therefore controlled temporally. This lack temporal control precludes experiments which a manipulation must restricted to timepoints. Here, we describe based on self-excising split-intein, drives transgene expression as strongly current reagents, yet is repressible Gal80. We demonstrate potent inducibility "split-intein Gal4" vivo using both fluorescent reporters via reversible tumor induction gut. Further, show that our split-intein can extended drug-inducible GeneSwitch providing an independent method with inducible control. also used generate type-specific drivers silico predictions generated single-cell RNAseq (scRNAseq) datasets, algorithm ("Two Against Background" or TAB) predict cluster-specific gene pairs across multiple tissue-specific scRNA datasets. provide plasmid toolkit efficiently create either CRISPR knock-ins target genes enhancer fragments. Altogether, creation are inducible/repressible.

Language: Английский

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