Biochemical and Biophysical Research Communications, Journal Year: 2025, Volume and Issue: 755, P. 151573 - 151573
Published: March 1, 2025
Language: Английский
Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown
Published: Nov. 29, 2023
Abstract Candida auris is an emergent human fungal pathogen of growing concern due to common drug resistance all major antifungal classes. Although amphotericin B (AMB) has been detected in 30 60% clinical isolates C. , mechanisms AMB remain poorly characterized. Here we present a large-scale investigation how can be acquired through genetic adaptation. We typed 441 vitro and vivo evolved lineages from four AMB-susceptible strains different clades. show great diversity responses with magnitude- strain-dependent fitness trade-offs. Genotyping membrane sterol analyses selected types alterations. Using novel, plasmid-based CRISPR-Cas9 allele editing method Cas9-RNP meditated gene deletions, that variation several biosynthesis regulators including ERG6, NCP1, ERG11, ERG3, HMG1, ERG10 ERG12 . Additionally, aneuploidies chromosomes 4 6 emerge during evolution. By leveraging trade-off phenotyping mathematical modelling the environment treatment, evaluated potential establish resistant infections discover mechanism compensation. Variation CDC25 substantially enhanced capacity infection may have played role facilitating sole documented case treatment In summary, our findings compensation along modulating represent risk for failure clinic.
Language: Английский
Citations
14
Mycoses, Journal Year: 2024, Volume and Issue: 67(6)
Published: June 1, 2024
Candida auris is an emerging multidrug-resistant yeast, frequently causing outbreaks in health care facilities. The pathogen persistently colonises human skin and inanimate surfaces such as catheters, aiding to its spread. Moreover, colonisation a risk factor develop invasive infection.
Language: Английский
Citations
6
Mycopathologia, Journal Year: 2024, Volume and Issue: 189(5)
Published: Aug. 3, 2024
Abstract Fungal infections pose an increasing threat to public health. New pathogens and changing epidemiology are a pronounced risk for nosocomial outbreaks. To investigate clonal transmission between patients trace the source, genotyping is required. In last decades, various typing assays have been developed applied different medically important fungal species. While these methods will be briefly discussed, this review focus on development application of short tandem repeat (STR) genotyping. This method relies amplification comparison highly variable STR markers isolates. For most common pathogens, schemes were compared other methods, like multilocus sequence (MLST), amplified fragment length polymorphism (AFLP) whole genome sequencing (WGS) single nucleotide (SNP) analysis. The pros cons as well requirements solid assay. resolution typing, in general, higher than MLST AFLP, with WGS SNP analysis being gold standard when it comes resolution. Although modern laboratories capable perform little progress has made standardize schemes. Allelic ladders, Aspergillus fumigatus, facilitate results develop global databases. Overall, extremely powerful tool, often complimentary sequencing. Crucial details assay development, its applications merit discussed review.
Language: Английский
Citations
5
Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(10)
Published: Sept. 16, 2024
Language: Английский
Citations
4
Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102586 - 102586
Published: Jan. 1, 2025
Language: Английский
Citations
0
mBio, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 29, 2025
ABSTRACT The emerging fungal pathogen Candida auris is known for its strong skin tropism and resilience against antifungal disinfection treatment, posing a significant challenge healthcare units. Although efforts to identify the effectors of unique pathogenic behavior have been insightful, role high-osmolarity glycerol (HOG) pathway in this context remains unexplored. study by Shivarathri co-workers (R. Shivarathri, M. Chauhan, A. Datta, D. Das et al., mBio 15:e02748-24, 2024, https://doi.org/10.1128/mbio.02748-24 ) sought address gap. This report indeed advances our understanding critical HOG C. pathogenicity emphasizing involvement colonization, biofilm formation, evasion phagocyte attack.
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: Feb. 10, 2025
Language: Английский
Citations
0
Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16
Published: April 9, 2025
The Stephanoascus ciferrii complex, comprising ciferrii, Candida allociferrii, and mucifera, is an emerging fungal pathogen with increasing isolation rates antifungal resistance. However, detailed information about clinical pathogenicity comparisons among the three species are lacking. In order to fill in this gap, study aimed investigate compare of species. Twenty-seven S. complex strains isolated from secretion specimens patients admitted Nanjing Drum Tower Hospital between 2012 2023 were included. According results ITS sequencing, there 15 7 C. 5 mucifera. Antifungal susceptibility testing demonstrated that exhibited high MICs against azole agents, particularly fluconazole, while it showed lower echinocandins. displayed higher caspofungin than allociferrii (P < 0.05). biofilm quantification using crystal violet staining indicated stronger biofilm-forming ability RPMI-1640 medium 0.05), but was no significant difference mucifera or similar metabolic activity by XTT assay. G. mellonella larvae infection experiments revealed survival infected 60%, 50%, 48% at 24 h, 48 72 respectively. Furthermore, lethality caused significantly 0.001). This first describe formation context. Our research reveals prevalence elucidates correlation drug resistance, formation, virulence, thus providing essential empirical evidence for further pathogenic characteristics each treatment strategies.
Language: Английский
Citations
0
Journal of Molecular Biology, Journal Year: 2024, Volume and Issue: 436(8), P. 168513 - 168513
Published: March 5, 2024
Systemic fungal infections are a growing public health threat, and yet viable antifungal drug targets limited as fungi share similar proteome with humans. However, features of RNA metabolism the noncoding transcriptomes in distinctive. For example, harbor highly structured elements that humans lack, such self-splicing introns within key housekeeping genes mitochondria. location function these mitochondrial riboregulatory has largely eluded characterization. Here we used an RNA-structure-based bioinformatics pipeline to identify group I interrupting medically relevant fungi, revealing their fixation handful genetic hotspots ubiquitous presence across divergent phylogenies including all highest priority pathogens Candida albicans, auris, Aspergillus fumigatus Cryptococcus neoformans. We then biochemically characterized two representative from C. albicans demonstrating exceptionally efficient splicing catalysis relative previously-characterized introns. Indeed, intron displays extremely rapid catalytic turnover, even at ambient temperatures physiological magnesium ion concentrations. Our results unmask significant new set players pathogenic suggesting promising type target.
Language: Английский
Citations
3
Clinical Chemistry, Journal Year: 2025, Volume and Issue: 71(1), P. 185 - 191
Published: Jan. 1, 2025
Candida auris is an emerging multidrug-resistant pathogen. Interpretation of susceptibility testing can be difficult since minimum inhibitory concentration (MIC) breakpoints have not been fully established.
Language: Английский
Citations
0