Scientific Reports,
Journal Year:
2022,
Volume and Issue:
12(1)
Published: Oct. 14, 2022
Abstract
The
increasing
demand
for
new
bioactive
compounds
to
combat
the
evolution
of
multi-drug
resistance
(MDR)
requires
research
on
microorganisms
in
different
environments
order
identify
potent
molecules.
In
this
study,
initial
screening
regarding
antimicrobial
activity
44
Actinomycetes
isolates
isolated
from
three
soil
samples
extremely
cold
sites
Morocco
was
carried
out.
Primary
and
secondary
were
performed
against
Candida
albicans
ATCC
60,193,
Escherichia
coli
25,922,
Staphylococcus
aureus
25,923,
Bacillus
cereus
14,579,
other
clinical
MDR
bacteria,
thirteen
phytopathogenic
fungi.
Based
results
obtained,
11
active
selected
further
study.
11microbial
identified
based
morphological
biochemical
characters
their
molecular
identification
using
16S
rRNA
sequence
homology.
UV–visible
analysis
dichloromethane
extracts
five
Streptomyces
sp.
Strains
that
showed
high
antioxidant
(ABTS
35.8%
DPPH
25.6%)
activities
revealed
absence
polyene
GC–MS
extract
E23-4
as
most
strain
presence
21
volatile
including
Pyrrolopyrazine
(98%)
Benzeneacetic
acid
(90%).
conclusion,
we
studied
isolation
strains
produce
with
a
microbiologically
unexplored
region
Morocco.
Furthermore,
study
has
demonstrated
significant
(
P
<
0.0001)
positive
correlation
between
total
phenolic
flavonoid
contents
capacity,
paving
way
characterization
these
optimal
use
anticancer,
antioxidant,
purposes.
Cell Genomics,
Journal Year:
2022,
Volume and Issue:
2(12), P. 100213 - 100213
Published: Nov. 11, 2022
The
phylum
Actinobacteria
includes
important
human
pathogens
like
Mycobacterium
tuberculosis
and
Corynebacterium
diphtheriae
renowned
producers
of
secondary
metabolites
commercial
interest,
yet
only
a
small
part
its
diversity
is
represented
by
sequenced
genomes.
Here,
we
present
824
actinobacterial
isolate
genomes
in
the
context
phylum-wide
analysis
6,700
including
public
isolates
metagenome-assembled
(MAGs).
We
estimate
that
30%–50%
projected
phylogenetic
possesses
genomic
representation
via
MAGs.
A
comparison
gene
functions
reveals
novel
determinants
host-microbe
interaction
as
well
environment-specific
adaptations
such
potential
antimicrobial
peptides.
identify
plasmids
prophages
across
uncover
extensive
prophage
structured
mainly
host
taxonomy.
Analysis
>80,000
biosynthetic
clusters
horizontal
transfer
loss
shape
metabolite
repertoire
taxa.
Our
observations
illustrate
essential
role
need
for
high-quality
genome
sequences.
