Angewandte Chemie International Edition,
Journal Year:
2021,
Volume and Issue:
60(29), P. 15803 - 15808
Published: April 30, 2021
Abstract
Quantitative
measurements
of
intravesicular
glutamate
(Glu)
and
transient
exocytotic
release
contents
directly
from
individual
living
neurons
are
highly
desired
for
understanding
the
mechanisms
(full
or
sub‐quantal
release?)
synaptic
transmission
plasticity.
However,
this
could
not
be
achieved
so
far
due
to
lack
adequate
experimental
strategies
relying
on
selective
sensitive
Glu
nanosensors.
Herein,
we
introduce
a
novel
electrochemical
nanobiosensor
based
single
SiC
nanowire
that
can
selectively
measure
in
real‐time
fluxes
released
via
exocytosis
by
large
vesicles
(ca.
125
nm
diameter)
present
hippocampal
axonal
varicosities
as
well
their
content
before
exocytosis.
These
revealed
mode
neurons,
viz.,
only
ca.
one
third
half
molecules
during
events.
Importantly,
fraction
remained
practically
same
when
were
pretreated
with
L‐Glu‐precursor
L‐glutamine,
while
it
significantly
increased
after
zinc
treatment,
although
both
cases
drastically
affected.
Biological Psychiatry,
Journal Year:
2016,
Volume and Issue:
81(10), P. 838 - 847
Published: May 22, 2016
Imbalances
between
excitation
and
inhibition
in
synaptic
transmission
neural
circuits
have
been
implicated
autism
spectrum
disorders.
Excitation
imbalances
are
frequently
observed
animal
models
of
disorders,
their
correction
normalizes
key
autistic-like
phenotypes
these
animals.
These
results
suggest
that
may
contribute
to
the
development
maintenance
disorders
represent
an
important
therapeutic
target.
Annual Review of Physiology,
Journal Year:
2016,
Volume and Issue:
78(1), P. 351 - 365
Published: Feb. 10, 2016
For
more
than
20
years,
we
have
known
that
Ca
2+
/calmodulin-dependent
protein
kinase
(CaMKII)
activation
is
both
necessary
and
sufficient
for
the
induction
of
long-term
potentiation
(LTP).
During
this
time,
tremendous
effort
has
been
spent
in
attempting
to
understand
how
CaMKII
gives
rise
phenomenon.
Despite
such
efforts,
there
much
be
learned
about
molecular
mechanisms
involved
LTP
downstream
activation.
In
review,
highlight
recent
developments
shaped
our
current
thinking
underlying
discuss
important
questions
remain
field.
Science,
Journal Year:
2016,
Volume and Issue:
353(6304), P. 1123 - 1129
Published: Sept. 8, 2016
Inhibitory
synapses
dampen
neuronal
activity
through
postsynaptic
hyperpolarization.
The
composition
of
the
inhibitory
postsynapse
and
mechanistic
basis
its
regulation,
however,
remain
poorly
understood.
We
used
an
in
vivo
chemico-genetic
proximity-labeling
approach
to
discover
proteins.
Quantitative
mass
spectrometry
not
only
recapitulated
known
proteins
but
also
revealed
a
large
network
new
proteins,
many
which
are
either
implicated
neurodevelopmental
disorders
or
unknown
function.
Clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
depletion
one
these
previously
uncharacterized
InSyn1,
led
decreased
sites,
reduced
frequency
miniature
currents,
increased
excitability
hippocampus.
Our
findings
uncover
rich
functionally
diverse
assemblage
that
regulate
inhibition
might
contribute
developmental
brain
disorders.
Science,
Journal Year:
2020,
Volume and Issue:
368(6496)
Published: June 11, 2020
Receptors
moving
in
and
out
of
the
synapse
The
number
neurotransmitter
receptors
their
spatial
organization
on
postsynaptic
site
is
a
central
determinant
synaptic
efficacy.
Sophisticated
techniques
to
visualize
track
movement
single
molecules
have
provided
us
with
profound
new
insights
into
these
dynamics.
We
now
know
that
undergo
movements
different
scales.
Groc
Choquet
review
our
present
understanding
mechanisms
regulate
glutamate
receptor
localization
clustering.
Receptor
are
fundamental
basic
function
participate
many
forms
plasticity.
Science
,
this
issue
p.
eaay4631
Signal Transduction and Targeted Therapy,
Journal Year:
2022,
Volume and Issue:
7(1)
Published: July 11, 2022
Autism
spectrum
disorder
(ASD)
is
a
prevalent
and
complex
neurodevelopmental
which
has
strong
genetic
basis.
Despite
the
rapidly
rising
incidence
of
autism,
little
known
about
its
aetiology,
risk
factors,
disease
progression.
There
are
currently
neither
validated
biomarkers
for
diagnostic
screening
nor
specific
medication
autism.
Over
last
two
decades,
there
have
been
remarkable
advances
in
genetics,
with
hundreds
genes
identified
as
being
associated
high
The
convergence
neuroscience
methods
becoming
more
widely
recognized
significance
elucidating
pathological
mechanisms
Efforts
devoted
to
exploring
behavioural
functions,
key
potential
treatments
Here,
we
highlight
this
review,
emerging
evidence
shows
that
signal
transduction
molecular
events
involved
processes
such
transcription,
translation,
synaptic
transmission,
epigenetics
immunoinflammatory
responses.
This
involvement
important
implications
discovery
precise
targets
Moreover,
review
recent
insights
into
clinical
autism
from
molecular,
cellular,
neural
circuit,
neurobehavioural
aspects.
Finally,
challenges
future
perspectives
discussed
regard
novel
strategies
predicated
on
biological
features
Science Translational Medicine,
Journal Year:
2023,
Volume and Issue:
15(689)
Published: March 29, 2023
Complement
overactivation
mediates
microglial
synapse
elimination
in
neurological
diseases
such
as
Alzheimer’s
disease
(AD)
and
frontotemporal
dementia
(FTD),
but
how
complement
activity
is
regulated
the
brain
remains
largely
unknown.
We
identified
that
secreted
neuronal
pentraxin
Nptx2
binds
C1q
thereby
regulates
its
brain.
Nptx2-deficient
mice
show
increased
activity,
C1q-dependent
engulfment,
loss
of
excitatory
synapses.
In
a
neuroinflammation
culture
model
aged
TauP301S
mice,
adeno-associated
virus
(AAV)–mediated
overexpression
was
sufficient
to
restrain
ameliorate
microglia-mediated
loss.
Analysis
human
cerebrospinal
fluid
(CSF)
samples
from
genetic
FTD
cohort
revealed
reduced
concentrations
Nptx2-C1q
protein
complexes
symptomatic
patients,
which
correlated
with
elevated
activated
C3.
Together,
these
results
diminished
might
exacerbate
complement-mediated
neurodegeneration
patients
FTD.
