Molecular and metabolic responses to immune stress in the jejunum of broiler chickens: transcriptomic and metabolomic analysis DOI Creative Commons
Weidong Hu,

Lin Du,

Jianjian Shao

et al.

Poultry Science, Journal Year: 2024, Volume and Issue: 103(5), P. 103621 - 103621

Published: March 6, 2024

In the large poultry industry, where farmed chickens are fed at high density, prevalence of pathogens and repeated vaccinations induce immune stress, which can significantly decrease production performance increase mortality. This study was designed to shed light on molecular mechanisms metabolic pathways involved in stress through an in-depth analysis transcriptomic metabolomic changes jejunum samples from broilers. Two groups were established for experiment: a control group LPS group. received intraperitoneal injection solution dose 250 μg per kg 12, 14, 33, 35 days age, whereas sterile saline injection. The severity assessed using Disease Activity Index. A jejunal section collected measure intestinal villus structure (villus length crypt depth). RNA sequencing metabolomics data conducted reveal differentially expressed genes metabolites. results showed that DAI index increased height/crypt depth decreased total 96 672 accumulating metabolites detected by compared comprehensive 23 enriched appetite, nutrient absorption, energy substance metabolism disorders ferroptosis play important role Our findings provide deeper understanding responses broilers LPS-induced suggesting potential targets therapeutic strategies improve broiler chickens.

Language: Английский

Ferroptosis in health and disease DOI Creative Commons
Carsten Berndt, Hamed Alborzinia,

Vera Skafar Amen

et al.

Redox Biology, Journal Year: 2024, Volume and Issue: 75, P. 103211 - 103211

Published: May 30, 2024

Ferroptosis is a pervasive non-apoptotic form of cell death highly relevant in various degenerative diseases and malignancies. The hallmark ferroptosis uncontrolled overwhelming peroxidation polyunsaturated fatty acids contained membrane phospholipids, which eventually leads to rupture the plasma membrane. unique that it essentially spontaneous, uncatalyzed chemical process based on perturbed iron redox homeostasis contributing process, but nonetheless modulated by many metabolic nodes impinge cells' susceptibility ferroptosis. Among affecting sensitivity, several have emerged as promising candidates for pharmacological intervention, rendering ferroptosis-related proteins attractive targets treatment numerous currently incurable diseases. Herein, current members Germany-wide research consortium focusing research, well key external experts who made seminal contributions this rapidly growing exciting field gathered provide comprehensive, state-of-the-art review Specific topics include: basic mechanisms, vivo relevance, specialized methodologies, tools, potential contribution disease etiopathology progression. We hope article will not only established scientists newcomers with an overview multiple facets ferroptosis, also encourage additional efforts characterize further molecular pathways modulating ultimate goal develop novel pharmacotherapies tackle associated - or caused

Language: Английский

Citations

75
Ferroptosis: Emerging mechanisms, biological function, and therapeutic potential in cancer and inflammation DOI Creative Commons
Xin Jin,

Jiuren Tang,

Xiangyu Qiu

et al.

Cell Death Discovery, Journal Year: 2024, Volume and Issue: 10(1)

Published: Jan. 24, 2024

Abstract Ferroptosis represents a distinct form of programmed cell death triggered by excessive iron accumulation and lipid peroxidation-induced damage. This mode differentiates from classical in terms morphology biochemistry. stands out for its exceptional biological characteristics has garnered extensive research conversations as death. Its dysfunctional activation is closely linked to the onset diseases, particularly inflammation cancer, making ferroptosis promising avenue combating these conditions. As such, exploring may offer innovative approaches treating cancer inflammatory diseases. Our review provides insights into relevant regulatory mechanisms ferroptosis, examining impact ferroptosis-related factors both physiological pathological perspectives. Describing crosstalk between tumor- inflammation-associated signaling pathways potential inducers overcoming drug-resistant cancers are discussed, aiming inform further novel therapeutic directions relation

Language: Английский

Citations

59
The Road from AKI to CKD: Molecular Mechanisms and Therapeutic Targets of Ferroptosis DOI Creative Commons
Runzhi Guo, Jiayu Duan, Shaokang Pan

et al.

Cell Death and Disease, Journal Year: 2023, Volume and Issue: 14(7)

Published: July 13, 2023

Abstract Acute kidney injury (AKI) is a prevalent pathological condition that characterized by precipitous decline in renal function. In recent years, growing body of studies have demonstrated maladaptation following AKI results chronic disease (CKD). Therefore, targeting the transition to CKD displays excellent therapeutic potential. However, mechanism mediated multifactor, and there still lack effective treatments. Ferroptosis, novel nonapoptotic form cell death, believed role progression. this study, we retrospectively examined history characteristics ferroptosis, summarized ferroptosis’s research progress CKD, discussed how ferroptosis participates regulating progression CKD. Furthermore, highlighted limitations present projected future evolution ferroptosis. We hope work will provide clues for further contribute study targets prevent diseases.

Language: Английский

Citations

47
Iron homeostasis in older adults: balancing nutritional requirements and health risks DOI Creative Commons
Rola S. Zeidan, Matthew Martenson, Javier Tamargo

et al.

The journal of nutrition health & aging, Journal Year: 2024, Volume and Issue: 28(5), P. 100212 - 100212

Published: March 14, 2024

Iron plays a crucial role in many physiological processes, including oxygen transport, bioenergetics, and immune function. is assimilated from food also recycled senescent red blood cells. exists two dietary forms: heme (animal based) non-heme (mostly plant based). The body uses iron for metabolic purposes, stores the excess mainly splenic hepatic macrophages. Physiologically, excretion humans inefficient not highly regulated, so regulation of intestinal absorption maintains homeostasis. losses occur at steady rate via turnover epithelium, loss, exfoliation dead skin cells, but overall homeostasis tightly controlled cellular systemic levels. Aging can have profound impact on induce dyshomeostasis where deficiency or overload (sometimes both simultaneously) occur, potentially leading to several disorders pathologies. To maintain physiologically balanced levels, reduce risk disease, promote healthy aging, it advisable older adults follow recommended daily intake guidelines periodically assess Clinicians evaluate status using different techniques selecting an assessment method primarily depends condition being examined. This review provides comprehensive overview forms, sources, metabolism iron, associated dyshomeostasis, levels adults, nutritional strategies balance adults.

Language: Английский

Citations

18
MiR-93-5p promotes granulosa cell apoptosis and ferroptosis by the NF-kB signaling pathway in polycystic ovary syndrome DOI Creative Commons
Wei Tan, Fangfang Dai, Dongyong Yang

et al.

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 19, 2022

Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in women reproductive age. miR-93-5p has been reported to be elevated granulosa cells PCOS patients. However, mechanism by which drives cell (GC) progression remains unclear. Thus, this study focuses on roles and mechanisms GCs PCOS. Methods KGN have similar ovarian physiological characteristics are used function regulatory GCs. In study, were transfected with si-NC, si-miR93-5p, oe-NC oe-miR93-5p. A counting kit-8 assay, flow cytometry western blotting performed observe proliferation apoptosis different groups. Subsequently, levels reactive oxygen species, malondialdehyde, GPX4, SLC7A11 Nrf2, indicators ferroptosis, measured a dihydroethidium fluorescent dye probe, biochemical kit, reverse transcription quantitative polymerase chain reaction. Ultimately, bioinformatic analysis experimental methods examine interaction between NF-κB signaling pathway. Results was upregulated Overexpression promoted ferroptosis cells, while knockdown showed effect. Biological subsequent experiments demonstrated that negatively regulates NF- κB Conclusion promotes GC regulating Silencing protects against dysfunction. Our identified as new molecular target for improving

Language: Английский

Citations

69
Targeting ferroptosis as a vulnerability in pulmonary diseases DOI Creative Commons
Li Yang, Limian Cao, Xiaoju Zhang

et al.

Cell Death and Disease, Journal Year: 2022, Volume and Issue: 13(7)

Published: July 26, 2022

Abstract Ferroptosis is an iron-dependent regulated cell death marked by excessive oxidative phospholipids (PLs). The polyunsaturated fatty acids-containing (PUFA-PLs) are highly susceptible to lipid peroxidation under stress. Numerous pulmonary diseases occurrences and degenerative pathologies driven ferroptosis. This review discusses the role of ferroptosis in pathogenesis including asthma, lung injury, cancer, fibrotic diseases, infection. Additionally, it proposed that targeting a potential treatment for particularly drug-resistant cancer or antibiotic-resistant infection, reduces treatment-related adverse events.

Language: Английский

Citations

60
Selenoprotein P – Selenium transport protein, enzyme and biomarker of selenium status DOI
Lutz Schomburg

Free Radical Biology and Medicine, Journal Year: 2022, Volume and Issue: 191, P. 150 - 163

Published: Sept. 5, 2022

Language: Английский

Citations

55
Dapagliflozin Ameliorates Renal Tubular Ferroptosis in Diabetes via SLC40A1 Stabilization DOI Creative Commons
Bin Huang, Wenjie Wen, Shandong Ye

et al.

Oxidative Medicine and Cellular Longevity, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 17

Published: Aug. 10, 2022

Tubular injury has been shown to play a critical role in the morbidity of diabetic kidney disease (DKD); ferroptosis often occurs tubules during renal development. This study was aimed at evaluating inhibitory effects and potential mechanism dapagliflozin (DAPA) against diabetic-related kidney. C57BL/6 mice were fed high-fat diet (HFD) for 12 weeks, administered small dose streptozocin (STZ) three consecutive days by intraperitoneal injection, then orally (10 mg/kg/day) 8 weeks. Mouse blood urine samples collected, their cortices harvested subsequent investigations. The DAPA also evaluated HK-2 cells subjected simulated conditions through excess glucose or palmitic acid (PA) administration. significantly ameliorated tubular independently glycemic control model mice. In vivo vitro investigations showed that inhibiting ferroptosis. Docking experiments demonstrated SLC40A1 could bind with each other may consequently reduce ubiquitination degradation. conclusion, this study, protective DAPA, irrespective control, observed mouse model. via stabilization, be underlying its action. To best our knowledge, is first investigate treatment DKD.

Language: Английский

Citations

41
Ferroptosis: Promising approach for cancer and cancer immunotherapy DOI
Shuyue Zheng,

Xin‐Yuan Guan

Cancer Letters, Journal Year: 2023, Volume and Issue: 561, P. 216152 - 216152

Published: April 4, 2023

Language: Английский

Citations

39
Diverse biological functions of vitamin K: from coagulation to ferroptosis DOI
Eikan Mishima, Adam Wahida,

Tobias Seibt

et al.

Nature Metabolism, Journal Year: 2023, Volume and Issue: 5(6), P. 924 - 932

Published: June 19, 2023

Language: Английский

Citations

35