Decoding the MMP14 Integrin Link: Key Player in the Secretome Landscape

Matrix Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Rapid progress has been made in the exciting field of secretome research health and disease. The tumor secretome, which is a significant proportion proteome, secreted into extracellular space to promote intercellular communication thus progression. Among many molecules integrins matrix metalloproteinase 14 (MMP14) stand out as interplay adhesion proteolysis drives invasion. Integrins serve mechanosensors that mediate contact cells with scaffold are significantly involved precise positioning activity control membrane-bound collagenase MMP14. As proteinase, MMP14 influences modifies itself. While MT-MMPs membrane bound, but can be released therefore border crossers between cell surface not constitutively cell-bound, its binding other receptors stringently regulated process. To understand mutual interactions detail, we first summarize structure function how it at enzymatic cellular level. In particular, include proteolytic cleavage themselves by We then review biochemical, biological physiological effects on composition associated functions when either bound membrane, or located microvesicles, proteolytically shed non-membrane-bound ectodomain. Novel methods proteomics, including analysis extravesicular vesicles, new for quantification will provide diagnostic tools. modification especially MMP14, may bring an additional aspect studies have impact diagnosis most likely also therapy cancer patients.

Language: Английский

Engineering cell-derived extracellular matrix for peripheral nerve regeneration

Materials Today Bio, Journal Year: 2024, Volume and Issue: 27, P. 101125 - 101125

Published: June 13, 2024

Extracellular matrices (ECMs) play a key role in nerve repair and are recognized as the natural source of biomaterials. In parallel to extensively studied tissue-derived ECMs (ts-ECMs), cell-derived (cd-ECMs) also have capability partially recapitulate complicated regenerative microenvironment native tissues. Notably, cd-ECMs can avoid shortcomings ts-ECMs. Cd-ECMs be prepared by culturing various cells or even autologous vitro under pathogen-free conditions. And mild decellularization achieve efficient removal immunogenic components cd-ECMs. Moreover, more readily customizable desired functional properties. These advantages garnered significant attention for potential neuroregenerative medicine. As promising biomaterials, bring new hope effective treatment peripheral injuries. Herein, this review comprehensively examines current knowledge about characteristics their mechanisms interaction with regeneration, particular focus on preparation, engineering optimization, scalability The applications from distinct cell sources reported tissue highlighted summarized. Furthermore, limitations that should addressed outlooks related clinical translation put forward well.

Language: Английский

Alterations in the microenvironment of junctional epidermolysis bullosa keratinocytes: a gene expression study

Matrix Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский