Benefits of physical exercise through multivariate analysis in sedentary adults and elderly: An analysis of physical fitness, health and anthropometrics

Experimental Gerontology, Journal Year: 2025, Volume and Issue: 200, P. 112669 - 112669

Published: Jan. 5, 2025

This study employed multivariate analysis to investigate the effects of a 14-week multicomponent training program on sedentary adults and older populations, focusing improvements in physical fitness, health markers, anthropometric measures. The data included 376 participants aged 30 84 years who had been inactive for at least 3 months. Results showed that 71 above lower systolic blood pressure, height, body mass, elbow flexion extension, sitting standing up from chair, agility, 6-Minute Walk Test (6MWT), handgrip strength values compared younger age groups (p < 0.05). Initially, 6MWT was inversely correlated with measures positively tests, but after intervention, it remained only agility. Interestingly, extension strongly other fitness pre-intervention, these relationships weakened afterward. Agility became key intermediary variable analysis. Despite improvements, variables like mass waist circumference continued have strong relationship outcomes. Significant gains were observed 0.05), indicating aerobic strength. Multivariate hip circumference, chair stand test, strength, agility significantly influenced performance. Using analysis, we able highlight importance incorporating regular exercise into daily life promote active healthy aging. Furthermore, emerged as most significant intermediate intervention. insight suggests may be crucial overall health.

Language: Английский

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14-Week exercise training modifies the DNA methylation levels at gene sites in non-Alzheimer's disease women aged 50 to 70 years

Experimental Gerontology, Journal Year: 2024, Volume and Issue: 186, P. 112362 - 112362

Published: Jan. 21, 2024

Exercise training emerges as a key strategy in lifestyle modification, capable of reducing the risk developing Alzheimer's disease (AD) due to factors such age, family history, genetics and low level education associated with AD. We aim analyze effect 14-week combined exercise (CT) on methylation genes AD non-alzheimer's women. CT sessions lasted 60 min, occurring three times week for 14 weeks. Forty non-Alzheimer's women aged 50 70 years (60.7 ± 4.1 years) mean height 1.6 0.1 m, weight 73.12 9.0 kg body mass index 29.69 3.5 kg/m2, underwent two physical assessments: pre post DNA assays utilized EPIC Infinium Methylation BeadChip from Illumina. observed that weeks led reductions systolic (p = 0.001) diastolic 0.017) blood pressure improved motor skills post-intervention. Among 25 linked AD, induced differentially methylated sites 12 genes, predominantly showing hypomethylated (negative β values). Interestingly, despite sites, some exhibited hypermethylated (positive values), ABCA7, BDNF, WWOX. A regimen was adequate induce differential CE-related healthy older women, alongside improvements pressure. In conclusion, this study suggest can be improve fitness individuals, especially able alterations related development It is important highlight should act protective factor adults.

Language: Английский

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Eight Weeks of Physical Training Decreases 2 Years of DNA Methylation Age of Sedentary Women

Research Quarterly for Exercise and Sport, Journal Year: 2023, Volume and Issue: 95(2), P. 405 - 415

Published: July 19, 2023

Purpose: The acceleration of epigenetic age is a predictor mortality and contributes to the increase in chronic diseases. Adherence healthy lifestyle strategy reduce age. present study aimed determine whether eight weeks combined (aerobic strength) training (CT) can influence women between 50 70 years old differences sites methylated regions. Methods: Eighteen (AARLow: lower residual, n = 10; AARHigh: higher 8) participated exercise program (60 minutes, 3× week) for weeks. DNA was extracted from whole blood using salting out technique. methylation performed array technique (Illumina's Infinium Methylation BeadChip 850k). We used Age Calculator platform calculate biological Two-way ANOVA followed by FISHER LSD posthoc Applied, adopting p < .05. Results: After CT, there were no changes AARLow group (PRE: -2.3 ± 3.2 POST: 3.6). However, AARHigh significantly decreased 3.6 2.6 2.2 2.7) (group effect, .01; time .31; vs. .005). Conclusion: CT benefits clock with most accelerated

Language: Английский

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Repetitive element transcript accumulation is associated with inflammaging in humans

GeroScience, Journal Year: 2024, Volume and Issue: 46(6), P. 5663 - 5679

Published: April 20, 2024

Language: Английский

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Resistance Exercise Training as a New Trend in Alzheimer’s Disease Research: From Molecular Mechanisms to Prevention

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 7084 - 7084

Published: June 27, 2024

Alzheimer's disease is a pathology characterized by the progressive loss of neuronal connections, which leads to gray matter atrophy in brain. most prevalent type dementia and has been classified into two types, early onset, associated with genetic factors, late environmental factors. One greatest challenges regarding high economic cost involved, why number studies aimed at prevention treatment have increased. possible approach use resistance exercise training, given that it shown neuroprotective effects disease, such as increasing cortical hippocampal volume, improving neuroplasticity, promoting cognitive function throughout life cycle. However, how training specifically prevents or ameliorates not fully characterized. Therefore, aim this review was identify molecular basis could prevent treat disease.

Language: Английский

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Combined exercise training decreases blood pressure in OLDER women with NOS3 polymorphism providing changes in differentially methylated regions (DMRs)

Epigenetics, Journal Year: 2024, Volume and Issue: 19(1)

Published: July 5, 2024

The mechanisms by which the ageing process is associated to an unhealthy lifestyle and how they play essential role in aetiology of systemic arterial hypertension have not yet been completely elucidated. Our objective investigate influence NOS3 polymorphisms [-786T > C (Glu298Asp)] on systolic blood pressure (SBP) diastolic (DBP) response, differentially methylated regions (DMRs), physical fitness adult older women after a 14-week combined training intervention. was carried out for 14 weeks, performed 3 times week, totalling 180 minutes weekly. genotyping experiment used Illumina Infinium Global Screening Array version 2.0 (GSA V2.0) Illumina's EPIC Methylation BeadChip. participants were separated into SNP rs2070744 TT (59.7 ± 6.2 years) TC + CC (60.0 5.2 years), rs17999 GluGlu (58.8 5.7 GluAsp AspAsp (61.6 4.9 years). We observed effect time variables BP, capacities, cholesterol. DMRs related SBP DBP identified groups pre- decreased numbers post-training. When we analysed exercise post-comparisons, (rs17999) showed 10 DMRs, enrichment, several biological biases. improved values regardless SNPs. In addition, affected DNA methylation differently between polymorphisms.

Language: Английский

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Older postmenopausal women with lower lean mass have hypermethylated sites in the PI3K-Akt pathway

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: April 10, 2023

Introduction: The decrease in lean mass is directly related to the loss of independence, muscle strength, and worse quality life over years. Although genetic determinants were well recognized, recent literature has been uncovering new epigenetic factors affecting state muscular tissue. This study aimed verify differences DNA methylation profile among Brazilian postmenopausal women aged 50–70 years according evaluation. Methods: A cross-sectional comprised 40 After K-means cluster analysis participants divided into two groups, Lower Lean Mass group with 20 (61.1 ± 4.6 years) Higher (60.7 3.2 years). was measured by dual-energy X-ray emission densitometry (DEXA). participants' extracted using Salting Out technique subsequently, Illumina 850k EPIC Infinium Methylation BeadChip performed obtain data. Results: We obtained 1,913 differentially methylated sites ( p ≤ 0.005 β &gt; 5% &lt; −5%) a total 979 genes between groups 0.005; −5% 5%). In addition, PI3K-Akt pathway had greatest power significance an FDR × 10 –3 . Conclusion: Our results demonstrate differentiation specific different genes, which have essential functions body composition energy metabolism, supporting future studies that aim relate epigenetics.

Language: Английский

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Exercise-induced redox modulation as a mediator of DNA methylation in health maintenance and disease prevention

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 213, P. 113 - 122

Published: Jan. 17, 2024

Language: Английский

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ARYANA-BS: Context-Aware Alignment of Bisulfite-Sequencing Reads

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 23, 2024

Abstract Motivation DNA methylation plays a crucial role in biological processes, including imprinting, development, inflammation, and several disorders, such as cancer. Bisulfite sequencing (BS) is the gold standard for single-base resolution measuring methylation. This process involves treating genomic with sodium bisulfite, followed by polymerase chain reaction (PCR), converting unmethylated cytosines to thymines (C T) guanines adenines (G A). However, aligning reads obtained through next-generation (NGS) of converted challenging due high number mismatches caused these conversions. Various BS aligners aim simplify read alignment conventional modifying reference genome and/or reads. Methods include three-letter wild-card alignment, each its limitations. work introduces novel aligner, ARYANA-BS, which departs from considering base alterations within engine. Leveraging well-established patterns different contexts, ARYANA-BS generates five indexes reference, aligns all indexes, selects hit minimum penalty. To further enhance accuracy, an optional EM step has been developed, incorporating probability information decision-making index penalty read. The presented approach seeks improve accuracy accounting intricacies diverse contexts. Results Our experimental results, based on both simulated real data, demonstrate that achieves state-of-the-art while maintaining competitive speed memory usage. Availability source code ARYANA simulator normal bisulfite-treated reads, SAM file analyzer used post processing penalties, test procedures benchmarking using are publicly available https://github.com/hnikaein/aryana . Contact [email protected] Supplementary data at Journal Name online.

Language: Английский

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DNA Methylation in Aortic Aneurysms of Different Localizations

Russian Journal of Genetics, Journal Year: 2024, Volume and Issue: 60(6), P. 701 - 715

Published: June 1, 2024

Language: Английский

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