Neuropeptide Modulation Enables Biphasic Internetwork Coordination via a Dual-Network Neuron DOI Creative Commons
Barathan Gnanabharathi, Savanna-Rae H. Fahoum, Dawn M. Blitz

et al.

eNeuro, Journal Year: 2024, Volume and Issue: 11(6), P. ENEURO.0121 - 24.2024

Published: June 1, 2024

Linked rhythmic behaviors, such as respiration/locomotion or swallowing/chewing, often require coordination for proper function. Despite its prevalence, the cellular mechanisms controlling of underlying neural networks remain undetermined in most systems. We use stomatogastric nervous system crab Cancer borealis to investigate internetwork coordination, due small, well-characterized feeding-related (gastric mill [chewing, ∼0.1 Hz]; pyloric [filtering food, ∼1 Hz]). Here, we between these during Gly 1 -SIFamide neuropeptide modulatory state. activates a unique triphasic gastric rhythm which typically pyloric-only LPG neuron generates dual pyloric-plus mill-timed oscillations. Additionally, exhibits shorter cycles rhythm-timed bursts, and longer IC, IC plus LG bursts. Photoinactivation revealed that is necessary shorten cycle period, likely through rectified electrical coupling pacemaker neurons. Hyperpolarizing current injections demonstrated although bursting enables only bursts are prolong period. Surprisingly, photoinactivation also eliminated prolonged cycles, without changing firing frequency burst duration, suggesting via synaptic inhibition LPG, indirectly slows pacemakers coupling. Thus, same dual-network directly conveys excitation from endogenous funnels enable one network alternately decrease increase period related network.

Language: Английский

Neuropeptide Modulation of Bidirectional Internetwork Synapses DOI
Savanna-Rae H. Fahoum, Dawn M. Blitz

Journal of Neurophysiology, Journal Year: 2024, Volume and Issue: 132(1), P. 184 - 205

Published: May 22, 2024

Neuromodulation can enable neurons to simultaneously coordinate with separate networks. Both recruitment into, and coordination with, a second network occur via modulation of internetwork synapses. Alternatively, intrinsic ionic currents. We find that the same neuropeptide previously determined modulate currents also modulates bidirectional synapses are typically ineffective. Thus, complementary modulatory peptide actions neuron into network.

Language: Английский

Citations

3
Neuropeptide Modulation Enables Biphasic Inter-network Coordination via a Dual-Network Neuron DOI Creative Commons
Barathan Gnanabharathi, Savanna-Rae H. Fahoum, Dawn M. Blitz

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 20, 2024

Abstract Linked rhythmic behaviors, such as respiration/locomotion or swallowing/chewing often require coordination for proper function. Despite its prevalence, the cellular mechanisms controlling of underlying neural networks remain undetermined in most systems. We use stomatogastric nervous system crab Cancer borealis to investigate inter-network coordination, due small, well characterized feeding-related (gastric mill [chewing, ∼0.1 Hz]; pyloric [filtering food, ∼1 Hz]). Here, we between these during Gly 1 -SIFamide neuropeptide modulatory state. activates a unique triphasic gastric rhythm which typically pyloric-only LPG neuron generates dual pyloric- plus mill-timed oscillations. Additionally, exhibits shorter cycles rhythm-timed bursts, and longer IC, IC LG bursts. Photoinactivation revealed that is necessary shorten cycle period, likely through rectified electrical coupling pacemaker neurons. Hyperpolarizing current injections demonstrated although bursting enables only bursts are prolong period. Surprisingly, photoinactivation also eliminated prolonged cycles, without changing firing frequency burst duration, suggesting via synaptic inhibition LPG, indirectly slows pacemakers coupling. Thus, same dual-network directly conveys excitation from endogenous funnels enable one network alternately decrease increase period related network. Significance Statement Related behaviors frequently exhibit yet coordinating not determined investigated two well-characterized crustacean feeding-associated neuropeptide-elicited find fast/slow shortens fast slow, intrinsically generated pacemakers, despite rectification favoring opposite direction. another slow-network phase, chemical funneled synapse. reinforces diminishes actions, enabling distinct frequencies faster across different phases slower rhythm.

Language: Английский

Citations

2
Neuropeptide Modulation of Bidirectional Internetwork Synapses DOI Creative Commons
Savanna-Rae H. Fahoum, Dawn M. Blitz

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 16, 2023

Abstract Oscillatory networks underlying rhythmic motor behaviors, and sensory complex neural processing, are flexible, even in their neuronal composition. Neuromodulatory inputs enable neurons to switch participation between networks, or participate multiple simultaneously. Neuromodulation of internetwork synapses can both recruit coordinate a switching neuron second network. We previously identified an example which is recruited into dual-network activity via peptidergic modulation intrinsic properties. now ask whether the same neuropeptide also modulates for coordination. The crab ( Cancer borealis ) stomatogastric nervous system contains two well-defined feeding-related (pyloric, food filtering, ∼1 Hz; gastric mill, chewing, ∼0.1 Hz). projection MCN5 uses Gly 1 -SIFamide pyloric-only LPG dual pyloric plus mill-timed bursting LPG’s Descending input not required coordinated rhythm, thus intra-network its network must underlie coordination among these neurons. However, mill have been documented. Using two-electrode voltage clamp recordings, we found that graded synaptic currents (LG, IC, DG) were primarily negligible saline, but enhanced by -SIFamide. Further, entrain each other during application, indicating bidirectional, functional connectivity. Thus, mediates through parallel actions, modulating properties it as shown here, bidirectional New Noteworthy be simultaneously with separate networks. Both recruitment into, with, occur synapses. Alternatively, ionic currents. find determined modulate typically ineffective. complementary modulatory peptide actions

Language: Английский

Citations

2
Neuropeptide Modulation Enables Biphasic Internetwork Coordination via a Dual-Network Neuron DOI Creative Commons
Barathan Gnanabharathi, Savanna-Rae H. Fahoum, Dawn M. Blitz

et al.

eNeuro, Journal Year: 2024, Volume and Issue: 11(6), P. ENEURO.0121 - 24.2024

Published: June 1, 2024

Linked rhythmic behaviors, such as respiration/locomotion or swallowing/chewing, often require coordination for proper function. Despite its prevalence, the cellular mechanisms controlling of underlying neural networks remain undetermined in most systems. We use stomatogastric nervous system crab Cancer borealis to investigate internetwork coordination, due small, well-characterized feeding-related (gastric mill [chewing, ∼0.1 Hz]; pyloric [filtering food, ∼1 Hz]). Here, we between these during Gly 1 -SIFamide neuropeptide modulatory state. activates a unique triphasic gastric rhythm which typically pyloric-only LPG neuron generates dual pyloric-plus mill-timed oscillations. Additionally, exhibits shorter cycles rhythm-timed bursts, and longer IC, IC plus LG bursts. Photoinactivation revealed that is necessary shorten cycle period, likely through rectified electrical coupling pacemaker neurons. Hyperpolarizing current injections demonstrated although bursting enables only bursts are prolong period. Surprisingly, photoinactivation also eliminated prolonged cycles, without changing firing frequency burst duration, suggesting via synaptic inhibition LPG, indirectly slows pacemakers coupling. Thus, same dual-network directly conveys excitation from endogenous funnels enable one network alternately decrease increase period related network.

Language: Английский

Citations

0