miRNA155-5P participated in DDX3X targeted regulation of pyroptosis to attenuate renal ischemia/reperfusion injury DOI Creative Commons
Yan Zhang,

Xinghua Lv,

Qian Fan

et al.

Aging, Journal Year: 2023, Volume and Issue: 15(9), P. 3586 - 3597

Published: May 4, 2023

Renal ischemia/reperfusion injury (IRI) induced pathological damage to renal microvessels and tubular epithelial cells through multiple factors. However, studies investigated whether miRNA155-5P targeted DDX3X attenuate pyroptosis were scarce.The expression of pyroptosis-related proteins (caspase-1, interleukin-1β (IL-1β), NOD-like receptor family pyrin domain containing 3 (NLRP3), IL-18) up-regulated in the IRI group. Additionally, miR-155-5p was higher group comparing with sham The inhibited by mimic more than other groups. DEAD-box Helicase X-Linked (DDX3X), NLRP3, caspase-1, IL-1β, IL-18, LDH, rates all H/R groups control These indicators negative (NC) group.Current findings suggested that decreased inflammation involved downregulating DDX3X/NLRP3/caspase-1 pathway.Using models mouse hypoxia-reoxygenation (H/R)-induced human proximal (HK-2 cells), we analyzed changes pathology factors correlated DDX3X. Real-time reverse transcription polymerase chain reaction (RT-PCR) detected miRNAs enzyme-linked immunosorbent assay (ELISA) used detect lactic dehydrogenase activity. StarBase luciferase assays examined specific interplay miRNA155-5P. In group, severe tissue damage, swelling, examined.

Language: Английский

Recent Advances in Models, Mechanisms, Biomarkers, and Interventions in Cisplatin-Induced Acute Kidney Injury DOI Open Access

Sara J. Holditch,

Carolyn N. Brown, Andrew M. Lombardi

et al.

International Journal of Molecular Sciences, Journal Year: 2019, Volume and Issue: 20(12), P. 3011 - 3011

Published: June 20, 2019

Cisplatin is a widely used chemotherapeutic agent to treat solid tumours, such as ovarian, head and neck, testicular germ cell. A known complication of cisplatin administration acute kidney injury (AKI). The development effective tumour interventions with reduced nephrotoxicity relies heavily on understanding the molecular pathophysiology cisplatin-induced AKI. Rodent models have provided mechanistic insight into In subsequent review, we provide detailed discussion recent advances in AKI phenotype, principal findings therapy, pre-clinical use rodent models. Cisplatin-induced murine faithfully develop gross manifestations clinical decreased function, increased expression tubular biomarkers, evident by histology. Pathways involved include apoptosis, necrosis, inflammation, oxidative stress, ultimately providing translational platform for testing therapeutic efficacy potential interventions. This review provides foundation laid our current pathophysiology.

Language: Английский

Citations

310
Nephrotoxicity and Renal Pathophysiology: A Contemporary Perspective DOI Open Access

Lillie Marie A. Barnett,

Brian S. Cummings

Toxicological Sciences, Journal Year: 2018, Volume and Issue: 164(2), P. 379 - 390

Published: June 21, 2018

The kidney consists of numerous cell types organized into the nephron, which is basic functional unit kidney. Any stimuli that induce loss these cells can damage and renal failure. cause failure be intrinsic or extrinsic. Extrinsic causes include cardiovascular disease, obesity, diabetes, sepsis, lung liver Intrinsic glomerular nephritis, polycystic fibrosis, tubular death, stones. plays a prominent role in mediating toxicity drugs, environmental pollutants natural substances. Drugs known to nephrotoxic several cancer therapeutics, drugs abuse, antibiotics, radiocontrast agents. Environmental target cadmium, mercury, arsenic, lead, trichloroethylene, bromate, brominated-flame retardants, diglycolic acid, ethylene glycol. Natural nephrotoxicants aristolochic acids mycotoxins such as ochratoxin, fumonisin B1, citrinin. There are common characteristics between mechanisms induced by extrinsic causes. This ground exists primarily due similarities molecular death. review summarizes current state field nephrotoxicity. It emphasizes integrating our understanding nephrotoxicity with pathological-induced Such approaches needed address major questions field, diagnosis, prognosis treatment both acute chronic failure, progression injury disease.

Language: Английский

Citations

203
The Pathophysiology of Sepsis-Associated AKI DOI Open Access
Shuhei Kuwabara, Eibhlin Goggins, Mark D. Okusa

et al.

Clinical Journal of the American Society of Nephrology, Journal Year: 2022, Volume and Issue: 17(7), P. 1050 - 1069

Published: June 28, 2022

Sepsis-associated AKI is a life-threatening complication that associated with high morbidity and mortality in patients who are critically ill. Although it clear early supportive interventions sepsis reduce mortality, less they prevent or ameliorate sepsis-associated AKI. This likely because specific mechanisms underlying attributable to not fully understood. Understanding these will form the foundation for development of strategies diagnosis treatment Here, we summarize recent laboratory clinical studies, focusing on critical factors pathophysiology AKI: microcirculatory dysfunction, inflammation, NOD-like receptor protein 3 inflammasome, microRNAs, extracellular vesicles, autophagy efferocytosis, inflammatory reflex pathway, vitamin D, metabolic reprogramming. Lastly, identifying molecular targets defining subphenotypes permit precision approaches prevention

Language: Английский

Citations

105
The role of Nrf2 in acute kidney injury: Novel molecular mechanisms and therapeutic approaches DOI
Wei Wei, Ning Ma,

Xiaoye Fan

et al.

Free Radical Biology and Medicine, Journal Year: 2020, Volume and Issue: 158, P. 1 - 12

Published: July 12, 2020

Language: Английский

Citations

63
Therapeutic potential of mangiferin against kidney disorders and its mechanism of action: A review DOI Creative Commons
Pei Teng Lum, Mahendran Sekar, Siew Hua Gan

et al.

Saudi Journal of Biological Sciences, Journal Year: 2021, Volume and Issue: 29(3), P. 1530 - 1542

Published: Nov. 16, 2021

There is a swing in research developments concerning the utilization of natural products as effective pharmacotherapeutic agents due to their comparatively lower toxicities than synthetic compounds. Among products, mangiferin C-glucosyl xanthonoid polyphenol with remarkable pharmacological activities. Emerging evidence indicates therapeutic benefits against various kidney disorders, including renal injury, diabetic nephropathy, fibrosis, hyperuricemic and lupus nephritis, experimental animal models. The induced antioxidant response resulting vital functions, such protection inflammation, inhibits cell apoptosis, activates autophagy, causes immunomodulation, regulates urate transporters modulates signalling pathways. purpose this review provide brief overview vitro/in vivo reno-protective effect underlying mechanism(s) protecting disorders. Understanding actions prominence its excellent potential managing Thus, addition review, in-silico molecular docking performed nuclear factor kappa B (NF-κB) soluble epoxide hydrolase (sEH) study mechanism action mangiferin. It believed that safe molecule. observed positive effects are attributed inhibition inflammation caused by NF-κB sEH upregulation oxidative stress activation. Studies on efficacy safety clinical trials further warranted confirm medicinal agent for disorders humans.

Language: Английский

Citations

31
The Emerging Role of Ubiquitin-Specific Protease 36 (USP36) in Cancer and Beyond DOI Creative Commons

Meng-Yao Niu,

Y. Liu, Jinjin Shi

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(5), P. 572 - 572

Published: May 12, 2024

The balance between ubiquitination and deubiquitination is instrumental in the regulation of protein stability maintenance cellular homeostasis. deubiquitinating enzyme, ubiquitin-specific protease 36 (USP36), a member USP family, plays crucial role this dynamic equilibrium by hydrolyzing removing ubiquitin chains from target proteins facilitating their proteasome-dependent degradation. multifaceted functions USP36 have been implicated various disease processes, including cancer, infections, inflammation, via modulation numerous events, gene transcription regulation, cell cycle immune responses, signal transduction, tumor growth, inflammatory processes. objective review to provide comprehensive summary current state research on roles different pathological conditions. By synthesizing findings previous studies, we aimed increase our understanding mechanisms underlying these diseases identify potential therapeutic targets for treatment.

Language: Английский

Citations

5
MOF-Based Platform for Kidney Diseases: Advances, Challenges, and Prospects DOI Creative Commons
Lier Deng, Manli Guo,

Yijun Deng

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(6), P. 793 - 793

Published: June 11, 2024

Kidney diseases are important that affect human health worldwide. According to the 2020 World Health Organization (WHO) report, kidney have become top 10 causes of death. Strengthening prevention, primary diagnosis, and action kidney-related is great significance in maintaining improving quality life. It increasingly challenging address clinical needs with present technologies for diagnosing treating renal illness. Fortunately, metal-organic frameworks (MOFs) shown promise diagnosis treatment diseases. This review summarizes research progress MOFs disease recent years. Firstly, we introduce basic structure properties MOFs. Secondly, focus on utilization In disease, usually designed as biosensors detect biomarkers related disease. can not only be used an effective adsorbent uremic toxins during hemodialysis but also a precise intelligent drug delivery carriers. They combined nano-chelation technology solve problem imbalance trace elements Finally, describe current challenges prospects

Language: Английский

Citations

5
MiR-21-3p Plays a Crucial Role in Metabolism Alteration of Renal Tubular Epithelial Cells during Sepsis Associated Acute Kidney Injury via AKT/CDK2-FOXO1 Pathway DOI Creative Commons

Zhuoyong Lin,

Zhongwei Liu, Xi Wang

et al.

BioMed Research International, Journal Year: 2019, Volume and Issue: 2019, P. 1 - 12

Published: May 16, 2019

Objective . Sepsis and associated acute kidney injury (SAKI) are determined to be closely related poor prognosis. Because the metabolic alterations of tubular epithelial cells (TECs) crucial for occurrence development SAKI, we carried out this present study identify metabolism changes TECs during SAKI relevant mechanisms. Methods Rat model rat cell line were used in our study. ELISA was determine serum cytokines levels. Protein expressions examined with Western-Blotting transcriptions RNAs qRT-PCR. ADP/ATP assay Oil Red O staining examine energy lipid metabolism, respectively. Dual-luciferase reporter interactions between miRNA specific proteins. Cell cycle arrest apoptosis flow cytometry. Results AKI induced 12 h after CLP. Energy reduced significantly while FOXO1 levels increased remarkably SAKI. The both AKT CDK2 mRNAs miR-21-3p expression remarkably. Both as direct targets miR-21-3p. Furthermore, by vitro experiments, it demonstrated that regulated via AKT/CDK2 AKT/CDK2-FOXO1 pathway regulations on arrest, TECs. Conclusions MiR-21-3p mediates fate manipulating pathway, is regulation

Language: Английский

Citations

31
How Acute Kidney Injury Contributes to Renal Fibrosis DOI
Li Yang

Advances in experimental medicine and biology, Journal Year: 2019, Volume and Issue: unknown, P. 117 - 142

Published: Jan. 1, 2019

Language: Английский

Citations

31
Multi-Omics Techniques Make it Possible to Analyze Sepsis-Associated Acute Kidney Injury Comprehensively DOI Creative Commons
Jiao Qiao, Liyan Cui

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: July 7, 2022

Sepsis-associated acute kidney injury (SA-AKI) is a common complication in critically ill patients with high morbidity and mortality. SA-AKI varies considerably disease presentation, progression, response to treatment, highlighting the heterogeneity of underlying biological mechanisms. In this review, we briefly describe pathophysiology SA-AKI, biomarkers, reference databases, available omics techniques. Advances technology allow for comprehensive analysis integration multiple provides an opportunity understand information flow behind disease. These approaches will drive shift current paradigms prevention, diagnosis, staging provide renal community significant advances precision medicine analysis.

Language: Английский

Citations

19