Biomedicine & Pharmacotherapy,
Journal Year:
2021,
Volume and Issue:
135, P. 111201 - 111201
Published: Feb. 1, 2021
This
study
aimed
at
creating
a
zebrafish
screening
model
for
diabetic
retinopathy,
and
evaluated
the
effects
of
aflibercept,
which
is
being
used
to
treated
retinopathy.
A
morphological
change
occurred
160
mM
glucose.
The
survival
hatching
rate
decreased
in
dose-dependent
manner.
In
130
glucose
group,
retinal
vessel
diameter
was
more
than
double
that
normal
group.
embryo
morphology
changed
200
μg/mL
400
aflibercept.
decrease
μg/mL.
Aflibercept
100
nontoxic
effective
dose
retinopathy
model.
expression
inflammatory
markers
increased
hyperglycemia.
But
inflammation
improved
by
aflibercept
eye.
model,
diameters
vessels
were
reduced
after
treatment
with
molecular
biological
histopathological
efficacy
confirmed.
can
serve
new
drug
candidates
Biomedicine & Pharmacotherapy,
Journal Year:
2024,
Volume and Issue:
171, P. 116160 - 116160
Published: Jan. 21, 2024
The
aggrandised
advancement
in
utility
of
advanced
day-to-day
materials
and
nanomaterials
has
raised
serious
concern
on
their
biocompatibility
with
human
other
biotic
members.
In
last
few
decades,
understanding
toxicity
these
been
given
the
centre
stage
research
using
many
vitro
vivo
models.
Zebrafish
(Danio
rerio),
a
freshwater
fish
member
minnow
family
garnered
much
attention
due
to
its
distinct
features,
which
make
it
an
important
frequently
used
animal
model
various
fields
embryology
toxicological
studies.
Given
that
fertilization
development
zebrafish
eggs
take
place
externally,
they
serve
as
excellent
organism
for
studying
early
developmental
stages.
Moreover,
possess
comparable
genetic
composition
humans
share
almost
70%
genes
mammals.
This
particular
become
increasingly
popular,
especially
research.
serves
link
between
studies
analysis
It
is
appealing
choice
vertebrate
research,
when
employing
high-throughput
methods,
small
size,
swift
development,
relatively
affordable
laboratory
setup.
enhanced
comprehension
pathobiology
drug
toxicity.
review
emphasizes
recent
developments
screening
assays,
new
insights
gained
about
drugs
through
assays.
Specifically,
cardio,
neural,
and,
hepatic
toxicology
inferred
by
applications
nanoparticles
have
highlighted.
Frontiers in Genetics,
Journal Year:
2021,
Volume and Issue:
12
Published: April 15, 2021
The
second
most
prevalent
neurodegenerative
disorder
in
the
elderly
is
Parkinson’s
disease
(PD).
Its
etiology
unclear
and
there
are
no
available
disease-modifying
medicines.
Therefore,
more
evidence
required
concerning
its
pathogenesis.
use
of
neurotoxin
1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
(MPTP)
basis
animal
models
PD.
MPTP
metabolized
by
monoamine
oxidase
B
(MAO
B)
to
MPP
+
induces
loss
dopaminergic
neurons
substantia
nigra
mammals.
Zebrafish
have
been
commonly
used
developmental
biology
as
a
model
organism,
but
owing
perfect
mix
properties,
it
now
emerging
for
human
diseases.
(
Danio
rerio
)
cheap
easy
sustain,
evolve
rapidly,
breed
transparent
embryos
large
amounts,
readily
manipulated
different
methods,
particularly
genetic
ones.
Furthermore,
zebrafish
vertebrate
species
mammalian
findings
obtained
from
may
be
applicable
than
those
derived
invertebrates
such
Drosophila
melanogaster
Caenorhabditis
elegans
.
resemblance
cannot
taken
granted,
however.
goal
present
review
article
highlight
promise
PD
model.
As
aminergic
structures,
mode
action,
PINK1
roles
mimic
mammalians,
seems
viable
studying
MAO,
however,
vary
two
types
MAO
benefits
unique
zebrafish,
ability
perform
large-scale
or
drug
screens,
should
exploited
future
experiments
utilizing
models.
International Journal of Molecular Sciences,
Journal Year:
2021,
Volume and Issue:
22(19), P. 10766 - 10766
Published: Oct. 5, 2021
Animal
models
of
human
neurodegenerative
disease
have
been
investigated
for
several
decades.
In
recent
years,
zebrafish
(Danio
rerio)
and
medaka
(Oryzias
latipes)
become
popular
in
pathogenic
therapeutic
studies
about
diseases
due
to
their
small
size,
the
optical
clarity
embryos,
fast
development,
suitability
large-scale
screening.
Following
emergence
a
new
generation
molecular
biological
technologies
such
as
reverse
forward
genetics,
morpholino,
transgenesis,
gene
knockout,
many
models,
Parkinson’s,
Huntington’s,
Alzheimer’s,
were
constructed
medaka.
These
proved
that
genes
are
functionally
conserved
relation
homologues,
so
they
exhibit
similar
phenotypes
beings.
Therefore,
fish
suitable
model
investigation
pathologic
mechanisms
screening
drugs
potential
therapy.
this
review,
we
summarize
modelling
years.
PLoS ONE,
Journal Year:
2017,
Volume and Issue:
12(11), P. e0188084 - e0188084
Published: Nov. 16, 2017
Egg
quality
is
a
complex
biological
trait
and
major
determinant
of
reproductive
fitness
in
all
animals.
This
study
delivered
the
first
proteomic
portraits
egg
zebrafish,
leading
biomedical
model
for
early
development.
batches
good
poor
quality,
evidenced
by
embryo
survival
24
h,
were
sampled
immediately
after
spawning
used
to
create
pooled
or
replicated
sample
sets
whose
protein
extracts
subjected
different
levels
fractionation
before
liquid
chromatography
tandem
mass
spectrometry.
Obtained
spectra
searched
against
zebrafish
proteome
database
detected
proteins
annotated,
categorized
quantified
based
on
normalized
spectral
counts.
Manually
curated
automated
enrichment
analyses
revealed
eggs
be
deficient
involved
synthesis
energy
lipid
metabolism,
some
vitellogenin
products
lectins,
have
surfeit
endo-lysosomal
activities,
autophagy,
apoptosis,
oncogene
products,
lectins
envelope
proteins.
Results
pathway
network
suggest
that
this
aberrant
profile
results
from
failure
oocytes
giving
rise
properly
transit
through
final
maturation,
implicated
Wnt
signaling
etiology
defect.
Quantitative
comparisons
abundant
versus
17
candidate
markers.
Thus,
clearly
linked
developmental
potential,
phenomenon
begs
further
investigation
elucidate
root
causes
presently
serious
intractable
problem
livestock
human
medicine.
