Overexpression of Nrf2 reverses ferroptosis induced by Arenobufagin in gastric cancer

Toxicology and Applied Pharmacology, Journal Year: 2024, Volume and Issue: 484, P. 116842 - 116842

Published: Feb. 1, 2024

Language: Английский

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Oxidation, Glycation, and Carbamylation of Salivary Biomolecules in Healthy Children, Adults, and the Elderly: Can Saliva Be Used in the Assessment of Aging?

Journal of Inflammation Research, Journal Year: 2022, Volume and Issue: Volume 15, P. 2051 - 2073

Published: March 1, 2022

Aging is inextricably linked to oxidative stress, inflammation, and posttranslational protein modifications. However, no studies evaluate oxidation, glycation, carbamylation of salivary biomolecules as biomarkers aging. Saliva collection non-invasive, painless, inexpensive, which are advantages over other biofluids.The study enrolled 180 healthy subjects divided into six groups according age: 6-13, 14-19, 20-39, 40-59, 60-79, 80-100 years. The number individuals was determined a priori based on our previous experiment (power the test = 0.8; α 0.05). Non-stimulated saliva plasma were collected from participants, in aging by colorimetric, fluorometric, ELISA methods.The have demonstrated that modifications proteins increase with age, manifested decreased total thiol levels increased carbonyl groups, glycation (Nε-(carboxymethyl) lysine, advanced end products (AGE)) (carbamyl-lysine) old individuals. Oxidative lipids (4-hydroxynonenal) nucleic acids (8-hydroxy-2'-deoxyguanosine (8-OHdG)) also age. Salivary redox correlate poorly their levels; however, AGE 8-OHdG generally reflect blood concentrations. In multivariate regression model, they predictor and, receiver operating characteristic (ROC) analysis, significantly differentiate children adolescents (under 15 years old) working-age population (15-64 years) older people (65 older).Salivary most excellent diagnostic utility assessing process. can be used body.

Language: Английский

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Significance of nitrosative stress and glycoxidation products in the diagnosis of COVID-19

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 22, 2024

Abstract Nitrosative stress promotes protein glycoxidation, and both processes can occur during an infection with the SARS-CoV-2 virus. Therefore, aim of this study was to assess selected nitrosative parameters glycoxidation products in COVID-19 patients convalescents relative healthy subjects, including reference severity symptoms. The diagnostic utility biomarkers also evaluated patients. involved 218 COVID-19, 69 convalescents, 48 subjects. (NO, S-nitrosothiols, nitrotyrosine) (tryptophan, kynurenine, N-formylkynurenine, dityrosine, AGEs) were measured blood plasma or serum use colorimetric/fluorometric methods. levels NO ( p = 0.0480), S-nitrosothiols 0.0004), nitrotyrosine 0.0175), kynurenine < 0.0001), N-formylkynurenine dityrosine AGEs 0.0001) significantly higher, whereas tryptophan fluorescence lower than control group. Significant differences analyzed observed different stages COVID-19. In turn, concentrations controls. ROC analysis revealed that be useful for diagnosing infections virus because they differentiate (KN: sensitivity—91.20%, specificity—92.00%; NFK: sensitivity—92.37%, AGEs: sensitivity—99,02%, specificity—100%) sensitivity—82.22%, specificity—84.00%; sensitivity—82,86%, specificity—86,00%; DT: sensitivity—100%, specificity—100%; AGE: from subjects high sensitivity specificity. are intensified after redox fluctuate disease. Circulating stress/protein have potential convalescents.

Language: Английский

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Roles of selenoprotein K in oxidative stress and endoplasmic reticulum stress under selenium deficiency in chicken liver

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Journal Year: 2022, Volume and Issue: 264, P. 109504 - 109504

Published: Nov. 12, 2022

Language: Английский

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Silver nanoparticles elevate mutagenesis of eukaryotic genomes

G3 Genes Genomes Genetics, Journal Year: 2023, Volume and Issue: 13(3)

Published: Jan. 13, 2023

Metal nanoparticles, especially silver, have been used in various medical scenarios, due to their excellent antimicrobial effects. Recent studies shown that AgNPs do not exert mutagenic effects on target bacteria, but the degree which they compromise eukaryotic genomes remains unclear. To study this, we evaluated of fission yeast Schizosaccharomyces pombe ATCC-16979, ∼23% genes are homologous human ones, at single-nucleotide resolution, and whole-genome scale by running 283 mutation accumulation lines for ∼260,000 cell divisions total. We also explored action mutagenesis mechanisms using differential gene-expression analysis based RNAseq. Upon treatment, genomic base-substitution rate S. four-fold degenerate sites increased 3.46×, small indels were prone occur regions simple sequence repeats. The G:C → T:A transversion was significantly increased, likely mostly from oxidative damage. Thus, addition potency, might pose slight genotoxicity threats possibly genomes, though a low magnitude.

Language: Английский

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The Antioxidative Role of Chaperone-Mediated Autophagy as a Downstream Regulator of Oxidative Stress in Human Diseases

Technology in Cancer Research & Treatment, Journal Year: 2022, Volume and Issue: 21

Published: Jan. 1, 2022

Chaperone-mediated autophagy (CMA) plays an important role in regulating a variety of cellular functions by selectively degrading damaged or functional proteins the cytoplasm. One processes which CMA participates is oxidative stress response. Oxidative regulates activity, while protects cells from damage oxidized and preventing accumulation excessive reactive oxygen species (ROS). Changes activity have been found many human diseases, also involved. Therefore, understanding interaction mechanism ROS will provide new targets for disease treatment. In this review, we discuss combatting during development different conditions, such as aging, neurodegeneration, liver infections, pulmonary disorders, cancers.

Language: Английский

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Effect of Normobaric Hypoxia on Alterations in Redox Homeostasis, Nitrosative Stress, Inflammation, and Lysosomal Function following Acute Physical Exercise

Oxidative Medicine and Cellular Longevity, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 18

Published: Feb. 25, 2022

Hypoxia is a recognized inducer of oxidative stress during prolonged physical activity. Nevertheless, previous studies have not systematically examined the effects normoxia and hypoxia acute exercise. The study aimed at evaluating relationship between enzymatic nonenzymatic antioxidant barrier, total antioxidant/oxidant status, nitrosative damage, inflammation, lysosomal function in different exercise protocols under hypoxia. Fifteen competitive athletes were recruited for study. They subjected to two types cycling with intensities durations: graded until exhaustion (GE) simulated 30 km individual time trial (TT). Both performed normoxic hypoxic (FiO2 = 16.5%) conditions. number subjects was determined based on our experiment, assuming test power 0.8 α 0.05. We demonstrated enhanced systems (GE: ↑ catalase (CAT), superoxide dismutase; TT: CAT) concomitant decrease plasma reduced glutathione. In exercising hypoxia, redox status shifted favor oxidation reactions oxidant ↓ ratio), leading increased oxidation/nitration proteins advanced protein products (AOPP), ischemia-modified albumin, 3-nitrotyrosine, S-nitrosothiols; AOPP) lipids malondialdehyde). Concentrations nitric oxide its metabolites (peroxynitrite) significantly higher exercisers an associated increase inflammatory mediators myeloperoxidase, tumor necrosis factor-alpha) exoglycosidase activity N-acetyl-β-hexosaminidase, β-glucuronidase). Our indicates that even single intensive session disrupts barrier leads damage systemic level. High-intensity alters homeostasis more than less intense (TT, near anaerobic threshold) longer duration (20.2 ± 1.9 min vs. 61.1 5.4 min-normoxia; 18.0 63.7 3.0 min-hypoxia), while exacerbates stress, dysfunction athletic subjects.

Language: Английский

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Evaluation of Pro/Antioxidant Imbalance in Blood of Women with Polycystic Ovary Syndrome Based on Determination of Oxidized Low-Density Lipoproteins and Ferric Reducing Ability of Plasma Values

Biomedicines, Journal Year: 2022, Volume and Issue: 10(7), P. 1564 - 1564

Published: June 30, 2022

We investigated selected pro/antioxidant parameters in a group of women with polycystic ovary syndrome (PCOS) divided according to age, body mass index (BMI), waist-to-hip ratio (WHR), homeostatic model assessment for insulin resistance (HOMA-IR) and quantitative sensitivity check (Quicki). chose oxidized low-density lipoproteins (oxLDL-C) as marker oxidative stress the ferric reducing ability plasma (FRAP) antioxidant status. In PCOS, values BMI, WHR, age concentration glucose significantly affected oxLDL-C FRAP values. correlated level whole who were sensitive (HOMA-IR < 2.0). There was negative relationship between Anti-Müllerian hormone both FRAP. Furthermore, value inversely luteinizing (LH), follicle-stimulating (FSH) androstenedione, whereas it positively LH/FSH ratio. The oxLDL are associated metabolic hormonal course PCOS.

Language: Английский

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Correlations between circulating methylmalonic acid levels and all-cause and cause-specific mortality among patients with diabetes

Frontiers in Nutrition, Journal Year: 2022, Volume and Issue: 9

Published: Nov. 29, 2022

Evidence regarding serum methylmalonic acid (MMA) levels and mortality in individuals with diabetes is limited. This study aimed to evaluate the correlation between MMA all-cause cause-specific deaths patients diabetes.This a population-based cohort based on data from both National Health Nutrition Examination Survey (NHANES) Death Index 1999 2014. We assessed association of concentrations using Cox proportional hazard models after adjusting for lifestyle, demographic factors, comorbidities.Among 3,097 participants, 843 mortalities occurred during median follow-up 4.42 years. There were 242 due cardiovascular disease (CVD) 131 cancer-associated deaths. After multivariate adjustment, elevated markedly correlated high risk all-cause, CVD-, cancer-related Each one-unit increase natural log-transformed level increased (2.652 times), CVD (3.153 (4.514). Hazard ratios (95% confidence intervals [CIs]) comparing participants < 120 ≥250 nmol/L 2.177 (1.421-3.336) mortality, 3.560 (1.809-7.004) 4.244 (1.537-11.721) cancer mortality.Higher significantly associated higher CVD, mortality. These findings suggest that maintaining lower status may diabetes.

Language: Английский

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Could circulating biomarkers of nitrosative stress and protein glycoxidation be useful in patients with gastric cancer?

Frontiers in Oncology, Journal Year: 2023, Volume and Issue: 13

Published: July 12, 2023

Background Nitrosative stress leads to protein glycoxidation, but both processes may be strongly related the cancer development. Therefore, aim of this study was assess nitrosative and glycoxidation products in patients with gastric comparison healthy controls. We are also first evaluate diagnostic utility markers respect histopathological classifications (TNM, Lauren’s Goseki’s classification) parameters such as histological type, differentiation grade, presence vascular or neural invasion, desmoplasia Helicobacter pylori infection. Methods The included 50 controls matched for sex age. were measured colorimetrically/fluorometrically plasma serum samples. Student’s t-test Mann-Whitney U-test used statistical analysis. Results NO, S-nitrosothiols, nitrotyrosine, kynurenine, N-formylkynurenine, dityrosine, AGE Amadori significantly increased whereas tryptophan fluorescence decreased compared control. useful diagnosis because they differentiate from individuals high sensitivity specificity. Some determined characterised by AUC value GC according parameters. Conclusions Gastric is associated enhanced circulating glycation. Although further research on a tissue model needed, plasma/serum biomarkers dependent tumour size, invasion depth, lymph node distant metastasis, Thus, stress/protein have potential significance patients.

Language: Английский

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