Tumor Necrosis Factor-α: The Next Marker of Stroke

Disease Markers, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 8

Published: Feb. 27, 2022

Although there is no shortage of research on the markers for stroke, to our knowledge, are clear that can meet needs clinical prediction and treatment. The inflammatory cascade a critical process persists functions throughout stroke process, ultimately worsening outcomes increasing mortality. Numerous factors, including tumor necrosis factor (TNF), involved in this process. These factors play dual role during their mechanisms complex. As one representatives, TNF primary regulator immune system plays an essential spread inflammation. In researches done over last few years, factor-alpha (TNF-α) has emerged as potential marker because its stroke. This review summarizes latest TNF-α explores therapeutic target.

Language: Английский

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Role of Polyphenols as Antioxidant Supplementation in Ischemic Stroke

Oxidative Medicine and Cellular Longevity, Journal Year: 2021, Volume and Issue: 2021(1)

Published: Jan. 1, 2021

Stroke is the second most common cause of death globally and leading in China. The pathogenesis cerebral ischemia injury complex, oxidative stress plays an important role fundamental pathologic progression damage ischemic stroke. Previous studies have preliminarily confirmed that should be a potential therapeutic target antioxidant as treatment strategy for Emerging experimental demonstrated polyphenols exert to play neuroprotection after This comprehensive review summarizes effects some polyphenols, which inhibition on reactive oxygen species generation

Language: Английский

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BIOFLAVONOIDS AS AGENTS FOR CORRECTING NITRO-OXIDATIVE STRESS AND SALIVARY GLAND FUNCTIONS IN RATS EXPOSED TO ALCOHOL DURING MODELED LIPOPOLYSACCHARIDE-INDUCED SYSTEMIC INFLAMMATORY RESPONSE

Wiadomości Lekarskie, Journal Year: 2022, Volume and Issue: 75(3), P. 685 - 690

Published: Jan. 1, 2022

The aim: To investigate the effects of bioflavonoids (curcumin, epigallocatechin-3-gallate and quercetin) on nitro-oxidative stress functions submandibular SGs in rats under alcohol exposure during SIR.Materials methods: studies were conducted 35 Wistar line weighing 205-220 g, divided into 5 groups seven animals each: 1st group, control group I, included receiving isotonic sodium chloride solution intragastrically twice a day; 2nd II, exposed to (in dose 24 mg/kg through gavage day) for last 2 weeks lipopolysaccharide (LPS)-induced SIR; 3rd, 4th 5th LPS-induced SIR, which also received bioflavonoids. ("Sigma-Aldrich, Inc.", USA) as following: curcumin daily 200 mg/kg), 40 quercetin respectively. SIR was induced by intraperitoneal administration Salmonella typhi LPS (during first week 0.4 μg/kg body weight administered 3 times week; next 7 experiment once week. formation superoxide anion radical (О2 -), activity NO-synthase - total (NOS), its constitutive inducible isoforms (cNOS, iNOS), concentration peroxynitrites S-nitrosothiols evaluated spectrophotometrically. assess functional status their homogenate we determined α-amylase (spectrophotometrically) aquaporin-5 (by enzyme-linked immunosorbent assay). gav-age with orogastric cannul.Results: When applying conditions NADH-induced .О2 production decreased yielded result II 36.8% administering curcumin, 34.5% epigallocatechin-3-gallate, 41.3% quercetin. NOS tissues inferior 42.8% relevant data (under administration), 33.7% administration) 46.6% administration); iNOS 47.0, 38.3 52.0%, Under 35.6% 37.4% 39.3% content lower 34.5, 31.1 35.3%, led changes tissues: values exceeded 40.4% 38.2% 34.1% those grew 2.66, 2.61 2.55 times, respectively.Conclusions: use combined 40% ethanol considerably limits development SGs. increases level cNOS coupling, improves enhancing aquaporin-5.

Language: Английский

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Effects of glycemic indicators on early neurological outcomes in patients with acute ischemic stroke treated with intravenous thrombolysis

World Journal of Diabetes, Journal Year: 2025, Volume and Issue: 16(3)

Published: Jan. 20, 2025

BACKGROUND Stress hyperglycemia (SH) is a common phenomenon that present in about 50% of patients with acute ischemic stroke (AIS). It thought to be main risk factor for poor functional outcome among AIS undergoing intravenous thrombolysis (IVT). AIM To investigate the predictive value glycemic indicators early neurological outcomes (ENOs) treated IVT. METHODS We retrospectively reviewed prospectively collected database who underwent IVT at Department Neurology, Second Affiliated Hospital Xuzhou Medical University, between January 2017 and June 2022. ENO included improvement (ENI) deterioration (END), defined as decrease or increase National Institutes Health Stroke Scale (NIHSS) score baseline 24 hours after analyzed associations [including admission (AH), fasting blood glucose (FBG), SH ratio (SHR)] all subgroups stratified by diabetes mellitus (DM). RESULTS A total 819 were included. Among these, AH was observed 329 (40.2%). Compared without AH, those more likely have higher prevalence DM (P < 0.001) hypertension = 0.031) presented NIHSS scores 0.001). During first IVT, END occurred 208 (25.4%) ENI 156 (19.0%). Multivariate mixed logistic regression analyses indicated independently associated [odds (OR): 1.744, 95% confidence interval (CI): 1.236-2.463; P 0.002]. Subjects classified into four groups representing quartiles. Q1, quartiles SHR (Q2: OR: 2.306, 95%CI: 1.342-3.960; 0.002) (Q3: 2.284, 1.346-3.876; (Q4: 3.486, 2.088-5.820; FBG 1.746, 1.045-2.917; 0.033) 2.436, 1.476-4.022; had significantly overall population. However, none found DM. CONCLUSION Our study demonstrated presence rather than during admission.

Language: Английский

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Salivary chemokines and growth factors in patients with ischemic stroke

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 12, 2025

Language: Английский

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The Influence of Oxidative Stress Markers in Patients with Ischemic Stroke

Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1130 - 1130

Published: Sept. 6, 2024

Stroke is the second leading cause of death worldwide, and its incidence rising rapidly. Acute ischemic stroke a subtype that accounts for majority cases has high mortality rate. An effective treatment to minimize damage brain's neural tissue by restoring blood flow decreased perfusion areas brain. Many reports have concluded both oxidative stress excitotoxicity are main pathological processes associated with stroke. Current measures protect brain against serious caused insufficient. For this reason, it important investigate antioxidant strategies reduce damage. This review focuses on studies assessing concentration biomarkers level antioxidants (enzymatic non-enzymatic) their impact clinical prognosis patients after Mechanisms related production ROS/RNS role in pathogenesis presented, as well new therapeutic aimed at reducing effects ischemia reperfusion.

Language: Английский

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Oxidative alterations in exfoliated oral mucosa cells of patients with major depressive disorder

Journal of Oral Biology and Craniofacial Research, Journal Year: 2025, Volume and Issue: 15(2), P. 256 - 261

Published: Feb. 10, 2025

Language: Английский

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Evaluation of Salivary pH, Calcium, Magnesium, Phosphorus, and Total Protein in 16 Dogs Before and After Supplementing Oral Buttermilk for 8 Weeks

Veterinary Ophthalmology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 23, 2025

ABSTRACT Objective Determine if feeding two teaspoons per ten pounds of body weight day Saco powdered buttermilk (SPB) for 8 weeks affects salivary pH (SpH), magnesium (Mg), phosphorus (Phos), calcium (Ca), or total protein (TP) in dogs. Animals Eighteen dogs were enrolled following owner consent. Dogs with severe dental disease that had been administered antibiotics the past 3 months excluded. Procedures Fasted saliva samples collected using Salivette tubes within same hour on Days 0, 1, 30, 31, 60, 61, 90, and 91. was measured immediately upon collection, then mailed laboratory analysis. SPB at a dose 1–60. Baseline complete blood counts serum chemistries run before collection Day 1. Results Sixteen completed study. Side effects included flatulence (2) loose stool (1). cotton swab ingestion occurred twice prior to change method securing during collection. Weight remained stable. There no significant SpH, TP, Ca, Phos statistically significant, linear increase Mg ( p = 0.0065). Conclusions Feeding did not significantly affect pH, protein, calcium, phosphorus. small concentration over study period return baseline 30 days discontinuation supplementation, significance which is unknown.

Language: Английский

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Oxidation, Glycation, and Carbamylation of Salivary Biomolecules in Healthy Children, Adults, and the Elderly: Can Saliva Be Used in the Assessment of Aging?

Journal of Inflammation Research, Journal Year: 2022, Volume and Issue: Volume 15, P. 2051 - 2073

Published: March 1, 2022

Aging is inextricably linked to oxidative stress, inflammation, and posttranslational protein modifications. However, no studies evaluate oxidation, glycation, carbamylation of salivary biomolecules as biomarkers aging. Saliva collection non-invasive, painless, inexpensive, which are advantages over other biofluids.The study enrolled 180 healthy subjects divided into six groups according age: 6-13, 14-19, 20-39, 40-59, 60-79, 80-100 years. The number individuals was determined a priori based on our previous experiment (power the test = 0.8; α 0.05). Non-stimulated saliva plasma were collected from participants, in aging by colorimetric, fluorometric, ELISA methods.The have demonstrated that modifications proteins increase with age, manifested decreased total thiol levels increased carbonyl groups, glycation (Nε-(carboxymethyl) lysine, advanced end products (AGE)) (carbamyl-lysine) old individuals. Oxidative lipids (4-hydroxynonenal) nucleic acids (8-hydroxy-2'-deoxyguanosine (8-OHdG)) also age. Salivary redox correlate poorly their levels; however, AGE 8-OHdG generally reflect blood concentrations. In multivariate regression model, they predictor and, receiver operating characteristic (ROC) analysis, significantly differentiate children adolescents (under 15 years old) working-age population (15-64 years) older people (65 older).Salivary most excellent diagnostic utility assessing process. can be used body.

Language: Английский

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Salivary Xanthine Oxidase as a Potential Biomarker in Stroke Diagnostics

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: May 6, 2022

Stroke is one of the most common cerebrovascular diseases. Despite significant progress in understanding stroke pathogenesis, cases are still increasing. Thus, laboratory biomarkers sought to allow rapid and non-invasive diagnostics. Ischemia-reperfusion injury an inflammatory process with characteristic cellular changes leading microvascular disruption. Several studies have shown that hyperactivation xanthine oxidase (XO) a major pathogenic factor contributing brain dysfunction. Given critical role XO complications, this study aimed evaluate activity enzyme its metabolic products saliva subjects. Thirty patients subacute phase were included study: 15 hemorrhagic ischemic stroke. The control group consisted 30 healthy subjects similar cerebral regarding age, gender, status periodontium, dentition, oral hygiene. number individuals was determined priori based on our previous experiment (power test = 0.8; α 0.05). material mixed non‐stimulated whole (NWS) stimulated (SWS). We showed activity, specific output significantly higher NWS than controls. Hydrogen peroxide uric acid levels also considerably patients. Using receiver operating curve (ROC) analysis, we demonstrated XO-specific distinguishes from (AUC: 0.764) controls 0.973) very high sensitivity specificity. Saliva collection stress-free, requires no specialized medical personnel, allows continuous monitoring patient’s condition through sampling multiple times per day. Salivary differentiates accuracy (100%) specificity (93.75%) between mild moderate cognitive decline (AUC 0.988). salivary assessment may be potential screening tool for comprehensive neuropsychological evaluation. To summarize, demonstrates utility differential diagnosis

Language: Английский

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