The underlying mechanism of calcium toxicity-induced autophagic cell death and lysosomal degradation in early stage of cerebral ischemia

Anatomy & Cell Biology, Journal Year: 2024, Volume and Issue: 57(2), P. 155 - 162

Published: April 29, 2024

Cerebral ischemia is the important cause of worldwide disability and mortality, that one obstruction blood vessels supplying to brain. In early stage, glutamate excitotoxicity high level intracellular calcium (Ca

Language: Английский

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Preclinical validation of human recombinant glutamate-oxaloacetate transaminase for the treatment of acute ischemic stroke

iScience, Journal Year: 2024, Volume and Issue: 27(11), P. 111108 - 111108

Published: Oct. 9, 2024

Language: Английский

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Alleviation of ischemic brain injury by exercise preconditioning is associated with modulation of autophagy and mitochondrial dynamics in cerebral cortex of female aged mice

Experimental Gerontology, Journal Year: 2023, Volume and Issue: 178, P. 112226 - 112226

Published: June 1, 2023

Evidence from clinical studies and preclinical supports that exercise preconditioning can not only reduce the risk of stroke but also improve brain tissue functional outcome after stroke. It has been demonstrated autophagy mitochondrial dynamics are involved in ischemic However, it is still unclear whether preconditioning-induced neuroprotection against associated with modulation dynamics. Although age sex interactively affect risk, incidence, outcome, based on young male animals most often used to explore role prevention In current study, we examined could modulate a ischemia reperfusion (I/R) model female aged mice. The results showed reduced infarct volume improved neurological deficits. Additionally, increased levels autophagy-related proteins LC3-II/LC3-I, LC3-II, p62, Atg7, mitophagy-related Bnip3L Parkin, as well fusion modulator Mfn2 fission Drp1 cortex mice at 12 h I/R were present. Our contribute better understanding for elderly.

Language: Английский

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Scientific Landscape of Oxidative Stress in Stroke: From a Bibliometric Analysis to an in-Depth Review

Neurochemical Research, Journal Year: 2023, Volume and Issue: 48(11), P. 3327 - 3348

Published: July 28, 2023

Language: Английский

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Hemiplegia in acute ischemic stroke: A comprehensive review of case studies and the role of intravenous thrombolysis and mechanical thrombectomy

Ibrain, Journal Year: 2024, Volume and Issue: 10(1), P. 59 - 68

Published: Jan. 13, 2024

Abstract Acute ischemic stroke is a significant health concern worldwide, often leading to long‐term disability and decreased quality of life. Rapid appropriate treatment crucial for achieving optimal outcomes in these patients. Intravenous thrombolysis (IVT) mechanical thrombectomy (MT) are two commonly used interventions acute stroke, but their effectiveness improving neurological symptoms functional patients with hemiplegia remains uncertain. The aim this work was evaluate the impact IVT MT within 4.5‐h time frame on hemiplegia. A systematic review conducted following Preferred Reporting Items Systematic Reviews Meta‐Analyses guidelines. Relevant studies that assessed an were included. Data extracted analyzed determine overall effects interventions. Most included case reports indicate positive terms symptom improvement recovery after receiving specified frame. However, heterogeneity among limited use due contraindications posed challenges determining most effective option. findings from demonstrate both led decrease National Institutes Health Stroke Scale scores, indicating symptoms. results highlight beneficial early thrombolytic status stroke.

Language: Английский

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The Activation of GABAAR Alleviated Cerebral Ischemic Injury via the Suppression of Oxidative Stress, Autophagy, and Apoptosis Pathways

Antioxidants, Journal Year: 2024, Volume and Issue: 13(2), P. 194 - 194

Published: Feb. 3, 2024

Ischemic stroke is a devastating disease leading to neurologic impairment. Compounding the issue very limited array of available interventions. The activation γ-aminobutyric acid (GABA) type A receptor (GABAAR) has been reported produce neuroprotective properties during cerebral ischemia, but its mechanism action not yet fully understood. Here, in rat model photochemically induced we found that muscimol, GABAAR agonist, modulated GABAergic signaling, ameliorated anxiety-like behaviors, and attenuated neuronal damage rats suffering ischemia. Moreover, improved brain antioxidant levels, reducing accumulation oxidative products, which was closely associated with NO/NOS pathway. Notably, inhibition autophagy markedly relieved insult caused by We further established an oxygen-glucose deprivation (OGD)-induced PC12 cell injury model. Both vivo vitro experiments demonstrated obviously suppressed regulating AMPK-mTOR Additionally, inhibited apoptosis through inhibiting Bax/Bcl-2 These data suggest exerts effects ischemia improving stress apoptosis. Our findings indicate serves as target for treating highlight GABAAR-mediated signaling

Language: Английский

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Z-Ligustilide: A Potential Therapeutic Agent for Atherosclerosis Complicating Cerebrovascular Disease

Biomolecules, Journal Year: 2024, Volume and Issue: 14(12), P. 1623 - 1623

Published: Dec. 18, 2024

Atherosclerosis (AS) is one of the major catalysts ischemic cerebrovascular disease, and death disease burden from AS its complications are increasing. Z-ligustilide (Z-LIG) a key active ingredient in Angelica sinensis (Oliv.) Diels Ligusticum chuanxiong Hort. In this paper, we first introduced LIG’s physicochemical properties pharmacokinetics. Then, reviewed Z-LIG’s intervention therapeutic mechanisms on complications. The Z-LIG include improving lipid metabolism, antioxidant anti-inflammatory effects, protecting vascular endothelium, inhibiting endothelial fibrosis, pathological thickening, plaque calcification. diseases complicated by AS, exerts practical neuroprotective effects stroke (IS), transient attack (TIA), dementia (VaD) through anti-neuroinflammatory, anti-oxidation, anti-neuronal apoptosis, protection blood-brain barrier, promotion mitochondrial division angiogenesis, improvement cholinergic activity, inhibition astrocyte proliferation, endoplasmic reticulum stress. This paper aims to provide basis for subsequent studies prevention treatment and, thus, promote development interventional drugs AS.

Language: Английский

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Tat-CCT2 Protects the Neurons from Ischemic Damage by Reducing Oxidative Stress and Activating Autophagic Removal of Damaged Protein in the Gerbil Hippocampus

Neurochemical Research, Journal Year: 2023, Volume and Issue: 48(12), P. 3585 - 3596

Published: Aug. 10, 2023

Language: Английский

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Identification of the relationship between biomarkers of autophagy, apoptosis and inflammation in the acute period of atherothrombotic ischemic stroke

Medical Immunology (Russia), Journal Year: 2023, Volume and Issue: 25(4), P. 939 - 946

Published: June 1, 2023

The postischemic inflammatory response plays a significant role in the pathogenesis of acute ischemic stroke (IS). It has been established that IS is accompanied by aseptic inflammation, which induces activation costimulatory molecules process innate immunity to brain tissue damage. constantly progressive destruction neuronal antigens contributes an increase volume lesion. Evidence continues accumulate indicating important NLRP3-mediated inflammation IS. shown autophagy involved cascade Many anti-inflammatory mechanisms mediated involve key autophagic proteins Beclin-1, LC3, and p62. Experimental studies have suppresses NLRP3 inflammation. Data on cross interactions between apoptosis are still controversial. aim study was evaluate relationship biomarkers autophagy, dynamics period atherothrombotic article presents results dynamic serum concentration LC3 p62, indicators Bcl-2 p53, pro-inflammatory cytokines IL-1β, TNFα, IL-8, IL-18 neuroinflammation. A statistically studied parameters comparison with control group. maximum noted 1 st day after development ischemia patients severe course disease. activity, biomarkers, some systemic moderate revealed. obtained confirm literature data involvement regulation response.

Language: Английский

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Relationship between biomarkers of autophagy and inflammation in acute period of ischemic stroke

Medical alphabet, Journal Year: 2024, Volume and Issue: 33, P. 37 - 43

Published: Jan. 19, 2024

Postischemic neuroinflammation is a critical pathophysiological process within the entire pattern of cerebral ischemia, spanning early injury and tissue repair. According to recent experimental data, autophagy involved in regulation neuroinflammation, influencing outcome acute period ischemic stroke (IS). Objective . To evaluate relationship between biomarkers inflammation indicators dynamics atherothrombotic IS. Materials methods 112 patients newly developed IS 56 donors (control group) were examined. Patients underwent dynamic clinical neurological examination on 1st, 7th 14th days from onset disease (magnetic resonance imaging, testing using NIHSS scale, modified Rankin scale). At same time intervals, blood was drawn for testing. The number active autophagosomes peripheral assessed by flow cytometry specific Cyto-ID dye. serum concentrations proinflammatory cytokines IL‑1β, IL‑8, IL‑18 (interleukins‑1β, -8, -18), TNFα (tumor necrosis factor-α), Beclin‑1, LC 3 p62 determined enzyme-linked immunosorbent assay analysis. C-reactive protein highly sensitive immunoturbidimetric method. Results A statistically significant increase studied parameters revealed compared control group. maximum observed 1st day, key (LC 3, Cyto-ID) – day after development ischemia. direct established level concentration inflammatory (CRP, IL‑18, TNF-α) Conclusions identified correlations indicate participation activated post-ischemic its involvement brain damage stages (days 1–7). results obtained confirm literature data influence disease.

Language: Английский

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