Traditional Chinese decoction Si Zhi Wan attenuates ovariectomy (OVX)-induced bone loss by inhibiting osteoclastogenesis and promoting apoptosis of mature osteoclasts DOI Creative Commons

Qingman He,

Kanghua Fu,

Huan Yao

et al.

Frontiers in Pharmacology, Journal Year: 2022, Volume and Issue: 13

Published: Sept. 13, 2022

Si Zhi Wan (SZW) is a traditional Chinese decoction used for osteoporosis treatment. Currently, the effect of SZW on ovariectomy (OVX)-induced bone loss and underlying mechanisms remain unknown. Herein, we investigated therapeutic explored in vitro vivo. An OVX-induced model was established After administration 8 weeks, rats were sacrificed, uterus weighted to calculate its index. The femur change pathologically evaluated using hematoxylin eosin (H&E) staining. mineral density observed by micro-CT. RAW264.7 cells activated receptor activator nuclear factor-κB ligand (RANKL) vitro. osteoclastogenesis tartrate-resistant acid phosphatase (TRAP) staining, Western blotting, RT-PCR. pro-apoptosis mature osteoclasts examined after induction osteoclast maturation. Finally, NF-κB pathway evaluated. Our results demonstrated that ameliorated rats. In addition, inhibited attenuated osteoclast-mediated resorption also promoted apoptosis osteoclasts. Mechanically, exerts effects suppressing pathway. Overall, these findings may be potentially effective alternative treatment osteoporosis.

Language: Английский

Panaxatriol saponin ameliorates myocardial infarction-induced cardiac fibrosis by targeting Keap1/Nrf2 to regulate oxidative stress and inhibit cardiac-fibroblast activation and proliferation DOI
Huan Yao,

Qingman He,

Cong Huang

et al.

Free Radical Biology and Medicine, Journal Year: 2022, Volume and Issue: 190, P. 264 - 275

Published: Aug. 14, 2022

Language: Английский

Citations

48
MK-886 protects against cardiac ischaemia/reperfusion injury by activating proteasome-Keap1-NRF2 signalling DOI Creative Commons

Kai-Na Shi,

Pang‐Bo Li,

Hui-Xiang Su

et al.

Redox Biology, Journal Year: 2023, Volume and Issue: 62, P. 102706 - 102706

Published: April 20, 2023

Oxidative stress is considered a key factor contributing to the initiation and development of cardiac injury following ischaemia‒reperfusion (I/R). Arachidonate 5-lipoxygenase (ALOX5) rate-limiting enzyme for leukotriene biosynthesis. MK-886 an inhibitor ALOX5 that exhibits anti-inflammatory antioxidant activities. However, significance in preventing I/R-mediated underlying mechanism remain unclear. Cardiac I/R model was produced by ligation/release left anterior descending artery. (20 mg/kg) administered intraperitoneally into mice at 1 24 h before I/R. Our results indicated treatment significantly attenuated contractile dysfunction decreased infarct area, myocyte apoptosis, oxidative accompanied with reduction Kelch-like ECH-associated protein (keap1) upregulation nuclear erythroid 2-related 2 (NRF2). Conversely, administration proteasome epoxomicin NRF2 ML385 greatly abrogated MK-886-mediated cardioprotection after injury. Mechanistically, enhanced expression immunoproteasome subunit β5i, which interacted keap1 its degradation, leading activation NRF2-dependent response improvement mitochondrial fusion-fission balance I/R-treated heart. In summary, our present findings could protect heart against highlight may represent promising therapeutic candidate ischaemic disease.

Language: Английский

Citations

32
Overview of Panax ginseng and its active ingredients protective mechanism on cardiovascular diseases DOI

Ziwei Zhou,

Meijing Li,

Zekuan Zhang

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 334, P. 118506 - 118506

Published: July 2, 2024

Language: Английский

Citations

13
Advancements and challenges in pharmacokinetic and pharmacodynamic research on the traditional Chinese medicine saponins: a comprehensive review DOI Creative Commons

Yuhan Ma,

Yongxia Zhao,

Mingxia Luo

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: May 7, 2024

Recent research on traditional Chinese medicine (TCM) saponin pharmacokinetics has revealed transformative breakthroughs and challenges. The multicomponent nature of TCM makes it difficult to select representative indicators for pharmacokinetic studies. clinical application saponins is limited by their low bioavailability short half-life, resulting in fluctuating plasma concentrations. Future directions should focus novel compounds utilizing colon-specific delivery osmotic pump systems enhance oral bioavailability. Optimizing drug combinations, such as ginsenosides with aspirin, shows therapeutic potential. Rigorous validation essential practical applications. This review emphasizes a era research, highlighting the need validation. pharmacokinetics, guided principles, are development, multidisciplinary approaches comprehensive understanding. provides theoretical basis new drugs supports rational medication.

Language: Английский

Citations

9
The mechanism of Se in regulating the proliferation and apoptosis of sheep Leydig cells through the miR-200a/NRF2 pathway DOI
Kexin Li, Xiaolei Wang,

Liang Ma

et al.

Theriogenology, Journal Year: 2025, Volume and Issue: 235, P. 103 - 113

Published: Jan. 7, 2025

Language: Английский

Citations

1
HACE1 protects against myocardial ischemia–reperfusion injury via inhibition of mitochondrial fission in mice DOI Creative Commons

Bang-Xia Liu,

Zheng Juan,

Zhan-Wei Tang

et al.

BMC Cardiovascular Disorders, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 3, 2025

HECT domain and Ankyrin repeat Containing E3 ubiquitin-protein ligase 1 (HACE1) has been found to be associated with mitochondrial protection. Mitochondrial damage is a critical contributor myocardial ischemia–reperfusion injury (I/RI). However, little known about the role of HACE1 in pathogenesis I/RI. Male C57BL6 mice knockout (KO) were subjected 30 min ischemia via ligation left anterior descending artery, followed by 0, 2, 6, or 24 h reperfusion. The evaluated for histopathological injury, serum troponin I (cTnI) levels, oxidative stress apoptosis cardiac function. Prior ischemia, Mdivi-1(1.2 mg/kg) vehicle was administered. study revealed that increased expression ischemia/reperfusion (I/RI), resulted more severe dysfunction during I/R(P < 0.05). group exhibited higher levels malondialdehyde (MDA), greater fission, dissipation membrane potential (MMP), leading compared wild-type I/R group(P On other hand, further reduced superoxide dismutase (SOD) activity myocardium(P 0.05), supporting findings. adverse effects almost completely eliminated pharmacological blockade dynamin-related protein (Drp1) inhibitor, Mdivi-1, which inhibits fission Collectively, our data show I/RI downregulation Drp1 activation, causing cardiomyocytes undergo cell death. Therefore, could promising therapeutic target treatment

Language: Английский

Citations

1
Activation of Nrf2 signaling: A key molecular mechanism of protection against cardiovascular diseases by natural products DOI Creative Commons
Xiaoyu Wu,

Jiajia Wei,

Yang Yi

et al.

Frontiers in Pharmacology, Journal Year: 2022, Volume and Issue: 13

Published: Dec. 8, 2022

Cardiovascular diseases (CVD) are a group of cardiac and vascular disorders including myocardial ischemia, congenital heart disease, failure, hypertension, atherosclerosis, peripheral artery rheumatic cardiomyopathies. Despite considerable progress in prophylaxis treatment options, CVDs remain leading cause morbidity mortality impose an extremely high socioeconomic burden. Oxidative stress (OS) caused by disequilibrium the generation reactive oxygen species plays crucial role pathophysiology CVDs. Nuclear erythroid 2-related factor 2 (Nrf2), transcription endogenous antioxidant defense systems against OS, is considered ideal therapeutic target for management Increasingly, natural products have emerged as potential source Nrf2 activators with cardioprotective properties may therefore provide novel tool CVD. Here, we present updated comprehensive summary naturally occurring that exert their effects suppression OS through activation signaling, aim providing useful insights development strategies exploiting products.

Language: Английский

Citations

31
Suxiao Jiuxin Pill alleviates myocardial ischemia–reperfusion injury through the ALKBH5/GSK3β/mTOR pathway DOI Creative Commons
Yiping Li, Ruixia Lu,

Zhenchao Niu

et al.

Chinese Medicine, Journal Year: 2023, Volume and Issue: 18(1)

Published: March 23, 2023

Many studies have shown effective protection from myocardial ischemia-reperfusion injury (MIRI) in animal models, but few, if any, treatments yielded a substantial reduction clinical. Several showed significant therapeutic effects for the Chinese patent medicine Suxiao Jiuxin Pill (SJP) MIRI, although specific molecular mechanisms remain undefined. Recently, increasing evidence indicates an important role m6A modification autophagy regulation and SJP has not been investigated this regard.In vivo experiments were performed Wistar rat MIRI model. In vitro assays conducted hypoxia/reoxygenation (H/R)-treated H9c2 cells. cells with ALKBH5 GSK3β silencing constructed by lentivirus transfection. TUNEL Annexin V/PI carried out apoptosis detection. Then, was detected EpiQuik RNA methylation quantification kit, GFP-RFP-LC3B used to observe dynamic changes autophagy. The autophagosome structure assessed Transmission electron microscopy. qPCR immunoblot mRNA protein analyses, receptively.SJP significantly mitigated rats, reducing infarct size apoptosis, improving left ventricular function. addition, inhibited through GSK3β/mTOR pathway rats. cultured cells, H/R- related autophagic activity pathway. Additionally, enhanced expression H/R cardiomyocytes, which is impaired modification. Interestingly, knockdown H/R-induced whereas reversed these effects. Further that deficiency are dependent H/R-treated After administration above alleviated, suggesting ALKBH5/GSK3β/mTOR cardiomyocytes. These of common its two main constituents, including tetra-methylpyrazine (TMP) borneol (BOR).SJP improves rats alleviates pathway, thanks major constituents TMP BOR.

Language: Английский

Citations

15
The pathogenesis and therapeutic strategies of heat stroke-induced myocardial injury DOI Creative Commons
Rui Xia, Meng Sun, Yuling Li

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 8, 2024

Heat stroke (HS) is a febrile illness characterized by an elevation in the core body temperature to over 40°C, accompanied central nervous system impairment and subsequent multi-organ dysfunction syndrome. In recent years, mortality rate from HS has been increasing as ambient temperatures continue rise each year. The cardiovascular plays important role pathogenesis process of HS, it functions one key for thermoregulation its stability associated with severity HS. Systemic inflammatory response endothelial cell damage constitute pivotal attributes other factors such ferroptosis, disturbances myocardial metabolism heat shock protein dysregulation are also involved tissue this review, comprehensively detailed description HS-induced injury provided. current treatment strategies promising therapeutic targets discussed.

Language: Английский

Citations

6
The Function of Circular RNAs in Myocardial Ischemia–Reperfusion Injury: Underlying Mechanisms and Therapeutic Advancement DOI
Kaiyuan Chen, Zhou Liu, Junhou Lu

et al.

Cardiovascular Drugs and Therapy, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 30, 2024

Language: Английский

Citations

5