Magnetic soft microrobots for erectile dysfunction therapy DOI Creative Commons
Shuting Wang, Zhenqing Wang, Zongshan Shen

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(49)

Published: Nov. 18, 2024

Erectile dysfunction (ED) is a major threat to male fertility and quality of life, mesenchymal stromal cells (MSCs) are promising therapeutic option. However, outcomes compromised by low MSC retention survival rates in corpus cavernosum tissue. Here, we developed an innovative magnetic soft microrobot comprising ultrasoft hydrogel microsphere embedded with nanoparticle chain for delivery. This design also features phenylboronic acid groups scavenging reactive oxygen species (ROS). With Young’s modulus less than 1 kPa, the adapts its shape within narrow blood vessels, ensuring uniform distribution MSCs cavernosum. Our findings showed that compared traditional injections, delivery (MSC-Rob) significantly enhanced survival. In both rat beagle ED models, MSC-Rob treatment accelerated repair tissue restored erectile function. Single-cell RNA sequencing (scRNA-seq) revealed facilitates nerve vessel regeneration increasing presence regenerative macrophages. Overall, our not only advances clinical application therapy but broadens scope microrobots other cell therapies.

Language: Английский

Pharmacokinetic characteristics of mesenchymal stem cells in translational challenges DOI Creative Commons
Yunlong Shan, Mengying Zhang,

Enxiang Tao

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: Sept. 13, 2024

Language: Английский

Citations

17
Targeting ferroptosis: opportunities and challenges of mesenchymal stem cell therapy for type 1 diabetes mellitus DOI Creative Commons
Le Dai, Qing Wang

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 4, 2025

Abstract Type 1 diabetes mellitus (T1DM) is characterized by progressive β-cell death, leading to loss and insufficient insulin secretion. Mesenchymal stem cells (MSCs) transplantation currently one of the most promising methods for replacement therapy. However, recent studies have shown that ferroptosis not only key mechanisms but also reasons extensive cell death within a short period time after MSCs transplantation. Ferroptosis new type regulated (RCD) iron-dependent accumulation lipid peroxides. Due weak antioxidant capacity β-cells, they are susceptible cytotoxic stimuli such as oxidative stress (OS), therefore ferroptosis. Transplanted extremely perturbations in their microenvironment, especially OS, which can weaken induce through In pathophysiological process T1DM, large amount reactive oxygen species (ROS) produced, causing OS. Therefore, targeting may be way protect β-cells improve therapeutic effect This review reviews research related MSCs, summarizes developed strategies help inhibit study aims understand mechanism transplantation, emphasize importance protecting improving survival function transplanted provide direction therapy T1DM future.

Language: Английский

Citations

1
MSC-EV-transmitted HSPA8 alleviates cisplatin-induced ovotoxicity by regulating the MGARP/PRDX2 axis DOI
Shenghui Zhang, Hyung Geun Song,

Mengyuan Chang

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140973 - 140973

Published: Feb. 1, 2025

Language: Английский

Citations

0
The promise of mesenchymal stromal/stem cells in erectile dysfunction treatment: a review of current insights and future directions DOI Creative Commons
Ayyub Patel, Alaa Shafie, Asma’a H. Mohamed

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 26, 2025

Erectile dysfunction is a common and multifactorial condition that significantly impacts men's quality of life. Traditional treatments, such as phosphodiesterase type 5 inhibitors (PDE5i), often fail to provide lasting benefits, particularly in patients with underlying health conditions. In recent years, regenerative medicine, stem cell therapies, has emerged promising alternative for managing erectile dysfunction. This review explores the potential mesenchymal stromal/stem cells (MSCs) their paracrine effects, including extracellular vesicles (EVs), treatment MSCs have shown remarkable promoting tissue repair, reducing inflammation, regenerating smooth muscle cells, offering therapeutic benefits models Clinical trials demonstrated positive outcomes improving function other clinical parameters. highlights promise MSC therapy dysfunction, discusses existing challenges, emphasizes need continued research refine these therapies improve long-term patient outcomes.

Language: Английский

Citations

0
Enzymatic Oxidants, Antioxidants, and Inflammatory Bowel Disease DOI Creative Commons
R. Steven Esworthy

Applied Biosciences, Journal Year: 2025, Volume and Issue: 4(2), P. 19 - 19

Published: April 1, 2025

The role of oxidants and antioxidants in inflammatory bowel disease (IBD) has been actively explored since the early 1980s, starting with respiratory burst neutrophils ischemia pathology. Since that time, enzymatic components contributing to pool reactive oxygen species, including superoxide, H2O2, lipid hydroperoxides, counteracting antioxidants—catalase, glutathione peroxidases (Gpx), peroxiredoxins (PRDX), superoxide dismutases, others—have fleshed out. My perspective on IBD is from balance or imbalance oxidant sources process. I will present evidence involvement antioxidant processes based, as much possible, my experiences Gpxs. This be discussed terms both immune system local systems. As Gpxs are generally selenium-dependent, possible deficiencies selenium uptake active impact Gpx expression explored. more recently introduced ferroptosis, an iron-dependent peroxidation-based pathological process, reviewed for its IBD.

Language: Английский

Citations

0
An update on the use of stem cell therapy for erectile dysfunction DOI Creative Commons
Mohamad Abou Chakra,

Hugo Bailly,

Fabian Klampke

et al.

Asian journal of urology, Journal Year: 2024, Volume and Issue: 11(4), P. 530 - 544

Published: March 11, 2024

The objective of this systematic review was to analyze animal and human trial data better understand the efficacy stem cell therapy (SCT) for erectile dysfunction (ED) obstacles that may encounter its application in field. We searched electronic databases, including PubMed Scopus, published studies with Medical Subject Heading (MeSH) "erectile dysfunction" (AND) "stem therapy" (OR) dysfunction' "clinical "sexual dysfunction". search limited English-language journals full papers only. initial resulted 450 articles, which 90 relevant our aims were included analysis. ED is a multifactorial disease. Current treatment options rely on pharmacotherapy as well surgical options. Patients have side effects or unsatisfactory results following use these SCT restore pathophysiological changes leading rather than treating symptoms. It has been evaluated models shown promising humans. Results confirm does improve function animals different types SC use. In humans, evidence showed results, but trials heterogeneous mainly by lack randomization small sample size. Many challenges could limit future research field, ethical dilemmas, regulation, patient recruitment, cost therapy, standardized regimen. Repairing possibly replacing diseased cells, tissues, organs eventually retrieving normal should always be goals any can only guaranteed SCT. potential successful ED, particularly patients who are resistant classic therapy. promote nerve regeneration vascular regeneration, not symptomatic treatment.

Language: Английский

Citations

3
Efficacy of adipose‐derived mesenchymal stem cell therapy in the treatment of chronic micro‐ and macrovascular complications of diabetes DOI Creative Commons
Agnieszka Mikłosz, Adrian Chabowski

Diabetes Obesity and Metabolism, Journal Year: 2023, Volume and Issue: 26(3), P. 793 - 808

Published: Dec. 11, 2023

Abstract Diabetes mellitus is a highly prevalent disease characterized by hyperglycaemia that damages the vascular system, leading to micro‐ (retinopathy, neuropathy, nephropathy) and macrovascular diseases (cardiovascular disease). There are also secondary complications of diabetes (cardiomyopathy, erectile dysfunction or diabetic foot ulcers). Stem cell‐based therapies have become promising tool targeting symptoms its chronic complications. Among all stem cells, adipose‐derived mesenchymal cells (ADMSCs) great importance because their abundance, non‐invasive isolation no ethical limitations. Characteristics make ADMSCs good candidates for therapy wide immunomodulatory properties paracrine activities through secretion an array growth factors, chemokines, cytokines, angiogenic factors anti‐apoptotic molecules. Besides, after transplantation, show ex vivo expansion capacity differentiation other cell types, including insulin‐producing cardiomyocytes, chondrocytes, hepatocyte‐like neurons, endothelial photoreceptor‐like astrocytes. Preclinical studies shown ADMSC‐based effectively improved visual acuity, ameliorated polyneuropathy ulceration, arrested development progression kidney disease, alleviated diabetes‐induced cardiomyocyte hypertrophy. However, despite positive results obtained in animal models, there still several challenges need be overcome before preclinical can translated into clinical applications. To date, trials ongoing using treatment complications, most them ulcers. This narrative review summarizes recent outcomes on usage long‐term both models trials.

Language: Английский

Citations

8
Transcriptomic profiling of human granulosa cells between women with advanced maternal age with different ovarian reserve DOI Creative Commons

Zhi-Cheng Jia,

Yongqian Li, Bowen Zhou

et al.

Journal of Assisted Reproduction and Genetics, Journal Year: 2023, Volume and Issue: 40(10), P. 2427 - 2437

Published: Aug. 17, 2023

Abstract Background Age-related diminished ovarian reserve (DOR) is not absolute. Some advanced maternal age (AMA) still have normal (NOR) and often show better pregnancy outcomes. Exploring the transcriptomic profile of granulosa cells (GCs) in AMA could lead to new ideas for mitigating age-related reserve. Aim This study aimed analyze GCs with different Results In total, 6273 statistically significant differential expression genes (DEGs) (|log2fc|> 1, q < 0.05) were screened from two groups, among which 3436 upregulated, 2837 downregulated DOR group. Through Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analysis, potential functions dysregulated or NOR predicted. The GO analysis revealed that DEGs mainly enriched obsolete oxidation–reduction process, mitochondrion, metal ion binding, ATP etc. KEGG above-mentioned ferroptosis, regulation actin cytoskeleton, oxidative phosphorylation, Meanwhile, verification mRNA levels possible involvement “ferroptosis” Conclusions From a clinical perspective, we presented first data showing between At same time, identified role ferroptosis AMA, providing biological basis studying aging improving outcomes AMA.

Language: Английский

Citations

4
Mitochondria-Rich Microvesicles Alleviate CNI ED by Transferring Mitochondria and Suppressing Local Ferroptosis DOI Creative Commons

Zhenkang Liang,

Zehong Chen, Chaowei Zhang

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 11745 - 11765

Published: Nov. 1, 2024

Erectile dysfunction (ED) frequently arises as a complication of pelvic surgeries, including rectal and prostate surgery, has no definitive cure. This study explored whether mitochondria-rich microvesicles (MVs) can be used to treat ED stemming from cavernous nerve injury (CNI) investigated its potential mechanisms. We isolated MVs mitochondria (MT) PC12. The apoptosis rate, mitochondrial membrane (MMP), reactive oxygen species (ROS), derived (mtROS), iron content, malondialdehyde (MDA) content endogenous antioxidant system activity corpus cavernosum smooth muscle cells (CCSMCs) cultured with MT were detected in vitro. In vivo, twenty-four male Sprague Dawley rats randomly divided into four groups: sham operation group CNI injected PBS, respectively. After fourteen days treatment, the erectile function was measured penile tissues collected for histological analysis. Subsequently, inhibition MV performed explore mechanism rescue experiment. CCSMCs, PC12-MVs PC12-MT successfully identified. culture, ROS, mtROS, MDA CCSMCs significantly decreased, while MMP activities increased. transplantation restore CNIED rats. experiment suggested that exerted above therapeutic effect by transferring within it. improve may play role anti-OS anti-ferroptosis at transplant site through efficient transfer mitochondria, providing treatment vehicle ED.

Language: Английский

Citations

0
Magnetic soft microrobots for erectile dysfunction therapy DOI Creative Commons
Shuting Wang, Zhenqing Wang, Zongshan Shen

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(49)

Published: Nov. 18, 2024

Erectile dysfunction (ED) is a major threat to male fertility and quality of life, mesenchymal stromal cells (MSCs) are promising therapeutic option. However, outcomes compromised by low MSC retention survival rates in corpus cavernosum tissue. Here, we developed an innovative magnetic soft microrobot comprising ultrasoft hydrogel microsphere embedded with nanoparticle chain for delivery. This design also features phenylboronic acid groups scavenging reactive oxygen species (ROS). With Young’s modulus less than 1 kPa, the adapts its shape within narrow blood vessels, ensuring uniform distribution MSCs cavernosum. Our findings showed that compared traditional injections, delivery (MSC-Rob) significantly enhanced survival. In both rat beagle ED models, MSC-Rob treatment accelerated repair tissue restored erectile function. Single-cell RNA sequencing (scRNA-seq) revealed facilitates nerve vessel regeneration increasing presence regenerative macrophages. Overall, our not only advances clinical application therapy but broadens scope microrobots other cell therapies.

Language: Английский

Citations

0