WITHDRAWN: Spirulina C-phycocyanin: extraction, purification, stabilization, and inhibitory potential of key enzymes rRelated to pancreas inflammation, carbohydrate digestion, and glucose metabolism DOI Creative Commons

Saber Abdelkader Saїdi,

Turki M. Al-Shaikh, Khaled Hamden

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 15, 2024

Abstract Background in recent years, it has become necessary to use natural food colorants protect against different diseases and disorders. The present study aimed purify, evaluate the stability, investigate effect of purified Spirulina C-Phycocyanin (CPC) on digestive metabolic enzymes linked hyperglycemia. Methods purification CPC was performed using column chromatography isolate compounds reversed-phase high performance liquid combined with diode array detector (DAD) ultraviolet spectroscopy (DAD-HPLC-UV) infrared Fourier transform (FTIR). Results findings this indicate that is stable at temperatures below 45°C a pH range 5 7, as well during storage 4°C. In diabetic rats, ingestion by rats suppressed key related inflammation pancreas, such 5-Lipoxygenase (5-LO), hyaluronidase (HAase), myeloperoxidase (MPO), NADPH oxidase (NOX2 activities) 48, 41, 75, 47%, respectively. Additionally, reduced pancreas thiobarbituric acid reactive substance rates (TBARS) 59%, stimulated level nitric oxide (NO) approximately 164%. Moreover, powerfully intestinal lipase, α-amylase, maltase, sucrose activities, significantly decreased glycosylated hemoglobin (HbA1c). Furthermore, glycogen phosphorylase (GP), Glycogen synthase (GS) regulated content liver rats. addition, supplementation modulated hepatic carbohydrate metabolism liver, hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PD), glucose-6-phosphatase (G6P), fructose-1,6-bisphosphatase (FBP), average water intake (AFI AWI), resulting decrease blood glucose 56% compared untreated Conclusion Overall, results research showed can be used functional coloring compound prevent various pathologies ulcers, oxidative stress, diseases.

Language: Английский

Natural Pigments Recovery from Food By-Products: Health Benefits towards the Food Industry DOI Creative Commons

Daniela Magalhães,

Ricardo Gonçalves, Cristina V. Rodrigues

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(14), P. 2276 - 2276

Published: July 19, 2024

Given the health risks associated with synthetic colorants, natural pigments have emerged as a promising alternative. These renewable choices not only provide benefits but also offer valuable technical and sensory properties to food systems. The effective application of however, requires optimization processing conditions, exploration new sources, development novel formulations ensure stability maintain their inherent qualities. Several pigment sources been explored achieve broad color range desired by consumers. purpose this review is explore current advances in obtention utilization derived from by-products, which possess health-enhancing are extracted through environmentally friendly methods. Moreover, provides insights into extraction processes, applications, bioactivities different types pigments.

Language: Английский

Citations

10
Antiobesity and antidiabetes effects of Cyperus rotundus rhizomes presenting protein tyrosine phosphatase, dipeptidyl peptidase 4, metabolic enzymes, stress oxidant and inflammation inhibitory potential DOI Creative Commons

Faiza I. A. Abdella,

Amani Toumi,

Sarra Boudriga

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(5), P. e27598 - e27598

Published: March 1, 2024

Diabetes is a significant global health concern that increases the vulnerability to various chronic illnesses. In view of this issue, current research aimed examine effects administering an extract derived from tubers Cyperus rotundus L (CrE) on obesity, type 1 diabetes, and liver-kidney toxicity. Through utilization HPLC-DAD analysis, it was discovered contained several components, including quercetin (47.8%), luteolin glucoside (17%), (7.56%), apigenin-7-glucoside (6.29%), naringinin (4.52%), seven others. vitro experiments they have demonstrated CrE effectively inhibited key digestive enzymes associated with obesity 2 such as DPP-4, PTP1B, lipase, α-amylase, evidenced by their respective IC50 values are about 23, 51,83, 67 μg/ml respectively. Furthermore, when diabetic rats were administered CrE, activity pancreatic linked inflammation, namely 5-lipoxygenase (5-LO), hyaluronidase (HAase), myeloperoxidase (MPO), significantly suppressed 48, 41, 75, 47%, Moreover, exhibited protective β-cells inhibiting formation thiobarbituric acid reactive substances (TBARS) 65% induction superoxide Dismutase (SOD), catalase (CAT) glutathione peroxidase (GPX) activities 62, 108, 112% respectively compared untreated rat. Additionally, intestinal, pancreatic, serum lipase α-amylase activities. rats, administration glycogen phosphorylase (GP) stimulated synthase (GS) 45 30%; increased liver content 45%. modulated hepatic involved in carbohydrate metabolism, hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PD), glucose-6-phosphatase (G6P), fructose-1,6-bisphosphatase (FBP). Notably, average food water intake (AFI AWI) treated reduced 15 16% those without any treatment. Therefore, study effectiveness preventing treating diabetes.

Language: Английский

Citations

6
Skin anti-aging potential of Launaea procumbens extract: Antioxidant and enzyme inhibition activities supported by ADMET and molecular docking studies DOI Creative Commons
Hanan Khojah, Shaimaa R. Ahmed,

Shahad Y. Alharbi

et al.

Saudi Pharmaceutical Journal, Journal Year: 2024, Volume and Issue: 32(7), P. 102107 - 102107

Published: May 25, 2024

Aging is a natural process that occurs in all living organisms. Particularly, the skin embodies aging since it serves as barrier between body and its surroundings. Previously, we reported wound healing effect of Launaea procumbens identified compounds therein. The study aims to explore anti-aging properties plant extract. To effect, antioxidant potential L. methanolic extract (LPM) was assessed using two complementary DPPH FRAP assays. enzyme inhibitory on collagenase, elastase, hyaluronidase, tyrosinase evaluated assess direct effects. Similarly, anti-inflammatory activity indirect effects via assessment cyclooxygenase-2 (COX-2) 5-lipoxygenase (5-LOX). In addition, ADMET molecular docking studies were performed interaction mechanisms LPM with target enzymes. demonstrated significant (IC50 = 29.08 µg/mL) (1214.67 µM FeSO4/g extract) Plant showed inhibition 52.68, 43.76, 31.031, 37.13 µg/mL, respectively). COX-2 5-LOX capacity IC50 values 8.635 10.851 respectively. revealed high bind active sites enzymes crucially involved process. analysis their good absorption, distribution, metabolism profiles, they found be safe well. Study findings suggest promising source for development compounds. This, turn, may facilitate incorporation into cosmetic formulations after further investigation.

Language: Английский

Citations

6
Bee bread shows therapeutic and protective effects by alleviating inflammation, oxidative stress, and apoptosis on acetic acid-induced gastric ulcer in rats DOI
Hatice Çolak, Esra Tansu Sarıyer, Meral Yüksel

et al.

Archives of Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 11

Published: Feb. 23, 2025

This study investigated bee bread's (BB) protective and therapeutic effects on acetic acid-(AA)-induced gastric ulcers via oxidative stress, DNA damage, inflammation, apoptosis. Rats were administered saline-(1ml) or BB-(0.5g/kg/day;1ml) by oral gavage once daily for 10-day following 80% AA-induced chronic ulceration in treatment group. Pretreatment group received saline BB before 3-day after ulcer induction. Stomachs of decapitated rats collected index, histological biochemical analyses. significantly reduced the index levels chemiluminescence, HMGB-1, IL-6, IL-1ß IL-8 pretreatment groups. In BB-pretreated group, MPO-(saline\BB, 39.9±3.7 U/g;22.2±2.2 U/g), caspase-3 (0.40±0.07 ng/g;0.18±0.01 ng/g) IFN-γ (15.46±1.76;9.51±1.95 decreased TNF-α (31.77±5.13;18.94±2.59 only BB-treated MDA, GSH, NRF-2, 8-OHdG remained unchanged. has demonstrated reducing ROS production, modulating inflammation

Language: Английский

Citations

0
Heterocyclic Betanin from Opuntia stricta var. Dillenii: Purification, Stability, and Effects on Carbohydrate Digestion, Absorption, Metabolism, and Enzyme Inhibition in Obese Rats with Type 2 Diabetes DOI
Walaa I. El-Sofany, Ahmed S. Alshammari,

Mona Zaheed Alshammari

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106569 - 106569

Published: April 1, 2025

Language: Английский

Citations

0
Protective effects of C-Phycocyanin from Spirulina platensis against pancreatic inflammation, lymphocyte infiltration, impaired carbohydrate digestion, and glucose metabolism dysregulation in diabetic rats DOI Creative Commons

Insaf Bahrini,

Muna O. Alamoudi,

Maha Alazami

et al.

Journal of Agriculture and Food Research, Journal Year: 2025, Volume and Issue: unknown, P. 101951 - 101951

Published: April 1, 2025

Language: Английский

Citations

0
Design of a new palladium(ii) halide complex as a bio-active material: synthesis, physico-chemical studies, DFT-computations and evaluation of anti-inflammatory, antioxidant and anti-gastric damage activities DOI Creative Commons

Sarra Bougossa,

Noureddine Mhadhbi,

Ali Ben Ahmed

et al.

RSC Advances, Journal Year: 2024, Volume and Issue: 14(25), P. 17413 - 17433

Published: Jan. 1, 2024

Colorless single crystals of the zero-dimensional hybrid compound, (C6H10N2)2[PdCl6]·2H2O were acquired through slow evaporation technique. The crystal structure was explored using SC-XRD, which demonstrates that material crystallizes in centrosymmetric space group P1̄ triclinic system. density functional theory method at B3LYP/Lan2mb basis set level employed to establish optimized geometry and vibrational frequencies title compound. An acceptable correspondence observed between results obtained calculation experimental data, including structure, IR spectra. optical characteristics revealed a direct band gap energy 2.35 eV, validating semiconductor this new material. suggest strong agreement with data validate involvement metal orbitals defining HOMO-LUMO boundary. Simultaneous TGA-DTA shows remains solid up 210 °C. Beyond these temperatures, gradual decomposition process occurs, extending 440 °C unfolding several steps. This entails liberation diverse compounds, encompassing organic molecules, chlorine ions, ultimately leading formation palladium oxide (PdO) as final product. When given rats gastric ulcers dose 100 mg kg-1, compounds inhibit key enzyme responsible for neutrophil infiltration myeloperoxidase (MPO) by 38.7%. compound also alleviates cellular damage induced free radicals, demonstrated notable 48.3% decrease thiobarbituric acid reactive substance rates (TBARS) compared untreated rats. Additionally, bring about substantial 30.6% reduction surface area ulcers.

Language: Английский

Citations

3
Exploring beetroot (Beta vulgaris L.) for diabetes mellitus and Alzheimer's disease dual therapy: in vitro and computational studies DOI Creative Commons
Oluwafemi Adeleke Ojo, Gideon Ampoma Gyebi,

Emmanuel Henry Ezenabor

et al.

RSC Advances, Journal Year: 2024, Volume and Issue: 14(27), P. 19362 - 19380

Published: Jan. 1, 2024

This study explored the flavonoid-rich extract of beetroot ( Beta vulgaris L.) for type 2 diabetes mellitus (T2D) and Alzheimer's disease (AD) dual therapy by using in vitro molecular simulation studies.

Language: Английский

Citations

2
Spirothiazolidine-Derivative on Silver Nanoparticles and Carbon Nanotubes: Evaluation of Antibacterial, Anti-Fungal, Anti-Inflammatory, Antioxidant and Gastroprotective Activities DOI Creative Commons
Walaa I. El-Sofany, E. M. S. Azzam, Salman Latif

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(7), P. 901 - 901

Published: July 5, 2024

This study aims to develop innovative heterocyclic nanocomposites incorporating silver nanoparticles (SNPs) for potential therapeutic applications targeting infections, gastric ulceration, inflammation, and oxidative damage. By synthesizing new derivatives of spiro-thiazolidine-carbonitrile (Py-ST-X) them into Ag (AgNPs) carbon nanotubes (CNTs), we have prepared Ag@Py-ST-X Ag@Py-ST-X@CNT nanocomposites, respectively. The physical properties these materials were studied using XRD, TEM, SEM, Zeta techniques. In our investigation involving rats with ulcers, observed noteworthy inhibitory effects on acid enzyme activity, specifically H+/K+ATPase, by Ag@Py-ST-NO2 Ag@Py-ST-Br demonstrating reductions 25 34%, respectively, compared untreated ulcers. Nanotubulation compounds further improved their efficacy 29 45%, Additionally, showed the most potent myeloperoxidase (MPO)-inhibitory 36 49% inhibition, nanotubulated versions reaching 44 53%. Moreover, Ag@Py-ST-NO2@CNT Ag@Py-ST-Br@CNT significant antioxidant reducing thiobarbituric reactive substances (TBARS) 35 51%, hydrogen peroxide (H2O2) levels 49 71%, These confirmed in surface area (GSA) 44% 52%, a decrease ulcer index (UI) from 80% 38%, an increase curative (CI) 19 55 62% following administration Ag@Py-ST-Br@CNT, Histological studies support findings, suggesting as promising candidates treating various disorders.

Language: Английский

Citations

1
Postharvest integration of prickly pear betalain-enriched gummies with different sugar substitutes for decoding diabetes type-II and skin resilience - in vitro and in silico study DOI

Deepak Mehta,

Kritika Kuksal,

Abhilasha Sharma

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 464, P. 141612 - 141612

Published: Oct. 10, 2024

Language: Английский

Citations

1