Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 15, 2024
Abstract
Background
in
recent
years,
it
has
become
necessary
to
use
natural
food
colorants
protect
against
different
diseases
and
disorders.
The
present
study
aimed
purify,
evaluate
the
stability,
investigate
effect
of
purified
Spirulina
C-Phycocyanin
(CPC)
on
digestive
metabolic
enzymes
linked
hyperglycemia.
Methods
purification
CPC
was
performed
using
column
chromatography
isolate
compounds
reversed-phase
high
performance
liquid
combined
with
diode
array
detector
(DAD)
ultraviolet
spectroscopy
(DAD-HPLC-UV)
infrared
Fourier
transform
(FTIR).
Results
findings
this
indicate
that
is
stable
at
temperatures
below
45°C
a
pH
range
5
7,
as
well
during
storage
4°C.
In
diabetic
rats,
ingestion
by
rats
suppressed
key
related
inflammation
pancreas,
such
5-Lipoxygenase
(5-LO),
hyaluronidase
(HAase),
myeloperoxidase
(MPO),
NADPH
oxidase
(NOX2
activities)
48,
41,
75,
47%,
respectively.
Additionally,
reduced
pancreas
thiobarbituric
acid
reactive
substance
rates
(TBARS)
59%,
stimulated
level
nitric
oxide
(NO)
approximately
164%.
Moreover,
powerfully
intestinal
lipase,
α-amylase,
maltase,
sucrose
activities,
significantly
decreased
glycosylated
hemoglobin
(HbA1c).
Furthermore,
glycogen
phosphorylase
(GP),
Glycogen
synthase
(GS)
regulated
content
liver
rats.
addition,
supplementation
modulated
hepatic
carbohydrate
metabolism
liver,
hexokinase
(HK),
glucose-6-phosphate
dehydrogenase
(G6PD),
glucose-6-phosphatase
(G6P),
fructose-1,6-bisphosphatase
(FBP),
average
water
intake
(AFI
AWI),
resulting
decrease
blood
glucose
56%
compared
untreated
Conclusion
Overall,
results
research
showed
can
be
used
functional
coloring
compound
prevent
various
pathologies
ulcers,
oxidative
stress,
diseases.
Journal of Chemistry and Nutritional Biochemistry,
Journal Year:
2023,
Volume and Issue:
4(2), P. 51 - 77
Published: Dec. 31, 2023
Betanin,
a
prominent
compound
found
in
beetroot,
however,
the
potential
protective
effects
of
this
against
gastric
damage
and
ulceration
have
yet
to
be
explored.
This
study
aims
investigate
impact
purified
beetroot
betanin
(Bet)
on
activities
inflammatory
enzymes
gene
expressions
associated
with
inflammation
tissues.
Gastric
ulcer
rats
were
induced
by
ethanol
at
dose
5ml/kg
body
weight.
Ulcerated
treated
or
omeprazole;
from
which
some
inflammation-related
genes
gastric-inflammation
investigated
using
RT-PCR
biochemical
analysis,
respectively.
Bet
effectively
suppressed
key
involved
mucosal
inflammation,
including
5-Lipoxygenase
(5-LO),
Hyaluronidase
(HAase),
myeloperoxidase
(MPO),
58%,
50%,
45%,
Docking
studies
showed
binding
catalytic
sites
regions
for
enzymes’
activities,
thus
explaining
inhibitory
capacity.
ingestion
decreased
expression
levels
genes,
factor-kappa
B
(NF-ϰB)
57%.
down-regulation
subsequently
led
reduction
inducible
nitric
oxide
synthase
(iNOS),
cyclooxygenase-2
(COX-2),
tumour
necrosis
factor-α
(TNF-α).
supressed
thiobarbituric
acid
reactive
substance
(TBARS)
rates
59%,
augmented
(NO)
level
107%
protected
tissues
ulceration.
revealed
gastro-protective
as
confirmed
surface
area
acidity
75.9%
40%,
respectively,
significant
increase
weight
mucus
curative
index
117%
74.5%,
In
conclusion,
these
outcomes
physiological
molecular-based
investigations
are
indicative
use
functional
food
additive
prevention
ulcer.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Feb. 7, 2024
Abstract
The
full
text
of
this
preprint
has
been
withdrawn,
as
it
was
submitted
in
error.
Therefore,
the
authors
do
not
wish
work
to
be
cited
a
reference.
Questions
should
directed
corresponding
author.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 15, 2024
Abstract
Background
in
recent
years,
it
has
become
necessary
to
use
natural
food
colorants
protect
against
different
diseases
and
disorders.
The
present
study
aimed
purify,
evaluate
the
stability,
investigate
effect
of
purified
Spirulina
C-Phycocyanin
(CPC)
on
digestive
metabolic
enzymes
linked
hyperglycemia.
Methods
purification
CPC
was
performed
using
column
chromatography
isolate
compounds
reversed-phase
high
performance
liquid
combined
with
diode
array
detector
(DAD)
ultraviolet
spectroscopy
(DAD-HPLC-UV)
infrared
Fourier
transform
(FTIR).
Results
findings
this
indicate
that
is
stable
at
temperatures
below
45°C
a
pH
range
5
7,
as
well
during
storage
4°C.
In
diabetic
rats,
ingestion
by
rats
suppressed
key
related
inflammation
pancreas,
such
5-Lipoxygenase
(5-LO),
hyaluronidase
(HAase),
myeloperoxidase
(MPO),
NADPH
oxidase
(NOX2
activities)
48,
41,
75,
47%,
respectively.
Additionally,
reduced
pancreas
thiobarbituric
acid
reactive
substance
rates
(TBARS)
59%,
stimulated
level
nitric
oxide
(NO)
approximately
164%.
Moreover,
powerfully
intestinal
lipase,
α-amylase,
maltase,
sucrose
activities,
significantly
decreased
glycosylated
hemoglobin
(HbA1c).
Furthermore,
glycogen
phosphorylase
(GP),
Glycogen
synthase
(GS)
regulated
content
liver
rats.
addition,
supplementation
modulated
hepatic
carbohydrate
metabolism
liver,
hexokinase
(HK),
glucose-6-phosphate
dehydrogenase
(G6PD),
glucose-6-phosphatase
(G6P),
fructose-1,6-bisphosphatase
(FBP),
average
water
intake
(AFI
AWI),
resulting
decrease
blood
glucose
56%
compared
untreated
Conclusion
Overall,
results
research
showed
can
be
used
functional
coloring
compound
prevent
various
pathologies
ulcers,
oxidative
stress,
diseases.
